Value of Indirect Hemagglutination and Coagglutination Tests for Serotyping Haemophilus parasuis

P. 880-882An indirect hemagglutination test (IHA) and a coagglutination test (CA) were evaluated using saline, boiled, and autoclaved extracts for serotyping Haemophilus parasuis. CA showed several cross-reactions, whereas IHA gave rise to specific reactions, with minor exceptions. IHA was further compared with the immunodiffusion test (the “gold standard”) for the serotyping of 67 field isolates. As a conclusion, IHA is recommended as a useful method for sensitive and specific serotyping of H. parasuisS

Identification and characterization of the TonB region and its role in transferrin-mediated iron acquisition in Haemophilus parasuis

12 p.Haemophilus parasuis is the causative agent of Glässer's disease, which is responsible for considerable economic losses in the pigrearing industry. The aim of the study reported here was the identification, sequencing and molecular characterization of the TonB region that includes tonB, exbBD, and tbpBA genes in H. parasuis. In addition, two fusion proteins were generated. One of them (pGEX-6P-1-GST-TbpB) contained the first 501 amino acids of H. parasuis TbpB protein, while the second (pBAD-Thio-TbpBV5- His) included the first 102 amino acids of H. parasuis TbpB N-terminus domain. A panel of 14 hybridomas secreting monoclonal antibodies was raised against the two recombinant TbpB fusion proteins. Furthermore, to assess whether the expression of the H. parasuis ExbB, TbpB, and TbpA proteins was upregulated under conditions of restricted availability of iron, a rabbit polyclonal antibody against H. parasuis TbpB-His fusion protein was produced. A rabbit polyclonal antibody against serotype 7 of Actinobacillus pleuropneumoniae ExbB and TbpA proteins was also used for the detection of the homologous proteins in H. parasuis. Overall, the data indicate that H. parasuis, like other members of the Pasteurellaceae family, possesses the genetic elements of the TonB region for iron acquisition and the transferrin-binding proteins encoded under this region are upregulated under restricted iron availabilityS

Molecular characterization of Haemophilus parasuis ferric hydroxamate uptake (fhu) genes and constitutive expression of the FhuA receptor

P. 49-59Bacteria have evolved a set of highly specialized proteins to capture iron in irondepleted environments. The acquisition and uptake of iron present in the extracellular milieu of eukaryotic organisms is indispensable for the growth and survival of microbial pathogens in the course of infection. Haemophilus parasuis is the causative agent of Glässer disease, which is responsible for considerable financial losses in pig-rearing worldwide. To gain insight into the mechanisms involved in siderophore-mediated iron uptake in H. parasuis, genes in the H. parasuis ferric hydroxamate uptake (Fhu) region were amplified in the work being reported here. As has been described in A. pleuropneumoniae, an Fhu genomic region was also present in H. parasuis, being composed of four potential consecutive open reading frames (ORF) designated as fhuC, fhuD, fhuB, and fhuA, respectively. By immunoblotting, using a cross-reactive polyclonal antibody raised against Actinobacillus pleuropneumoniae FhuA protein, it was demonstrated that this protein was constitutively expressed in H. parasuis and its level of expression was not modified under conditions of restricted iron availability. This is the first report describing the presence of the fhu genes in H. parasuis. Our results indicate that FhuA protein expression is not affected under ironrestricted conditions, however, it is one of the targets of the humoral immune responseS

Understanding systemic land use dynamics in conflict-affected territories: The cases of Cesar and Caquetá, Colombia

In the Colombian context, disputes over natural resources, mainly over land, and poor governance are intertwined with armed conflict. Although efforts to address this situation, including the 2016 peace agreement signed between Colombian government and the Revolutionary Armed Forces of Colombia (FARC, by Spanish acronym) are underway, these disputes continue, affecting land use dynamics. Understanding the complexity and trends in land use conflicts, as well as the specific regional characteristics underlying differing land use changes across regions, is critical. This article aims to systematically understand land use dynamics in two contrasting and conflict-affected territories in Colombia, Caquetá and Cesar, thus identifying entry points to address land-use conflicts at the regional level. To address the complexity of each regional case, we apply a methodology based on system thinking to capture the interconnections between socio-economic and environmental system components and their land use dynamics. Results depicted through causal loop diagrams not just show the cascade of environmental, social, and economic failures resulting from land use changes in these two conflict-affected territories but also suggest that land tenure systems innovations and the promotion of sustainable land use interventions at the regional level can reverse the consequences of the land use changes. Thus, future actions addressing land use conflicts must be context-dependent, tackling the root and structural causes

