2,999 research outputs found
One-dimensional Topological Edge States of Bismuth Bilayers
The hallmark of a time-reversal symmetry protected topologically insulating
state of matter in two-dimensions (2D) is the existence of chiral edge modes
propagating along the perimeter of the system. To date, evidence for such
electronic modes has come from experiments on semiconducting heterostructures
in the topological phase which showed approximately quantized values of the
overall conductance as well as edge-dominated current flow. However, there have
not been any spectroscopic measurements to demonstrate the one-dimensional (1D)
nature of the edge modes. Among the first systems predicted to be a 2D
topological insulator are bilayers of bismuth (Bi) and there have been recent
experimental indications of possible topological boundary states at their
edges. However, the experiments on such bilayers suffered from irregular
structure of their edges or the coupling of the edge states to substrate's bulk
states. Here we report scanning tunneling microscopy (STM) experiments which
show that a subset of the predicted Bi-bilayers' edge states are decoupled from
states of Bi substrate and provide direct spectroscopic evidence of their 1D
nature. Moreover, by visualizing the quantum interference of edge mode
quasi-particles in confined geometries, we demonstrate their remarkable
coherent propagation along the edge with scattering properties that are
consistent with strong suppression of backscattering as predicted for the
propagating topological edge states.Comment: 15 pages, 5 figures, and supplementary materia
Experimental Measurement of the Berry Curvature from Anomalous Transport
Geometrical properties of energy bands underlie fascinating phenomena in a
wide-range of systems, including solid-state materials, ultracold gases and
photonics. Most famously, local geometrical characteristics like the Berry
curvature can be related to global topological invariants such as those
classifying quantum Hall states or topological insulators. Regardless of the
band topology, however, any non-zero Berry curvature can have important
consequences, such as in the semi-classical evolution of a wave packet. Here,
we experimentally demonstrate for the first time that wave packet dynamics can
be used to directly map out the Berry curvature. To this end, we use optical
pulses in two coupled fibre loops to study the discrete time-evolution of a
wave packet in a 1D geometrical "charge" pump, where the Berry curvature leads
to an anomalous displacement of the wave packet under pumping. This is both the
first direct observation of Berry curvature effects in an optical system, and,
more generally, the proof-of-principle demonstration that semi-classical
dynamics can serve as a high-resolution tool for mapping out geometrical
properties
Circumstellar discs: What will be next?
This prospective chapter gives our view on the evolution of the study of
circumstellar discs within the next 20 years from both observational and
theoretical sides. We first present the expected improvements in our knowledge
of protoplanetary discs as for their masses, sizes, chemistry, the presence of
planets as well as the evolutionary processes shaping these discs. We then
explore the older debris disc stage and explain what will be learnt concerning
their birth, the intrinsic links between these discs and planets, the hot dust
and the gas detected around main sequence stars as well as discs around white
dwarfs.Comment: invited review; comments welcome (32 pages
Physical mapping integrated with syntenic analysis to characterize the gene space of the long arm of wheat chromosome 1A
Background: Bread wheat (Triticum aestivum L.) is one of the most important crops worldwide and its production faces pressing challenges, the solution of which demands genome information. However, the large, highly repetitive hexaploid wheat genome has been considered intractable to standard sequencing approaches. Therefore the International Wheat Genome Sequencing Consortium (IWGSC) proposes to map and sequence the genome on a chromosome-by-chromosome basis.
Methodology/Principal Findings: We have constructed a physical map of the long arm of bread wheat chromosome 1A using chromosome-specific BAC libraries by High Information Content Fingerprinting (HICF). Two alternative methods (FPC and LTC) were used to assemble the fingerprints into a high-resolution physical map of the chromosome arm. A total of 365 molecular markers were added to the map, in addition to 1122 putative unique transcripts that were identified by microarray hybridization. The final map consists of 1180 FPC based or 583 LTC based contigs. Conclusions/Significance: The physical map presented here marks an important step forward in mapping of hexaploid bread wheat. The map is orders of magnitude more detailed than previously available maps of this chromosome, and the assignment of over a thousand putative expressed gene sequences to specific map locations will greatly assist future functional studies. This map will be an essential tool for future sequencing of and positional cloning within chromosome 1A
Topological Photonics
Topology is revolutionizing photonics, bringing with it new theoretical
discoveries and a wealth of potential applications. This field was inspired by
the discovery of topological insulators, in which interfacial electrons
transport without dissipation even in the presence of impurities. Similarly,
new optical mirrors of different wave-vector space topologies have been
constructed to support new states of light propagating at their interfaces.
These novel waveguides allow light to flow around large imperfections without
back-reflection. The present review explains the underlying principles and
highlights the major findings in photonic crystals, coupled resonators,
metamaterials and quasicrystals.Comment: progress and review of an emerging field, 12 pages, 6 figures and 1
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Associations of sitting accumulation patterns with cardio-metabolic risk biomarkers in Australian adults
Backgroun
Combinatorial CRISPR-Cas9 screens for de novo mapping of genetic interactions.
