Alkane-grown Beauveria bassiana produce mycelial pellets displaying peroxisome proliferation, oxidative stress, and cell surface alterations

The entomopathogenic fungus Beauveria bassiana is able to grow on insect cuticle18 hydrocarbons as the sole carbon source, inducing several enzymes involved in alkane19 assimilation and concomitantly increasing virulence against insect hosts. In this study, we20 describe some physiological and molecular processes implicated in growth, nutritional21 stress response, and cellular alterations found in alkane-grown fungi. The fungal cytology22 was investigated using light and transmission electron microscopy (TEM) while the surface23 topography was examined using atomic force microscopy (AFM). Fungal hydrophobicity24 was also measured on the cell surface. Additionally, the expression pattern of several genes25 associated with oxidative stress, peroxisome biogenesis, and hydrophobicity were analysed26 by qPCR. We found a novel type of growth in alkane-cultured B. bassiana similar to27 mycelial pellets described in other alkane-free fungi, which were able to germinate and28 produce viable conidia in media without a carbon source and to be pathogenic against29 larvae of the beetles Tenebrio molitor and Tribolium castaneum. Optical microscopy and30 TEM showed that pellets were formed by hyphae cumulates with high peroxidase activity,31 exhibiting peroxisome proliferation and an apparent surface thickening. Alkane-grown32 conidia appeared to be more hydrophobic and cell surfaces displayed different topography33 than glucose-grown cells, as it was observed by AFM. We also found a significant34 induction in several genes encoding for peroxins, catalases, superoxide dismutases, and35 hydrophobins. These results show that both morphological and metabolic changes are36 triggered in mycelial pellets derived from alkane-grown B. bassiana.Fil: Huarte Bonnet, Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; ArgentinaFil: Santos Da Paixao, Flavia Regina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; ArgentinaFil: Ponce, Juan C. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; ArgentinaFil: Santana, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; ArgentinaFil: Prieto, Eduardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Pedrini, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; Argentin

Factors Associated with Normal-Weight Abdominal Obesity Phenotype in a Representative Sample of the Peruvian Population: A 4-Year Pooled Cross-Sectional Study

To examine factors associated with abdominal obesity among normal-weight individuals from the Demographic and Health Survey of Peru (2018–2021). Cross-sectional analytical study. The outcome variable was abdominal obesity defined according to JIS criteria. Crude (cPR) and adjusted prevalence ratios (aPR) were estimated for the association between sociodemographic and health-related variables and abdominal obesity using the GLM Poisson distribution with robust variance estimates. A total of 32,109 subjects were included. The prevalence of abdominal obesity was 26.7%. The multivariate analysis showed a statistically significant association between abdominal obesity and female sex (aPR: 11.16; 95% CI 10.43–11.94); categorized age 35 to 59 (aPR: 1.71; 95% CI 1.65–1.78); 60 to 69 (aPR: 1.91; 95% CI 1.81–2.02); and 70 or older(aPR: 1.99; 95% CI 1.87–2.10); survey year 2019 (aPR: 1.22; 95% CI 1.15–1.28); 2020 (aPR: 1.17; 95% CI 1.11–1.24); and 2021 (aPR: 1.12; 95% CI 1.06–1.18); living in Andean region (aPR: 0.91; 95% CI 0.86–0.95); wealth index poor (aPR: 1.26; 95% CI 1.18–1.35); middle (aPR: 1.17; 95% CI 1.08–1.26); rich (aPR: 1.26; 95% CI 1.17–1.36); and richest (aPR: 1.25; 95% CI 1.16–1.36); depressive symptoms (aPR: 0.95; 95% CI 0.92–0.98); history of hypertension (aPR: 1.08; 95% CI 1.03–1.13), type 2 diabetes (aPR: 1.13; 95% CI 1.07–1.20); and fruit intake 3 or more servings/day (aPR: 0.92; 95% CI 0.89–0.96). Female sex, older ages, and low and high income levels increased the prevalence ratio for abdominal obesity, while depressive symptoms

Phenomenology of the SU(3)(c) x SU(3)(L) x U(1)(X) model with exotic charged leptons

A phenomenological analysis of the three-family model based on the local gauge group SU(3)c⊗SU(3)L⊗U(1)X with exotic charged leptons, is carried out. Instead of using the minimal scalar sector able to break the symmetry in a proper way, we introduce an alternative set of four Higgs scalar triplets, which combined with an anomaly-free discrete symmetry, produce quark and charged lepton mass spectrum without hierarchies in the Yukawa coupling constants. We also embed the structure into a simple group and show some conditions to achieve a low gauge coupling unification, avoiding possible conflict with proton decay bounds. By using experimental results from the CERN-LEP, SLAC linear collider, and atomic parity violation data, we update constraints on several parameters of the model

Cu(I)‑Catalyzed Asymmetric [3 + 2] Cycloaddition of Azomethine 2 Ylides with Cyclobutenones

Al texto del artículo le acompaña el material complementarioThe catalytic asymmetric 1,3-dipolar cycloaddition of cyclobutenones with azomethine ylides provides straightforward access to densely substituted 3-azabicyclo[3.2.0]heptanes. In the presence of CuI/(R)-Fesulphos as the catalytic system, high levels of diastereoselectivity and enantioselectivity were achieved (up to 98% enantiomeric excess (ee)).Financial support of this work by the Ministerio de Economía, Industria y Competitividad and Fondo Europeo de Desarrollo Regional (Project Nos. CTQ2012-35790 MINECO and CTQ2015-66954-P MINECO/FEDER, UE) is gratefully acknowledged. A.P. thanks the MICINN for a predoctoral fellowship. J.C. thanks the MECD for a FPU predoctoral fellowshi

