Effect of ELF e.m. fields on metalloprotein redox-active sites

The peculiarity of the distribution and geometry of metallic ions in enzymes pushed us to set the hypothesis that metallic ions in active-site act like tiny antennas able to pick up very feeble e.m. signals. Enzymatic activity of Cu2+, Zn2+ Superoxide Dismutase (SOD1) and Fe2+ Xanthine Oxidase (XO) has been studied, following in vitro generation and removal of free radicals. We observed that Superoxide radicals generation by XO is increased by a weak field having the Larmor frequency fL of Fe2+ while the SOD1 kinetics is sensibly reduced by exposure to a weak field having the frequency fL of Cu2+ ion.Comment: 18 pages, 4 figure

CEH-GEAR: 1 km resolution daily and monthly areal rainfall estimates for the UK for hydrological and other applications

The Centre for Ecology & Hydrology – Gridded Estimates of Areal Rainfall (CEH-GEAR) data set was developed to provide reliable 1 km gridded estimates of daily and monthly rainfall for Great Britain (GB) and Northern Ireland (NI) (together with approximately 3500 km2 of catchment in the Republic of Ireland) from 1890 onwards. The data set was primarily required to support hydrological modelling. The rainfall estimates are derived from the Met Office collated historical weather observations for the UK which include a national database of rain gauge observations. The natural neighbour interpolation methodology, including a normalisation step based on average annual rainfall (AAR), was used to generate the daily and monthly rainfall grids. To derive the monthly estimates, rainfall totals from monthly and daily (when complete month available) rain gauges were used in order to obtain maximum information from the rain gauge network. The daily grids were adjusted so that the monthly grids are fully consistent with the daily grids. The CEH-GEAR data set was developed according to the guidance provided by the British Standards Institution. The CEH-GEAR data set contains 1 km grids of daily and monthly rainfall estimates for GB and NI for the period 1890–2012. For each day and month, CEH-GEAR includes a secondary grid of distance to the nearest operational rain gauge. This may be used as an indicator of the quality of the estimates. When this distance is greater than 100 km, the estimates are not calculated due to high uncertainty

Making better use of local data in flood frequency estimation

Flood frequency estimates are an essential part of flood risk management. They are an important ingredient of many important decisions, informing the cost-effectiveness, design and operation of flood defences, flood mapping and planning decisions in flood risk areas. They also inform the National Flood Risk Assessment, the setting of insurance premiums and long-term investment planning. Methods described in the Flood Estimation Handbook (FEH) published in 1999, and many subsequent updates, are considered the industry standard for flood estimation in the UK. They are used extensively by hydrologists from both the public and private sectors. Flood frequency estimates – also known as design flood estimates – are associated with many sources of uncertainty. These hydrological uncertainties often constitute the most uncertain component in any flood study. Uncertainty can lead to difficulty in having confidence in the outputs of studies, whether these are for investment planning, insurance, asset design, development planning or other purposes. As a result, there is considerable benefit to be gained from any reduction in the uncertainty of flood frequency estimation. There are many supplementary sources of information that can help to refine estimates of design floods and potentially reduce uncertainty. Examples include long-term flood history, river level records, photographs of floods and information obtained from field visits. These and similar types of information are defined as ‘local data’. The FEH Local research project aimed to: quantify the uncertainty of design floods estimated from FEH methods develop procedures and guidance for incorporating local and historical data into flood estimation to reduce uncertainties The primary objective of this report is to describe the reviews and research carried out during the FEH Local project. Another output from the project was a document giving guidance to practitioners on how to estimate uncertainty in flood frequency and how to find and incorporate local data. The practitioner guidance, ‘Using Local Data to Reduce Uncertainty in Flood Frequency Estimation’, will be disseminated early in 2017. This report aims to avoid duplication with the practitioner guidance and so is intended mainly for those with an interest in the background to the methods presented in the guidance

Going Beyond the Ensemble Mean: Assessment of Future Floods From Global Multi‐Models

Future changes in the occurrence of flood events can be estimated using multi-model ensembles to inform adaption and mitigation strategies. In the near future, these estimates could be used to guide the updating of exceedance probabilities for flood control design and water resources management. However, the estimate of return levels from ensemble experiments represents a challenge: model runs are affected by biases and uncertainties and by inconsistencies in simulated peak flows when compared with observed data. Moreover, extreme value distributions are generally fit to ensemble members individually and then averaged to obtain the ensemble fit with loss of information. To overcome these limitations, we propose a Bayesian hierarchical model for assessing changes in future peak flows, and the uncertainty coming from global climate, global impact models and their interaction. The model we propose allows use of all members of the ensemble at once for estimating changes in the parameters of an extreme value distribution from historical to future peak flows. The approach is applied to a set of grid-cells in the eastern United States to the full and to a constrained version of the ensemble. We find that, while the dominant source of uncertainty in the changes varies across the domain, there is a consensus on a decrease in flood magnitudes toward the south. We conclude that projecting future flood magnitude under climate change remains elusive due to large uncertainty mostly coming from global models and from the intrinsic uncertain nature of extreme values

