Exploring the visualisation of the cervicothoracic junction in lateral spine radiography using high dynamic range techniques

The C7/T1 junction is an important landmark for spinal injuries. It is traditionally difficult to visualise in a lateral X-ray image due to the rapid change in the bodys anatomy at the level of the junction, where the shoulders cause a large increase in attenuation. To explore methods of enhancing the appearance of this important area, lateral radiographs of a shoulder girdle phantom were subjected to high dynamic range (HDR) processing and tone mapping. A shoulder girdle phantom was constructed using Perspex, shoulder girdle and vertebral bones and water to reproduce the attenuation caused by soft tissue. The design allowed for the removal of the shoulder girdle in order for the cervical vertebrae to be imaged separately. HDR was explored for single and dual-energy X-ray images of the phantom. In the case of single-image HDR, the HDR image of the phantom without water was constructed by combining images created with varying contrast windows throughout the contrast range of an X-ray image. It was found that an overlap of larger contrast windows with a lower number of images performed better than smaller contrast windows and more images when creating an HDR to be tone mapped. Poor results on the phantom without water precluded further testing of single-image HDR on images of the phantom with water, which would have higher attenuation. Dual energy HDR image construction explored images of the phantom both with and without water. A set of images acquired at lower attenuation (phantom without water) was used to evaluate the performance of the various tone mapping algorithms. The tone mapping was then performed on the phantom images containing water. These results showed how each tone mapping algorithm differs and the effects of global vs. local processing. The results revealed that the built-in MatLab algorithm, based on an improved Ward histogram adjustment approach, produces the most desirable result. None of the HDR tone mapped images produced were diagnostically useful. Signal to noise ratio (SNR) analysis was performed on the cervical region of the HDR tone mapped image. It used the scan of the phantom without the shoulder girdle obstruction (imaged under the same conditions) as a reference image. The SNR results quantitatively show that the selection of exposure values affects the visualisation of the tone mapped image. The highest SNR was produced for the 100 - 120 kV dual energy X-ray image pair. The study was limited by the range of HDR image construction techniques employed and the tone mapping algorithms explored. Future studies could explore other HDR image construction techniques and the combination of global and local tone mapping algorithms. Furthermore, the phantom can be replaced by a cadaver for algorithm testing under more realistic conditions

A Case Study: Law and Emotions Within the Kingdom of the Netherlands

Whether you are a Christian or not, you cannot deny the truth of the proverb “[a] brother offended is more unyielding than a strong city, and quarrelling is like the bars of a castle,”1 especially when you study the constitutional relationship between the Netherlands and its former colonies Aruba, Curacao, and St. Maarten. The Netherlands, Aruba, Curacao and St. Maarten are four countries that together constitute the Kingdom of the Netherlands.2 These countries feel so wronged by one another that emotions often take over. In July 2014, for instance, the Prime Minister of Aruba desperately went on a hunger strike because he felt that the autonomy of Aruba had been illegally infringed upon as the Kingdom Government ordered the Governor of Aruba not to sign the country’s budget. The reasoning behind this order was in response to an opinion of the Kingdom Government that the debt had grown explosively and that this budget aggravated the problem. Subsequently, the Prime Minister of Aruba believed that the dispute settlement procedure between the Kingdom, ‘central’ (predominantly Dutch) government, and ‘local’ government was useless.3 He felt that the Dutch government would be overrepresented in this procedure, and he was afraid that the Dutch government would maintain its stance.4 The Dutch government urged for reasonableness.

A Case Study: Law and Emotions Within the Kingdom of the Netherlands

Whether you are a Christian or not, you cannot deny the truth of the proverb “[a] brother offended is more unyielding than a strong city, and quarrelling is like the bars of a castle,”1 especially when you study the constitutional relationship between the Netherlands and its former colonies Aruba, Curacao, and St. Maarten. The Netherlands, Aruba, Curacao and St. Maarten are four countries that together constitute the Kingdom of the Netherlands.2 These countries feel so wronged by one another that emotions often take over. In July 2014, for instance, the Prime Minister of Aruba desperately went on a hunger strike because he felt that the autonomy of Aruba had been illegally infringed upon as the Kingdom Government ordered the Governor of Aruba not to sign the country’s budget. The reasoning behind this order was in response to an opinion of the Kingdom Government that the debt had grown explosively and that this budget aggravated the problem. Subsequently, the Prime Minister of Aruba believed that the dispute settlement procedure between the Kingdom, ‘central’ (predominantly Dutch) government, and ‘local’ government was useless.3 He felt that the Dutch government would be overrepresented in this procedure, and he was afraid that the Dutch government would maintain its stance.4 The Dutch government urged for reasonableness.

