Voluntarios y Ex Voluntarios: Perfiles de Participación Ciudadana a Través del Voluntariado

The study described is part of a broader longitudinal and multi-methodological research project aimed at investigating volunteerism in young people, in order to understand the reasons for the initial choice to volunteer but, more specifically, the reasons to sustain or quit voluntary involvement, as well as the effects of volunteerism. Eighteen volunteers and 18 ex-volunteers, 50% male and 50% female, aged between 22 and 29 years old, from 2 regions in northern Italy (Lombardy and Emilia Romagna) participated in in-depth interviews. The paper-and-pencil analysis of the interview pointed to the emergence of several core categories: motivations to volunteer, relations within the organization, influence of family, and effects of volunteerism, especially as related to the process of identity and citizenship construction. On the basis of these categories, 4 typologies were identified: 2 with respect to volunteers (producers of active citizenship and volunteers for personal necessity) and 2 related specifically to ex-volunteers (ex-volunteers witnesses for solidarity and active citizenship and ex-volunteers by chance). El estudio descrito es parte de un proyecto de investigación longitudinal y multi-metodológico más amplio sobre el voluntariado juvenil, realizado con el propósito de entender las razones que tuvieron los jóvenes para elegir el voluntariado y, específicamente, las razones para mantener o abandonar el compromiso, así como los efectos de dicho voluntariado. Participaron en entrevistas en profundidad 18 voluntarios y 18 ex-voluntarios, 50% hombres y 50% mujeres, entre 22 y 29 años de edad, de 2 regiones del norte de Italia (Lombardía y Emilia Romagna). El análisis de la entrevista de lápiz y papel permitió trazar varias categorías centrales: las motivaciones al voluntariado, las relaciones dentro de la organización, la influencia de la familia y los efectos del propio voluntariado, especialmente en relación con el proceso de construcción de identidad y ciudadanía. A partir de estas categorías fueron identificadas 4 tipologías: 2 respecto de los voluntarios (voluntarios productores de ciudadanía activa y voluntarios por necesidad personal) y 2 respecto de los ex voluntarios (ex voluntarios testigos de solidaridad y ciudadanía activa y ex voluntarios por azar)

I am going to make the most out of it! Italian university Credit Mobility Students' social representations of alcohol use during study abroad experiences

The aim was to explore shared representations of alcohol use in students who were to travel abroad to study. Focus group data from Italian students (N = 69) were collected. Analyses used Grounded Theory Methodology and were informed by the four key components of Social Representation Theory (cognition, emotion, attitude and behavioural intentions). The study abroad experience was described as one that would involve an increase in alcohol consumption compared to pre-departure levels. Reasons given included greater social and leisure opportunities involving alcohol, reduced social control and features of the host country environment. Opportunities to intervene and address risky alcohol use in this group are discussed

Young People and Volunteerism: A Model of Sustained Volunteerism During the Transition to Adulthood

Abstract A model of sustained volunteerism in young people is proposed. The longitudinal study addresses the questions ''Why do young people decide to continue to volunteer over an extended period of time?'' There were 158 volunteers (82 female and 76 male). The volunteers completed measures of motivation to volunteer, integration, and satisfaction with the organization, merged effects due to voluntary service, social support, identity, and intention to volunteer on a first (Time 1) and a second research wave (Time 2). Results show that both dispositional and organizational variables are important in determining long-term volunteerism in young people and confirmed that role identity is the best predictor of intention to volunteer

The Effect of the Psychological Sense of Community on the Psychological Well-Being in Older Volunteers

Ageing populations across Europe are increasing. Communities have an important role in not only engaging this segment of the population but also in helping them to make them feel "part of something" (local or global) in order to favour their psychological well-being. The purpose of this paper is to study the effects of volunteering and being connected in one's community on well-being. The present paper will test an older volunteers' psychological well-being model. 143 older volunteers completed measures of religiousness, sense of global responsibility, psychological sense of community, generativity, motivation to volunteer and a profile of mood states. Data show that a psychological sense of community has a key role in the study of older volunteerism due to its impact on well-being. Service agencies and administrations can develop campaigns to sustain older volunteerism in order to increase well-being and reduce social costs

Surface area of lipid membranes regulates the DNA-binding capacity of cationic liposomes.

We have applied electrophoresis on agarose gels to investigate the DNA-binding capacity of cationic liposomes made of cationic DC-cholesterol and neutral dioleoylphosphatidylethanolamine as a function of membrane charge density and cationic lipid/DNA charge ratio. While each cationic liposome formulation exhibits a distinctive DNA-protection ability, here we show that such a capacity is universally regulated by surface area of lipid membranes available for binding in an aspecific manner. The relevance of DNA protection for gene transfection is also discussed

On the correlation between phase evolution of lipoplexes/anionic lipid mixtures and DNA release

We investigated the structural evolution of three lipoplex formulations when interacting with anionic lipids by synchrotron small angle x-ray diffraction, while the extent of DNA release from lipoplexes by anionic lipids was evaluated by gel electrophoresis. Lipoplexes formed lamellar phases when mixed with anionic dioleoylphosphatidylglycerol (DOPG), while promoting the formation of nonbilayer structures when mixed with anionic dioleoylphosphatidic acid (DOPA). However, lipoplexes exhibited a virtually identical extent of DNA release when mixed with DOPG or DOPA. Thus, the recently proposed correlation between the formation of nonlamellar phases in lipoplex/anionic lipid mixtures and the increase of DNA release does not seem to exist

Transfection efficiency boost by designer multicomponent lipoplexes

Cationic liposome-DNA complexes (lipoplexes) have emerged as leading nonviral gene carriers in worldwide gene therapy clinical trials. Arriving at therapeutic dosages requires the full understanding of the mechanism of transfection. We investigated the correlation between structural evolution of multicomponent lipoplexes when interacting with cellular lipids, the extent of DNA release and the efficiency in transfecting mouse fibroblast (NIH 3T3), ovarian (CHO) and tumoral myofibroblast-like (A17) cell lines. We show, for the first time, that the transfection pattern increases monotonically with the number of lipid components and further demonstrate by means of synchrotron small angle X-ray scattering (SAXS) that structural changes of lipoplexes induced by cellular lipids correlate with the transfection efficiency. Specifically, inefficient lipoplexes either fused too rapidly upon interaction with anionic lipids or, alternatively, are found to be extremely resistant to solubilization. The most efficient lipoplex formulations exhibited an intermediate behaviour. The extent of DNA unbinding (measured by electrophoresis on agarose gel) correlates with structural evolution of the lipoplexes but DNA-release does not scale with the extent of transfection. The general meaning of our results is of broad interest in the field of non-viral gene delivery: rational adjusting of lipoplex composition to generate the proper interaction between lipoplexes and cellular lipids may be the most appropriate strategy in optimizing synthetic lipid transfection agents