The ABC of Governance Principles for Carbon Dioxide Removal Policy

Climate change mitigation actions, including those aimed at developing and scaling carbon dioxide removal (CDR) activities spanning the industrial, energy, and agroforestry sector, emerge in a context of internationally shared norms that include governance objectives, legal provisions and informal expectations, and societal expectations. Established governance principles provide normative orientation for policy including when targeting the development and scaling of CDR. Knowledge of these principles can guide effective discussion and evaluation of policy options. To facilitate discussion of mitigation options among experts and CDR practitioners, this study excerpts governance principles from legislative texts, the climate governance literature, and the CDR literature with relevance to CDR policy considerations. To illustrate the relevance of the governance principles found for evaluating policy options, we apply them to three technology groups of CDR: Bioenergy with Carbon Capture and Storage (BECCS), Direct Air Carbon Capture and Storage (DACCS), and forestry. This exercise indicates the importance of more intensive attention to the normative dimension of mitigation policies in ongoing deliberative and planning processes. Such efforts can help disentangle normative and factual dimensions and sources of (dis)agreement on the role of CDR in specific climate policy contexts

The ABC of Governance Principles for Carbon Dioxide Removal Policy

Climate change mitigation actions, including those aimed at developing and scaling carbon dioxide removal (CDR) activities spanning the industrial, energy, and agroforestry sector, emerge in a context of internationally shared norms that include governance objectives, legal provisions and informal expectations, and societal expectations. Established governance principles provide normative orientation for policy including when targeting the development and scaling of CDR. Knowledge of these principles can guide effective discussion and evaluation of policy options. To facilitate discussion of mitigation options among experts and CDR practitioners, this study excerpts governance principles from legislative texts, the climate governance literature, and the CDR literature with relevance to CDR policy considerations. To illustrate the relevance of the governance principles found for evaluating policy options, we apply them to three technology groups of CDR: Bioenergy with Carbon Capture and Storage (BECCS), Direct Air Carbon Capture and Storage (DACCS), and forestry. This exercise indicates the importance of more intensive attention to the normative dimension of mitigation policies in ongoing deliberative and planning processes. Such efforts can help disentangle normative and factual dimensions and sources of (dis)agreement on the role of CDR in specific climate policy contexts

Sporulenes, Heptaprenyl Metabolites from Bacillus subtilis Spores

Sporulene, a C35-terpenoid hydrocarbon with an unusual pentacyclic structure, is produced by Bacillus subtilis during sporulation.Harvard University. Microbial Sciences Initiative (Fellowship

Identification of a methylase required for 2-methylhopanoid production and implications for the interpretation of sedimentary hopanes

The rise of atmospheric oxygen has driven environmental change and biological evolution throughout much of Earth’s history and was enabled by the evolution of oxygenic photosynthesis in the cyanobacteria. Dating this metabolic innovation using inorganic proxies from sedimentary rocks has been difficult and one important approach has been to study the distributions of fossil lipids, such as steranes and 2-methylhopanes, as biomarkers for this process. 2-methylhopanes arise from degradation of 2-methylbacteriohopanepolyols (2-MeBHPs), lipids thought to be synthesized primarily by cyanobacteria. The discovery that 2-MeBHPs are produced by an anoxygenic phototroph, however, challenged both their taxonomic link with cyanobacteria and their functional link with oxygenic photosynthesis. Here, we identify a radical SAM methylase encoded by the hpnP gene that is required for methylation at the C-2 position in hopanoids. This gene is found in several, but not all, cyanobacteria and also in α -proteobacteria and acidobacteria. Thus, one cannot extrapolate from the presence of 2-methylhopanes alone, in modern environments or ancient sedimentary rocks, to a particular taxonomic group or metabolism. To understand the origin of this gene, we reconstructed the evolutionary history of HpnP. HpnP proteins from cyanobacteria, Methylobacterium species, and other α-proteobacteria form distinct phylogenetic clusters, but the branching order of these clades could not be confidently resolved. Hence,it is unclear whether HpnP, and 2-methylhopanoids, originated first in the cyanobacteria. In summary, existing evidence does not support the use of 2-methylhopanes as biomarkers for oxygenic photosynthesis

Sepsis related mortality of extremely low gestational age newborns after the introduction of colonization screening for multi-drug resistant organisms

