Dramatic Improvement of Crystals of Large RNAs by Cation Replacement and Dehydration

SummaryCompared to globular proteins, RNAs with complex 3D folds are characterized by poorly differentiated molecular surfaces dominated by backbone phosphates, sparse tertiary contacts stabilizing global architecture, and conformational flexibility. The resulting generally poor order of crystals of large RNAs and their complexes frequently hampers crystallographic structure determination. We describe and rationalize a postcrystallization treatment strategy that exploits the importance of solvation and counterions for RNA folding. Replacement of Li+ and Mg2+ needed for growth of crystals of a tRNA-riboswitch-protein complex with Sr2+, coupled with dehydration, dramatically improved the resolution limit (8.5–3.2 Å) and data quality, enabling structure determination. The soft Sr2+ ion forms numerous stabilizing intermolecular contacts. Comparison of pre- and posttreatment structures reveals how RNA assemblies redistribute as quasi-rigid bodies to yield improved crystal packing. Cation exchange complements previously reported postcrystallization dehydration of protein crystals and represents a potentially general strategy for improving crystals of large RNAs

Dynamic binding of driven interfaces in coupled ultrathin ferromagnetic layers

We demonstrate experimentally dynamic interface binding in a system consisting of two coupled ferromagnetic layers. While domain walls in each layer have different velocity-field responses, for two broad ranges of the driving field, H, walls in the two layers are bound and move at a common velocity. The bound states have their own velocity-field response and arise when the isolated wall velocities in each layer are close, a condition which always occurs as H->0. Several features of the bound states are reproduced using a one dimensional model, illustrating their general nature.Comment: 5 pages, 4 figures, to be published in Physical Review Letter

Blockade of adenosine A2A receptors prevents protein phosphorylation in the striatum induced by cortical stimulation

©2006 Society for NeurosciencePrevious studies have shown that cortical stimulation selectively activates extracellular signal-regulated kinase 1/2 (ERK1/2) phosphorylation and immediate early gene expression in striatal GABAergic enkephalinergic neurons. In the present study, we demonstrate that blockade of adenosine A2A receptors with caffeine or a selective A2A receptor antagonist counteracts the striatal activation of cAMP– protein kinase A cascade (phosphorylation of the Ser845 residue of the glutamate receptor 1 subunit of the AMPA receptor) and mitogenactivated protein kinase (ERK1/2 phosphorylation) induced by the in vivo stimulation of corticostriatal afferents. The results indicate that A2A receptors strongly modulate the efficacy of glutamatergic synapses on striatal enkephalinergic neurons.This work was supported by the Intramural Research Program of the National Institutes of Health, National Institute on Drug Abuse, Department of Health and Human Services

On the virtualization and dynamic orchestration of satellite communication services

Key features of satellite communications such as wide-scale coverage, broadcast/multicast support and high availability, together with significant amounts of new satellite capacity coming online, anticipate new opportunities for satellite communications services as an integral part within upcoming 5G systems. To materialize these opportunities, satellite communications services have to be provisioned and operated in a more flexible, agile and cost-effective manner than done today. In this context, this paper describes a solution for the virtualization and dynamic orchestration of satellite communication services that builds on the introduction of Software Defined Networking (SDN) and Network Function Virtualization (NFV) technologies within the satellite ground segment systems. Along with the description of the main system architecture traits, the flowchart of a general procedure for the dynamic instantiation of virtualized satellite networks on top of a SDN/NFV-enabled satellite ground segment system is provided. The paper also presents experimental results for the dynamic customization of satellite network services through the implementation of a set of virtualized satellite network functions that can be orchestrated over general purpose open virtual platforms.Peer ReviewedPostprint (author's final draft

On-demand network slicing using SDN/NFV-enabled satellite ground segment systems

© 2018 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes,creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.This paper proposes an architecture framework for the realization of on-demand satellite network slicing that is built on the introduction of Software Defined Networking (SDN) and Network Function Virtualization (NFV) technologies. In this way, service delivery with satellite networks is shifted from a network for connectivity model to a network for service model with a high degree of service customization and adaptability, including satellite bandwidth on-demand. Under this framework, we study the resource orchestration of satellite network services by formulating the on-demand network slicing as an optimization problem that provides flexible service chaining and provisioning taking into account diversified service requirements. The objective is to determine the optimal resource allocation for supporting a satellite network slice that minimizes resources consumption while meeting service specification requirements such as the end-to-end delay.Peer ReviewedPostprint (author's final draft