A pricing formula for delayed claims: appreciating the past to value the future

We consider the valuation of contingent claims with delayed dynamics in a Samuelson complete market model. We find a pricing formula that can be decomposed into terms reflecting the current market values of the past and the future, showing how the valuation of prospective cashflows cannot abstract away from the contribution of the past. As a practical application, we provide an explicit expression for the market value of human capital in a setting with wage rigidity. The formula we derive has successfully been used to explicitly solve the infinite dimensional stochastic control problems addressed in [7], [6] and [16]

Evolutionary relationships among barley and <i>Arabidopsis</i> core circadian clock and clock-associated genes

The circadian clock regulates a multitude of plant developmental and metabolic processes. In crop species, it contributes significantly to plant performance and productivity and to the adaptation and geographical range over which crops can be grown. To understand the clock in barley and how it relates to the components in the Arabidopsis thaliana clock, we have performed a systematic analysis of core circadian clock and clock-associated genes in barley, Arabidopsis and another eight species including tomato, potato, a range of monocotyledonous species and the moss, Physcomitrella patens. We have identified orthologues and paralogues of Arabidopsis genes which are conserved in all species, monocot/dicot differences, species-specific differences and variation in gene copy number (e.g. gene duplications among the various species). We propose that the common ancestor of barley and Arabidopsis had two-thirds of the key clock components identified in Arabidopsis prior to the separation of the monocot/dicot groups. After this separation, multiple independent gene duplication events took place in both monocot and dicot ancestors. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00239-015-9665-0) contains supplementary material, which is available to authorized users

Pan-Atlantic analysis of the overlap of a highly migratory species, the leatherback turtle, with pelagic longline fisheries

This is the final version of the article. Available from the publisher via the DOI in this record.Large oceanic migrants play important roles in ecosystems, yet many species are of conservation concern as a result of anthropogenic threats, of which incidental capture by fisheries is frequently identified. The last large populations of the leatherback turtle, Dermochelys coriacea, occur in the Atlantic Ocean, but interactions with industrial fisheries could jeopardize recent positive population trends, making bycatch mitigation a priority. Here, we perform the first pan-Atlantic analysis of spatio-temporal distribution of the leatherback turtle and ascertain overlap with longline fishing effort. Data suggest that the Atlantic probably consists of two regional management units: northern and southern (the latter including turtles breeding in South Africa). Although turtles and fisheries show highly diverse distributions, we highlight nine areas of high susceptibility to potential bycatch (four in the northern Atlantic and five in the southern/equatorial Atlantic) that are worthy of further targeted investigation and mitigation. These are reinforced by reports of leatherback bycatch at eight of these sites. International collaborative efforts are needed, especially from nations hosting regions where susceptibility to bycatch is likely to be high within their exclusive economic zone (northern Atlantic: Cape Verde, Gambia, Guinea Bissau, Mauritania, Senegal, Spain, USA and Western Sahara; southern Atlantic: Angola, Brazil, Namibia and UK) and from nations fishing in these high-susceptibility areas, including those located in international waters.Work in Gabon was financially supported by the Large Pelagics Research Center through National Oceanographic and Atmospheric Agency award no. NA04NMF4550391, the UK Defra Darwin Initiative, the Shellshock Campaign (European Association for Zoos and Aquaria) and the UK Natural Environment Research Council. Sea turtle monitoring programmes in Gabon were financially supported by the Wildlife Conservation Society and by the Gabon Sea Turtle Partnership with funding from the Marine Turtle Conservation Fund (United States Fish and Wildlife Service, US Department of the Interior). Four of the satellite tags were deployed in Canadian waters by M. James (Dalhousie University) and the Canadian Sea Turtle Network, with the funding support of Canadian Sea Turtle Network leatherback field research provided by R. A. Myers, the Canadian Wildlife Federation, Environment Canada and WWF-Canada. Work in French Guiana was financially supported by CNES, DEAL and the European Union.This study results from the collaborative effort of 10 data providers, which have satellite-tracked leatherback turtles in the Atlantic Ocean since 1995, through their voluntary participation in the Trans-Atlantic Leatherback Conservation Initiative (TALCIN), a WWF-led initiative. We thank C. Drews (WWF-International) and Jean-Yves Georges (IPHC-CNRS) for having initiated this project. Significant contributions were made by A. Fonseca and M. L. Felix and the WWF Guianas office in fostering this project to secure its continuation. We thank those involved in the sea turtle restoration plan in French Guiana (DEAL, ONCFS, Kulalasi NGO, Kwata, the Reserve Naturelle de l'Amana, Chiefs of Awala and Yalimapo), Yvon Le Maho (IPHC-CNRS) for having initiated the leatherback tracking programme in French Guiana, colleagues from the Regional Program for Sea Turtles Research and Conservation of Argentina–PRICTMA, Aquamarina and Fundación Mundo Marino, the onboard scientific observers from PNOFA-DINARA, the crew and owner of the F/V Torres del Paine, the artisanal fishermen from Kiyú, San José, Uruguay, D. del Bene (PROFAUMA), Z. Di Rienzo and colleagues from Karumbé, the University of Pisa for initiating the satellite tagging programmes in South Africa, and the South African Department of Environmental Affairs for continuing the work in cooperation with Dr Nel from the Nelson Mandela Metropolitan University, Port Elizabeth and Ezemvelo KZN Wildlife. We thank M. L. Felix for her efforts in the deployment of satellite tags in Suriname and the Nature Conservation Division Suriname for facilitating these research efforts. P.M. thanks C. Palma for his help in dealing with ICCAT's database, C. Ere, as well as the GIS training and support received from SCGIS and the ESRI Conservation Program, which allowed processing of fishing-effort data. We thank J. Parezo for her careful reading of the manuscript. All authors designed the study and contributed data; S.F, M.S.C., P.M. and M.J.W. compiled the data; S.F., M.A.N. and A.L. coordinated and supervised the project; S.F., M.J.W., P.M. and B.J.G. led the data analysis and interpretation with contributions from all authors; the manuscript was developed by S.F. and M.J.W. as lead authors, with contributions from all authors

