The prefusion structure of herpes simplex virus glycoprotein B.

Cell entry of enveloped viruses requires specialized viral proteins that mediate fusion with the host membrane by substantial structural rearrangements from a metastable pre- to a stable postfusion conformation. This metastability renders the herpes simplex virus 1 (HSV-1) fusion glycoprotein B (gB) highly unstable such that it readily converts into the postfusion form, thereby precluding structural elucidation of the pharmacologically relevant prefusion conformation. By identification of conserved sequence signatures and molecular dynamics simulations, we devised a mutation that stabilized this form. Functionally locking gB allowed the structural determination of its membrane-embedded prefusion conformation at sub-nanometer resolution and enabled the unambiguous fit of all ectodomains. The resulting pseudo-atomic model reveals a notable conservation of conformational domain rearrangements during fusion between HSV-1 gB and the vesicular stomatitis virus glycoprotein G, despite their very distant phylogeny. In combination with our comparative sequence-structure analysis, these findings suggest common fusogenic domain rearrangements in all class III viral fusion proteins

The pre-fusion structure of Herpes simplex virus glycoprotein B

Cell entry of enveloped viruses requires specialized viral proteins which mediate fusion with the host membrane by substantial structural rearrangements from a metastable pre- to a stable postfusion conformation. This metastability renders the Herpes simplex virus (HSV-1) fusion glycoprotein B (gB) highly unstable such that it readily converts into the post-fusion form, thereby precluding structural elucidation of the pharmacologically relevant pre-fusion conformation. By identification of conserved sequence signatures and molecular dynamics simulations, we devised a mutation that stabilized this form. Functionally locking gB, allowed the structural determination of its membrane-embedded pre-fusion conformation at sub-nanometer resolution and enabled the unambiguous fit of all ectodomains. The resulting pseudo-atomic model reveals a striking conservation of conformational domain rearrangements during fusion between HSV-1 gB and the Vesicular Stomatitis Virus glycoprotein G (VSV-G) despite their very distant phylogeny. In combination with our comparative sequence-structure analysis, these findings suggest common fusogenic domain rearrangements in all class III viral fusion proteins. Rey, M. Topf, K

Modeling, Evaluation, and Scale on Artificial Pedestrians: A Literature Review

Modeling pedestrian dynamics and their implementation in a computer are challenging and important issues in the knowledge areas of transportation and computer simulation. The aim of this article is to provide a bibliographic outlook so that the reader may have quick access to the most relevant works related to this problem. We have used three main axes to organize the article's contents: pedestrian models, validation techniques, and multiscale approaches. The backbone of this work is the classification of existing pedestrian models; we have organized the works in the literature under five categories, according to the techniques used for implementing the operational level in each pedestrian model. Then the main existing validation methods, oriented to evaluate the behavioral quality of the simulation systems, are reviewed. Furthermore, we review the key issues that arise when facing multiscale pedestrian modeling, where we first focus on the behavioral scale (combinations of micro and macro pedestrian models) and second on the scale size (from individuals to crowds). The article begins by introducing the main characteristics of walking dynamics and its analysis tools and concludes with a discussion about the contributions that different knowledge fields can make in the near future to this exciting area

EVALITA Evaluation of NLP and Speech Tools for Italian - December 17th, 2020

Welcome to EVALITA 2020! EVALITA is the evaluation campaign of Natural Language Processing and Speech Tools for Italian. EVALITA is an initiative of the Italian Association for Computational Linguistics (AILC, http://www.ai-lc.it) and it is endorsed by the Italian Association for Artificial Intelligence (AIxIA, http://www.aixia.it) and the Italian Association for Speech Sciences (AISV, http://www.aisv.it)

Robustní metodologie hodnocení vylepšení systému TTS

Článek ukazuje na problematické a obvykle zanedbávané aspekty evaluace TTS systémů poslechovými testy. Je ukázáno, že obyčejný náhodný výběr frází k poslechu nemusí pokrýt případy, které jsou relevantní vzhledem k hodnocenému systému TTS. Je také ukázáno, že bez hlubší znalosti distribuce rozdílů v syntetické řeči, získaných porovnáním výstupů základního a hodnoceného systému, nelze vybrat spolehlivou množina frází k evaluaci. Předkládáme tedy, a na skutečných případech ukazujeme metodu, která v případě, že známe distribuci rozdílů, je schopna vyčíslit míru spolehlivosti poslechového testu stejně jako odhadnou pravděpodobnost neplatnosti závěrů získaných z výsledků poslechového testu.The paper points to problematic and usually neglected aspects of using listening tests for TTS evaluation. It shows that simple random selection of phrases to be listened to may not cover those cases which are relevant to the evaluated TTS system. Also, it shows that a reliable phrase set cannot be chosen without a deeper knowledge of the distribution of differences in synthetic speech, which are obtained by comparing the output generated by an evaluated TTS system to what stands as a baseline system. Having such knowledge, the method able to evaluate the reliability of listening tests, as related to the estimation of possible invalidity of listening results-derived conclusion, is proposed here and demonstrated on real examples

Moving contact line of a volatile fluid

International audienceInterfacial flows close to a moving contact line are inherently multiscale. The shape of the interface and theflow at meso- and macroscopic scales inherit an apparent interface slope and a regularization length, both namedafter Voinov, from the microscopic inner region. Here, we solve the inner problem associated with the contact linemotion for a volatile fluid at equilibrium with its vapor. The evaporation or condensation flux is then controlled bythe dependence of the saturation temperature on interface curvature—the so-called Kelvin effect. We derive thedependencies of the Voinov angle and of the Voinov length as functions of the parameters of the problem. We thenidentify the conditions under which the Kelvin effect is indeed the mechanism regularizing the contact line motion