65 research outputs found
Inhibition of dipeptidyl peptidase IV and xanthine oxidase by amino acids and dipeptides
peer-reviewedXanthine oxidase (XO) and dipeptidyl peptidase IV (DPP-IV) inhibition by amino acids and dipeptides was studied. Trp and Trp-containing dipeptides (Arg-Trp, Trp-Val, Val-Trp, Lys-Trp and Ile-Trp) inhibited XO. Three amino acids (Met, Leu and Trp) and eight dipeptides (Phe-Leu, Trp-Val, His-Leu, Glu-Lys, Ala-Leu, Val-Ala, Ser-Leu and Gly-Leu) inhibited DPP-IV. Trp and Trp-Val were multifunctional inhibitors of XO and DPP-IV. Lineweaver and Burk analysis showed that Trp was a non-competitive inhibitor of XO and a competitive inhibitor of DPP-IV. Molecular docking with Autodock Vina was used to better understand the interaction of the peptides with the active site of the enzyme. Because of the non-competitive inhibition observed, docking of Trp-Val to the secondary binding sites of XO and DPP-IV is required. Trp-Val was predicted to be intestinally neutral (between 25% and 75% peptide remaining after 60 min simulated intestinal digestion). These results are of significance for the reduction of reactive oxygen species (ROS) and the increase of the half-life of incretins by food-derived peptides. (C) 2013 Elsevier Ltd. All rights reserved.ACCEPTEDpeer-reviewe
A Multi-wavelength View of the TeV Blazar Markarian 421: Correlated Variability, Flaring, and Spectral Evolution
We report results from a multi-wavelength monitoring campaign on Mrk 421 over
the period of 2003-2004. The source was observed simultaneously at TeV and
X-ray energies, with supporting observations frequently carried out at optical
and radio wavelengths. The large amount of simultaneous data has allowed us to
examine the variability of Mrk 421 in detail. The variabilities are generally
correlated between the X-ray and gamma-ray bands, although the correlation
appears to be fairly loose. The light curves show the presence of flares with
varying amplitudes on a wide range of timescales both at X-ray and TeV
energies. Of particular interest is the presence of TeV flares that have no
coincident counterparts at longer wavelengths, because the phenomenon seems
difficult to understand in the context of the proposed emission models for TeV
blazars. We have also found that the TeV flux reached its peak days before the
X-ray flux during a giant flare in 2004. Such a difference in the development
of the flare presents a further challenge to the emission models. Mrk 421
varied much less at optical and radio wavelengths. Surprisingly, the normalized
variability amplitude in optical seems to be comparable to that in radio,
perhaps suggesting the presence of different populations of emitting electrons
in the jet. The spectral energy distribution (SED) of Mrk 421 is seen to vary
with flux, with the two characteristic peaks moving toward higher energies at
higher fluxes. We have failed to fit the measured SEDs with a one-zone SSC
model; introducing additional zones greatly improves the fits. We have derived
constraints on the physical properties of the X-ray/gamma-ray flaring regions
from the observed variability (and SED) of the source. The implications of the
results are discussed. (Abridged)Comment: 32 pages, 12 figures, to appear in Ap
The TeV spectrum of H1426+428
The BL Lac object H1426+428 was recently detected as a high energy gamma-ray
source by the VERITAS collaboration (Horan et al. 2002). We have reanalyzed the
2001 portion of the data used in the detection in order to examine the spectrum
of H1426+428 above 250 GeV. We find that the time-averaged spectrum agrees with
a power law of the shape dF/dE = 10^(-7.31 +- 0.15(stat) +- 0.16(syst)) x
E^(-3.50 +- 0.35(stat) +- 0.05(syst)) m^(-2)s^(-1)TeV^(-1) The statistical
evidence from our data for emission above 2.5 TeV is 2.6 sigma. With 95% c.l.,
the integral flux of H1426+428 above 2.5 TeV is larger than 3% of the
corresponding flux from the Crab Nebula. The spectrum is consistent with the
(non-contemporaneous) measurement by Aharonian et al. (2002) both in shape and
in normalization. Below 800 GeV, the data clearly favours a spectrum steeper
than that of any other TeV Blazar observed so far indicating a difference in
the processes involved either at the source or in the intervening space.Comment: LaTeX, 8 pages, 4 figures, accepted for publication in Ap
Search for High Energy Gamma Rays from an X-ray Selected Blazar Sample
Our understanding of blazars has been greatly increased in recent years by
extensive multi-wavelength observations, particularly in the radio, X-ray and
gamma-ray regions. Over the past decade the Whipple 10m telescope has
contributed to this with the detection of 5 BL Lacertae objects at very high
