The systematic position of Plagiochila moritziana, P. trichostoma and P. deflexa based on ITS sequence variation of nuclear ribosomal DNA, morphology, and lipophilic secondary metabolites

According to phylogenetic analyses of nrDNA ITS1 and ITS2 sequences (including the 5.8S unit) the Neotropical Plagiochila moritziana, P. rutilans var. rutilans, P. rutilans var. standleyi, P. trichostoma (= P. permista, syn. nov.), and P. subtrinitensis form a monophyletic lineage and are placed in P. sect. Rutilantes; all five taxa lack a ca 20 base pair sequence that is present in all the taxa of the other Plagiochila sections investigated. The Central American P. subtrinitensis is treated as a synonym of the Hawaiian endemic P. deflexa. Plagiochila moritziana is excluded from sect. Fuscoluteae and reduced to a variety of P. rutilans; P. sect. Permistae is treated as a synonym of P. sect. Rutilantes. The sporophytes of P. trichostoma and P. deflexa are described for the first time. Fresh material of P. rutilans var. moritziana exhibits a distinct odor of peppermint caused by the presence of several menthane monoterpenoids, principally pulegone. The Central American P. rutilans var. standleyi is reported from Ecuador, new to South America. Lectotypes are designated for P. rutilans var. moritziana, P. subtrinitensis, and P. trichostoma

Plagiochila virginica A. Evans rather than P-dubia Lindenb. and Gottsche occurs in Macaronesia; placement in sect. Contiguae Carl is supported by ITS sequences of nuclear ribosomal DNA

Plagiochila dubia Lindenb. and Gottsche is reduced to a synonym of the Neotropical P. panda (Sw.) Lindenb. Specimens from the Canary Islands and Madeira proved to belong to the eastern North American P. virginica A.Evans, new to Europe. Phylogenetic analyses of the internal transcribed spacer regions (ITS1-5.8S-ITS2) of nuclear ribosomal DNA of ten Plagiochila species produced for independent lineages that are well supported by all bootstrap analyses (maximum likelihood, maximum parsimony, and distance). These lineages correspond with the Plagiochila sections Arrectae, Contiguae, Cucullatae and Glaucescentes. Spruce's "Ramiflorae" and "Cauliflorae" may no longer be regarded as monophyletic units of Plagiochila

Phylogenetic relationship and divergence among planktonic strains of Arthrospira (Oscillatoriales, Cyanobacteria) of African, Asian and American origin deduced by 16S-23S ITS and phycocyanin operon sequences

Arthrospira comprises multicellular, cylindrical, usually screwlike coiled trichomes and is cultivated commercially. In this study, 33 new strains of Arthrospira isolated from plankton samples collected in Mexico, East Africa and India were investigated and compared with 53 strains or samples of earlier considerations. The study included observations of morphological features and molecular phylogenetic analyses on the basis of nucleotide sequences of internal transcribed spacer (ITS) between 16S rRNA and 23S rRNA genes and partial sequences of beta and alpha subunits including intergenic spacer (cpcBA-IGS) of phycocyanin operon. Morphological traits of Arthrospira such as trichome width, type of coiling and apical cell were not always consistent in culture conditions. It was revealed that Arthrospira phylogeny on the basis of cpcBA-IGS locus was broadly comparable with the ITS region as both phylogenetic trees derived from nucleotide sequences could be divided into two main clusters. Cluster I comprised sequences from American strains mainly, whereas cluster II contained the sequences of the strains originating from Africa and Asia chiefly. Both genetic regions of the strains investigated in the present study coincidently showed a significant sequence divergence among Arthrospira strains from East Africa, India and Mexico indicating possible distinct evolutionary lineages