We developed a systematic approach to map human genetic networks by combinatorial CRISPR-Cas9 perturbations coupled to robust analysis of growth kinetics. We targeted all pairs of 73 cancer genes with dual guide RNAs in three cell lines, comprising 141,912 tests of interaction. Numerous therapeutically relevant interactions were identified, and these patterns replicated with combinatorial drugs at 75% precision. From these results, we anticipate that cellular context will be critical to synthetic-lethal therapies
Serum microRNA array analysis identifies miR-140-3p, miR-33b-3p and miR-671-3p as potential osteoarthritis biomarkers involved in metabolic processes.
Background: MicroRNAs (miRNAs) in circulation have emerged as promising biomarkers. In this study, we aimed to identify a circulating miRNA signature for osteoarthritis (OA) patients and in combination with bioinformatics analysis to evaluate the utility of selected differentially expressed miRNAs in the serum as potential OA biomarkers. Methods: Serum samples were collected from 12 primary OA patients, and 12 healthy individuals were screened using the Agilent Human miRNA Microarray platform interrogating 2549 miRNAs. Receiver Operating Characteristic (ROC) curves were constructed to evaluate the diagnostic performance of the deregulated miRNAs. Expression levels of selected miRNAs were validated by quantitative real-time PCR (qRT-PCR) in all serum and in articular cartilage samples from OA patients (n = 12) and healthy individuals (n = 7). Bioinformatics analysis was used to investigate the involved pathways and target genes for the above miRNAs. Results: We identified 279 differentially expressed miRNAs in the serum of OA patients compared to controls. Two hundred and five miRNAs (73.5%) were upregulated and 74 (26.5%) downregulated. ROC analysis revealed that 77 miRNAs had area under the curve (AUC) > 0.8 and p < 0.05. Bioinformatics analysis in the 77 miRNAs revealed that their target genes were involved in multiple signaling pathways associated with OA, among which FoxO, mTOR, Wnt, pI3K/akt, TGF-β signaling pathways, ECM-receptor interaction, and fatty acid biosynthesis. qRT-PCR validation in seven selected out of the 77 miRNAs revealed 3 significantly downregulated miRNAs (hsa-miR-33b-3p, hsa-miR-671-3p, and hsa-miR-140-3p) in the serum of OA patients, which were in silico predicted to be enriched in pathways involved in metabolic processes. Target-gene analysis of hsa-miR-140-3p, hsa-miR-33b-3p, and hsa-miR-671-3p revealed that InsR and IGFR1 were common targets of all three miRNAs, highlighting their involvement in regulation of metabolic processes that contribute to OA pathology. Hsa-miR-140-3p and hsa-miR-671-3p expression levels were consistently downregulated in articular cartilage of OA patients compared to healthy individuals. Conclusions: A serum miRNA signature was established for the first time using high density resolution miR-arrays in OA patients. We identified a three-miRNA signature, hsa-miR-140-3p, hsa-miR-671-3p, and hsa-miR-33b-3p, in the serum of OA patients, predicted to regulate metabolic processes, which could serve as a potential biomarker for the evaluation of OA risk and progression.Peer reviewedFinal Published versio
Evidence for a role of TRIB3 in the regulation of megakaryocytopoiesis
Megakaryocytopoiesis is a complex differentiation process driven by the hormone
thrombopoietin by which haematopoietic progenitor cells give rise to
megakaryocytes, the giant bone marrow cells that in turn break down to form blood
platelets. The Tribbles Pseudokinase 3 gene (TRIB3) encodes a pleiotropic protein
increasingly implicated in the regulation of cellular differentiation programmes.
Previous studies have hinted that TRIB3 could be also involved in
megakaryocytopoiesis but its role in this process has so far not been investigated.
Using cellular model systems of haematopoietic lineage differentiation here we
demonstrate that TRIB3 is a negative modulator of megakaryocytopoiesis. We found
that in primary cultures derived from human haematopoietic progenitor cells,
thrombopoietin-induced megakaryocytic differentiation led to a time and dosedependent
decrease in TRIB3 mRNA levels. In the haematopoietic cell line UT7/mpl,
silencing of TRIB3 increased basal and thrombopoietin-stimulated megakaryocyte
antigen expression, as well as basal levels of ERK1/2 phosphorylation. In primary
haematopoietic cell cultures, silencing of TRIB3 facilitated megakaryocyte
differentiation. In contrast, over-expression of TRIB3 in these cells inhibited the
differentiation process. The in-vitro identification of TRIB3 as a negative regulator of
megakaryocytopoiesis suggests that in-vivo this gene could be important for the
regulation of platelet production
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