The Structure and Function of Small Nucleolar Ribonucleoproteins

Eukaryotes and archaea use two sets of specialized ribonucleoproteins (RNPs) to carry out sequencespecific methylation and pseudouridylation of RNA, the two most abundant types of modifications of cellular RNAs. In eukaryotes, these protein–RNA complexes localize to the nucleolus and are called small nucleolar RNPs (snoRNPs), while in archaea they are known as small RNPs (sRNP). The C/D class of sno(s)RNPs carries out ribose- 20-O-methylation, while the H/ACA class is responsible for pseudouridylation of their RNA targets. Here, we review the recent advances in the structure, assembly and function of the conserved C/D and H/ACA sno(s)RNPs. Structures of each of the core archaeal sRNP proteins have been determined and their assembly pathways delineated. Furthermore, the recent structure of an H/ACA complex has revealed the organization of a complete sRNP. Combined with current biochemical data, these structures offer insight into the highly homologous eukaryotic snoRNPs

Distraction by deviant sounds: disgusting and neutral words capture attention to the same extent

Several studies have argued that words evoking negative emotions, such as disgust, grab attention more than neutral words, and leave traces in memory that are more persistent. However, these conclusions are typically based on tasks requiring participants to process the semantic content of these words in a voluntarily manner. We sought to compare the involuntary attention grabbing power of disgusting and neutral words using them as rare and unexpected auditory distractors in a cross-modal oddball task, and then probing the participants’ memory for these stimuli in a surprise recognition task. Frequentist and Bayesian analyses converged to show that, compared to a standard tone, disgusting and neutral auditory words produced significant but equivalent levels of distraction in a visual categorization task, that they elicited comparable levels of memory discriminability in the incidental recognition task, and that the participants’ individual sensitivity to disgust did not influence the results. Our results suggest that distraction by unexpected words is not modulated by their emotional valence, at least when these words are task-irrelevant and are temporally and perceptually decoupled from the target stimuliThis work was supported by Research Grants PSI2014-54261-P, PSI2015-63525-P, and PSI2015-65116-P from the Spanish Ministry of Science, Innovation and Universities (MICINN), the Spanish State Agency for Research (AEI) and the European Regional Development Fund (FEDER), as well as Grants 2017PFR-URV-B2-32 from the Universitat Rovira i Virgili, and GRC 2015/006 from (Xunta de Galicia). Fabrice B. R. Parmentier’s contract at the University of the Balearic Islands is co-financed by the MICINN’s program for the incentivization and permanent incorporation of doctors (2016 call, Ref IEDI-2016-00742). Fabrice B. R. Parmentier is also an Adjunct Senior Lecturer at the University of Western AustraliaS

Ultra deep sub-kpc view of nearby massive compact galaxies

Using Gemini North telescope ultra deep and high resolution (sub-kpc) K-band adaptive optics imaging of a sample of 4 nearby (z~0.15) massive (~10^{11}M_sun) compact (R<1.5 kpc) galaxies, we have explored the structural properties of these rare objects with an unprecedented detail. Our surface brightness profiles expand over 12 magnitudes in range allowing us to explore the presence of any faint extended envelope on these objects down to stellar mass densities ~10^{6} M_sun/kpc^{2} at radial distances of ~15 kpc. We find no evidence for any extended faint tail altering the compactness of these galaxies. Our objects are elongated, resembling visually S0 galaxies, and have a central stellar mass density well above the stellar mass densities of objects with similar stellar mass but normal size in the present universe. If these massive compact objects will eventually transform into normal size galaxies, the processes driving this size growth will have to migrate around 2-3x10^{10}M_sun stellar mass from their inner (R<1.7 kpc) region towards their outskirts. Nearby massive compact galaxies share with high-z compact massive galaxies not only their stellar mass, size and velocity dispersion but also the shape of their profiles and the mean age of their stellar populations. This makes these singular galaxies unique laboratories to explore the early stages of the formation of massive galaxies.Comment: Accepted for publication in ApJ Letter. Version revised to match the accepted versio