Análise da eficácia das Células mesenquimais como adjuvante em xeno enxertos ósseos

Introduçao: A engenheria óssea regenerativa é uma técnica utilizada com o fim de restaurar as funções estéticas e funcionais ótimas, principalmente em casos de agressões extensas ao volume ósseo. O sucesso desse procedimento depende da presença de processos osteogênicos e precursores vasculares nos tecidos circundantes, permitindo uma correta osseointegração. Uma terapia alternativa viável para a regeneração óssea é a associação de células estaminais mesenquimais (MSC) com xenoenxertos. Objetivo: Descrever e avaliar a eficácia da utilização de xenoenxertos com celulas estamimais mesenquimais como adjuvante nas terapias de engenharia regenerativa óssea guiada. Metodología: Realizou-se uma pesquisa bibliográfica na plataforma PubMed mediante um algoritmo de busca “booleano” com as seguintes palavras-chave: periodontal xenograft, stem cell, adult stem cell, guided tissue regeneration, periodontal regeneration, xenograft jaw bone, periodontal xenograft. Critérios de inclusão e exclusão foram definidos. Resultados: 21 artigos foron selecionados, após a aplicação dos critérios de elegibilidade, 13 estudos foram incluídos. Conclusões: As células mesenquimais têm potencial promissor para melhorar a eficácia dos xenoenxertos ósseos, demonstrando resultados positivos na regeneração óssea. Estudos clínicos confirmaram sua segurança e apontaram vantagens das células mesenquimais da crista ilíaca em relação a outras fontes. No entanto, são necessárias mais pesquisas para otimizar sua efetividade, considerando fatores como a escolha da fonte celular, métodos de isolamento e administração. Estudos clínicos adicionais são necessários para avaliar sua eficácia e segurança em diferentes contextos clínicos.Introduction: Regenerative bone engineering is a technique used in order to restore optimal aesthetic and functional functions, especially in cases of extensive aggression to bone volume. The success of this procedure depends on the presence of osteogenic processes and vascular precursors in the surrounding tissues, allowing for correct osseointegration. A viable alternative therapy for bone regeneration is the association of mesenchymal stem cells (MSC) with xenografts. Objective: To describe and evaluate the efficacy of the use of xenografts with mesenchymal stem cells as an adjuvant in guided bone regenerative engineering therapies. Methodology: A literature search was performed on the PubMed platform using a "Boolean" search algorithm with the following keywords: periodontal xenograft, stem cell, adult stem cell, guided tissue regeneration, Periodontal regeneration, jawbone xenograft, periodontal xenograft. Inclusion and exclusion criteria were defined. Results: 21 articles were selected, after applying the eligibility criteria, 13 studies were included. Conclusions: Mesenchymal cells have a promising potential to improve the efficacy of bone xenografts, showing positive results in bone regeneration. Clinical studies have confirmed their safety and pointed out advantages of iliac crest mesenchymal cells over other sources. However, further research is needed to optimize its effectiveness, considering factors such as the choice of cell source, isolation, and administration methods. Additional clinical studies are needed to evaluate its efficacy and safety in different clinical settings

Um sistema de medição de desempenho para startups incubadas

Purpose – The development of a model to measure and assess the performance of start-ups in a standard incubation process, based on 99 critical success factors identified as the most relevant for the context.Design/methodology/approach – A total of three sequential steps were developed to reach the objectives proposed (Problem overview; modeling; and usage). The model was tested on four development stage incubation processes start-ups and three maturity stage incubation processes start-ups, located at the Technological Incubator of Santa Maria (ITSM).Findings – After the modeling phase, compromising results were found for only one developed stage start-up evaluated. Meanwhile, for the maturity stage, all three evaluated start-ups are competitive. To support the strategic decision-making process, the scores obtained were stratified to diagnose which perspective may compromise the performance of each start-up.Originality/value – This research proved to be adaptable to the decision context, thus being amenable to be used in different scenarios. The model presented in this work is composed of a systematic tool suitable to support the continuous improvement and learning processes for incubated start-ups, in specific to measure and assess the performance of start-ups.Proposta – O desenvolvimento de um modelo para medir e avaliar o desempenho de startups em um processo padrão de incubação, baseado em 99 fatores críticos de sucesso identificados como os mais relevantes para o contexto.Metodologia – Um total de três etapas sequenciais foram desenvolvidas para atingir os objetivos propostos (visão geral do problema; modelagem e uso). O modelo foi testado em quatro startups em estágio de desenvolvimento e três startups em estágio de maturidade, localizados na Incubadora Tecnológica de Santa Maria (ITSM).Resultados – Após a fase de modelagem, os resultados comprometedores foram encontrados para apenas uma startup em estágio de desenvolvimento. Enquanto isso, para o estágio de maturidade, todas as três startups avaliadas são competitivas. Para apoiar o processo decisório estratégico, os escores obtidos foram estratificados para diagnosticar quais perspectivas podem comprometer o desempenho de cada startup.Originalidade – Esta pesquisa mostrou-se adaptável ao contexto de decisão, sendo, portanto, passível de ser utilizada em diferentes cenários. O modelo apresentado neste trabalho é composto por uma ferramenta sistemática adequada para apoiar a melhoria contínua e os processos de aprendizagem para startups incubadas, em específico para medir e avaliar o desempenho de startups

