Effect of Two Bacillus thuringiensis1 Proteins on Development of the Fall Armyworm2 after Seven-Day Exposure Efecto en el Desarrollo del Gusano Cogollero2 Después de Ser Expuesto por Siete Días a Dos Proteínas de Bacillus thuringiensis1

Field-evolved resistance of fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), to the bacterium Bacillus thuringiensis (Bt) has been reported worldwide as one of the most serious threats to the sustainability of Bt maize crop. Therefore, it is important to assess the magnitude of adult survival and the possibility of cross-resistance of fall armyworm neonates exposed to Bt proteins. In this study, bioassays were used to examine susceptibility of two field-collected Cry1Fa-resistant strains of fall armyworm from Puerto Rico (456RR, 512RR) and their crosses with a susceptible strain (Monsanto SS) (456SR-RS, 512SR-RS). LC50 values varied in both Cry1Fa-resistant strains and in their backcrosses with the susceptible strain. The two RR strains were more tolerant to Cry1Fa and Cry1Ac proteins in earlier instars of development than were their crosses. Greater survival to the adult stage was obtained in the 512 RR strain and their RS-SR crosses when exposed to all concentrations of Cry1Ac and 1Fa. Survival to adult in the 456 RR was much greater when exposed to Cry1Fa than to Cry1Ac. Adults of 456 RR and their crosses survived only when exposed to the lowest concentrations of Cry1Ac. Our data confirmed great resistance to Cry1Fa and Cry1Ac in S. frugiperda larvae from Puerto Rico. However, based on the larvae that survived the 7-day diet bioassay and developed to pupae and adult maturity on regular diet, their LC50 values were less for both resistant strains and their crosses

Preyssler Heteropolyacids in the Self-Etherification of 5-Hydroxymethylfurfural to 5,5′-[Oxybis(methylene)]bis-2-furfural Under Mild Reaction Conditions

The synthesis of 5,5′-[oxybis(methylene)]bis-2-furfural (OBMF) from 5-hydroxymethylfurfural (5-HMF) was studied using bulk and alumina-supported Preyssler heteropolyacids (HPAs). The formation of OBMF was related to the amount of Brønsted acid sites, and the lowest yield of OBMF was obtained with supported HPAs. However, the Lewis acidity of the HPA supported on Al2O3 favored the formation of 2,5-dimethylfurane. The effects of solvent, catalyst loading, temperature, and reaction time on the selectivity to OBMF from 5-HMF were studied to optimize OBMF production using bulk Preyssler HPAs; a yield of 84 % to OBMF was obtained at 5 h and 343 K. These results demonstrate that bulk Preyssler HPA is a good candidate for OBMF synthesis under mild reaction conditions.Fil: Páez, Alexander. Universidad Pedagógica y Tecnológica de Colombia; ColombiaFil: Rojas, Hugo A.. Universidad Pedagógica y Tecnológica de Colombia;Fil: Portilla Zúñiga, Omar Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas ; ArgentinaFil: Sathicq, Angel Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas ; ArgentinaFil: Afonso, Carlos A. M.. Universidade de Lisboa; PortugalFil: Romanelli, Gustavo Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas ; ArgentinaFil: Martínez, José J.. Universidad Pedagógica y Tecnológica de Colombia; Colombi

Construção e validação de um questionário para identificação de fatores associados ao desempenho escolar (FADE)

