A calm companion lowers fear in groups of dairy cows

Dairy cows are generally calm and compliant, but some management procedures can make cows fearful or stressed. Not only are fearful cattle a threat to human safety, but fear is also detrimental to animal welfare and productivity. This study aimed to test whether fear in small groups of dairy cattle could be attenuated by the presence of a calm and experienced companion. Twenty-seven dairy cows from a Swedish agricultural school participated in the study. The study included a standardized fear-eliciting stimulus, which was 3 sudden, repetitive openings of a red and white umbrella. Demonstrator cows (n = 9) were selected based on age to ensure that all demonstrators were older than the naïve test cows (n = 18). Of these 9 demonstrator cows, 6 were selected as untrained (i.e., habituated to the presence of the test person) and 3 were selected as trained demonstrators (i.e., additionally habituated to the fear-eliciting stimulus). The remaining 18 test cows comprised 6 test-cow groups of 3 cows each, which were their own controls, resulting in a crossover design; 3 groups were tested with a trained demonstrator first and then with an untrained demonstrator, and vice versa for the other 3 groups, resulting in a total of 12 trials (4 sub-treatments). Response variables were heart rate increase from baseline, behavioral reaction indicative of fear, and latency to resume feeding after exposure to the fear-eliciting stimulus. The study found a calming effect of a trained demonstrator on test cows' heart rate but not on latency to resume feeding or behavioral reaction. Post hoc analyses revealed a carryover effect on latency, indicating that test cows who were accompanied by an untrained demonstrator first had longer latencies than cows in all other sub-treatments. Adding a calm, experienced cow to groups of dairy cattle may mitigate fear and thereby improve welfare and safety

Opportunities (and challenges) in dairy cattle cognition research: A key area needed to design future high welfare housing systems

The ability of dairy cattle to adapt to husbandry systems and management routines is crucial for ensuring higher welfare and efficient production. However, this ability can be compromised by our limited knowledge of their cognitive abilities, which may result in suboptimal husbandry and management standards. In this narrative review, we highlight three topics of cattle cognition research that are currently understudied, and yet key to developing future high welfare dairy cattle housing systems: 1) transmission of information from cow to calf, 2) mechanisms to attenuate fear, and 3) cognitive processes involved in the human-cattle relationship. We review the currently available literature on all three topics and highlight promising research areas from an animal husbandry point of view. We conclude that future studies should focus on elucidating what, and how much, calves learn from their dam during prolonged cow-calf contact in dairy cattle systems. Such information could constitute an important part of the discussion of whether to keep cows and calves together for a longer time after calving in the dairy industry. Fear in the cattle group might be lowered by the use of calm companions and future studies could uncover if attenuation of fear might even be induced by conditioning positive experiences of cattle with unrelated stimuli such as odours. Lastly, the human-cattle relationship might benefit from utilising the already established training regimes from other species, for example positive reinforcement training or target training, which may have the potential to decrease risk of injury during handling for both the cow and the handler

Horse odor exploration behavior is influenced by pregnancy and age

In spite of a highly developed olfactory apparatus of horses, implying a high adaptive value, research on equine olfaction is sparse. Our limited knowledge on equine olfaction poses a risk that horse behavior does not match human expectations, as horses might react fearful when exposed to certain odors, which humans do not consider as frightening. The benefit of acquiring more knowledge of equine olfaction is therefore twofold; (1) it can aid the understanding of horse behavior and hence reduce the risk of dangerous situations, and (2) there may be unexplored potential of using odors in several practical situations where humans interact with horses. This study investigated behavior and olfactory sensitivity of 35 Icelandic horses who were presented with four odors: peppermint, orange, lavender and cedar wood in a Habituation/Dishabituation paradigm. The response variables were sniffing duration per presentation and behavioral reaction (licking, biting, snorting, and backing), and data were analyzed for potential effects of age, sex and pregnancy. Results showed that habituation occurred between successive odor presentations (1st vs. 2nd and 2nd vs. 3rd presentations: P P P P = 0.0068). Pregnant mares sniffed odors less than non-pregnant mares (P = 0.030), young horses (age 0-5 years) sniffed cedar wood for longer than old horses (P = 0.030), whereas sex had no effect (P > 0.050). The results show that horses’ odor exploration behavior and interest in odors varies with age and pregnancy and that horses naïve to the taste of a substrate, may be able to link smell with taste, which has not been described before. These results can aid our understanding of horses’ behavioral reactions to odors, and in the future, it may be possible to relate these to the physiology and health of horses

Dairy cows did not rely on social learning mechanisms when solving a spatial detour task