Alkane-grown <i>Beauveria bassiana</i> produce mycelial pellets displaying peroxisome proliferation, oxidative stress, and cell surface alterations

The entomopathogenic fungus Beauveria bassiana is able to grow on insect cuticle hydrocarbons as the sole carbon source, inducing several enzymes involved in alkane assimilation and concomitantly increasing virulence against insect hosts. In this study, we describe some physiological and molecular processes implicated in growth, nutritional stress response, and cellular alterations found in alkane-grown fungi. The fungal cytology was investigated using light and transmission electron microscopy (TEM) while the surface topography was examined using atomic force microscopy (AFM). Fungal hydrophobicity was also measured on the cell surface. Additionally, the expression pattern of several genes associated with oxidative stress, peroxisome biogenesis, and hydrophobicity were analysed by qPCR. We found a novel type of growth in alkane-cultured B. bassiana similar to mycelial pellets described in other alkane-free fungi, which were able to germinate and produce viable conidia in media without a carbon source and to be pathogenic against larvae of the beetles Tenebrio molitor and Tribolium castaneum. Optical microscopy and TEM showed that pellets were formed by hyphae cumulates with high peroxidase activity, exhibiting peroxisome proliferation and an apparent surface thickening. Alkane-grown conidia appeared to be more hydrophobic and cell surfaces displayed different topography than glucose-grown cells, as it was observed by AFM. We also found a significant induction in several genes encoding for peroxins, catalases, superoxide dismutases, and hydrophobins. These results show that both morphological and metabolic changes are triggered in mycelial pellets derived from alkane-grown B. bassiana.Instituto de Investigaciones Bioquímicas de La PlataInstituto de Investigaciones Fisicoquímicas Teóricas y Aplicada

DIGITAL DIVIDE IN PERUVIAN HIGHER EDUCATION: A POST-PANDEMIC REVIEW

By early 2020, the COVID-19 pandemic paralyzed the world, with the origin being the Chinese city of Wuhan. By March 11, 2020, the World Health Organization (WHO) declared COVID-19 a pandemic.  The dire consequences of this disease were reflected in a historic global recession, and in the health field, SARS-CoV-2 had wreaked havoc, especially in the elderly, due to worse manifestations and higher mortality rates. As a result, most countries were able to curb the spread of the virus by imposing mandatory measures such as not leaving their homes and very strict timetables. Among the measures considered was the closure of educational institutions, including universities. At that time, there was a paradigm shift in the way educators had to change the way they taught classes by making use of various online platforms. Online, distance and continuing education learning became a panacea for this unprecedented global pandemic for both educators and students. In this review, a passage is made through the different scenarios experienced by the COVID-19 pandemic, taking into account several objectives such as: the COVID-19, and the case of universities, the educational transition: from face-to-face to virtuality, challenges and opportunities after the pandemic, and last but not least, how the Peruvian State, was able to cope with the pandemic of Covid-19, and the challenges that should continue to be considered after the pandemic

Combined theoretical and spectroscopic Raman study of 3,4-ethylenedioxy and S,S-dioxide substituted terthiophenes and their parent polymers

Abstract This paper reports on the analysis of the Raman spectra, supported by DFT quantum chemical calculations, of a series of terthiophenes which present the novelty of being selectively functionalized with sulfone groups to increase their photoluminescence properties. The spectra have been carefully assigned on the basis of the theoretical vibrational eigenvectors. They suggest the occurrence of a segmentation of the electronic structure which can become a crucial feature for optimizing linear and non linear optical properties. Sulfonation fixes the two lone electron pairs of the sulfur atom, in particular that involved in the aromatization of the thiophene ring, thus coexisting two different kind of five member (aromatic and non aromatic) units in the same chain. This duality of the structure gives rise to the splitting of the characteristic Raman lines associated to the CaC/C-C stretching modes into components mainly located either on the external rings or on the internal one. The study also focuses on the electrosynthesized polymers obtained upon electropolymerization of the corresponding terthiophenes.

Plesiomonas shigelloides Infection, Ecuador, 2004–2008

Diarrheal risk associated with Plesiomonas shigelloides infection was assessed in rural communities in northwestern Ecuador during 2004–2008. We found little evidence that single infection with P. shigelloides is associated with diarrhea but stronger evidence that co-infection with rotavirus causes diarrhea

Emotional Intelligence in Times of Covid-19: a Comparative Study in Peruvian Universities

Objective:The aim of the study was to compare emotional intelligence in Peruvian university students from public and private universities.Theoretical framework: In higher education, emotionally intelligent students have the ability to interact appropriately with others, achieve good academic results, and reach personal and professional goals.Method: The methodology was descriptive-comparative, the sample consisted of 838 university students of both sexes from public (471) and private (364) universities belonging to the coast, highlands and jungle regions of Peru. A sociodemographic form created for the study and the emotional intelligence scale (TMMS-24) were used as measurement instruments.Results and conclusion:The results showed that university students over 30 years of age, women, those from the highlands and those who worked had high levels of emotional intelligence.Implications of the research:It is necessary for university institutions to reorganize their academic conditions and provide the necessary inputs to apply socioemotional strategies to students. Researchers linked to the lines of research on emotional intelligence should develop predictive studies to know which factors may be predicting adequate or inadequate emotional intelligence in university students.Originality:This study demonstrates that it is essential to attend to and provide emotional intelligence tools to those students whodo not have the necessary resources to face different situations in the face of the COVID-19 pandemic and contributes to the training of educators, especially environmental educators in crisis contexts