Se construyó una prueba psicológica para medir los Factores Asociados al Desempeño Escolar (FADE), siguiendo lineamientos de la Teoría Clásica de los Test y aspectos de medición y evaluación desde una estrategia multi-informante aplicada a los contextos escolares. La finalidad de la prueba es la identificación de debilidades y fortalezas asociadas al desempeño escolar en niños de primer grado de primaria del municipio de Pasto. La muestra utilizada fue de 277 participantes pertenecientes a instituciones públicas de carácter urbano y rural. La confiabilidad calculada a través del coeficiente KR20 fue de .92 para la prueba total. Se obtuvieron evidencias favorables para validez de contenido, criterio y constructo. Se establecieron normas de estandarización para la aplicación y calificación de la prueba. La evidencia encontrada da cuenta de que la prueba resulta útil para su aplicación práctica como herramienta en la evaluación clínica y su uso en investigación.Construiu-se um teste psicológico para medir os Fatores Associados ao Desempenho Escolar (FADE), seguindo as diretrizes da Teoría Clássica dos Testes e aspectos de medição e avaliação desde uma estratégia multi-informante aplicada aos contextos escolares. A finalidade do teste é a identificação de pontos fortes e pontos fracos associados ao desempenho escolar em crianças do primeiro ano do primário do município de Pasto. A mostra utilizada foi de 277 participantes pertencentes a instituições públicas de caráter urbano e rural. A confiabilidade calculada através do coeficiente KR20 foi de ,92 para a prova total. Obtiveram-se evidências favoráveis para validade de conteúdo, critério e constructo. Estabeleceram-se normas de padronização para a aplicação e qualificação da prova. A evidência encontrada mostra que a prova é útil para sua aplicação prática como ferramenta na avaliação clínica e seu uso em pesquisa.A psychological test was constructed to measure School Performance Associated Factors (FADE, by its Spanish acronym), following the guidelines of the Classical Theory of Tests and aspects of measuring and assessment from a multiple informant strategy applied to school settings. The purpose of the test is to identify weaknesses and strengths associated with school performance in first grade primary school children from the municipality of Pasto. The sample consisted of 277 participants from public institutions of urban and rural character. The reliability calculated by KR20 coefficient was 0.92 for the total test. Favorable evidence was obtained for content, criteria and construct validity. Standardized rules were established for the test administration and scoring. The evidence found shows that the test is useful for its practical application as a tool for clinical assessment and research

Procedimiento para implementar QoS en la capa de acceso en redes de próxima generación enfocado en el servicio de voz

Currently all services converge into a Next Generation Network and considering that today the requirements about quality of service are more stringent because of the same user requirements, it is necessary to propose a procedure of QoS to ensure an efficient operation of transporting the most critical services and real time; as the voice, ensuring the reduction of latency, jitter, packet loss and Eco. The CRC (Regulatory Commission of Communications) in Colombia applies the provisions of QoS for they can be used by telecommunications operators and they must be conform to the recommendations Y.1540 and Y.1541 ITU-T (International Telecommunication Union). The aim of this paper is to present a procedure for applying QoS mechanisms in NGN network in xDSL access in order to maintain quality of service in Voice over IP (VoIP), which allows economic and technically efficient provision of VoIP for customer and the telecommunications operator.En la actualidad, todos los servicios convergen en una Red de Próxima Generación [NGN]. Asimismo, las exigencias de calidad de servicio [QoS], por los requerimientos de los usuarios, son más estrictas, lo que hace necesario plantear procedimientos de QoS que garanticen una operación eficaz en el transporte de los servicios más críticos y de tiempo real –como la voz–, garantizando la disminución de los problemas de latencia, jitter, pérdida de paquetes y eco. Los operadores de Telecomunicaciones deben aplicar las regulaciones emitidas por la Comisión de Regulación de Comunicaciones de Colombia [CRC] y ajustarse a las recomendaciones Y.1540 y Y.1541 de la Unión Internacional de Telecomunicaciones [UIT]. Este documento presenta un procedimiento para aplicar mecanismos de QoS en una NGN en el acceso xDSL con el fin de mantener un nivel de QoS en Voz sobre IP (VoIP) que permita su provisión, con eficiencia económica y técnica, en favor tanto del cliente, como del operador de telecomunicaciones

Obtaining protoanemonin through selective oxidation of Dfructose and 5-(hydroxymethyl) furfural in a self-catalysed reaction

