Evaluating the Vulnerability of Mountain Springs: A Case Study in Italy to Prioritize Conservation and Management Strategies

This research introduces a methodology for evaluating the protection zone of vulnerable mountain springs using an hydrogeochemical approach. Mountain springs play a crucial role in maintaining the ecological balance and ensuring the well-being and resilience of communities residing in mountainous areas. These resources frequently serve as the primary freshwater supply in numerous mountainous regions, their impact extends beyond these areas by catering to diverse applications, including agriculture, farming, hydropower generation, artificial snowmaking, and industrial utilization. Despite their importance, mountain springs are under increasing threat due to climate change and human activities and thus need to be preserved and managed to ensure a sustainable use and conservation. In this study, we assess the vulnerability of two mountain springs located in a karstic water system in the Northern Italy mountainous region. Particularly we analyze the hydrogeological and hydrogeochemical parameters of the two mountain springs, together with the oxygen and hydrogen isotopic composition (δ18O and δ2H) and d-excess of both the springs and the rainwater of the area. The considered parameters were continuously measured from September 2018 to September 2021. The main goal is to assess the geochemical and hydrological processes that control the springs water quality and the isotopic composition of precipitation and use them for formulating effective springs protection measures. Our results show that the vulnerability of mountain springs is influenced by various factors that include the use of the resource, the meteorological conditions, and the hydrogeology of the area. We propose a method that integrates the Vulnerability Estimator for Spring Protection Areas index with the use of the water stable isotopes to identify springs’ protection zones that takes in consideration the recharge area of the aquifers feeding the springs. Our study contributes to the development of a framework for assessing the vulnerability of mountain springs and highlights the importance of integrating the geochemical characteristics and the anthropic pressure in the conservation and management of these critical freshwater resources. This study is part of Next Innovation Ecosystem Program "Interconnected Northeast Innovation Ecosystem (iNEST)" supported by the European Union

Multidimensional prognostic index and mortality in intermediate care facilities: A retrospective study

Multidimensional prognostic index (MPI) is a frailty assessment tool used for stratifying prognosis in older hospitalized people, but data regarding older people admitted to intermediate care facilities (ICFs) are missing. The aim of this study is to evaluate whether MPI can predict mortality in older patients admitted to the ICFs. MPI was calculated using different domains explored by a standard comprehensive geriatric assessment and categorized into tertiles (MPI-1 ≤ 0.20, MPI 2 0.20–0.34, MPI 3 > 0.34). A Cox’s regression analysis, taking mortality as the outcome, was used, reporting the results as hazard ratios (HRs) with 95% confidence intervals (CIs). In total, 653 older patients were enrolled (mean age: 82 years, 59.1% females). Patients in MPI-2 (HR = 3.66; 95%CI: 2.45–5.47) and MPI-3 (HR = 6.22; 95%CI: 4.22–9.16) experienced a higher risk of mortality, compared to MPI-1. The accuracy of MPI in predicting mortality was good (area under the curve (AUC) = 0.74, 95%CI: 0.70–0.78). In conclusion, our study showed that prognostic stratification, as assessed by the MPI, was associated with a significantly different risk of mortality in older patients admitted to the ICFs, indicating the necessity of using a CGA-based tool for better managing older people in this setting as well. © 2021 by the authors. Licensee MDPI, Basel, Switzerland

Evidence of sexuality in induced tetraploids of Brachiaria brizantha (Poaceae).

Made available in DSpace on 2018-05-31T00:36:58Z (GMT). No. of bitstreams: 1 ID262371.pdf: 517213 bytes, checksum: 2243f8787a54feb71a540022fff8de45 (MD5) Previous issue date: 2005-12-1

Aspectos econômico-produtivos da atividade leiteira em sistemas de produção de base familiar na região noroeste do Rio Grande do Sul.