Rapid Intensity Decrease During the Second Half of the First Millennium BCE in Central Asia and Global Implications

Recent paleomagnetic studies have shown that important short-lived intensity fluctuations occurred during the first millennium BCE. However, the knowledge of the spatial and temporal extension of these features is still limited by the scarce availability of robust data. In this study we focus on the study of the intensity decrease that took place in Central Asia during the second half of the 1st millennium BCE after the high intensities that characterized the Levantine Iron Age Anomaly. Since previous archeointensities available for this period and region were obtained without accomplishing modern standards of quality, we present here new archeointensities that are derived from classical Thellier and Thellier experiments, including partial thermoremanent magnetization (pTRM) checks, thermoremanent magnetization (TRM) anisotropy and cooling rate corrections at the specimen level. The new 51 archeointensities, together with previous archeointensities, have been used to present a new local paleosecular variation curve for Central Asia. The results confirm the existence of an important geomagnetic field intensity decrease in South Uzbekistan from the 4th century BCE to the end of the 1st century BCE associated with rates of changes up to −15 μT/century. A critical analysis of the archeointensity global database indicates that this feature was present at continental scale, from Western Europe to Central Asia. However, this trend is not identified in other regions such as Japan or Mexico. Finally, the comparison with the dipole moment derived from recent global geomagnetic field reconstructions suggests a strong influence of non-dipolar sources upon this continental intensity feature

Fixed and drifting buoys around the national Spanish waters

Joint Technical Commission for Oceanography and Marine Meteorology (JCOMM) Marine Technical Conference, The Technical Conference (TECO) Toward an Integrated Met-ocean Monitoring, Forecasting and Services System, 25-29 October 2017, Geneva, SwitzerlandImproving the knowledge of the ocean and seas surrounding the Iberian Peninsula and Balearic and Canary islands is an objective of the Spanish oceanography. For that purpose, a number of fixed and drifting floats have been established in the last 25 years. Data buoys measure sea surface temperature and salinity, ocean current velocity, air temperature, humidity, wave characteristic and wind velocity across seas and ocean. The objective is increase the quantity, quality, coverage and timeliness of atmospheric and oceanographic data. These observations are used immediately to improve forecast and therefore increase marine safety. The main group of fixed buoys is formed by the Puertos del Estado deep and shallow buoy networks, but a series of well instrumented new platforms has been established in later times. The RAIA Project (Xunta de Galicia), PLOCAN, SOCIB, IEO, Euskalmet-AZTI, ICM and UTM (CSIC) and University and Polytechnic of Barcelona have completed the Observing System. Most of the buoys are transmitting data by GTS for using in atmospheric and ocean prediction models. Multidisciplinary sensors as Dissolved Oxygen, Fluorescence Chlorophyll or pCO2 has been mounted in the buoys and calibration/validation procedures has been developed for improve data quality. Antifouling systems recently developed have also been included and quality of the optical sensors measurements has improved. Drifting floats has increase its number and importance, from Argo floats to traditional deriving ones improving the Spanish contribution to IOC and WWO and JCOMM. Spain is member of EuroArgo ERIC. SOCIB and IEO are the main contributors. Also multidisciplinary work has been done associated to Argo buoys. BGQ ARGO incorporate O2 sensor. ICM, SMOS Barcelona Expert Center, and SOCIB are the main contributors to the drifting buoys group. Main objectives are improving Technological development as well as data management. Tropical and Southern Atlantic Ocean are the main studying areasPeer Reviewe