Genotypic characterization of toxigenic Escherichia coli isolated from pigs with postweaning diarrhea (PWD) and edema disease (ED)

El objetivo del trabajo fue caracterizar mediante PCR 47 aislamientos de Escherichia coli recuperados de 32 cerdos con diagnóstico clínico de diarrea posdestete (DPD) y de 3 cerdos con enfermedad de los edemas (ED). Sobre 44 aislamientos provenientes de cerdos con DPD, 42 (95,5 %) fueron caracterizados como E. coli enterotoxigénicos (ETEC) y 2 (4,5 %) como E. coli productores de toxina Shiga (STEC). Catorce aislamientos de ETEC (33,3 %) fueron positivos para los genes estI/estII/fedA. El genotipo más complejo fue eltA/estII/east1/faeG/aidA. Los aislamientos provenientes de cerdos con ED se clasificaron como STEC porcinos y fueron portadores de stx2e aidA. Once aislamientos (25 %) fueron portadores del gen que codifica la expresión de la adhesina AIDA-I. Sin embargo, en ningún aislamiento se detectaron los genes que codifican la expresión de las adhesinas F5, F6, F41, de intimina y de PAA. La prevención de la DPD y de la ED podría realizarse mediante el desarrollo de vacunas que generen anticuerpos contra las adhesinas de las cepas de E. coli prevalentes en Argentina.The purpose of this work was to characterize 47 Escherichia coli strains isolated from 32 pigs diagnosed with postweaning diarrhea and tree pigs with edema disease by PCR. Forty two (95.5 %) of the strains isolated from diarrheic pigs were characterized as enterotoxigenic E. coli (ETEC) and 2 (4.5 %) as Shiga toxin-producing E. coli (STEC). Fourteen (33.3 %) ETEC strains were positive for est/estII/fedA genes. The most complex genotype was eltA/estI/faeG/aidA. Strains isolated from pigs with ED were classified as porcine STEC and were stx2e/aidA carriers. Eleven (25 %) strains carried the gene encoding adhesin protein AIDA-I. However, genes coding for F5, F6, F41, intimin and Paa were not detected. The development of vaccines generating antibodies against prevalent E. coli adhesins in Argentina could be useful for the prevention of PWD and ED.Fil: Moredo, Fabiana Alicia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Cappuccio, Javier Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Insarralde, Lucas. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Perfumo, Carlos Juan. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Quiroga, María A.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Leotta, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentin

El enfoque intercultural en actividades de lectura presentes en libros de texto editados para la enseñanza del inglés a adolescentes (nivel a1)

El presente trabajo describe un proyecto de investigación, llevado a cabo en el Instituto de Formación Docente Continua de la ciudad de San Luis, que tuvo como objetivo analizar y describir en relación al enfoque intercultural las actividades de lectura en manuales para la enseñanza de la lengua-cultura extranjera inglés de nivel A1 disponibles en Argentina. Se desarrolló una investigación exploratoria-descriptiva que respondió a una lógica de investigación cualitativa. Debido a la saturación de datos, se incluyeron en la muestra compuesta por siete manuales las secuencias de lectura correspondientes a las primeras tres unidades de cada manual. Los resultados sugieren que la inclusión del enfoque intercultural tiene un rol superficial, secundario y no transversal a los contenidos, y que lengua y cultura se presentan como aspectos disociados. Las actividades que promueven la competencia comunicativa intercultural son de personalización y de búsqueda de información ampliatoria, y tienden a ser unidireccionales sin promover la reflexión intercultural

Philornis downsi (Diptera: Muscidae), an avian nest parasite invasive to the Galápagos Islands, in Mainland Ecuador