Although different ways of converting 5- (hydroxymethyl)furfural (1) to various substrates with high value have been sought, few transformations have obtained building blocks that can be very useful in the area of fine chemistry. Herein, we report the synthesis of protoanemonin (5-methylenefuran-2(5H)- one) from D-fructose via compound (1), a versatile γalkylidenebutenolide, using an efficient self-catalysed process with formic acid, with high reaction performance and selectivity (up to 94% yield and 98% conversion from (1), while 28% yield from Dfructose). This efficient and simple operational process involved a two-phase aqueous-organic system between chlorinated solvents (CHxCly) and hydrogen peroxide as the initial oxidizing agent. The reaction presents a key cleavage in the 5-hydroxymethyl moiety of (1), due to the Baeyer-Villiger oxidation (BVO) process that generates formic acid in situ. Ultimately, DFF and HMF were successfully obtained in 80% and 98% yield, respectively, starting from D-fructose and using Preyssler heteropolyacids as Brønsted acid catalysts under an atmosphere of oxygen in the absence of hydrogen peroxide.Fil: Martínez, José J.. Universidad Pedagógica y Tecnológica de Colombia; ColombiaFil: Páez, Luis A.. Universidad Pedagógica y Tecnológica de Colombia; ColombiaFil: Gutiérrez, Luisa F.. Universidad Pedagógica y Tecnológica de Colombia; ColombiaFil: Pardo Cuervo, Oscar H.. Universidad Pedagógica y Tecnológica de Colombia; ColombiaFil: Rojas, Hugo A.. Universidad Pedagógica y Tecnológica de Colombia; ColombiaFil: Romanelli, Gustavo Pablo. Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de la Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas "Dr. Jorge J. Ronco". Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Ciencias Aplicadas; ArgentinaFil: Portilla, Jaime. Universidad de Los Andes, Colombia; ColombiaFil: Castillo, Juan Carlos. Universidad Pedagógica y Tecnológica de Colombia; Colombia. Universidad de los Andes; ColombiaFil: Gamboa Becerra, Diana Paola. Universidad Pedagógica y Tecnológica de Colombia; Colombi

Soybean Flour and Wheat Germ Proportions in Artificial Diet and Their Effect on the Growth Rates of the Tobacco Budworm, Heliothis virescens

Soybean flour and wheat germ are the two most important protein components of wheat germ-based insect artificial diets. The effect of modifying the proportion of these two ingredients in a Noctuidae-specific diet was investigated utilizing the tobacco budworm Heliothis virescens (F.) (Lepidoptera: Noctuidae), with the goal of developing a suboptimal diet that, without drastically affecting this insect's growth and reproductive rates, could manifest subtle negative effects in this insect. The original diet formula contained 2.51% protein. When the proportions of soybean flour and wheat germ were changed to 2.15% protein the net reproductive rate of the first generation was significantly lower. In the second generation, the net reproductive rate, development time, percent female survivorship, fertility, intrinsic rate of increase, finite rate of increase and female longevity were significantly lower in both the 2.15% and 2.26% protein diets. The survival rate of immatures to the adult stage was 1% in the 2.05% protein diet in the first generation. Interestingly, females exposed to these suboptimal diets produced a significantly higher number of eggs but the survival of their larvae was significantly reduced. It is evident from these results that modifications to the protein content and the nutrient composition profile of the original wheat germ-based insect artificial formula can be used to produce subtle negative effects on the growth of tobacco budworm

Prevalence and risk factors associated with peripheral arterial disease in an adult population from Colombia

_Background:_ Cardiovascular diseases (CVD) are the most important cause of mortality in Latin America, while peripheral arterial disease (PAD) is the third leading cause of atherosclerotic cardiovascular morbidity. _Objective:_ To establish the prevalence of PAD and the distribution of traditional CVD risk factors in a population from the Department of Cauca, Colombia. Methods: A cross-sectional study was conducted on a total of 10,000 subjects aged ≥40 years, from 36 municipalities. An ankle -brachial index (ABI) ≤ 0.9 in either leg was used as diagnostic criterion of PAD. _Results:_ Overall PAD prevalence was 4.4% (4.7% females vs. 4.0% males), with diabetes being the most prevalent risk factor (23%). Among individuals self-reporting a history of acute myocardial infarction or stroke, PAD prevalence was 31.0% and 8.1%, respectively. After adjusting for potential confounders, PAD was signif