An investigation of grand battement devant at barre, centre, and in motion using kinematics and electromyography

The purpose of this study was to examine grand battement devant in three conditions: at the barre, in the centre, and traveling. The primary focus was to consider weight transfer in the three conditions, and to examine utilisation of the trunk and lower extremity muscles. An extensive review was done in the dance science literature to determine what previous research had been done related to this subject, and to establish what preliminary work might be needed. As indicated by the literature, in order to achieve this research, it was necessary to develop a dance-specific method for the normalisation of surface electromyography data. In phase one of the research, a dance-specific portable anchored dynamometer was developed and tested. The PAD allowed for the collection of maximum voluntary isometric contractions (MVICs), which could then be used to normalise the sEMG data. In phase two of the research, the grand battement was tested in the three conditions, at the barre, in the centre, and traveling. Forty female dancers volunteered (mean age 30.0 ± 13.0 yrs, mean height 1.63 ± 0.06 m, mean mass 59.0 ± 7.4 kg, and 13.9 ± 13.3 yrs of training in ballet and/or modern dance) and were placed in three groups (Training level): beginner (n = 12), intermediate (n = 14) and advanced (n = 14). Dancers executed five grand battement devant in each of the three conditions (Condition) in randomized order. Data were collected with a 7-camera Vicon motion capture system, two Kistler forceplates, and surface electromyography (EMG), using eight muscles bilaterally. Kinematic data were analysed in three intervals: stance to battement initiation, initiation to battement peak, and peak to end. Four variables were investigated: centre of gravity of the full trunk, centre of gravity of the pelvis, centre of gravity of the upper trunk, and centre of mass. EMG data were analysed in four events: stance, initiation, peak, and end. For weight transfer, the main effect of Condition was significant for all four variables in both the x-axis and the y-axis (p < .001). There were no significant differences for Training and no significant Condition x Training interactions. Muscle use varied according to the combination of event and condition that was executed, and these differences were also influenced by the level of training of the dancer and the side of the body used. It is recommended that dance educators consider the importance of allocating sufficient time to each of the three conditions (barre, centre, and traveling), to ensure development of a variety of motor strategies and muscle activation patterns for dance practice.EThOS - Electronic Theses Online ServiceGBUnited Kingdo

The physiological responses of cacao to the environment and the implications for climate change resilience. A review

Cacao (Theobroma cacao L.) is a tropical perennial crop which is of great economic importance to the confectionary industry and to the economies of many countries of the humid tropics where it is grown. Some recent studies have suggested climate change could severely impact cacao production in West Africa. It is essential to incorporate our understanding of the physiology and genetic variation within cacao germplasm when discussing the implications of climate change on cacao productivity and developing strategies for climate resilience in cacao production. Here we review the current research on the physiological responses of cacao to various climate factors. Our main findings are 1) water limitation causes significant yield reduction in cacao but genotypic variation in sensitivity is evident, 2) in the field cacao experiences higher temperatures than is often reported in the literature, 3) the complexity of the cacao/ shade tree interaction can lead to contradictory results, 4) elevated CO2 may alleviate some negative effects of climate change 5) implementation of mitigation strategies can help reduce environmental stress, 6) significant gaps in the research need addressing to accelerate the development of climate resilience. Harnessing the significant genetic variation apparent within cacao germplasm is essential to develop modern varieties capable of high yields in non-optimal conditions. Mitigation strategies will also be essential but to use shading to best effect shade tree selection is crucial to avoid resource competition. Cacao is often described as being sensitive to climate change but genetic variation, adaptive responses, appropriate mitigation strategies and interactive climate effects should all be considered when predicting the future of cacao production. Incorporating these physiological responses to various environmental conditions and developing a deeper understanding of the processes underlying these responses will help to accelerate the development of a more resource use efficient tree ensuring sustainable production into the future