DETEKSI DISTRIBUSI WHITE SPOT SYNDROME VIRUS PADA BERBAGAI ORGAN UDANG VANAME (Litopenaeus vannamei)

One way to detect the presence of the White Spot Syndrome Virus in several organs Vaname shrimp(Litopenaeus vannamei) were infected with the method of Polymerase Chain Reaction (PCR). WSSV virus isknown to attack various target organs, but it is difficult to detect early on shrimp. Therefore, this study aims todetect the presence of the distribution of White Spot Syndrome Virus in several organs Vaname shrimp(Litopenaeus vannamei) were infected with the Polymerase Chain Reaction method in order to early detectionefforts for the prevention and treatment of diseases caused by the virus. Organ observed that the swimming legs,gills, stomach and antennal gland. Research stages include sample preparation and DNA extraction, DNAamplification and electrophoresis. DNA extraction was performed using the method DTAB and CTAB-DNAamplification is done by using nested PCR. The emergence of DNA bands is an indicator of the presence ofWSSV in shrimp Vaname several organs which are then presented desktriptif. The results showed four WSSVinfected organs were observed. Based on the appearance, the entire sample of the swimming leg and antennalgland positively infected, then successively gills and stomach. The swimming leg can be used to diagnose thedisease early on shrimp WSSV Vaname without turning off the shrimp.Keywords : Distribution, Vanname shrimp, WSSV, PC

Lessons learned from the introduction of autonomous monitoring to the EUVE science operations center

The University of California at Berkeley's (UCB) Center for Extreme Ultraviolet Astrophysics (CEA), in conjunction with NASA's Ames Research Center (ARC), has implemented an autonomous monitoring system in the Extreme Ultraviolet Explorer (EUVE) science operations center (ESOC). The implementation was driven by a need to reduce operations costs and has allowed the ESOC to move from continuous, three-shift, human-tended monitoring of the science payload to a one-shift operation in which the off shifts are monitored by an autonomous anomaly detection system. This system includes Eworks, an artificial intelligence (AI) payload telemetry monitoring package based on RTworks, and Epage, an automatic paging system to notify ESOC personnel of detected anomalies. In this age of shrinking NASA budgets, the lessons learned on the EUVE project are useful to other NASA missions looking for ways to reduce their operations budgets. The process of knowledge capture, from the payload controllers for implementation in an expert system, is directly applicable to any mission considering a transition to autonomous monitoring in their control center. The collaboration with ARC demonstrates how a project with limited programming resources can expand the breadth of its goals without incurring the high cost of hiring additional, dedicated programmers. This dispersal of expertise across NASA centers allows future missions to easily access experts for collaborative efforts of their own. Even the criterion used to choose an expert system has widespread impacts on the implementation, including the completion time and the final cost. In this paper we discuss, from inception to completion, the areas where our experiences in moving from three shifts to one shift may offer insights for other NASA missions

Deteksi Distribusi White Spot Syndrome Virus pada Berbagai Organ Udang Vaname (Litopenaeus Vannamei)

One way to detect the presence of the White Spot Syndrome Virus in several organs Vaname shrimp(Litopenaeus vannamei) were infected with the method of Polymerase Chain Reaction (PCR). WSSV virus isknown to attack various target organs, but it is difficult to detect early on shrimp. Therefore, this study aims todetect the presence of the distribution of White Spot Syndrome Virus in several organs Vaname shrimp(Litopenaeus vannamei) were infected with the Polymerase Chain Reaction method in order to early detectionefforts for the prevention and treatment of diseases caused by the virus. Organ observed that the swimming legs,gills, stomach and antennal gland. Research stages include sample preparation and DNA extraction, DNAamplification and electrophoresis. DNA extraction was performed using the method DTAB and CTAB-DNAamplification is done by using nested PCR. The emergence of DNA bands is an indicator of the presence ofWSSV in shrimp Vaname several organs which are then presented desktriptif. The results showed four WSSVinfected organs were observed. Based on the appearance, the entire sample of the swimming leg and antennalgland positively infected, then successively gills and stomach. The swimming leg can be used to diagnose thedisease early on shrimp WSSV Vaname without turning off the shrimp

Lens epithelial cell apoptosis and intracellular Ca(2+) increase in the presence of xanthurenic acid

BACKGROUND: Xanthurenic acid is an endogenous product of tryptophan degradation by indoleamine 2,3-dioxygenase (IDO). We have previously reported that IDO is present in mammalian lenses, and xanthurenic acid is accumulated in the lenses with aging. Here, we studied the involvement of xanthurenic acid in the human lens epithelial cell physiology. METHODS: Human lens epithelial cells primary cultures were used. Control cells, and cells in the presence of xanthurenic acid grow in the dark. Western blot analysis and immunofluorescence studies were performed. RESULTS: In the presence of xanthurenic acid human lens epithelial cells undergo apoptosis-like cell death. In the control cells gelsolin stained the perinuclear region, whereas in the presence of 10 μM xanthurenic acid gelsolin is translocated to the cytoskeleton, but does not lead to cytoskeleton breakdown. In the same condition caspase-3 activation, and DNA fragmentation was observed. At low (5 to 10 μM) of xanthurenic acid concentration, the elongation of the cytoskeleton was associated with migration of mitochondria and cytochrome c release. At higher concentrations xanthurenic acid (20 μM and 40 μM) damaged mitochondria were observed in the perinuclear region, and nuclear DNA cleavage was observed. We observed an induction of calpain Lp 82 and an increase of free Ca(2+) in the cells in a xanthurenic acid concentration-dependent manner. CONCLUSIONS: The results show that xanthurenic acid accumulation in human lens epithelial cells disturbs the normal cell physiology and leads to a cascade of pathological events. Xanthurenic acid induces calpain Lp82 and caspases in the cells growing in the dark and can be involved in senile cataract development

