Analysis of Weighted Fraction of Length for Interfacial Gap in Cervical Composite Restorations as a Function of the Number of B-Scans of OCT Volume Scans

In dental research, the morphometric assessment of restorations is a challenge. This also applies to the assessment of the length of interfacial adhesive defects in composite restorations as a measure of tooth-restoration bond failure. The determined mean fractions of interfacial gap length on enamel and dentin interfaces deviate from the true means (N → ∞), depending on the number (Ni) of object layers assessed. Cervical composite restorations were imaged with spectral domain optical coherence tomography (SD-OCT). The mean fractions of interfacial gap length on enamel and dentin were determined for an increasing number of OCT cross-sectional images (B-scans) per restoration and were graphically displayed as a function of the number of B-scans. As the number of B-scans increased, the calculated object means approached a range of ±2.5%. This analysis is appropriate for displaying the relationship between the determined mean fraction of interfacial gap length at the enamel/dentin-restoration interface and the number of B-scans

An Intraoral OCT Probe to Enhanced Detection of Approximal Carious Lesions and Assessment of Restorations

Caries, the world’s most common chronic disease, remains a major cause of invasive restorative dental treatment. To take advantage of the diagnostic potential of optical coherence tomography (OCT) in contemporary dental prevention and treatment, an intraorally applicable spectral-domain OCT probe has been developed based on an OCT hand-held scanner equipped with a rigid 90°-optics endoscope. The probe was verified in vitro. In vivo, all tooth surfaces could be imaged with the OCT probe, except the vestibular surfaces of third molars and the proximal surface sections of molars within a 'blind spot' at a distance greater than 2.5 mm from the tooth surface. Proximal surfaces of 64 posterior teeth of four volunteers were assessed by intraoral OCT, visual-tactile inspection, bitewing radiography and fiber-optic transillumination. The agreement in detecting healthy and carious surfaces varied greatly between OCT and established methods (18.2–94.7%), whereby the established methods could always be supplemented by OCT. Direct and indirect composite and ceramic restorations with inherent imperfections and failures of the tooth-restoration bond were imaged and qualitatively evaluated. The intraoral OCT probe proved to be a powerful technological approach for the non-invasive imaging of healthy and carious hard tooth tissues and gingiva as well as tooth-colored restorations

Emissions from a modern log wood masonry heater and wood pellet boiler : Composition and biological impact on air-liquid interface exposed human lung cancer cells

The consumption of wood fuel is markedly increasing in developing and industrialized countries. Known side effects of wood smoke inhalation manifest in proinflammatory signaling, oxidative stress, DNA damage and hence increased cancer risk. In this study, the composition and acute biological impact of emissions of state-of-the-art wood combustion compliances: masonry heater (MH) and pellet boiler (PB) were investigated. Therefore A549 cells were exposed to emission aerosols in an automated air-liquid interface exposure station followed by cytotoxicity, transcriptome and proteome analyses. In parallel, aerosols were subjected to a chemical and physical haracterization. Compared to PB, the MH combustion at the same dilution ratio resulted in a 3-fold higher particle mass concentration (PM2.5) and deposited dose (PB: 27 $\pm$ 2 ng/cm2, MH; 73 $\pm$ 12 ng/cm2). Additionally, the MH aerosol displayed a substantially larger concentration of aldehydes, polycyclic aromatic hydrocarbons (PAH) or oxidized PAH. Gene ontology analysis of transcriptome of A549 cells exposed to MH emissions revealed the activation of proinflammatory response and key signaling cascades MAP kinase and JAK-STAT. Furthermore, CYP1A1, an essential enzyme in PAH metabolism, was induced. PB combustion aerosol activated the proinflammatory marker IL6 and different transport processes. The proteomics data uncovered induction of DNA damage-associated proteins in response to PB and DNA doublestrand break processing proteins in response to MH emissions. Taking together, the MH produces emissions with a higher particle dose and more toxic compounds while causing only mild biological responses. This finding points to a significant mitigating effect of antioxidative compounds in MH wood smoke

