Uncle traps: harvesting rewards in a queue-based ethereum Mining Pool

Mining pools in Proof-of-Work cryptocurrencies allow miners topool their computational resources as a means of reducing payoutvariance. In Ethereum,uncle blocksare valid Proof-of-Work solu-tions which do not become the head of the blockchain, yet yieldrewards if later referenced by main chain blocks. Mining pool opera-tors are faced with the non-trivial task of fairly distributing rewardsfor both block types among pool participants.Inspired by empirical observations, we formally reconstruct aSybil attack exploiting the uncle block distribution policy in a queue-based mining pool. To ensure fairness of the queue-based payoutscheme, we propose a mitigation. We examine the effectiveness ofthe attack strategy under the current and the proposed policy via adiscrete-event simulation. Our findings show that the observed attackcan indeed be obviated by altering the current reward scheme

Protein-Protein Fusion Catalyzed by Sortase A

Chimeric proteins boast widespread use in areas ranging from cell biology to drug delivery. Post-translational protein fusion using the bacterial transpeptidase sortase A provides an attractive alternative when traditional gene fusion fails. We describe use of this enzyme for in vitro protein ligation and report the successful fusion of 10 pairs of protein domains with preserved functionality — demonstrating the robust and facile nature of this reaction

Evaluation of ELISA and haemagglutination inhibition as screening tests in serosurveillance for H5/H7 avian influenza in commercial chicken flocks

Avian influenza virus (AIV) subtypes H5 and H7 can infect poultry causing low pathogenicity (LP) AI, but these LPAIVs may mutate to highly pathogenic AIV in chickens or turkeys causing high mortality, hence H5/H7 subtypes demand statutory intervention. Serological surveillance in the European Union provides evidence of H5/H7 AIV exposure in apparently healthy poultry. To identify the most sensitive screening method as the first step in an algorithm to provide evidence of H5/H7 AIV infection, the standard approach of H5/H7 antibody testing by haemagglutination inhibition (HI) was compared with an ELISA, which detects antibodies to all subtypes. Sera (n = 1055) from 74 commercial chicken flocks were tested by both methods. A Bayesian approach served to estimate diagnostic test sensitivities and specificities, without assuming any 'gold standard'. Sensitivity and specificity of the ELISA was 97% and 99.8%, and for H5/H7 HI 43% and 99.8%, respectively, although H5/H7 HI sensitivity varied considerably between infected flocks. ELISA therefore provides superior sensitivity for the screening of chicken flocks as part of an algorithm, which subsequently utilises H5/H7 HI to identify infection by these two subtypes. With the calculated sensitivity and specificity, testing nine sera per flock is sufficient to detect a flock seroprevalence of 30% with 95% probability

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The following report provides results from a geochemical survey for Thomas Range-Wasatch, Utah. Field and laboratory data are presented for 15 groundwater and 79 stream sediment samples

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Documentation outlining characteristics of field data samples taken in the Buffalo-Lignite detail area

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Results of the Chinati Mountains project area of the detailed geochemical survey fro Trans-Pecos, Texas are reported

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Results of the Dryden project area of the detailed geochemical survey for Trans-Pecos, Texas are reported

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Results of the Stillwell Mountains project area of the detailed geochemical survey for Trans-Pecos, Texas are reported

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Documentation outlining characteristics of field data samples taken in the Stillwell Mountains project area

Site-specific protein modification using immobilized sortase in batch and continuous-flow systems

Transpeptidation catalyzed by ​sortase A allows the preparation of proteins that are site-specifically and homogeneously modified with a wide variety of functional groups, such as fluorophores, PEG moieties, lipids, glycans, bio-orthogonal reactive groups and affinity handles. This protocol describes immobilization of ​sortase A on a solid support (Sepharose beads). Immobilization of ​sortase A simplifies downstream purification of a protein of interest after labeling of its N or C terminus. Smaller batch and larger-scale continuous-flow reactions require only a limited amount of enzyme. The immobilized enzyme can be reused for multiple cycles of protein modification reactions. The described protocol also works with a Ca²⁺-independent variant of ​sortase A with increased catalytic activity. This heptamutant variant of ​sortase A (7M) was generated by combining previously published mutations, and this immobilized enzyme can be used for the modification of calcium-senstive substrates or in instances in which low temperatures are needed. Preparation of immobilized ​sortase A takes 1–2 d. Batch reactions take 3–12 h and flow reactions proceed at 0.5 ml h⁻¹, depending on the geometry of the reactor used.United States. National Institutes of Health (RO1 AI087879