KUALITAS DAMAR POHON POOTI (HOPEA GREGARIA) BERDASARKAN UJI VISUAL, BILANGAN ASAM, DAN KADAR ABU: Quality of Pooti Dammar Resin (Hopea gregaria) Based on Visual Test, Acid Number, and Ash Content

Pooti (Hopea gregaria) is an endemic plant in Southeast Sulawesi that produces resin. But until now it has not been used because information about the quality of pooti resin has never been available. Therefore, this study aims to test the quality of pooti resin based on visual, acid number and ash content test. Pooti sap taken is on a tree with a diameter of 30 cm and a visual test is carried out based on SNI 2900-1-2012 and SNI 2900.2: 2013 for laboratory tests. The result is pooti resin with a clear yellowish color with a chunk size> 3x3 cm2, based on SNI 2900-1-2012 classified as Quality Class A. While the ash content value is 0.7% and the acid number is 29. This value indicates the ash content and acid number based on Laboratory tests are classified as quality class II based on SNI 2900.2: 2013.Pooti (Hopea gregaria) adalah tergolong tanaman endemik Sulawesi Tenggara yang menghasilkan damar. Tetapi sampai saat ini belum dimanfaatkan karena informasi tentang kualitasnya belum pernah ada.Oleh karena itu penelitian ini bertujuan untuk melihat kualitas damar Pooti berdasarkan uji visual , uji bilangan asam dan kadar abu. Metode penelitian yang digunakan adalah berdasarkan SNI  2900-1-2012 untuk uji visual dan SNI 2900.2:2013 untuk uji laboratorium. Hasilnya adalah damar Pooti memiliki warna bening kekuningan dengan ukuran bongkahan > 3x3 cm , berdasarkan SNI 2900-1-2012   tergolong kelas Mutu A. Sementara kadar abu nilainya 0,7% dan bilangan asam adalah 29. Nilai tersebut menunjukkan kadar abu berdasarkan uji laboratorium adalah tergolong kelas mutu II berdasarkan SNI 2900.2:201

Cycloartane-Type Triterpenes and Botanical Origin of Propolis of Stingless Indonesian Bee Tetragonula sapiens

This study clarifies the chemical constituents and botanical origin of Tetragonula sapiens Cockerell bee propolis collected from Southeast Sulawesi, Indonesia. Propolis samples and resin of Mangifera indica were extracted with 99% ethanol to obtain an ethanol extract of propolis (EEP) and an ethanol extract of M. indica resin (EEM). Column chromatography, thin-layer chromatography (TLC), and high-performance liquid chromatography (HPLC) were developed and used for the separation and isolation of compounds from the ether-soluble fraction. The structure of the compounds was determined by nuclear magnetic resonance (NMR) spectroscopic analysis, and their molecular weight analyzed by gas chromatography–mass spectrometry (GC–MS). The HPLC chromatogram of the EEP was then compared with the HPLC chromatogram of EEM to investigate the botanical origin of propolis. Five compounds were isolated from the EEP, and their structures were determined as mangiferolic acid, cycloartenol, ambonic acid, mangiferonic acid, and ambolic acid, which are cycloartane-type triterpenes. The characteristic peak of the HPLC chromatograms of EEP and EEM showed a similar pattern, which is that the main components of propolis were also found in M. indica resin. These results suggested that the propolis from Southeast Sulawesi was rich in cycloartane-type triterpenes, and the plant source of the propolis could be Mangifera indica (mango)

ANTIBACTERIAL ACTIVITY OF OIL EXTRACT OF TRIGONA PROPOLIS

Objective: This research is aimed to determine antibacterial activity of some solvents of raw propolis extracts of Trigona sp from Sulawesi, Indonesia and compare it with antibacterial activities of ethanolic and aqueous extracts.Methods: Propolis samples were extracted with water, ethanol, propylene glycol, olive oil, and Virgin Coconut Oil (VCO). An agar-well diffusion assay was used to evaluate the antimicrobial potential of propolis against Escherichia coli, Salmonella thypi, and Staphylococcus aureus.Results: The oily extract of propolis showed a potent antibacterial activity compared to the ethanol extracts against Staphylococcus aureus and Esherichia coli. Inhibition zone of Olive Oil Extracts of Propolis on S. aureus was higher (22.4 mm) than Ethanolic extracts and Water Extracts. Inhibition zone of Virgin Coconut Oil (VCO) propolis extract and Olive oil extract on E. coli were 9.5 mm and 9.3 mm, respectively. The oily extracts also showed higher action against E. coli compared with the ethanol extracts and propylene extracts.Conclusion: The propolis extracts obtained with Virgin Coconut Oil (VCO) and olive oil as solvent have higher antibacterial activity than the ethanolic extracts. So the VCO and Olive Oil can be used to extract raw propolis.Â

KAMPUNG KELOR SEBAGAI UPAYA KONSERVASI LAHAN TERDEGRADASI PERKOTAAN DAN MENINGKATKAN PENDAPATAN MASYARAKAT DI MASA NEW NORMAL DI KOTA KENDARI

Tujuan pelaksanaan pengabdian kepada masyarakat ini adalah untuk membangun kampung kelor sebagai upaya konservasi lahan terdegradasi perkotaan dan meningkatkan pendapatan masyarakat  di masa new normal di Kelurahan Petoaha Kota Kendari. Metode yang digunakan adalah penyuluhan dan bimbingan teknis meliputi perencanaan mulai dari  survei lokasi, perijinan dan wawancara, pelaksanaan meliputi penyuluhan, pembibitan dan budidaya kelor serta evaluasi untuk meminimalisir kelemahan dan hambatan selama kegiatan berlangsung. Hasil analisis menunjukkan bahwa setelah dilakukan penyuluhan dan bimbingan teknis pengetahuan dan kapasitas masyarakat mengalami peningkatan terkait manfaat kelor dan teknik budidayanya mulai dari pengumpulan benih, seleksi benih, perkecambahan benih, penanaman sampai pemeliharaan. Selain itu masyarakat memperoleh manfaat ekonomi dari program pengabdian kepada masyarakat terintegrasi KKN-Temati

KARAKTERISTIK SARANG DAN TUMBUHAN SUMBER GETAH PROPOLIS LEBAH TAK BERSENGAT (Stingless Bee) DARI BUTON UTARA

This study aims to determine the bee species, nest characteristics and identify the stingless bee propolis plant source from Kulisusu District, North Buton Regency. This research uses the purposive sampling method in searching nests, and the data is processed descriptively. Samples of stingless bees were taken from four hives from three sampling locations. The characteristics of the nest observed were the shape of the nest entrance, color and size (length and diameter), and the type of entrance opening. The stingless bees found belonged to the genus Tetragonula and consist of two species, Tetragonula sapiens, and Tetragonula fuscobalteata. The shape of the nest entrance varies (without funnel, short funnel, and cylindrical funnel) with black, brown, or blackish-brown color. The diameter of the nest entrance was between 3 to 5 cm with the type of opening (triangular, irregular, and oval). Nine plants were identified as sources of propolis in each nest, and two of them, Anacardium occidentale (cashews) and Schleichera oleosa (Kesambi), were found in all observed nests