Hyalinizing clear cell carcinoma of salivary gland: a clinicopathological study of 8 cases

Background and purpose: Hyalinizing clear cell carcinoma (HCCC) of salivary gland is a rare tumor with low-grade malignancy, which usually occurs in minor salivary glands. Due to its rarity, the tumor type is not well known to pathologists and misdiagnosis frequently occurred. The aim of this study was to investigate the histologic, immunophenotypic and molecular features of HCCC. Methods: Eight cases of salivary gland HCCC diagnosed at the Department of Pathology, Fudan University Shanghai Cancer Center from January 2015 to October 2019 were collected. Clinicopathologic characteristics were analyzed, and fluorescence in situ hybridization (FISH) assay was used to detect EWSR1 translocation in tumor tissue. Diagnostic criteria and differential diagnosis were summarized, with a review of the literature. Results: All patients were female, aged 26 to 68 years, and the tumor locations included the oral cavity, nasopharynx, parotid gland and other sites of head and neck. Microscopically, the tumors consisted mainly of clear cells and eosinophilic cells with variable proportions, arranged in nests, sheets and trabeculae. In 1 case, the tumor was composed almost entirely of eosinophilic cells; in 6 cases, obvious nuclear pseudo-inclusion bodies were seen; in 1 case, neoplastic cells were connected to the surface squamous epithelium; in 1 case, the glandular structure was seen in some areas of the tumor; in 3 cases, a sheath of lymphocyte was formed around the tumor nests; in 7 cases, there was a sclerosing or hyalinizing stromal reaction, showing desmoplastic changes; and 1 case featured stoma with myxoid changes. There was no definite keratinization or necrosis in all tumors, and mitotic figures were generally rare. Immunohistochemical staining showed that tumor cells of 8 cases were diffusely positive for AE1/AE3 protein, 7 cases expressed P63, and 7 cases focally expressed low molecular weight keratin CK7 or CAM5.2, however, S-100, calponin, PAX-8 and CD10 were all negative. All tumors showed low proliferative activity, with the Ki-67 proliferation index less than 5%. FISH test revealed EWSR1 translocation in all 8 cases. All patients demonstrated no recurrence or metastasis during the follow up ranging 4-50 months. Conclusions: HCCC of salivary gland is a low-grade malignancy occurring mainly in minor salivary glands, with characteristic histological and molecular features. This cancer type needs to be distinguished with many morphologic mimics of salivary gland. Most cases with salivary HCCC feature a good prognosis

Profilin 1 is essential for retention and metabolism of mouse hematopoietic stem cells in bone marrow

How stem cells interact with the microenvironment to regulate their cell fates and metabolism is largely unknown. Here we demonstrated that the deletion of the cytoskeleton-modulating protein profilin 1 (pfn1) in hematopoietic stem cell (HSCs) led to bone marrow failure, loss of quiescence, and mobilization and apoptosis of HSCs in vivo. A switch from glycolysis to mitochondrial respiration with increased reactive oxygen species (ROS) level was also observed in HSCs on pfn1 deletion. Importantly, treatment of pfn1-deficient mice with the antioxidant N-acetyl-l-cysteine reversed the ROS level and loss of quiescence of HSCs, suggesting that the metabolism is mechanistically linked to the cell cycle quiescence of stem cells. The actin-binding and proline-binding activities of pfn1 are required for its function in HSCs. Our study provided evidence that pfn1 at least partially acts through the axis of pfn1/Gα13/EGR1 to regulate stem cell retention and metabolism in the bone marrow

Components of the Hematopoietic Compartments in Tumor Stroma and Tumor-Bearing Mice