gamma-ray energies. The combination of multi-wavelength data has shown that
blazars follow a well-defined sequence in terms of their broadband spectral
properties. Together with providing constraints on emission models, this
information has yielded a means by which potential sources of TeV emission may
be identified and predictions made as to their possible gamma-ray flux. We have
used the Whipple telescope to search for TeV gamma-ray emission from eight
objects selected from a list of such candidates. No evidence has been found for
VHE emission from the objects in our sample, and upper limits have been derived
for the mean gamma-ray flux above 390GeV. These flux upper limits are compared
with the model predictions and the implications of our results for future
observations are discussed.Comment: 15 pages, 2 figures, Accepted for publication in Ap
A Search for TeV Gamma-Ray Emission from High-Peaked Flat Spectrum Radio Quasars Using the Whipple Air-Cherenkov Telescope
Blazars have traditionally been separated into two broad categories based
upon their optical emission characteristics; BL Lacs, with faint or no emission
lines, and flat spectrum radio quasars (FSRQs) with prominent, broad emission
lines. The spectral energy distribution of FSRQs has generally been thought of
as being more akin to the low-peaked BL Lacs, which exhibit a peak in the
infrared region of the spectrum, as opposed to high-peaked BL Lacs (HBLs),
which exhibit a peak in UV/X-ray region of the spectrum. All blazars currently
confirmed as sources of TeV emission are HBLs. Recent surveys have found
several FSRQs exhibiting spectral properties similar to HBLs, particularly the
synchrotron peak frequency. These objects are potential sources of TeV emission
according to several models of blazar jet emission and blazar evolution.
Measurements of TeV flux or upper limits could impact existing theories
explaining the links between different blazar types and could have a
significant impact on our understanding of the nature of objects that are
capable of TeV emission. In particular, the presence (or absence) of TeV
emission from FSRQs could confirm (or cast doubt upon) recent evolutionary
models that expect intermediate objects in a transitionary state between FSRQ
and BL Lac. The Whipple 10 meter imaging air-Cherenkov gamma-ray telescope is
well suited for TeV gamma-ray observations. Using the Whipple telescope, we
have taken data on a small selection of nearby(z<0.1 in most cases),
high-peaked FSRQs. Although one of the objects, B2 0321+33, showed marginal
evidence of flaring, no significant emission was detected. The implications of
this paucity of emission and the derived upper limits are discussed.Comment: accepted for publication in Astrophysical Journa
A Very High Energy Gamma-Ray Spectrum of 1ES 2344+514
The BL Lacertae (BL Lac) object 1ES 2344+514 (1ES 2344), at a redshift of
0.044, was discovered as a source of very high energy (VHE) gamma rays by the
Whipple Collaboration in 1995 \citep{2344Catanese98}. This detection was
recently confirmed by the HEGRA Collaboration \citep{2344Hegra03}. As is
typical for high-frequency peaked blazars, the VHE gamma-ray emission is highly
variable. On the night of 20 December, 1995, a gamma-ray flare of 5.3-sigma
significance was detected, the brightest outburst from this object to-date. The
emission region is compatible with a point source. The spectrum between 0.8 TeV
and 12.6 TeV can be described by a power law . Comparing the spectral
index with that of the other five confirmed TeV blazars, the spectrum of 1ES
2344 is similar to 1ES 1959+650, located at almost the same distance. The
spectrum of 1ES 2344 is steeper than the brightest flare spectra of Markarian
421 (Mrk~421) and Markarian 501 (Mrk~501), both located at a distance about 2/3
that of 1ES 2344, and harder than the spectra of PKS 2155-304 and H~1426+428,
which are located almost three times as far. This trend is consistent with
attenuation caused by the infrared extragalactic background radiation.Comment: 27 pages, 11 figures, accepted by Ap
Reconstructing grassland fire history using sedimentary charcoal: Considering count, size and shape