A Região Noroeste do Rio Grande do Sul representa um importante pólo de produção leiteira e, a qual está, em grande parte, baseada em unidades familiares. Assim, o objetivo deste trabalho foi caracterizar, analisar e discutir aspectos econômico-produtivos desses sistemas de produção, com enfoque na pecuária leiteira. Interagindo com as famílias de agricultores participantes do Programa Rede Leite, tem-se acompanhado várias unidades produtivas, observando e realizando registros. Nesse estudo, analisaram-se dados provenientes de 29 propriedades, caracterizadas por 18 indicadores econômico-produtivos, utilizando-se como ferramenta a estatística multivariada. Identificou-se a formação de dois tipos básicos de sistemas de produção, diferenciados principalmente pela área total da propriedade, que, por sua vez, condiciona diferenças em outras variáveis. Os agricultores dispõem de variadas estratégias produtivas em função das condições e recursos próprios do sistema. A identificação e caracterização dos tipos de sistemas de produção nos permitem gerar proposições no sentido de melhorar os processos produtivos e conferir maior sustentabilidade

Mortality attributable to COVID-19 in nursing home residents: a retrospective study

Aim: Coronavirus-19 disease (COVID-19) is a widespread condition in nursing home (NH). It is not known whether COVID-19 is associated with a higher risk of death than residents without COVID-19. Therefore, the aim of this study was to assess whether COVID-19 is associated with a higher mortality rate in NH residents, considering frailty status assessed with the Multidimensional Prognostic Index (MPI). Methods: In this retrospective study, made in 31 NHs in Venice, Italy, the presence of COVID-19 was ascertained with a nasopharyngeal swab. Frailty was evaluated using the MPI, modified according to the tools commonly used in our NHs. A Cox’s regression analysis was used reporting the results as hazard ratios (HRs) with 95% confidence intervals (CIs), using COVID-19 as exposure and mortality as outcome and stratified by MPI tertiles. Similar analyses were run using MPI tertiles as exposure. Results: Overall, 3946 NH residents (median age = 87 years, females: 73.9%) were eligible, with 1136 COVID-19 +. During a median follow-up of 275 days, higher values of MPI, indicating frailer people, were associated with an increased risk of mortality. The incidence of mortality in COVID-19 + was more than doubled than COVID-19- either in MPI-1, MPI-2 and MPI-3 groups. The presence of COVID-19 increased the risk of death (HR = 1.85; 95% CI 1.59–2.15), also in the propensity score model using MPI as confounder (HR = 2.48; 95% CI 2.10–2.93). Conclusion: In this retrospective study of NH residents, COVID-19 was associated with a higher risk of all-cause mortality than those not affected by COVID-19 also considering the different grades of frailty. © 2021, The Author(s)

Técnica de clareamento modificada na análise de sacos embrionários em Brachiaria e Paspalum (gramineae) através da microscopia interferencial.

A aplicação de técnicas citológicas e histológicas nas análises de rotina feita com espécimens, incluídos em parafina, é extremamente demorada e laboriosa, enquanto que a técnica de clareamento, associada a microscopia interferencial, apresenta rapidez e eficiência, principalmente nos estudos envolvendo grande quantidade de amostras. Esta técnica é amplamente utilizada em plantas, principalmente em estudos do sistema vascular foliar e de flores, auxiliando neste último, a determinação do tipo de saco embrionário presente nos ovários. Isto permite de forma rápida, correlacionar o tipo destas estruturas ao modo de reprodução de diferentes espécies de plantas, incluíndo gramíneas.bitstream/item/90710/1/2001.pd

Dysfunctional mitochondria accumulate in a skeletal muscle knockout model of Smn1, the causal gene of spinal muscular atrophy