TRY plant trait database – enhanced coverage and open access

Plant traits - the morphological, anatomical, physiological, biochemical and phenological characteristics of plants - determine how plants respond to environmental factors, affect other trophic levels, and influence ecosystem properties and their benefits and detriments to people. Plant trait data thus represent the basis for a vast area of research spanning from evolutionary biology, community and functional ecology, to biodiversity conservation, ecosystem and landscape management, restoration, biogeography and earth system modelling. Since its foundation in 2007, the TRY database of plant traits has grown continuously. It now provides unprecedented data coverage under an open access data policy and is the main plant trait database used by the research community worldwide. Increasingly, the TRY database also supports new frontiers of trait‐based plant research, including the identification of data gaps and the subsequent mobilization or measurement of new data. To support this development, in this article we evaluate the extent of the trait data compiled in TRY and analyse emerging patterns of data coverage and representativeness. Best species coverage is achieved for categorical traits - almost complete coverage for ‘plant growth form’. However, most traits relevant for ecology and vegetation modelling are characterized by continuous intraspecific variation and trait–environmental relationships. These traits have to be measured on individual plants in their respective environment. Despite unprecedented data coverage, we observe a humbling lack of completeness and representativeness of these continuous traits in many aspects. We, therefore, conclude that reducing data gaps and biases in the TRY database remains a key challenge and requires a coordinated approach to data mobilization and trait measurements. This can only be achieved in collaboration with other initiatives

Analysis of Weighted Fraction of Length for Interfacial Gap in Cervical Composite Restorations as a Function of the Number of B-Scans of OCT Volume Scans

In dental research, the morphometric assessment of restorations is a challenge. This also applies to the assessment of the length of interfacial adhesive defects in composite restorations as a measure of tooth-restoration bond failure. The determined mean fractions of interfacial gap length on enamel and dentin interfaces deviate from the true means (N → ∞), depending on the number (Ni) of object layers assessed. Cervical composite restorations were imaged with spectral domain optical coherence tomography (SD-OCT). The mean fractions of interfacial gap length on enamel and dentin were determined for an increasing number of OCT cross-sectional images (B-scans) per restoration and were graphically displayed as a function of the number of B-scans. As the number of B-scans increased, the calculated object means approached a range of ±2.5%. This analysis is appropriate for displaying the relationship between the determined mean fraction of interfacial gap length at the enamel/dentin-restoration interface and the number of B-scans

Analysis of Weighted Fraction of Length for Interfacial Gap in Cervical Composite Restorations as a Function of the Number of B-Scans of OCT Volume Scans

In dental research, the morphometric assessment of restorations is a challenge. This also applies to the assessment of the length of interfacial adhesive defects in composite restorations as a measure of tooth-restoration bond failure. The determined mean fractions of interfacial gap length on enamel and dentin interfaces deviate from the true means (N → ∞), depending on the number (Ni) of object layers assessed. Cervical composite restorations were imaged with spectral domain optical coherence tomography (SD-OCT). The mean fractions of interfacial gap length on enamel and dentin were determined for an increasing number of OCT cross-sectional images (B-scans) per restoration and were graphically displayed as a function of the number of B-scans. As the number of B-scans increased, the calculated object means approached a range of ±2.5%. This analysis is appropriate for displaying the relationship between the determined mean fraction of interfacial gap length at the enamel/dentin-restoration interface and the number of B-scans

Analysis of Weighted Fraction of Length for Interfacial Gap in Cervical Composite Restorations as a Function of the Number of B-Scans of OCT Volume Scans