CGRP en migraña: de la fisiopatología a la terapéutica

Introducción En los últimos años se ha observado que moléculas como el péptido relacionado con el gen de la calcitonina (CGRP) y, en menor grado, el péptido activador de la adenilato-ciclasa pituitaria estaban elevadas durante los ataques de migraña y en la migraña crónica tanto en líquido cefalorraquídeo como en suero y que su reducción farmacológica tenía una significación clínica con una mejoría en la migraña de los pacientes. Es lógico por tanto que una de las principales líneas de investigación en migraña se base en el papel del CGRP en la fisiopatología de esta entidad. Desarrollo Desde el Grupo de Estudio de Cefaleas de la Sociedad Española de Neurología nos planteamos la redacción de este documento, cuyo objetivo es abordar, basándonos en la evidencia publicada, cuestiones tan importantes como el papel del CGRP en la fisiopatología de la migraña, el mecanismo de acción de los anticuerpos monoclonales y de los gepantes, el análisis crítico de los resultados de los diferentes estudios, el perfil del paciente que podría ser candidato al tratamiento con anticuerpos monoclonales y su impacto en términos de farmacoeconomía. Conclusiones El desarrollo clínico de los gepantes, antagonistas del CGRP, para el tratamiento agudo del ataque de migraña y de los anticuerpos monoclonales contra ligando y contra el receptor del CGRP ofrecen resultados esperanzadores para nuestros pacientes. Introduction It has been observed in recent years that levels of such molecules as calcitonin gene–related peptide (CGRP) and, to a lesser extent, the pituitary adenylate cyclase–activating peptide are elevated during migraine attacks and in chronic migraine, both in the cerebrospinal fluid and in the serum. Pharmacological reduction of these proteins is clinically significant, with an improvement in patients’ migraines. It therefore seems logical that one of the main lines of migraine research should be based on the role of CGRP in the pathophysiology of this entity. Development The Spanish Society of Neurology''s Headache Study Group decided to draft this document in order to address the evidence on such important issues as the role of CGRP in the pathophysiology of migraine and the mechanism of action of monoclonal antibodies and gepants; and to critically analyse the results of different studies and the profile of patients eligible for treatment with monoclonal antibodies, and the impact in terms of pharmacoeconomics. Conclusions The clinical development of gepants, which are CGRP antagonists, for the acute treatment of migraine attacks, and CGRP ligand and receptor monoclonal antibodies offer promising results for these patients

Fixed and Drifting Buoys around the National Spanish Waters

Improving the knowledge of the seas surrounding the Iberian Peninsula, Balearic and Canary islands is one of the objectives for the Spanish oceanographic community. For that purpose, a number of fixed and drifting buoys have been deployed in the last 25 years. Parameters measured included sea surface temperature and salinity, ocean current velocity, air temperature, humidity, wave characteristic and wind velocity. The national aim is to increase the quantity, quality, coverage and timeliness of atmospheric and oceanographic data. These observations are used immediately to improve forecast and therefore increase marine safety

Deep-sequencing reveals broad subtype-specific HCV resistance mutations associated with treatment failure

A percentage of hepatitis C virus (HCV)-infected patients fail direct acting antiviral (DAA)-based treatment regimens, often because of drug resistance-associated substitutions (RAS). The aim of this study was to characterize the resistance profile of a large cohort of patients failing DAA-based treatments, and investigate the relationship between HCV subtype and failure, as an aid to optimizing management of these patients. A new, standardized HCV-RAS testing protocol based on deep sequencing was designed and applied to 220 previously subtyped samples from patients failing DAA treatment, collected in 39 Spanish hospitals. The majority had received DAA-based interferon (IFN) a-free regimens; 79% had failed sofosbuvir-containing therapy. Genomic regions encoding the nonstructural protein (NS) 3, NS5A, and NS5B (DAA target regions) were analyzed using subtype-specific primers. Viral subtype distribution was as follows: genotype (G) 1, 62.7%; G3a, 21.4%; G4d, 12.3%; G2, 1.8%; and mixed infections 1.8%. Overall, 88.6% of patients carried at least 1 RAS, and 19% carried RAS at frequencies below 20% in the mutant spectrum. There were no differences in RAS selection between treatments with and without ribavirin. Regardless of the treatment received, each HCV subtype showed specific types of RAS. Of note, no RAS were detected in the target proteins of 18.6% of patients failing treatment, and 30.4% of patients had RAS in proteins that were not targets of the inhibitors they received. HCV patients failing DAA therapy showed a high diversity of RAS. Ribavirin use did not influence the type or number of RAS at failure. The subtype-specific pattern of RAS emergence underscores the importance of accurate HCV subtyping. The frequency of “extra-target” RAS suggests the need for RAS screening in all three DAA target regions