As herd-living animals, cattle have opportunities to observe and learn from others. While there is evidence of simpler processes of information transfer in cattle (social facilitation and stimulus enhancement), true social learning mechanisms in cattle remain largely unexplored. This study aimed to investigate if dairy cows possess cognitive abilities to acquire new behavior through social learning in a spatial detour task. Thirty-two dairy cows (ages 2-9 years) participated in the study. A food reward was placed behind a U-shaped formation (4x2 m), allowing the cows to see but not reach the reward without first detouring around the obstacle. The U-shape provided two routes (approx. 18 m walking distance) to the reward, of which one was used for demonstration. Two cows were demonstrators and 30 cows were divided into two groups, assigned as either observers of demonstration (n=15) or controls not observing demonstration (n=15). Cows had three attempts (trials) to solve the task. Response variables were: success, latency to reach the reward, concordance in choice of route to detour, and time spent facing the test arena before each trial started. The study found no significant differences in success or latency between observers and controls, although observers spent a greater proportion of the time before trials facing the test arena. However, successful observers tended to be faster than successful controls. Individual cows were generally consistent in their choice of route, and cows choosing the demonstrated route were significantly faster than cows that did not. Success in solving the task decreased over trials, likely due to decreasing food motivation. Age had a significant effect on success in 2nd and 3rd trials, with younger cows being more successful. The lacking effect of treatment on success suggests that the age effect may be explained by a higher motivation, rather than social learning. Adding to the sparse knowledge of social learning in farm animals, these results indicate that cows did not utilize social learning mechanisms when solving the detour task. Future research should focus on clarifying whether cattle possess cognitive abilities necessary for social learning, as well as if /when social learning is a primary strategy

Horse odor exploration behavior is influenced by pregnancy and age

In spite of a highly developed olfactory apparatus of horses, implying a high adaptive value, research on equine olfaction is sparse. Our limited knowledge on equine olfaction poses a risk that horse behavior does not match human expectations, as horses might react fearful when exposed to certain odors, which humans do not consider as frightening. The benefit of acquiring more knowledge of equine olfaction is therefore twofold; (1) it can aid the understanding of horse behavior and hence reduce the risk of dangerous situations, and (2) there may be unexplored potential of using odors in several practical situations where humans interact with horses. This study investigated behavior and olfactory sensitivity of 35 Icelandic horses who were presented with four odors: peppermint, orange, lavender and cedar wood in a Habituation/Dishabituation paradigm. The response variables were sniffing duration per presentation and behavioral reaction (licking, biting, snorting, and backing), and data were analyzed for potential effects of age, sex and pregnancy. Results showed that habituation occurred between successive odor presentations (1st vs. 2nd and 2nd vs. 3rd presentations: P < 0.001), and dishabituation occurred when a new odor was presented (1st vs. 3rd presentations: P < 0.001). Horses were thus able to detect and distinguish between all four odors, but expressed significantly longer sniffing duration when exposed to peppermint (peppermint vs. orange, lavender and cedar wood: P < 0.001). More horses expressed licking when presented to peppermint compared to cedar wood and lavender (P = 0.0068). Pregnant mares sniffed odors less than non-pregnant mares (P = 0.030), young horses (age 0-5 years) sniffed cedar wood for longer than old horses (P = 0.030), whereas sex had no effect (P > 0.050). The results show that horses’ odor exploration behavior and interest in odors varies with age and pregnancy and that horses naïve to the taste of a substrate, may be able to link smell with taste, which has not been described before. These results can aid our understanding of horses’ behavioral reactions to odors, and in the future, it may be possible to relate these to the physiology and health of horses

Social Learning in Horses—Fact or Fiction?

Prima facie, the acquisition of novel behaviors in animals through observation of conspecifics seems straightforward. There are, however, various mechanisms through which the behavior of animals can be altered from observing others. These mechanisms range from simple hard-wired contagious processes to genuine learning by observation, which differ fundamentally in cognitive complexity. They range from social facilitation and local enhancement to true social learning. The different learning mechanisms are the subject of this review, largely because research on learning by observation can be confounded by difficulties in interpretation owing to the looming possibility of associative learning infecting experimental results. While it is often assumed that horses are capable of acquiring new behavior through intra-species observation, research on social learning in horses includes a variety of studies some of which may overestimate the possession of higher mental abilities. Assuming such abilities in their absence can have welfare implications, e.g., isolating stereotypical horses on the assumption that these behaviors can be learned though observation by neighboring horses. This review summarizes the definitions and criteria for the various types of social transmission and social learning and reviews the current documentation for each type in horses with the aim of clarifying whether horses possess the ability to learn through true social learning. As social ungulates, horses evolved in open landscapes, exposed to predators and grazing most of the day. Being in close proximity to conspecifics may theoretically offer an opportunity to learn socially, however anti-predator vigilance and locating forage may not require the neural complexity of social learning. Given the significant energetic expense of brain tissue, it is likely that social facilitation and local enhancement may have been sufficient in the adaptation of equids to their niche. As a consequence, social learning abilities may be maladaptive in horses. Collectively, the review proposes a novel differentiation between social transmission (social facilitation, local, and stimulus enhancement) and social learning (goal emulation, imitation). Horses are undoubtedly sensitive to intra-species transfer of information but this transfer does not appear to satisfy the criteria for social learning, and thus there is no solid evidence for true social learning in horses

Rider effects on horses’ conflict behaviour, rein tension, physiological measures and rideability scores