In dental research, the morphometric assessment of restorations is a challenge. This also applies to the assessment of the length of interfacial adhesive defects in composite restorations as a measure of tooth-restoration bond failure. The determined mean fractions of interfacial gap length on enamel and dentin interfaces deviate from the true means (N → ∞), depending on the number (Ni) of object layers assessed. Cervical composite restorations were imaged with spectral domain optical coherence tomography (SD-OCT). The mean fractions of interfacial gap length on enamel and dentin were determined for an increasing number of OCT cross-sectional images (B-scans) per restoration and were graphically displayed as a function of the number of B-scans. As the number of B-scans increased, the calculated object means approached a range of ±2.5%. This analysis is appropriate for displaying the relationship between the determined mean fraction of interfacial gap length at the enamel/dentin-restoration interface and the number of B-scans

Dataset on FAP-induced emergence of spontaneous metastases and on the preparation of activatable FAP-targeting immunoliposomes to detect the metastases

The underlying data demonstrates that fibroblast activation protein (FAP) paves the way for fibrosarcoma cells, which require the proteolysis of the extracellular matrix (ECM) and basement membranes to intravasate from implanted subcutaneous primary tumors into blood vessels, be transported to distant organs where they extravasate from the blood vessels, reattach and proliferate to metastases. The data additionally shows that FAP, when overexpressed on fibrosarcoma cells induces their invasion and formation of spontaneous metastases in multiple organs, particularly after subcutaneous co-implantation of the FAP-expressing and wildtype fibrosarcoma. The raw and processed data presented herein is related to a research article entitled “Potential of activatable FAP-targeting immunoliposomes in intraoperative imaging of spontaneous metastases” (F.L. Tansi, R. Rüger, C. Böhm, R.E. Kontermann, U.K. Teichgraeber, A. Fahr, I. Hilger, 2016) [1]. Furthermore, evidence for the detection of FAP-expressing tumor cells and cells of the tumor stroma by activatable FAP-targeting liposomes is presented in this dataset

Visualization of interfacial adhesive defects at dental restorations with spectral domain and polarization sensitive optical coherence tomography

Restoration loss based on interfacial adhesive defects and associated caries at the restoration margin are the main causes for invasive replacement of dental restorations. Assessment of the interfacial quality based on clinical inspection and radiographic examination is often difficult and not reliable. In this work, we present spectral domain optical coherence tomography (SDOCT) and polarization sensitive optical coherence tomography (PSOCT) for the evaluation of tooth-composite bond failure. Imaging of two composite restorations at the occlusal surface are presented using intensity-based images obtained by SDOCT and PSOCT based degree of polarization uniformity (DOPU). Both modalities revealed several defects beneath the surface such as inhomogeneous adhesive layers, marginal gaps and bubbles. In addition, DOPU representation showed an inhomogeneous structure within the composite material. OCT based imaging of dental restorations could add a valuable diagnostic tool for the evaluation of structural defects in clinical practice. The representation of polarization characteristics with the DOPU algorithm provides further information on the homogeneity of the restoration

An Intraoral OCT Probe to Enhanced Detection of Approximal Carious Lesions and Assessment of Restorations

Caries, the world’s most common chronic disease, remains a major cause of invasive restorative dental treatment. To take advantage of the diagnostic potential of optical coherence tomography (OCT) in contemporary dental prevention and treatment, an intraorally applicable spectral-domain OCT probe has been developed based on an OCT hand-held scanner equipped with a rigid 90°-optics endoscope. The probe was verified in vitro. In vivo, all tooth surfaces could be imaged with the OCT probe, except the vestibular surfaces of third molars and the proximal surface sections of molars within a 'blind spot' at a distance greater than 2.5 mm from the tooth surface. Proximal surfaces of 64 posterior teeth of four volunteers were assessed by intraoral OCT, visual-tactile inspection, bitewing radiography and fiber-optic transillumination. The agreement in detecting healthy and carious surfaces varied greatly between OCT and established methods (18.2–94.7%), whereby the established methods could always be supplemented by OCT. Direct and indirect composite and ceramic restorations with inherent imperfections and failures of the tooth-restoration bond were imaged and qualitatively evaluated. The intraoral OCT probe proved to be a powerful technological approach for the non-invasive imaging of healthy and carious hard tooth tissues and gingiva as well as tooth-colored restorations