Software Assists in Responding to Anomalous Conditions

Fault Induced Document Retrieval Officer (FIDO) is a computer program that reduces the need for a large and costly team of engineers and/or technicians to monitor the state of a spacecraft and associated ground systems and respond to anomalies. FIDO includes artificial-intelligence components that imitate the reasoning of human experts with reference to a knowledge base of rules that represent failure modes and to a database of engineering documentation. These components act together to give an unskilled operator instantaneous expert assistance and access to information that can enable resolution of most anomalies, without the need for highly paid experts. FIDO provides a system state summary (a configurable engineering summary) and documentation for diagnosis of a potentially failing component that might have caused a given error message or anomaly. FIDO also enables high-level browsing of documentation by use of an interface indexed to the particular error message. The collection of available documents includes information on operations and associated procedures, engineering problem reports, documentation of components, and engineering drawings. FIDO also affords a capability for combining information on the state of ground systems with detailed, hierarchically-organized, hypertext- enabled documentation

CORSIA: The first internationally adopted approach to calculate life-cycle GHG emissions for aviation fuels

The aviation sector has grown at a significant pace in recent years, and despite improvements in aircraft efficiency, the sector's impact on climate change is a growing concern. To address this concern, the International Civil Aviation Organization (ICAO) established the Carbon Offsetting and Reduction Scheme for International Aviation (CORSIA) to help reduce aviation greenhouse gas (GHG) emissions. This paper presents a methodology agreed by the 193 ICAO member states to evaluate the life-cycle GHG emissions of sustainable aviation fuels (SAFs), in the CORSIA system. The core life-cycle assessment and induced land use change values of SAFs are presented to determine the GHG savings of certified pathways. The paper aims to present that a number of SAFs can yield significant life-cycle emission reductions compared to petroleum-derived jet fuel. This implies the potentially major role of SAFs in reducing aviation's carbon footprint

Social physique anxiety and physical activity in early adolescent girls : the influence of maturation and physical activity motives

This study considered the influence of maturation on social physique anxiety (SPA), the relationship between SPA and current and future physical activity (PA) levels and the influence of motives for physical activity on this relationship in early adolescent girls (n=162; mean age=11.80±0.33 years). Participants completed the Pubertal Development Scale, the modified Social Physique Anxiety Scale and the Motives for Physical Activity Scale at baseline and the Physical Activity Questionnaire for Older Children at baseline and 6 months later. The girls became less active across the 6 months and girls in the early stages of maturation had significantly lower SPA than the girls in the middle and late stages of maturation. SPA was not related to current or future physical activity in the sample as a whole. Cluster analysis identified four groups with different motive profiles and the High Appearance and Fitness group demonstrated a moderate negative relationship between SPA and PA at phase 1, whereas the other groups did not. These findings indicate that SPA may increase with maturation and the relationship between SPA and PA is dependent on reasons for being active. For girls who are motivated to be active primarily by body-related reasons SPA is likely to lead to lower levels of PA

Large-Amplitude Ultraviolet Variations in the RR Lyrae Star ROTSE-I J143753.84+345924.8

The NASA Galaxy Evolution Explorer (GALEX) satellite has obtained simultaneous near and far ultraviolet light curves of the ROTSE-I Catalog RR Lyrae ab-type variable star J143753.84+345924.8. A series of 38 GALEX Deep Imaging Survey observations well distributed in phase within the star's 0.56432d period shows an AB=4.9mag variation in the far UV (1350-1750A) band and an AB=1.8mag variation in the near UV (1750-2750A) band, compared with only a 0.8mag variation in the broad, unfiltered ROTSE-I (4500-10000A) band. These GALEX UV observations are the first to reveal a large RR Lyrae amplitude variation at wavelengths below 1800A. We compare the GALEX and ROTSE-I observations to predictions made by recent Kurucz stellar atmosphere models. We use published physical parameters for the comparable period (0.57433d), well-observed RR Lyrae star WY Antliae to compute predicted FUV, NUV, and ROTSE-I light curves for J143753.84+345924.8. The observed light curves agree with the Kurucz predictions for [Fe/H]=-1.25 to within AB=0.2mag in the GALEX NUV and ROTSE-I bands, and within 0.5mag in the FUV. At all metallicities between solar and one hundredth solar, the Kurucz models predict 6-8mag of variation at wavelengths between 1000-1700A. Other variable stars with similar temperature variations, such as Cepheids, should also have large-amplitude FUV light curves, observable during the ongoing GALEX imaging surveys.Comment: This paper will be published as part of the Galaxy Evolution Explorer (GALEX) Astrophysical Journal Letters Special Issue. Links to the full set of papers will be available at http:/www.galex.caltech.edu/PUBLICATIONS after November 22, 200