Endothelio- and cardioprotective effects of vitamin B₆ and folic acid in modelling methionine-induced hyperhomocysteinemia

The endotelio- and cardioprotective effects of vitamin B₆ (2 mg/kg) and folic acid (0.2mg/kg) upon modeling of methionine-induced hyperhomocysteinemia via methionine intragastric administration at a dose of 3 g/kg were studie

Lipid distress-syndrome and prospects of its correction by statines

Endothelial dysfunction in peritonitis: The formed concept of lipid distress syndrome (LDS) allows us to develop a working hypothesis on the key role of endothelial dysfunction in the aggressive development of atherosclerosis. The role of vascular endothelium in atherosclerosis: The process of NO production from L-arginine through eNOS involving tetrahydrobiopterin (BH4) is discussed. With the degradation of BH4 along the free radical path, an "eNOS uncouplation" (uncoupled eNOS). The clinical role of statins: Statins manifests itself by inhibiting the enzyme 3-hydroxy- 3-methylglutaryl-coenzyme A (HMG-CoA reductase). Many large, randomized clinical trials have shown that lipid-lowering strategies that include statins have an anti-atherogenic potentia

Endothelium and cardioprotective effects of hmg-co-a-reductase in combination with l-arginine In endothelial dysfunction modeling

Using the combined application of L-arginine with HMG-Co-A reductase inhibitors simvastatin, atorvastatin, rosuvastatin and nanoparticulated rosuvastatin on the background of modeling of sepsis-induced disease through the introduction of strain 603 Staphylococcus aureus shows endotelio- and cardioprotective effect

Large enhancement of deuteron polarization with frequency modulated microwaves

We report a large enhancement of 1.7 in deuteron polarization up to values of 0.6 due to frequency modulation of the polarizing microwaves in a two liters polarized target using the method of dynamic nuclear polarization. This target was used during a deep inelastic polarized muon-deuteron scattering experiment at CERN. Measurements of the electron paramagnetic resonance absorption spectra show that frequency modulation gives rise to additional microwave absorption in the spectral wings. Although these results are not understood theoretically, they may provide a useful testing ground for the deeper understanding of dynamic nuclear polarization.Comment: 10 pages, including the figures coming in uuencoded compressed tar files in poltar.uu, which also brings cernart.sty and crna12.sty files neede

Lipid distress-syndrome and prospects of its correction by statines

Endothelial dysfunction in peritonitis: The formed concept of lipid distress syndrome (LDS) allows us to develop a working hypothesis on the key role of endothelial dysfunction in the aggressive development of atherosclerosis. The role of vascular endothelium in atherosclerosis: The process of NO production from L-arginine through eNOS involving tetrahydrobiopterin (BH4) is discussed. With the degradation of BH4 along the free radical path, an "eNOS uncouplation" (uncoupled eNOS). The clinical role of statins: Statins manifests itself by inhibiting the enzyme 3-hydroxy- 3-methylglutaryl-coenzyme A (HMG-CoA reductase). Many large, randomized clinical trials have shown that lipid-lowering strategies that include statins have an anti-atherogenic potentia

Endothelio- and cardioprotective effects of vitamin B₆ and folic acid in modelling methionine-induced hyperhomocysteinemia

The endotelio- and cardioprotective effects of vitamin B₆ (2 mg/kg) and folic acid (0.2mg/kg) upon modeling of methionine-induced hyperhomocysteinemia via methionine intragastric administration at a dose of 3 g/kg were studie

Endothelium and cardioprotective effects of hmg-co-a-reductase in combination with l-arginine In endothelial dysfunction modeling

Using the combined application of L-arginine with HMG-Co-A reductase inhibitors simvastatin, atorvastatin, rosuvastatin and nanoparticulated rosuvastatin on the background of modeling of sepsis-induced disease through the introduction of strain 603 Staphylococcus aureus shows endotelio- and cardioprotective effect

GENE POLYMORPHISM OF ENDOTHELIAL NITRIC OXIDE SYNTHASE IN PATIENTS WITH STABLE ANGINA PECTORIS AND ITS SIGNIFICANCE IN THE MANIFESTATION OF GENOPROTECTIVE PROPERTIES OF MELDONIUM

Introduction: Personalized pharmacotherapy of stable angina pectoris implies an in-depth study of the pharmacogenetic factor when prescribing the recommended drugs in order to increase the effectiveness and safety of their use. The work is devoted to the development of individual approaches to the appointment of meldonium in the complex pharmacotherapy of angina pectoris as a cardiac cytoprotector with endothelioprotective properties, depending on gene polymorphism of endothelial nitric oxide synthase. Research tasks: To study the polymorphism of the endothelial nitric oxide synthase gene eNOS C786T and to evaluate the significance of this factor in the manifestation of the genoprotective properties of meldonium in patients with stable angina pectoris. Methods: A total of 90 patients with stable angina pectoris were examined. DNA was isolated from blood leukocytes from patients. The polymorphism of endothelial nitric oxide synthase eNOS C786T was determined by polymerase chain reaction. By the method of DNA comets according to the method developed by us in in vitro tests, the effect of meldonium on the DNA of blood leukocytes of patients was evaluated. The content of cortisol, cyclic AMP and cyclic GMP in the blood serum of patients, concentration of endothelial and inducible nitric oxide synthases in erythrocytes was determined by the enzyme immunoassay. Results: The presence of gene polymorphisms of eNOS C786T alleles was found. They revealed a more severe clinical condition of patients having pathological genes by eNOS C786T alleles in the form of signs of a decrease in the activity of stress-limiting and increasing activity of stress-realizing systems at the level of humoral regulation and intracellular messengers in connection with the apparent decrease in the enzymatic activity of endothelial nitric oxide synthase. Potential genotoxicity of meldonium was revealed when it was administered to patients with a pathological genotype of endothelial nitric oxide synthase eNOS C786T (CC, CT) and a genoprotective effect when administered to patients with a normal genotype of endothelial nitric oxide synthase eNOS C786T (TT). Conclusion: When prescribing meldonium to patients with stable angina, one should take into account the genetic information on the individual polymorphism of the endothelial nitric oxide synthase gene and give preference to individuals who have the normal genotype of eNOS C786T (TT) in view of the predicted genoprotective effect of the drug. Key words: pharmacogenetics, polymorphism of the gene eNOS C786T, meldonium, genotoxicity, genoprotection, personalized pharmacotherap