Using legacy data to reconstruct the past? Rescue, rigour and reuse in peatland geochronology

There is a growing interest in the rescue and reuse of data from past studies (so-called legacy data). Data loss is alarming, especially where natural archives are under threat, such as peat deposits. Here we develop a workflow for reuse of legacy radiocarbon dates in peatland studies, including a rigorous quality assessment that can be tailored to specific research questions and study regions. A penalty is assigned to each date based on criteria that consider taphonomic quality (i.e., sample provenance) and dating quality (i.e., sample material and method used). The weights of quality criteria may be adjusted based on the research focus, and resulting confidence levels may be used in further analyses to ensure robustness of conclusions. We apply the proposed approach to a case study of a (former) peat landscape in the Netherlands, aiming to reconstruct the timing of peat initiation spatially. Our search yielded 313 radiocarbon dates from the 1950s to 2019. Based on the quality assessment, the dates—of highly diverse quality—were assigned to four confidence levels. Results indicate that peat initiation for the study area first peaked in the Late Glacial (~14,000 cal years BP), dropped during the Boreal (~9,500 cal years BP) and showed a second peak in the Subboreal (~4,500 cal years BP). We tentatively conclude that the earliest peak was mostly driven by climate (Bølling–Allerød interstadial), whereas the second was probably the result of Holocene sea level rise and related groundwater level rise in combination with climatic conditions (hypsithermal). Our study highlights the potential of legacy data for palaeogeographic reconstructions, as it is cost-efficient and provides access to information no longer available in the field. However, data retrieval may be challenging, and reuse of data requires that basic information on location, elevation, stratigraphy, sample and laboratory analysis are documented irrespective of the original research aims

Predictors of functional dependency in Parkinson’s disease

Financial disclosures/conflicts of interest: Dr Macleod was funded by a Clinical Academic Fellowship from the Chief Scientist Office of the Scottish Government and received grant funding from Parkinson’s UK, the Wellcome Trust, University of Aberdeen, and NHS Grampian endowments relating to this research. Dr Counsell received grant funding from Parkinson’s UK, National Institute for Health Research, the Scottish Chief Scientist Office, the BMA Doris Hillier award, RS Macdonald Trust, the BUPA Foundation, NHS Grampian endowments and SPRING relating to this research. We declare we have no conflicts of interest. Financial support: This study was funded by Parkinson’s UK, the Scottish Chief Scientist Office, NHS Grampian endowments, the BMA Doris Hillier award, RS Macdonald Trust, the BUPA Foundation, and SPRING.  Peer reviewedPublisher PD

Nitric oxide is involved in acetylcholinesterase inhibitor-induced myopathy in rats

ABSTRACT Excess activation of muscle nicotinic acetylcholine receptors due to genetic mutations, as seen in slow channel congenital myasthenic syndrome, or acetylcholinesterase (AChE) inhibition results in muscle cell degeneration. Our recent work showed that nitric oxide synthase (NOS) inhibitors prevent nicotine-induced muscle cell death in culture. In the present study, we examined the effects of NOS inhibition on nicotinic receptor-mediated myopathy in vivo. Rats injected with the AChE inhibitor paraoxon demonstrate a 90-fold increase in the number of dying muscle cells compared with control as evidenced histologically by centralized nuclei and the presence of degenerating profiles. Coadministration of the nonspecific NOS inhibitor nitro-L-arginine methyl ester or the neuronal NOS-specific inhibitor 7-nitroindazole dramatically reduced the presence of such degenerating profiles to ϳ20% of that seen with paraoxon alone. These results show that inhibition of NOS, as well as neuronal NOS, significantly reduces AChE inhibitor-induced muscle cell degeneration, suggesting that increased nitric oxide production mediates such myopathy

Cortical nNOS neurons co-express the NK1 receptor and are depolarized by Substance P in multiple mammalian species

We have previously demonstrated that Type I neuronal nitric oxide synthase (nNOS)-expressing neurons are sleep-active in the cortex of mice, rats, and hamsters. These neurons are known to be GABAergic, to express Neuropeptide Y (NPY) and, in rats, to co-express the Substance P (SP) receptor NK1, suggesting a possible role for SP in sleep/wake regulation. To evaluate the degree of co-expression of nNOS and NK1 in the cortex among mammals, we used double immunofluorescence for nNOS and NK1 and determined the anatomical distribution in mouse, rat, and squirrel monkey cortex. Type I nNOS neurons co-expressed NK1 in all three species although the anatomical distribution within the cortex was species-specific. We then performed in vitro patch clamp recordings in cortical neurons in mouse and rat slices using the SP conjugate tetramethylrhodamine-SP (TMR-SP) to identify NK1-expressing cells and evaluated the effects of SP on these neurons. Bath application of SP (0.03–1 μM) resulted in a sustained increase in firing rate of these neurons; depolarization persisted in the presence of tetrodotoxin. These results suggest a conserved role for SP in the regulation of cortical sleep-active neurons in mammals

Anthropogenic drivers for exceptionally large meander formation during the Late Holocene

Large-amplitude meanders may form in low-energy rivers despite generally limited mobility in theses systems. Exceptionally large meanders which even extend beyond the valley sides have developed in the Overijsselse Vecht river (the Netherlands) between ca. 1400 CE (Common Era) and the early 1900s, when channelization occurred. Previous studies have attributed the enhanced lateral dynamics of this river to changes in river regime due to increased discharges, reflecting climate and/or land-use alterations in the catchment. This paper focuses on local aspects that may explain why exceptionally large meanders developed at specific sites. Through an integrated analysis based on archaeological, historical, and geomorphological data along with optically stimulated luminescence dating, we investigated the relative impact of three direct and indirect anthropogenic causes for the local morphological change and enhanced lateral migration rates: (1) lack of strategies to manage fluvial erosion; (2) a strong increase in the number of farmsteads and related intensified local land use from the High Middle Ages onwards; and (3) (human-induced) drift-sand activity directly adjacent to the river bends, causing a change in bank stability. Combined, these factors led locally to meander amplitudes well beyond the valley sides. Lessons learned at this site are relevant for management and restoration of meandering rivers in similar settings elsewhere, particularly in meeting the need to estimate spatial demands of (restored) low-energy fluvial systems and manage bank erosion.</p