The approved gene therapies for spinal muscular atrophy (SMA), caused by loss of survival motor neuron 1 (SMN1), greatly ameliorate SMA natural history but are not curative. These therapies primarily target motor neurons, but SMN1 loss has detrimental effects beyond motor neurons and especially in muscle. Here we show that SMN loss in mouse skeletal muscle leads to accumulation of dysfunctional mitochondria. Expression profiling of single myofibers from a muscle specific Smn1 knockout mouse model revealed down-regulation of mitochondrial and lysosomal genes. Albeit levels of proteins that mark mitochondria for mitophagy were increased, morphologically deranged mitochondria with impaired complex I and IV activity and respiration and that produced excess reactive oxygen species accumulated in Smn1 knockout muscles, because of the lysosomal dysfunction highlighted by the transcriptional profiling. Amniotic fluid stem cells transplantation that corrects the SMN knockout mouse myopathic phenotype restored mitochondrial morphology and expression of mitochondrial genes. Thus, targeting muscle mitochondrial dysfunction in SMA may complement the current gene therapy.We thank Drs F. Caicci and F. Boldrin (EM Facility, Department of Biology, University of Padova) for electron microscopy samples preparation; Dr. C. Millino and Dr. B. Pacchioni (Microarray Service MicroCribi, University of Padova) for the help with microarray experiments; S. Schiavon and Dr. G. Covello (HiTS@uniPD Facility, Department of Biology, University of Padova) for High Content Imaging; Foundation Umberto Veronesi for supporting TV; Dr. R. Cerutti for help with histochemical assay; and Prof. I. Szabo for providing reagents. This work was supported by AFM-Telethon2013/Project 16662 (to CB); and by CARIPARO Project 13/04 and 27/01 (to MP). This work contributes to the COST Action CA17116 “International Network for Translating Research on Perinatal Derivatives into Therapeutic Approaches” (SPRINT; to MP)

Combined Therapy with Insulin and Growth Hormone in 17 Patients with Type-1 Diabetes and Growth Disorders.

Combined growth hormone (GH) and insulin therapy is rarely prescribed by pediatric endocrinologists. We investigated the attitude of Italian physicians to prescribing that therapy in the case of short stature and type-1 diabetes (T1DM). Methods: A questionnaire was sent and if a patient was identified, data on growth and diabetes management were collected. Results: Data from 42 centers (84%) were obtained. Of these, 29 centers reported that the use of combined therapy was usually avoided. A total of 17 patients were treated in 13 centers (GH was started before T1DM onset in 9 patients and after the onset of T1DM in 8). Height SDS patterns during GH therapy in the 11 patients affected by GH deficiency ranged from -0.3 to +3.1 SDS. In the 8 diabetic patients in whom GH was added subsequently, mean insulin dose increased during the first 6 months of therapy from 0.7 ± 0.2 to 1.0 ± 0.2 U/kg (p = 0.004). HbA1c was unchanged during the first 6 months of combined therapy. Conclusions: Most Italian physicians do not consider prescribing the combined GH-insulin therapy in diabetic children with growth problems. However, the results of the 17 patients identified would confirm that the combined therapy was feasible and only caused mild insulin resistance. GH therapy was effective in promoting growth in most patients and did not affect diabetes metabolic contro

Dysfunctional mitochondria accumulate in a skeletal muscle knockout model of Smn1, the causal gene of spinal muscular atrophy

The approved gene therapies for spinal muscular atrophy (SMA), caused by loss of survival motor neuron 1 (SMN1), greatly ameliorate SMA natural history but are not curative. These therapies primarily target motor neurons, but SMN1 loss has detrimental effects beyond motor neurons and especially in muscle. Here we show that SMN loss in mouse skeletal muscle leads to accumulation of dysfunctional mitochondria. Expression profiling of single myofibers from a muscle specific Smn1 knockout mouse model revealed down-regulation of mitochondrial and lysosomal genes. Albeit levels of proteins that mark mitochondria for mitophagy were increased, morphologically deranged mitochondria with impaired complex I and IV activity and respiration and that produced excess reactive oxygen species accumulated in Smn1 knockout muscles, because of the lysosomal dysfunction highlighted by the transcriptional profiling. Amniotic fluid stem cells transplantation that corrects the SMN knockout mouse myopathic phenotype restored mitochondrial morphology and expression of mitochondrial genes. Thus, targeting muscle mitochondrial dysfunction in SMA may complement the current gene therapy