Citation: Leys, B. A., Commerford, J. L., & McLauchlan, K. K. (2017). Reconstructing grassland fire history using sedimentary charcoal: Considering count, size and shape. Plos One, 12(4), 15. doi:10.1371/journal.pone.0176445Fire is a key Earth system process, with 80% of annual fire activity taking place in grassland areas. However, past fire regimes in grassland systems have been difficult to quantify due to challenges in interpreting the charcoal signal in depositional environments. To improve reconstructions of grassland fire regimes, it is essential to assess two key traits: (1) charcoal count, and (2) charcoal shape. In this study, we quantified the number of charcoal pieces in 51 sediment samples of ponds in the Great Plains and tested its relevance as a proxy for the fire regime by examining 13 potential factors influencing charcoal count, including various fire regime components (e.g. the fire frequency, the area burned, and the fire season), vegetation cover and pollen assemblages, and climate variables. We also quantified the width to length (W: L) ratio of charcoal particles, to assess its utility as a proxy of fuel types in grassland environments by direct comparison with vegetation cover and pollen assemblages. Our first conclusion is that charcoal particles produced by grassland fires are smaller than those produced by forest fires. Thus, a mesh size of 120 mu m as used in forested environments is too large for grassland ecosystems. We recommend counting all charcoal particles over 60 mu m in grasslands and mixed grass-forest environments to increase the number of samples with useful data. Second, a W: L ratio of 0.5 or smaller appears to be an indicator for fuel types, when vegetation surrounding the site is before composed of at least 40% grassland vegetation. Third, the area burned within 1060m of the depositional environments explained both the count and the area of charcoal particles. Therefore, changes in charcoal count or charcoal area through time indicate a change in area burned. The fire regimes of grassland systems, including both human and climatic influences on fire behavior, can be characterized by long-term charcoal records
A Graph Algorithmic Approach to Separate Direct from Indirect Neural Interactions
Network graphs have become a popular tool to represent complex systems
composed of many interacting subunits; especially in neuroscience, network
graphs are increasingly used to represent and analyze functional interactions
between neural sources. Interactions are often reconstructed using pairwise
bivariate analyses, overlooking their multivariate nature: it is neglected that
investigating the effect of one source on a target necessitates to take all
other sources as potential nuisance variables into account; also combinations
of sources may act jointly on a given target. Bivariate analyses produce
networks that may contain spurious interactions, which reduce the
interpretability of the network and its graph metrics. A truly multivariate
reconstruction, however, is computationally intractable due to combinatorial
explosion in the number of potential interactions. Thus, we have to resort to
approximative methods to handle the intractability of multivariate interaction
reconstruction, and thereby enable the use of networks in neuroscience. Here,
we suggest such an approximative approach in the form of an algorithm that
extends fast bivariate interaction reconstruction by identifying potentially
spurious interactions post-hoc: the algorithm flags potentially spurious edges,
which may then be pruned from the network. This produces a statistically
conservative network approximation that is guaranteed to contain non-spurious
interactions only. We describe the algorithm and present a reference
implementation to test its performance. We discuss the algorithm in relation to
other approximative multivariate methods and highlight suitable application
scenarios. Our approach is a tractable and data-efficient way of reconstructing
approximative networks of multivariate interactions. It is preferable if
available data are limited or if fully multivariate approaches are
computationally infeasible.Comment: 24 pages, 8 figures, published in PLOS On
Spectrum of very high energy gamma-rays from the blazar 1ES 1959+650 during flaring activity in 2002
The blazar 1ES 1959+650 was observed in a flaring state with the Whipple 10 m Imaging Atmospheric Cerenkov Telescope in 2002 May. A spectral analysis has been carried out on the data from that time period, and the resulting very high energy gamma-ray spectrum (E greater than or equal to 316 GeV) can be well fitted by a power law of differential spectral index alpha = 2.78 +/- 0.12(stat) +/- 0.21(sys). On 2002 June 4, the source flared dramatically in the gamma-ray range without any coincident increase in the X-ray emission, providing the first unambiguous example of an orphan\u27\u27 gamma-ray flare from a blazar. The gamma-ray spectrum for these data can also be described by a simple power-law fit with alpha = 2.82 +/- 0.15(stat) +/- 0.30(sys). There is no compelling evidence for spectral variability or for any cutoff to the spectrum
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