Solid tumors are composed of cancerous cells and non-cancerous stroma. A better understanding of the tumor stroma could lead to new therapeutic applications. However, the exact compositions and functions of the tumor stroma are still largely unknown. Here, using a Lewis lung carcinoma implantation mouse model, we examined the hematopoietic compartments in tumor stroma and tumor-bearing mice. Different lineages of differentiated hematopoietic cells existed in tumor stroma with the percentage of myeloid cells increasing and the percentage of lymphoid and erythroid cells decreasing over time. Using bone marrow reconstitution analysis, we showed that the tumor stroma also contained functional hematopoietic stem cells. All hematopoietic cells in the tumor stroma originated from bone marrow. In the bone marrow and peripheral blood of tumor-bearing mice, myeloid populations increased and lymphoid and erythroid populations decreased and numbers of hematopoietic stem cells markedly increased with time. To investigate the function of hematopoietic cells in tumor stroma, we co-implanted various types of hematopoietic cells with cancer cells. We found that total hematopoietic cells in the tumor stroma promoted tumor development. Furthermore, the growth of the primary implanted Lewis lung carcinomas and their metastasis were significantly decreased in mice reconstituted with IGF type I receptor-deficient hematopoietic stem cells, indicating that IGF signaling in the hematopoietic tumor stroma supports tumor outgrowth. These results reveal that hematopoietic cells in the tumor stroma regulate tumor development and that tumor progression significantly alters the host hematopoietic compartment

Evaluating the sustainable cultivation of 'Fuji' apples: suitable crop load and the impact of chemical thinning agents on fruit quality and transcription

The optimal load capacity provides sustainable production and high quality in 'Fuji' apple production. Determining optimal crop loads with simple and affordable thinning methods is critical for apple. We set different load capacities on different apple tree rootstocks and evaluated their effects on tree development, fruit yield and quality. The optimal load capacity for vigorous stock, dwarfing rootstock, and dwarfing interstock were 240, 90, and 100 for apples above 80 mm in diameter, respectively. To explore the optimal strategies of diverse fruit thinning agents (carbaryl, 6-BA, NAA, and Metamitron) used in the 'Fuji' apple tree, we assess from thinning agents' types, varying spraying concentrations, and application time in the most widely used dwarfing interstock. The results showed that the best use of fruit thinning agents is 2,000 mg/L carbaryl at 10 d after flowering could significantly reduce fruit set rate and improve the fruit quality in the dwarfing interstock. During the research, only the fruit thinner NAA treatment suppresses fruit development. Thus, we performed the transcriptome analysis on the NAA-treated and control fruits at 60 (FS1), 90 (FS2), 120 (FS3), and 150 (FS4) days after flowering stages to investigate the potential transcriptional regulations of NAA on fruit development and ripening. Transcriptome results showed that genes related to fruit expansion (expansin A15, expansin B3), phytohormone-related genes (HVA22C, PRE1, AHP1, etc.), fruit coloring-related genes (PAL), and many ripening-related transcription factors (EIN3, ERF, ARF, etc.) might be regulated by exogenous NAA. Our results provide an important reference for the sustainable production and optimal use of fruit thinning agents

New Strategy for the Design of Anti-Corrosion Coatings in Bipolar Plates Based on Hybrid Organic–Inorganic Layers

As a star material in conducting polymers, a polypyrrole coating was assembled onto the surface of 316 stainless steel by an electrochemical method. In the next step, the composite layer consisting of carbon nitride nanosheets (CNNS) and polymethyl methacrylate (PMMA) was sprayed. The corrosion manner of composite coatings in a simulated proton-exchange membrane fuel cell (PEMFC) environment was evaluated. The results show that the final coating generated at a voltage of 1.0 has demonstrated the optimized corrosion resistance. The polypyrrole layer improves the corrosion resistance of the stainless steel substrate, and the CNNS/PMMA coating further strengthens the physical barrier effect of the coating in corrosive solutions

Extension of Novel Lanthanide Luminescent Mesoporous Nanostructures to Detect Fluoride

A novel polydentate type ligand derived from <i>N</i>²,<i>N</i>⁶-bis­(4,4-diethoxy-9-oxo-3-oxa-8,10-diaza-4-siladodecan-12-yl)­pyridine-2,6-dicarboxamide (<b>L</b>) has been designed, and it played essential roles in the assembly of new organic–inorganic functional materials. First, its multiple amide groups would coordinate to lanthanide ions firmly and transfer the absorbed energy to both Eu­(III) and Tb­(III) simultaneously. Second, the hydrogen-bond donor units showed strong affinity to guest anion (F^–). Third, the two silylated arms could induce the formation of sol–gel derived siloxane hybrid materials. Following this idea, two lanthanide luminescent amorphous particles (<b>ASNs-Eu</b> and <b>ASNs-Tb</b>) have been prepared for the recognition of fluoride ions. Further modification of the synthesis method and transformation to mesoporous network (<b>MSNs-Eu</b> and <b>MSNs-Tb</b>) led to much enhanced thermostabilities, larger specific surface area (from 78.5 to 515 m² g^–1 for Eu­(III); 89.6 to 487 m² g^–1 for Tb­(III)), and lower detection limits (2.5 × 10^–8 M for <b>MSNs-Eu</b> and 3.4 × 10^–8 M for <b>MSNs-Tb</b>) for the fluoride ion