Philornis downsi (Dodge and Aitken) is a bird-parasitic muscid fly native to mainland South America that recently invaded the Galápagos Islands where it is parasitizing Darwin's finches and other land birds. This parasite was previously known only from Argentina, Brazil, and Trinidad and Tobago. The first report of P. downsi from mainland Ecuador is provided, supporting the hypothesis that the invasion route of P. downsi from its native range to the Galápagos Islands includes mainland Ecuador. Four different morphologies of pupal exuviae, which belong to different fly species, were uncovered. Dipteran puparia were found in 20% of naturally occurring nests collected in 2013 and in 27% of nests in 2014 at the two sites in western Ecuador. P. downsi accounted for 74% and 40% of the puparia in 2013 and 2014, respectively. Two new bird host species for the genus Philornis were recorded: the streak-headed woodcreeper (Lepidocolaptes souleyetii) and the fasciated wren (Campylorhynchus fasciatus). The levels of nest parasitism found were much lower than levels reported for the Galápagos Islands. Finally, parasitism of P. downsi by a parasitoid in the genus Brachymeria (Hymenoptera, Chalcididae) is documented. Overall, 9% of fly puparia collected in 2013, and 19% of those collected in 2014, had parasitoid emergence holes.Fil: Bulgarella, Mariana. University of Minnesota; Estados UnidosFil: Quiroga, Martin Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Brito Vera, Gabriel A.. Universidad de Guayaquil; EcuadorFil: Dregni, Jonathan S.. University of Minnesota; Estados UnidosFil: Cunninghame, Francesca. Charles Darwin Foundation for the Galápagos Islands; EcuadorFil: Mosquera Muñoz, Denis A.. Universidad de Guayaquil; EcuadorFil: Monje, Lucas Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Causton, Charlotte E.. Charles Darwin Foundation for the Galápagos Islands; EcuadorFil: Heimpel, George E.. University of Minnesota; Estados Unido

Efectos de las pasturas perennes en la estructura de molisoles, de la región semiárida pampeana

El presente trabajo tiene como objetivo evaluar la condición estructural de suelos influenciados por distintas prácticas de uso. El estudio se desarrolló en la Región Semiárida Pampeana sobre Paleustoles petrocálcicos, se seleccionaron suelos de textura franca, bajo 3 usos diferentes que corresponden a ensayos de larga duración: pastura permanente de pasto llorón (LL) (60 años) el cual fue considerado como línea base o de referencia; pasturas perennes: festuca+agropiro (F+A) y agropiro+alfalfa (A+A); y por último un suelo bajo uso agrícola.Current agricultural production systems mainly use annual crops with a high proportion of oilseeds in the rotation. This implied less root contribution and lower residue biomass on the soil surface, which led to organic carbon losses and weakened soil structures. The objective of the study was to evaluate the structural condition of soils influenced by different land use. The study was carried out in the Semi-arid Pampean Region on petrocalcic Paleustolls, loamy texture soils, under 3 different uses were selected that correspond to long-term trials: permanent pastures of weeping lovegrass (LL) (60 years) which was considered as baseline or reference; perennial pastures of fescue+wheatgrass (F+A) and wheatgrass+alfalfa (A+A); and finally, a land under agricultural use. Aggregate size distribution, organic matter content, weight of a thousand aggregates, aggregate porosity, structural stability, and erodible fraction were determined. These structural quality indicators showed that both perennial pastures (F+A and A+A) were closer to LL values than to agriculture. Therefore, including perennial pastures in the rotation (both grasses and legumes), contributed significantly to the structural properties determining soil quality, improving its physical and biological fertility.EEA AnguilFil: Fernandez, Romina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina. Universidad Nacional de La Pampa. Facultad de Agronomía; ArgentinaFil: Lucas, Milagros María Rosa. Universidad Nacional de La Pampa. Facultad de Agronomía; ArgentinaFil: Painemilla, Lorena de los Angeles. Universidad Nacional de La Pampa. Facultad de Agronomía; ArgentinaFil: Quiroga, Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina. Universidad Nacional de La Pampa. Facultad de Agronomía; Argentin

Genotypic characterization of toxigenic Escherichia coli isolated from pigs with postweaning diarrhea (PWD) and edema disease (ED)

El objetivo del trabajo fue caracterizar mediante PCR 47 aislamientos de Escherichia coli recuperados de 32 cerdos con diagnóstico clínico de diarrea posdestete (DPD) y de 3 cerdos con enfermedad de los edemas (ED). Sobre 44 aislamientos provenientes de cerdos con DPD, 42 (95,5 %) fueron caracterizados como E. coli enterotoxigénicos (ETEC) y 2 (4,5 %) como E. coli productores de toxina Shiga (STEC). Catorce aislamientos de ETEC (33,3 %) fueron positivos para los genes estI/estII/fedA. El genotipo más complejo fue eltA/estII/east1/faeG/aidA. Los aislamientos provenientes de cerdos con ED se clasificaron como STEC porcinos y fueron portadores de stx2e aidA. Once aislamientos (25 %) fueron portadores del gen que codifica la expresión de la adhesina AIDA-I. Sin embargo, en ningún aislamiento se detectaron los genes que codifican la expresión de las adhesinas F5, F6, F41, de intimina y de PAA. La prevención de la DPD y de la ED podría realizarse mediante el desarrollo de vacunas que generen anticuerpos contra las adhesinas de las cepas de E. coli prevalentes en Argentina.The purpose of this work was to characterize 47 Escherichia coli strains isolated from 32 pigs diagnosed with postweaning diarrhea and tree pigs with edema disease by PCR. Forty two (95.5 %) of the strains isolated from diarrheic pigs were characterized as enterotoxigenic E. coli (ETEC) and 2 (4.5 %) as Shiga toxin-producing E. coli (STEC). Fourteen (33.3 %) ETEC strains were positive for est/estII/fedA genes. The most complex genotype was eltA/estI/faeG/aidA. Strains isolated from pigs with ED were classified as porcine STEC and were stx2e/aidA carriers. Eleven (25 %) strains carried the gene encoding adhesin protein AIDA-I. However, genes coding for F5, F6, F41, intimin and Paa were not detected. The development of vaccines generating antibodies against prevalent E. coli adhesins in Argentina could be useful for the prevention of PWD and ED.Facultad de Ciencias VeterinariasInstituto de Genética Veterinari

