Large step down voltage converters for desalination

One percent of the world's drinking water is currently desalinated, and this will have to increase to 14% by 2025. Desalination is energy intensive, having significant commercial and ecological implications. One of the most promising methods of desalination is capacitive deionisation which only uses 1kWh/m3 but requires a voltage of less than 1.8V at currents of up to 1000A This thesis produced hardware capable of creating 550A at a voltage of 1.8V, giving over a 1kW power rating, with an input voltage of 340V dc. The converter designed was a bidirectional asymmetrical half-bridge flyback converter allowing for isolation at these high step down ratios. The converter was used to charge a bank of 17,000F supercapacitors from 0V to 1.8V, with an initial charging step down ratio in excess of 340:1 falling to 190:1 as the load charged. A novel Asymmetrical Half-Bridge Coupled-Inductor Buck converter is presented as the ideal solution for large step-down ratios with analysis comparing the ability to efficiently step down a voltage with other common converters, the buck and flyback converters. A comparison between a single-ended coupled-inductor buck converter employing a buck-boost voltage clamp and the novel asymmetrical half-bridge coupled-inductor buck converter circuit shows that the asymmetrical half-bridge converter is a more efficient circuit as leakage energy is recovered; the switch voltages are clamped to within the dc voltage rating of the bridge and the control strategy is simple. Passive and active snubbers are reviewed for efficiency, switch ratings and management of the effects of leakage inductance and compared against the novel designs presented. In the desalination application isolation is required so the flyback circuit is used. An isolated three switch bidirectional converter is constructed using silicon carbide MOSFETs and diodes switching at 40kHz. The converter uses novel current measuring techniques, an on-board microprocessor and closed loop control designed into the final DC-DC converter.One percent of the world's drinking water is currently desalinated, and this will have to increase to 14% by 2025. Desalination is energy intensive, having significant commercial and ecological implications. One of the most promising methods of desalination is capacitive deionisation which only uses 1kWh/m3 but requires a voltage of less than 1.8V at currents of up to 1000A This thesis produced hardware capable of creating 550A at a voltage of 1.8V, giving over a 1kW power rating, with an input voltage of 340V dc. The converter designed was a bidirectional asymmetrical half-bridge flyback converter allowing for isolation at these high step down ratios. The converter was used to charge a bank of 17,000F supercapacitors from 0V to 1.8V, with an initial charging step down ratio in excess of 340:1 falling to 190:1 as the load charged. A novel Asymmetrical Half-Bridge Coupled-Inductor Buck converter is presented as the ideal solution for large step-down ratios with analysis comparing the ability to efficiently step down a voltage with other common converters, the buck and flyback converters. A comparison between a single-ended coupled-inductor buck converter employing a buck-boost voltage clamp and the novel asymmetrical half-bridge coupled-inductor buck converter circuit shows that the asymmetrical half-bridge converter is a more efficient circuit as leakage energy is recovered; the switch voltages are clamped to within the dc voltage rating of the bridge and the control strategy is simple. Passive and active snubbers are reviewed for efficiency, switch ratings and management of the effects of leakage inductance and compared against the novel designs presented. In the desalination application isolation is required so the flyback circuit is used. An isolated three switch bidirectional converter is constructed using silicon carbide MOSFETs and diodes switching at 40kHz. The converter uses novel current measuring techniques, an on-board microprocessor and closed loop control designed into the final DC-DC converter

Investigations into the function of structural isoforms of the platelet derived growth factor A-chain

Platelet derived growth factor (PDGF) is a covalent dimer of A and B chains with the structure AB, AA or BB depending on its source. The A and B chains are related but distinct polypeptides encoded by separate genes. PDGF-A can be differentially spliced, allowing production of two mature proteins one of which (PDGF-AL) is longer than the other (PDGF-AS) by 15 amino acids at its carboxyl terminus. PDGF-A homodimers are thought to be important in early development and, later, for regulating glial cell development in the central nervous system (CNS). In order to gain a clearer understanding of the role of PDGF in vivo it is important to define the functional significance of the different isoforms and their cellular sources and targets. This thesis describes my attempts to elucidate the functional differences between PDGF-AS and PDGF-AL and to determine whether they are differentially expressed in CNS derived tissues. To investigate the functional differences between PDGF-AS and PDGF-AL I introduced plasmid vectors directing the expression of the two isoforms into cultured Cos cells and subjected the expressed molecules to biochemical and immunocytochemical analysis. These studies provide evidence that PDGF-AS is secreted as a freely diffusible molecule while PDGF-AL is sequestered near the cells that produce it by binding to heparin-like molecules on the cell surface and in the extracellular matrix (ECM). The implications of these findings are discussed with reference to other factors that exist in different isoforms that are either freely diffusible or immobilised at the cell surface and within the ECM. I attempted to analyse the expression pattern in the CNS of the two isoforms by a combination of techniques, including SI nuclease protection, PCR amplification on reverse transcribed RNA, cDNA cloning and sequencing. These studies suggest that PDGF AS predominates in the tissues and cell types I have examined in agreement with other studies of a similar nature

BioSAVE: display of scored annotation within a sequence context.

