Neural Preparation For Step Initiation In Unpredictable Conditions With Age And Parkinson\u27s Disease

Mobility is essential for the independent lifestyle. However, as the US population ages, challenges to mobility start to arise, among them just the aging itself which leads to decreased postural stability, falls and the second most common neurodegenerative disease, that is Parkinson’s disease (PD). We decided to investigate step initiation as it is crucial to mobility: walking is not possible without the first step. Step initiation is impaired in PD. However, the impact of PD on the neural mechanisms of step initiation when some of the step parameters are unpredictable remains unexplored. Cortical preparation for step initiation can be assessed by beta event-related desynchronization (ERD) derived from electroencephalography (EEG) recordings. We hypothesized that subjects with PD would exhibit less cortical modulation between conditions of forward step initiation with and without prior knowledge of limb choice. Further, we hypothesized that decreased cortical modulation in PD would associate with a higher impairment of motor performance. Results identified that the group with PD exhibited decreased beta ERD amplitudes that were similar regardless of condition, whereas control subjects modulated beta ERD amplitudes between conditions, particularly in early stages of pre-movement processing in areas overlying sensory cortex. Subjects with PD presented with delayed and reduced postural preparation with increased step target error across both conditions and exhibited a greater incidence of multiple anticipatory postural adjustments (APAs) in the predictable relative to the unpredictable condition. Delayed postural preparation significantly correlated with lower amplitudes of beta ERD. We concluded that diminished early pre-movement processing over sensory cortex was concomitant with poor pre-selection of the stepping limb in predictable conditions and that a generally diminished amplitude of cortical pre-movement processing relates to delayed step initiation in people with PD. Furthermore, impaired mobility accompanies healthy aging, but there is a need for deeper understanding of how aging changes central control of motor behavior. Using previous study’s method, we compared cortical preparation for step initiation using beta ERD in young and older healthy subjects performing forward steps with and without prior knowledge of limb choice. Our results show that older subjects exhibited increased beta ERD amplitudes before the step regardless of whether they were informed of limb choice or not. Moreover, older subjects exhibited early increases in beta ERD in the “sensory” cluster of electrodes, but only when full limb-choice information was available. Behaviorally, the older subjects also exhibited shortened and increased anticipatory postural adjustments which led to earlier step initiation and similar swing-foot velocities but was also accompanied by greater target step placement errors and decreased postural stability. For the older group, condition-related increases in beta ERD amplitudes and stability correlated with condition-related prolongation of APA durations. We conclude that older subjects exhibited a spectrum across two strategies: (1) a “fast” strategy associated with decreased neural preparation that trades shortened step preparation and higher swing-foot velocity for target step errors and lowered postural stability; and (2) an “accurate” strategy associated with greater neural preparation, longer step-preparation time, and higher stability during step execution. In conclusion, this thesis provides more support for beta ERD as a useful tool for studying cortical preparation non-invasively. We have also established the importance of the signals recorded by “sensory” clusters: in subjects with PD the absence of beta ERD similar to the control group was associated with impaired motor behavior even when conditions were predictable. Similarly, a part of the older group seemed to pre-potentiate its cortex lying beneath the cluster of “sensory” electrodes which was associated with more safe and accurate steps. Further investigations should focus on the importance of sensorimotor integration and its’ changes due to PD or healthy aging and beta ERD may be an excellent tool for this task

Adhesive wear and particle emission: Numerical approach based on asperity-free formulation of Rabinowicz criterion

In 1953 Archard formulated his general law of wear stating that the amount of worn material is proportional to the normal force and the sliding distance, and is inversely proportional to the hardness of the material. Five years later in 1958, Rabinowicz suggested a criterion determining the minimum size of wear particles. Both concepts became very popular due to their simplicity and robustness, but did not give thorough explanation of the mechanisms involved. It wasn’t until almost 60 years later in 2016 that Aghababaei, Warner and Molinari (AWM) used quasi-molecular simulations to confirm the Rabinowicz criterion. One of the central quantities remained the “asperity size”. Because real surfaces have roughness on many length scales, this size is often ill-defined. The present paper is devoted to two main points: First, we generalize the Rabinowicz-AWM criterion by introducing an “asperity-free” wear criterion, applicable even to fractal roughness. Second, we combine our generalized Rabinowicz criterion with the numerical contact mechanics of rough surfaces and formulate on this basis a deterministic wear model. We identify two types of wear: one leading to the formation of a modified topography which does not wear further and one showing continuously proceeding wear. In the latter case we observe regimes of least wear, mild wear and severe wear which have a clear microscopic interpretation. The worn volume in the region of mild wear occurs typically to be a power law of the normal force with an exponent not necessarily equal to one. The method provides the worn surface topography after an initial settling phase as well as the size distribution of wear particles. We analyse different laws of interface interaction and the corresponding wear laws. A comprehensive parameter study remains a task for future research.BMBF, 13NKE011A, Verbundprojekt: Reifenabrieb in der Umwelt (RAU) - Teilvorhaben: Einflussfaktoren, Mengen, Vorkommen und Maßnahme