Vulnerabilidad y riesgo por plaguicidas en horticultura del cinturón verde en Córdoba, Argentina

El Cinturón Verde de la Ciudad de Córdoba (cvcc) en Argentina está marcado por la reproducción de relaciones desiguales, la informalidad y débil institucionalidad para la regulación de la exposición laboral a plaguicidas. Objetivo: visibilizar situaciones de vulnerabilidad y riesgo como construcción social material, que afecta a quienes trabajan en unidades productivas hortícolas del cvcc, asociadas a condiciones de vida y trabajo. Metodología: estudio epidemiológico descriptivo correlacional. Se conformó una muestra de 101 trabajadores / productores, con un nivel de confianza del 95%. Se obtuvo información mediante encuesta, indagando aspectos sociodemográficos, condiciones de vida, prácticas laborales, cultivos, estilos de vida y condiciones de salud del productor / trabajador y su familia. Se calcularon estadísticas descriptivas, se usó xi2 como test de hipótesis y análisis factorial de correspondencias múltiples. Resultados: encontramos diferencias en la situación de vulnerabilidad y riesgo en grupos de propietarios, arrendatario-medieros. En el primero se objetiva el riesgo vinculado a la falta de cuidados al usar plaguicidas; en el segundo se configuran situaciones de vulnerabilidad asociadas a la reproducción de relaciones desiguales. La comprensión de la vulnerabilidad y el riesgo desde la condición laboral, permite precisar aspectos de la exposición a plaguicidas a ser contemplados en programas de salud.Fil: Franchini, Carlos Germán. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud; Argentin

Short-lasting unilateral neuralgiform headache attacks with cranial autonomic symptoms (SUNA) secondary to epidermoid cyst in the right cerebellopontine angle successfully treated with surgery

Short-lasting unilateral neuralgiform headache attacks with conjunctival injection and tearing (SUNCT) syndrome is a rare headache syndrome classified among the trigeminal autonomic cephalalgias. It is usually idiopathic, although infrequent secondary forms have been described. Recently, the term short-lasting unilateral headache with cranial autonomic symptoms (SUNA) has been defined by the International Headache Society (ICHD-2) as similar to SUNCT with less prominent absent conjunctival injection and lacrimation. We report a patient with paroxysmal orbito-temporal pains, phenotypically suggesting SUNA, secondary to epidermoid cyst in the cerebellopontine angle which disappeared after tumor resection. Neuroimaging should be considered in all patients with SUNA, notably in those with atypical presentation as our patient who presented on examination trigeminal hypoesthesia and tinnitus. Realization of a brain MRI would rule out injuries that causes this type of syndrome

Preyssler Heteropolyacids in the Self-Etherification of 5-Hydroxymethylfurfural to 5,5′-[Oxybis(methylene)]bis-2-furfural Under Mild Reaction Conditions

The synthesis of 5,5′-[oxybis(methylene)]bis-2-furfural (OBMF) from 5-hydroxymethylfurfural (5-HMF) was studied using bulk and alumina-supported Preyssler heteropolyacids (HPAs). The formation of OBMF was related to the amount of Brønsted acid sites, and the lowest yield of OBMF was obtained with supported HPAs. However, the Lewis acidity of the HPA supported on Al2O3 favored the formation of 2,5-dimethylfurane. The effects of solvent, catalyst loading, temperature, and reaction time on the selectivity to OBMF from 5-HMF were studied to optimize OBMF production using bulk Preyssler HPAs; a yield of 84 % to OBMF was obtained at 5 h and 343 K. These results demonstrate that bulk Preyssler HPA is a good candidate for OBMF synthesis under mild reaction conditions.Centro de Investigación y Desarrollo en Ciencias Aplicada