Many breeding organisations include a subjective scoring of rideability by a professional rider into their evaluation of sports horses, but the consistency and reliability of the scoring system is debateable. The aim of this study was to investigate (i) whether professional riders agree in their scoring of rideability, and (ii) whether rideability scores are affected by rein tension, horse conflict behaviour, heart rate, and salivary cortisol, and (iii) whether riders induce different levels of conflict behaviour and physiological responses in the horses. Ten professional, female riders each rode 10 dressage horses (level M German scale; n = 100 combinations) through a standardised dressage test (10 min warm-up followed by a 4-min test) and subsequently scored the horses for rideability on the official 1–10 scale (1 = poor to 10 = excellent) from the Danish Riding Federation. Rein tension, horse heart rate, saliva cortisol and conflict behaviour were measured for each rider-horse pair. The riders were inconsistent in their scoring of rideability to the individual horses, e.g. scores for one of the horses ranged from 1 to 8. There was a significant effect of rider (P = 0.003) and the frequency of conflict behaviour (undesired head movements: P < 0.001, breaking the gait: P = 0.013, and other evasive behaviour: P = 0.032) on rideability scores, i.e. the more conflict behaviour the lower the score. There was no significant effect of rein tension and the physiological measures on rideability scores. However, there was a significant effect of rider on rein tension, horses’ heart rate and increases in saliva cortisol concentrations and a tendency for some types of conflict behaviour, suggesting that some riders induced more discomfort in the horses. Future studies could help shed light on which elements of riding style are particularly important for sports horse welfare. In conclusion, this study found a large variation in rideability scores assigned to ten sports horses by ten professional riders. Rideability scores were dependent on the level of horse conflict behaviour, but not rein tension and physiological measures. Further studies are needed to improve the objectivity, consistency and reliability of rideability assessment of sports horses

Odor exploration behavior of the domestic pig (Sus scrofa) as indicator of enriching properties of odors

Introduction and aim: Although the sense of smell in pigs is widely recognized as being highly developed, surprisingly little is known about their sensory ability. This study aimed to (a) identify which non-social odors pigs were able to detect and distinguish between, (b) investigate the types of behavior expressed when exploring odors and, (c) compare pigs’ responses to the different odors to evaluate their interest in the odors.Methods: Growing pigs (N = 192) of crossbred commercial breeds were enrolled in the experiment (32–110 days of age, weighing 64.9 ± 10.1kg). Littermate pairs of opposite sex were tested in test pens with two odor insertion points in the pen wall, 55 cm apart. All pigs were habituated to the test pens and experimenters. Twelve odors were tested (eight essential oils and four synthetic perfumes) in groups of three odors, with each pig pair tested once with one set of three odors (all possible orders of the three odors were tested on 24 pairs in total), always against a non-odor control (demineralized water). In a test, each of the three odors were presented during three trials in a row (a total of 9 trials per test; trial duration: 1 min; inter-trial breaks: 2 min; total test duration: 25 min). Response variables included: duration of sniffing, feeding-related behavior (licking, biting and rooting), agonistic behavior (biting, displacement and pushing) and no approach of the odor or control, recorded throughout each 1-min odor presentation.Results: All pigs sniffed an odor less when repeatedly presented (LMM: all odors P Conclusion: Collectively, pigs express sniffing, agonistic, and feeding-related behavior when exploring odors, which suggests that pigs perceive odors of non-social origin as a resource. Odors may thus constitute relevant enrichment material for pigs

A systematic review of the effects of prolonged cow-calf contact on behavior, welfare, and productivity

Separation of calves from cows within hours or days of birth is common on dairy farms. Stakeholders have conflicting perspectives on whether this is harmful or beneficial for the animals’ welfare and production. Our objective was to critically evaluate the scientific evidence for both acute and long-term effects of early separation versus an extended period of cow-calf contact. The outcomes investigated were the behavior, welfare (excluding physical health) and performance (milk yield and growth, respectively) of dairy cows and calves. Primary research papers were found through targeted Web of Science searches, the reference lists of recent reviews for each topic, and the reference lists of papers identified from these sources. Studies were included if they were published in English, the full text was accessible, and they compared treatments with and without contact between dairy cows and calves for a specified period. Early separation (within 24 h post-partum) was found to reduce acute distress responses of cows and calves. However, longer cow-calf contact typically had positive longer-term effects on calves, promoting more normal social behavior, reducing abnormal behavior and sometimes reducing responses to stressors. In terms of productivity, allowing cows to nurse calves generally decreased the volume of milk available for sale during the nursing period, but there was no consistent evidence of reduced milk production over a longer period. Allowing a prolonged period of nursing increased calf weight gains during the milk-feeding period. In summary, extended cow-calf contact aggravates the acute distress responses and reduces the amount of saleable milk while the calves are suckling, but can have positive effects on behaviors relevant to welfare in the longer term and benefit calf growth. The strength of these conclusions is limited, however, given that relatively few studies address most of these effects and that experimental design including timing of contact and observations are often inconsistent across studies. Few studies presented indicators of long-term welfare effects other than abnormal and social behavior of the calves