Background: In 2013 German infection surveillance guidelines recommended weekly colonization screening for multidrug-resistant (MDRO) or highly epidemic organisms for neonatal intensive care units (NICUs) and extended hygiene measures based on screening results. It remains a matter of debate whether screening is worth the effort. We therefore aimed to evaluate sepsis related outcomes before and after the guideline update. Methods: The German Neonatal Network (GNN) is a prospective cohort study including data from extremely preterm infants between 22 + 0 and 28 + 6 gestational weeks born in 62 German level III NICUs. Results: Infants treated after guideline update (n = 8.903) had a lower mortality (12.5% vs. 13.8%, p = 0.036), reduced rates for clinical sepsis (31.4 vs. 42.8%, p < 0.001) and culture-proven sepsis (14.4% vs. 16.5%, p = 0.003) as compared to infants treated before update (n = 3.920). In a multivariate logistic regression analysis, nine pathogens of cultureproven sepsis were associated with sepsis-related death, e.g. Pseudomonas aeruginosa [OR 59 (19–180), p < 0.001)]. However, the guideline update had no significant effect on pathogen-specific case fatality, total sepsis-related mortality and culture-proven sepsis rates with MDRO. While the exposure of GNN infants to cefotaxime declined over time (31.1 vs. 40.1%, p < 0.001), the treatment rate with meropenem was increased (31.6 vs. 26.3%, p < 0.001). Conclusions: The introduction of weekly screening and extended hygiene measures is associated with reduced sepsis rates, but has no effects on sepsis-related mortality and sepsis with screening-relevant pathogens. The high exposure rate to meropenem should be a target of antibiotic stewardship programs

The ABC of Governance Principles for Carbon Dioxide Removal Policy

Climate change mitigation actions, including those aimed at developing and scaling carbon dioxide removal (CDR) activities spanning the industrial, energy, and agroforestry sector, emerge in a context of internationally shared norms that include governance objectives, legal provisions and informal expectations, and societal expectations. Established governance principles provide normative orientation for policy including when targeting the development and scaling of CDR. Knowledge of these principles can guide effective discussion and evaluation of policy options. To facilitate discussion of mitigation options among experts and CDR practitioners, this study excerpts governance principles from legislative texts, the climate governance literature, and the CDR literature with relevance to CDR policy considerations. To illustrate the relevance of the governance principles found for evaluating policy options, we apply them to three technology groups of CDR: Bioenergy with Carbon Capture and Storage (BECCS), Direct Air Carbon Capture and Storage (DACCS), and forestry. This exercise indicates the importance of more intensive attention to the normative dimension of mitigation policies in ongoing deliberative and planning processes. Such efforts can help disentangle normative and factual dimensions and sources of (dis)agreement on the role of CDR in specific climate policy contexts

The RND-family transporter, HpnN, is required for hopanoid localization to the outer membrane of Rhodopseudomonas palustris TIE-1

Rhodopseudomonas palustris TIE-1 is a Gram-negative bacterium that produces structurally diverse hopanoid lipids that are similar to eukaryotic steroids. Its genome encodes several homologues to proteins involved in eukaryotic steroid trafficking. In this study, we explored the possibility that two of these proteins are involved in intracellular hopanoid transport. R. palustris has a sophisticated membrane system comprising outer, cytoplasmic, and inner cytoplasmic membranes. It also divides asymmetrically, producing a mother and swarmer cell. We deleted genes encoding two putative hopanoid transporters that belong to the resistance–nodulation– cell division superfamily. Phenotypic analyses revealed that one of these putative transporters (HpnN) is essential for the movement of hopanoids from the cytoplasmic to the outer membrane, whereas the other (Rpal_4267) plays a minor role. C30 hopanoids, such as diploptene, are evenly distributed between mother and swarmer cells, whereas hpnN is required for the C35 hopanoid, bacteriohopanetetrol, to remain localized to the mother cell type. Mutant cells lacking HpnN grow like the WT at 30 °C but slower at 38 °C. Following cell division at 38 °C, the ΔhpnN cells remain connected by their cell wall, forming long filaments. This phenotype may be attributed to hopanoid mislocalization because a double mutant deficient in both hopanoid biosynthesis and transport does not form filaments. However, the lack of hopanoids severely compromises cell growth at higher temperatures more generally. Because hopanoid mutants only manifest a strong phenotype under certain conditions, R. palustris is an attractive model organism in which to study their transport and function

Histone Deacetylase 5 Is Overexpressed in Scleroderma Endothelial Cells and Impairs Angiogenesis via Repression of Proangiogenic Factors

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/134835/1/art39828_am.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/134835/2/art39828.pd

Characterization of the Channel Constriction Allowing the Access of the Substrate to the Active Site of Yeast Oxidosqualene Cyclase

In oxidosqualene cyclases (OSCs), an enzyme which has been extensively studied as a target for hypocholesterolemic or antifungal drugs, a lipophilic channel connects the surface of the protein with the active site cavity. Active site and channel are separated by a narrow constriction operating as a mobile gate for the substrate passage. In Saccharomyces cerevisiae OSC, two aminoacidic residues of the channel/constriction apparatus, Ala525 and Glu526, were previously showed as critical for maintaining the enzyme functionality. In this work sixteen novel mutants, each bearing a substitution at or around the channel constrictions, were tested for their enzymatic activity. Modelling studies showed that the most functionality-lowering substitutions deeply alter the H-bond network involving the channel/constriction apparatus. A rotation of Tyr239 is proposed as part of the mechanism permitting the access of the substrate to the active site. The inhibition of OSC by squalene was used as a tool for understanding whether the residues under study are involved in a pre-catalytic selection and docking of the substrate oxidosqualene