The Combinatorial Fusion Cascade to Generate the Standard Genetic Code

Combinatorial fusion cascade was proposed as a transition stage between prebiotic chemistry and early forms of life. The combinatorial fusion cascade consists of three stages: eight initial complimentary pairs of amino acids, four protocodes, and the standard genetic code. The initial complimentary pairs and the protocodes are divided into dominant and recessive entities. The transitions between these stages obey the same combinatorial fusion rules for all amino acids. The combinatorial fusion cascade mathematically describes the codon assignments in the standard genetic code. It explains the availability of amino acids with the even and odd numbers of codons, the appearance of stop codons, inclusion of novel canonical amino acids, exceptional high numbers of codons for amino acids arginine, leucine, and serine, and the temporal order of amino acid inclusion into the genetic code. The temporal order of amino acids within the cascade is congruent with the consensus temporal order previously derived from the similarities between the available hypotheses. The control over the combinatorial fusion cascades would open the road for a novel technology to develop artificial microorganism

Plastic and tribological properties of polytetrafluoroethylene (PTFE) under conditions of high pressure and shear

We investigate experimentally the behavior of a thin sheet of polytetrafluoroethylene between a steel plate and a cylindrical steel indenter under combined action of high normal force and torsion. Under these actions, the polytetrafluoroethylene layer is partially squeezed out of the contact area. The thickness of the remaining layer is studied as function of the applied normal force, the torsion angle, and the radius of the indenter. We suggest a simple semi-empirical material model which describes both the process of squeezing out of the layer and the force of friction produced by the layer

Partial-slip frictional response of rough surfaces

If two elastic bodies with rough surfaces are first pressed against each other and then loaded tangentially, sliding will occur at the boundary of the contact area while the inner parts may still stick. With increasing tangential force, the sliding parts will expand while the sticking parts shrink and finally vanish. In this paper, we study the fractions of the contact area, tangential force and tangential stiffness, associated with the sticking portion of the contact area, as a function of the total applied tangential force up to the onset of full sliding. For the numerical analysis randomly rough, fractal surfaces are used, with the Hurst exponent H ranging from 0.1 to 0.9. Numerical simulations by boundary element method are compared with an analytical analysis in the framework of the Greenwood and Williamson (GW) model. In both cases, a universal linear dependency between the real contact area fraction in stick condition and the applied tangential force is found, regardless of the Hurst exponent of the rough surfaces. Regarding the dependence of the differential tangential stiffness on the tangential force, a linear relation is found in the GW case. For randomly rough surfaces, a nonlinear relation depending on H is derived

Combinatorial Fusion Rules to Describe Codon Assignment in the Standard Genetic Code

We propose combinatorial fusion rules that describe the codon assignment in the standard genetic code simply and uniformly for all canonical amino acids. These rules become obvious if the origin of the standard genetic code is considered as a result of a fusion of four protocodes: Two dominant AU and GC protocodes and two recessive AU and GC protocodes. The biochemical meaning of the fusion rules consists of retaining the complementarity between cognate codons of the small hydrophobic amino acids and large charged or polar amino acids within the protocodes. The proto tRNAs were assembled in form of two kissing hairpins with 9-base and 10-base loops in the case of dominant protocodes and two 9-base loops in the case of recessive protocodes. The fusion rules reveal the connection between the stop codons, the non-canonical amino acids, pyrrolysine and selenocysteine, and deviations in the translation of mitochondria. Using fusion rules, we predicted the existence of additional amino acids that are essential for the development of the standard genetic code. The validity of the proposed partition of the genetic code into dominant and recessive protocodes is considered referring to state-of-the-art hypotheses. The formation of two aminoacyl-tRNA synthetase classes is compatible with four-protocode partition