Nonlinear actuator disk theory and flow field calculations, including nonuniform loading

Actuator disk theory and flow field calculations for propeller induced flow with nonuniform circulation distributio

Distribution of Soybean Cyst Nematode in Nebraska

A survey of 552 soybean fields in 20 counties in Nebraska in 1986-88 revealed 35 fields infested with the soybean cyst nematode (SCN), Heterodera glycines. Identification was confirmed with a greenhouse bioassay, using \u27Lee 74\u27 soybean, and by the application of a DNA hybridization probe derived from SCN mitochondrial DNA. Most of the SCN-infested fields were located on the Missouri River floodplain and in the southeastern corner of the state

Progress on reducing acrylamide levels in potato crisps in Europe, 2002 to 2019

European Snacks Association (ESA) data on acrylamide in potato crisps from 2002 to 2019 (99704 observations) were analysed. Acrylamide levels have plateaued since 2011, although the lowest mean so far was attained in 2018 at 353 ± 2.7 ng g-1: a 54% reduction since 2002. The 85th, 90th and 95th quantiles did show evidence of continued downward progress, the 90th quantile being lower than the 750 ng g-1 European Benchmark Level from 2017 to 2019. A smaller dataset from the European Food Safety Authority (2124 observations) for 2011-2018, was also analysed. The yearly means were higher than those of the ESA data, but showed a fall in average acrylamide from 715 ± 40.5 ng g-1 in 2015 to 505 ±28.5 ng g-1 in 2018, as well as steep falls in the 85th, 90th and 95th quantiles. Nevertheless, even the 85th quantile remained above the 750 ng g-1 Benchmark Level. The ESA data showed a reduction in the proportion of samples with acrylamide exceeding 750 ng g-1, from over 40 % in 2002 to 7.75 % in 2019. Seasonality was evident, with highest acrylamide levels from November to May. Crisp type had little effect except that thicker types had a higher proportion of samples containing >750 ng g-1 acrylamide. Analysis of the region of origin in Europe of the final product revealed improvements in the east and north. Geographical factors combined with seasonality continued to be problematic but was also an aspect in which progress was most evident. The findings show that improvements have been made in reducing the number of samples with very high levels of acrylamide, but do not suggest that mean acrylamide levels could be reduced substantially below where they have been since 2011, or that levels could be kept consistently below the current Benchmark Level

Estimating Protein-Ligand Binding Affinity using High- Throughput Screening by NMR

Many of today’s drug discovery programs utilize high-throughput screening methods that rely on quick evaluations of protein activity to rank potential chemical leads. By monitoring biologically relevant protein-ligand interactions, NMR can provide a means to validate these discovery leads and to optimize the drug discovery process. NMR-based screens typically use a change in chemical shift or linewidth to detect a protein-ligand interaction. However, the relatively low throughput of current NMR screens and their high demand on sample requirements generally makes it impractical to collect complete binding curves to measure the affinity for each compound in a large and diverse chemical library. As a result, NMR ligand screens are typically limited to identifying candidates that bind to a protein and do not give any estimate of the binding affinity. To address this issue, a methodology has been developed to rank binding affinities for ligands based on NMR-based screens that use 1D 1H NMR line-broadening experiments. This method was demonstrated by using it to estimate the dissociation equilibrium constants for twelve ligands with the protein human serum albumin (HSA). The results were found to give good agreement with previous affinities that have been reported for these same ligands with HSA

Estimating Protein-Ligand Binding Affinity using High- Throughput Screening by NMR

Many of today’s drug discovery programs utilize high-throughput screening methods that rely on quick evaluations of protein activity to rank potential chemical leads. By monitoring biologically relevant protein-ligand interactions, NMR can provide a means to validate these discovery leads and to optimize the drug discovery process. NMR-based screens typically use a change in chemical shift or linewidth to detect a protein-ligand interaction. However, the relatively low throughput of current NMR screens and their high demand on sample requirements generally makes it impractical to collect complete binding curves to measure the affinity for each compound in a large and diverse chemical library. As a result, NMR ligand screens are typically limited to identifying candidates that bind to a protein and do not give any estimate of the binding affinity. To address this issue, a methodology has been developed to rank binding affinities for ligands based on NMR-based screens that use 1D 1H NMR line-broadening experiments. This method was demonstrated by using it to estimate the dissociation equilibrium constants for twelve ligands with the protein human serum albumin (HSA). The results were found to give good agreement with previous affinities that have been reported for these same ligands with HSA