Lanthanide Molecular Species Generated Fe3O4@SiO2-TbDPA Nanosphere for the Efficient Determination of Nitrite

The presence of nitrite (NO2&minus;) in water and food leads to serious problems in public health and the environment. Therefore, it is important to develop a rapid and efficient method for the selective detection of NO2&minus;. In this work, the synthesis and characterization of magnetic Fe3O4@SiO2-TbDPA nanoprobe have been carried out. The Fe3O4@SiO2-TbDPA aqueous solution exhibits a strong green emission. Due to the addition of various concentrations of NO2&minus; (0&ndash;100 &mu;M), the fluorescence intensity has been suppressed. The nanoprobe Fe3O4@SiO2-TbDPA exhibits excellent selectivity and sensitivity toward NO2&minus; ions. Excellent linearity is obtained in the range of 5&ndash;80 &mu;M with a detection limit of 1.03 &mu;M. Furthermore, the presence of magnetic Fe3O4 nanoparticles in Fe3O4@SiO2-TbDPA nanospheres will also facilitate the effective separation of Fe3O4@SiO2-TbDPA from the aqueous solution. Our proposed strategy is expected to fabricate an organic-inorganic hybrid magnetic nanomaterial and can be used as an efficient sensor. It has been shown that this new strategy has numerous advantages, such as high stability, selectivity, and simplicity of operation. It demonstrates great potential for simple and convenient NO2&minus; detection. It may expand to a variety of ranges in environmental monitoring and biomedical fields

Aggregation Induced Emission Mediated Controlled Release by Using a Built-In Functionalized Nanocluster with Theranostic Features

We report biological evaluation of a novel nanoparticle delivery system based on 1,1,2-triphenyl-2-(<i>p-</i>hydroxyphenyl)-ethene (TPE-OH, compound <b>1</b>), which has tunable aggregation-induced emission (AIE) characteristics. Compound <b>1</b> exhibited no emission in DMSO. In aqueous media, compound <b>1</b> aggregated, and luminescence was observed. The novel membrane–cytoplasm–nucleus sequential delivery strategy could induce apoptosis in four different kinds of cancer cells (including three adherent cell lines and one suspension cell line). The nanoparticles remained in the cytoplasm with intense blue emissions, whereas doxorubicin was observed in the nucleus with striking red luminescence. The nanoassembly was internalized in cells through an energy-dependent process. Three sorts of chemical inhibitors were used to clarify the endocytosis mechanism based on the AIE type prodrug. Furthermore, we have developed the first AIE theranostic system where drug targeting and release have been applied in an animal model

Refining Urban Built-Up Area via Multi-Source Data Fusion for the Analysis of Dongting Lake Eco-Economic Zone Spatiotemporal Expansion

Rapid urbanization has given rise to serious urban problems. It is crucial to understand the urbanization process to accurately and quickly identify boundary changes in urban built-up areas and implement planning schemes and adjustments in scientific and effective ways. This study proposes a new method to automate and refine the extraction of urban built-up areas by using Landsat and nighttime light (NTL) imagery. The urban agglomeration of Dongting Lake Ecological Economic Zone (UADLEEZ) Landsat data are mapped to NTL data using resampling, superpixel segmentation, and assigning the blank part with the Euclidean distance method. We then compared our findings with those produced via traditional threshold extraction methods. In total, 33 built-up areas of UADLEEZ boundary maps were produced between 1992 and 2018. Thus, we reached the following conclusions: (1) the urban built-up areas obtained via our proposed method are finer than those obtained via other threshold extraction methods; (2) we applied the extraction method to UADLEEZ, and analyzed the expansion of the urban agglomeration based on expansion scale, gravity center offset, and landscape pattern index, the analysis of expansion process is consistent with the actual situation; (3) the proposed method can be used to draw long-term dynamic maps of urban extents in units of years, and the results can be used to update the existing products. This study can serve as a reference for future urban planning, and provide both adjustment programs for relevant departments, and an objective basis for governmental decision-making