Preliminary approach to the study of secondary lithic deposits (Río chico- Río avilés interbasin area, Tierra del fuego, Argentina)

En esta contribución se presentan los primeros resultados de un muestreo dirigido que permitió caracterizar cuantitativa y cualitativamente las potenciales fuentes secundarias de materias primas líticas utilizadas para la manufactura de artefactos en el sitio arqueológico Herradura 1. Este sitio se localiza en el noreste de Tierra del Fuego y fue ocupado por grupos cazadores-recolectores durante el Holoceno tardío. Para describir y comparar las fuentes secundarias se seleccionaron unidades de análisis donde las materias primas -que consisten en clastos sueltos- estuvieran dispuestas sobre la superficie. Los clastos fueron caracterizados de acuerdo con su génesis, tamaño de grano, homogeneidad del tamaño de grano, presencia de rasgos planares y número de fracturas. Los resultados obtenidos permiten afirmar que existen numerosas fuentes secundarias inmediatamente disponibles que contienen rocas de calidad irregular. En contraste, el análisis de los artefactos líticos recuperados en Herradura 1 indica una selección de materias primas de buena calidad para la talla, ya que se utilizaron rocas de grano fino, con tamaños de grano homogéneos y un bajo porcentaje de fracturas y rasgos planares.In this contribution the first results of a nonrandom sampling methodology applied on secondary lithic deposits are shown. Lithic sources are described quantitatively and qualitatively so as to know which raw materials were available for manufacturing stone artifacts in the archaeological site Herradura 1. This site is located in the northeastern of Tierra del Fuego and it was occupied by hunter-gatherers during the Late Holocene. In order to characterize and compare lithic secondary sources, analytical units where raw materials are present over the surface were selected. Several clasts attributes were analyzed, including geological origin, grain size, grain size homogeneity, presence of planar features and number of fractures. The results obtained confirm that although there are numerous secondary deposits immediately accessible, the knapping quality of available rocks is heterogeneous and the presence of unfavorable traits for knapping is frequently registered. In contrast, analysis of lithic artifacts recovered in H1 indicates a selection of raw materials of good quality: fine-grained rocks, with homogeneous size grain and a low percentage of fractures and planar features.Fil: Turnes, Lucas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; ArgentinaFil: Quiroga, Diego Rubén Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; ArgentinaFil: Santiago, Fernando Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; ArgentinaFil: Negre Pérez, Joan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; ArgentinaFil: Gonzalez Guillot, Mauricio Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentin

Outbreak of swine influenza in Argentina reveals a non-contemporary human H3N2 virus highly transmissible among pigs

Sporadic outbreaks of human H3N2 influenza A virus (IAV) infections in swine populations have been reported in Asia, Europe and North America since 1970. In South America, serological surveys in pigs indicate that IAVs of the H3 and H1 subtypes are currently in circulation; however, neither virus isolation nor characterization has been reported. In November 2008, an outbreak of respiratory disease in pigs consistent with swine influenza virus (SIV) infection was detected in Argentina. The current study describes the clinical epidemiology, pathology, and molecular and biological characteristics of the virus. Phylogenetic analysis revealed that the virus isolate shared nucleotide identities of 96-98% with H3N2 IAVs that circulated in humans from 2000 to 2003. Antigenically, sera from experimentally inoculated animals cross-reacted mainly with noncontemporary human-origin H3N2 influenza viruses. In an experimental infection in a commercial swine breed, the virus was of low virulence but was transmitted efficiently to contact pigs and caused severe disease when an infected animal acquired a secondary bacterial infection. This is the first report of a wholly human H3N2 IAV associated with clinical disease in pigs in South America. These studies highlight the importance of two-way transmission of IAVs and SIVs between pigs and humans, and call for enhanced influenza surveillance in the pig population worldwide.Facultad de Ciencias Veterinaria