Global Opportunities to Increase Agricultural Independence Through Phosphorus Recycling

Food production hinges largely upon access to phosphorus (P) fertilizer. Most fertilizer P used in the global agricultural system comes from mining of nonrenewable phosphate rock deposits located within few countries. However, P contained in livestock manure or urban wastes represents a recyclable source of P. To inform development of P recycling technologies and policies, we examined subnational, national, and global spatial patterns for two intersections of land use affording high P recycling potential: (a) manure‐rich cultivated areas and (b) populous cultivated areas. In turn, we examined overlap between P recycling potential and nation‐level P fertilizer import dependency. Populous cultivated areas were less abundant globally than manure‐rich cultivated areas, reflecting greater segregation between crops and people compared to crops and livestock, especially in the Americas. Based on a global hexagonal grid (290‐km2 grid cell area), disproportionately large shares of subnational “hot spots” for P recycling potential occurred in India, China, Southeast Asia, Europe, and parts of Africa. Outside of China, most of the remaining manure‐rich or populous cultivated areas occurred within nations that had relatively high imports of P fertilizer (net P import:consumption ratios ≥0.4) or substantial increases in fertilizer demand between the 2000s (2002–2006) and 2010s (2010–2014). Manure‐rich cultivated grid cells (those above the 75th percentiles for both manure and cropland extent) represented 12% of the global grid after excluding cropless cells. Annually, the global sum of animal manure P was at least 5 times that contained in human excreta, and among cultivated cells the ratio was frequently higher (median = 8.9). The abundance of potential P recycling hot spots within nations that have depended on fertilizer imports or experienced rising fertilizer demand could prove useful for developing local P sources and maintaining agricultural independence

Molecular Differentiation of Alfalfa Weevil Strains (Coleoptera: Curculionidae)

Mitochondrial DNA was amplified and sequenced from eastern, western, and Egyptian strains of alfalfa weevil, Hypera postica (Gyllenhal). Eastern and Egyptian weevils differed at only 2 nucleotide sites in 1,031 base pairs sequenced; western weevils differed by 5% sequence divergence. Three restriction sites were identified which separated eastern and western haplotypes. No intrastrain polymorphism was detected in 150 weevils from Nebraska. Collections from Lincoln in eastern Nebraska and Scottsbluff in western Nebraska were fixed for the eastern and western haplotypes, respectively. Eastern and western haplotypes were found together in the same fields in a broad overlap region in central Nebraska