BACKGROUND: Visualization of sequence annotation is a common feature in many bioinformatics tools. For many applications it is desirable to restrict the display of such annotation according to a score cutoff, as biological interpretation can be difficult in the presence of the entire data. Unfortunately, many visualisation solutions are somewhat static in the way they handle such score cutoffs. RESULTS: We present BioSAVE, a sequence annotation viewer with on-the-fly selection of visualisation thresholds for each feature. BioSAVE is a versatile OS X program for visual display of scored features (annotation) within a sequence context. The program reads sequence and additional supplementary annotation data (e.g., position weight matrix matches, conservation scores, structural domains) from a variety of commonly used file formats and displays them graphically. Onscreen controls then allow for live customisation of these graphics, including on-the-fly selection of visualisation thresholds for each feature. CONCLUSION: Possible applications of the program include display of transcription factor binding sites in a genomic context or the visualisation of structural domain assignments in protein sequences and many more. The dynamic visualisation of these annotations is useful, e.g., for the determination of cutoff values of predicted features to match experimental data. Program, source code and exemplary files are freely available at the BioSAVE homepage.RIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are

Mixing ratios of trace gases in the austral polar atmosphere during August and September of 1987

Mixing ratios are presented for a number of long-lived trace gases in the austral polar atmosphere during August and September of 1987. The recent discovery of a 12-year trend of increasing depletion of ozone over the Antarctic Continent in the spring of each year led to numerous theoretical interpretations and several scientific expeditions to the region. The results herein were obtained as part of a major effort involving penetration of the region of ozone depletion by NASA's multi-instrumented aircraft. One of the 14 instruments on the high-altitude ER-2 aircraft collected pressurized air samples between latitudes of 53 degrees and 72 degrees south at pressure altitudes up to 21 km in a series of 12 flights from Punta Arenas, Chile, over the Palmer Peninsula. The sampling system, located in the nose section of ER-2, has an inlet tube in the free airstream, a metal-bellows air pump, and 14 specially treated 1.6 l stainless-steel canisters for containing the pressurized air at 350 kPa. A typical flight profile consisted of a southbound path on the 428 K potential temperature surface, a descent to a pressure altitude of 13.7 km, a climb to the 460 K surface, and return on this surface. Mixing ratios for the trace gases were obtained from gas chromatographic analyses of the pressurized air samples. Of the species measured, the mixing ratios for CH4, CO, N2O, CF2 Cl2, CFCl3, CH3, CCl3, CCl4, and C2F3Cl3 are reported here

Voltage Clamping Circuits for Large Voltage Step-Down Coupled Inductor Converters

Water desalination processes require power converters with large voltage step-down ratios and high efficiencies.  Coupled‑inductor (tapped-inductor) circuits can achieve this where the whole inductor carries current during one switch state but only part of the inductor carries current during the second switch state. The switch state change results in excessive switch voltages due coupling leakage. To protect the switching device, a novel voltage clamp is presented which improves the overall efficiency of the switching circuit and recovers otherwise wasted energy. It requires no closed-loop control. For higher power levels, an asymmetric half-bridge coupled-inductor buck converter circuit, which offers switch protection without a snubber, is presented. The circuit is shown to offer reduced switching losses despite having two switching components in the high-voltage forward conduction path. </p

Sheep scab spatial distribution: the roles of transmission pathways

Abstract Background Ovine psoroptic mange (sheep scab) is a highly pathogenic contagious infection caused by the mite Psoroptes ovis. Following 21 years in which scab was eradicated in the UK, it was inadvertently reintroduced in 1972 and, despite the implementation of a range of control methods, its prevalence increased steadily thereafter. Recent reports of resistance to macrocyclic lactone treatments may further exacerbate control problems. A better understanding of the factors that facilitate its transmission are required to allow improved management of this disease. Transmission of infection occurs within and between contiguous sheep farms via infected sheep-to-sheep or sheep–environment contact and through long-distance movements of infected sheep, such as through markets. Methods A stochastic metapopulation model was used to investigate the impact of different transmission routes on the spatial pattern of outbreaks. A range of model scenarios were considered following the initial infection of a cluster of highly connected contiguous farms. Results Scab spreads between clusters of neighbouring contiguous farms after introduction but when long-distance movements are excluded, infection then self-limits spatially at boundaries where farm connectivity is low. Inclusion of long-distance movements is required to generate the national patterns of disease spread observed. Conclusions Preventing the movement of scab infested sheep through sales and markets is essential for any national management programme. If effective movement control can be implemented, regional control in geographic areas where farm densities are high would allow more focussed cost-effective scab management. Graphical Abstrac

Assessing the effectiveness of prophylactic treatment strategies for sheep scab

Ovine psoroptic mange (sheep scab) is a condition caused by a hypersensitivity response to the ectoparasitic mite, Psoroptes ovis. It is an animal welfare concern and causes extensive economic losses to the sheep industry worldwide. More effective scab management is required to limit increases in infection prevalence, particularly given growing concerns over acaricide resistance. Here, a stochastic metapopulation model is used to explore the effectiveness of a range of prophylactic acaricide treatment strategies in comparison to no intervention. Over a simulated one-year period, movement control, based on the prophylactic treatment of animals being moved in sales, followed by farm biosecurity of bought in animals, was shown to be the most effective at reducing scab risk and more cost-effective than no intervention. Localised targeting of prophylaxis in areas of high scab prevalence was more effective than using prophylaxis at random, however, this localised effect declined post-treatment because of the import of infected animals. The analysis highlights the role of the movement of infected animals in maintaining high levels of scab infection and the importance of reducing this route of transmission to allow localised management to be effective