Using Representation Theorems for Proving Polynomials Non-negative

Proving polynomials non-negative when variables range on a subset of numbers (e.g., [0, +∞)) is often required in many applications (e.g., in the analysis of program termination). Several representations for univariate polynomials P that are non-negative on [0, +∞) have been investigated. They can often be used to characterize the property, thus providing a method for checking it by trying a match of P against the representation. We introduce a new characterization based on viewing polynomials P as vectors, and find the appropriate polynomial basis B in which the non-negativeness of the coordinates [P]B representing P in B witnesses that P is non-negative on [0, +∞). Matching a polynomial against a representation provides a way to transform universal sentences ∀x ∈ [0, +∞) P(x) ≥ 0 into a constraint solving problem which can be solved by using efficient methods. We consider different approaches to solve both kind of problems and provide a quantitative evaluation of performance that points to an early result by P´olya and Szeg¨o’s as an appropriate basis for implementations in most cases.Lucas Alba, S. (2014). Using Representation Theorems for Proving Polynomials Non-negative. En Artificial Intelligence and Symbolic Computation: 12th International Conference, AISC 2014, Seville, Spain, December 11-13, 2014. Proceedings. Springer Verlag (Germany). 21-33. doi:10.1007/978-3-319-13770-4_4S2133Alarcón, B., Gutiérrez, R., Lucas, S., Navarro-Marset, R.: Proving Termination Properties with mu-term. In: Johnson, M., Pavlovic, D. (eds.) AMAST 2010. LNCS, vol. 6486, pp. 201–208. Springer, Heidelberg (2011)Basu, S., Pollack, R., Roy, M.-F.: Algorithms in Real Algebraic Geometry. Springer, Berlin (2006)Bernstein, S.: Démonstration du théorème de Weierstrass fondée sur le calcul des probabilités. Communic. Soc. Math. de Kharkow 13(2), 1–2 (1912)Bernstein, S.: Sur la répresentation des polynômes positifs. Communic. Soc. Math. de Kharkow 14(2), 227–228 (1915)Borralleras, C., Lucas, S., Oliveras, A., Rodríguez, E., Rubio, A.: SAT Modulo Linear Arithmetic for Solving Polynomial Constraints. Journal of Automated Reasoning 48, 107–131 (2012)Boudaoud, F., Caruso, F., Roy, M.-F.: Certificates of Positivity in the Bernstein Basis. Discrete Computational Geometry 39, 639–655 (2008)Choi, M.D., Lam, T.Y., Reznick, B.: Sums of squares of real polynomials. In: Proc. of the Symposium on Pure Mathematics, vol. 4, pp. 103–126. American Mathematical Society (1995)Contejean, E., Marché, C., Tomás, A.-P., Urbain, X.: Mechanically proving termination using polynomial interpretations. Journal of Automated Reasoning 32(4), 315–355 (2006)Hilbert, D.: Über die Darstellung definiter Formen als Summe von Formenquadraten. Mathematische Annalen 32, 342–350 (1888)Hong, H., Jakuš, D.: Testing Positiveness of Polynomials. Journal of Automated Reasoning 21, 23–38 (1998)Karlin, S., Studden, W.J.: Tchebycheff systems: with applications in analysis and statistics. Interscience, New York (1966)Lucas, S.: Polynomials over the reals in proofs of termination: from theory to practice. RAIRO Theoretical Informatics and Applications 39(3), 547–586 (2005)Polya, G., Szegö, G.: Problems and Theorems in Analysis II. Springer (1976)Powers, V., Reznick, B.: Polynomials that are positive on an interval. Transactions of the AMS 352(10), 4677–4692 (2000)Powers, V., Wörmann, T.: An algorithm for sums of squares of real polynomials. Journal of Pure and Applied Algebra 127, 99–104 (1998

Effects of The Novel Endocannabinoid Uptake Inhibitor, LY2183240, on Fear-Potentiated Startle and Alcohol-Seeking Behaviors in Mice Selectively Bred for High Alcohol Preference

Rationale Alcohol-use disorders often occur together with anxiety disorders in humans which may be partly due to common inherited genetic factors. Evidence suggests that the endocannabinoid system (ECS) is a promising therapeutic target for the treatment of individuals with anxiety and/or alcohol-use disorders. Objectives The present study assessed the effects of a novel endocannabinoid uptake inhibitor, LY2183240, on anxiety- and alcohol-seeking behaviors in a unique animal model that may represent increased genetic risk to develop co-morbid anxiety and alcohol-use disorders in humans. Mice selectively bred for high alcohol preference (HAP) show greater fear-potentiated startle (FPS) than mice selectively bred for low alcohol preference (LAP). We examined the effects of LY2183240 on the expression of FPS in HAP and LAP mice and on alcohol-induced conditioned place preference (CPP) and limited-access alcohol drinking behavior in HAP mice. Results Repeated administration of LY2183240 (30 mg/kg) reduced the expression of FPS in HAP but not LAP mice when given prior to a second FPS test 48 hrs after fear conditioning. Both the 10 and 30 mg/kg doses of LY2183240 enhanced the expression of alcohol-induced CPP and this effect persisted in the absence of the drug. LY2183240 did not alter limited-access alcohol drinking behavior, unconditioned startle responding, or locomotor activity. Conclusions These findings suggest that ECS modulation influences both conditioned fear and conditioned alcohol reward behavior. LY2183240 may be an effective pharmacotherapy for individuals with anxiety disorders, such as post-traumatic stress disorder, but may not be appropriate for individuals with co-morbid anxiety and alcohol-use disorders