Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera base on its whole-genome analysis

The insecticidal proteins of Bacillus thuringiensis are used as formulations of spore-crystal complexes and their genes have been incorporated into several crops, which has provided a model for genetic engineering in agriculture. Despite the variability of the Cry proteins described so far, it is still necessary to look for toxins with a broad spectrum of action, since a significant number of pests are not controlled with the available Cry proteins. It is also important to provide alternatives to address the problem of insect resistance, which has already appeared with the use of formulations and in transgenic plants that express cry genes that code for insecticidal proteins. We report the characterization of a novel B. thuringiensis isolate native to Argentina (FCC7) toxic against lepidoptera and coleoptera insects. The strain shows a rounded crystal harboring mainly a protein of about 130 kDa. Through the whole-genome sequencing by Illumina Miseq 1500 platform we detected two crystal protein genes with cry8-like genes homology, three vegetative insecticidal protein (Vip) genes and multiple virulence factors such as phospholipases, proteases, enhancins, chitinases, among others. The two cry8-like genes, homologous to the sequences Cry8Ac1 and Cry8Qa1 with 73,4 % and 88,9 % identity respectively, were cloned and expressed into the 4Q7/pSTAB system and the larvicidal activity were tested against Spodoptera frugiperda and Tenebrio molitor. Two of the Vip genes were identified as Vip1-like with an identity of 74,9% and 70,3% respectively while the third Vip gene was 82% identical to Vip2 sequences.Fil: Lazarte, Jorge Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; ArgentinaFil: Poliero, Aimará Ayelen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; ArgentinaFil: Berón, Corina Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; ArgentinaInternational Congress on Invertebrate Pathology and Microbial Control & 52nd Annual Meeting of the Society for Invertebrate Pathology & 17th Meeting of the IOBC‐WPRS Working Group "Microbial and Nematode Control of Invertebrate Pests"ValenciaEspañaSocietyfor Invertebrate PathologyOrganisation of IOB

Honeys from the Argentine Phytogeographic Provinces Chaqueña and Monte in Catamarca and La Rioja

The characterization of Argentinean honeys from the vegetation units (VUs) of the phytogeographical provinces located in Catamarca and La Rioja was performed with the analysis used for honey quality control: sugar profile, free acidity, pH, electrical conductivity (EC), color and contents of moisture, ash, total soluble solids, and hydroxymethylfurfural (HMF). Honeys were authentic and traceable, and complied with the specifications of national and/or international standards, verifying their blossom origin, high quality, good maturity, and freshness. Honeys from VU-9 and VU-23 presented significantly distinctive physicochemical parameters, evidencing the influence of the flora and pedoclimatic conditions of these VUs. Honeys from VU-23 presented significantly higher contents of glucose and total reducing sugars (F + G), and lower EC, ash contents, fructose/glucose ratio, and concentrations of turanose and maltose than those from VU-9. Honeys from VU-23 in La Rioja also exhibited characteristic lighter colors and lower pH and ºBrix values and turanose amounts; and honeys from VU-9 in La Rioja exhibited highest pH values. Catamarca honeys were characterized by high free acidity and high amounts of turanose, regardless of the VUs. The contents of sugars and total soluble solids, moisture, pH, EC, and color of honeys from Catamarca and La Rioja are published here for the first time. This study contributes to the typification of honeys from these provinces, which will provide them an added value and allow them to access newer markets.Fil: Poliero, Aimará Ayelen. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química. Grupo de Investigación en Ingeniería en Alimentos; ArgentinaFil: Aubone, Ines. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Amadei Enghelmayer, Marisa. Nexco, S.a.; ArgentinaFil: Rosso, Valeria Soledad. Nexco, S.a.; ArgentinaFil: Fuselli, Sandra Rosa. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones en Sanidad Producción y Ambiente. - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Instituto de Investigaciones en Sanidad Producción y Ambiente; ArgentinaFil: Alonso Salces, Rosa Maria. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentin

Physicochemical Characterization and Sugar Profile of Argentinian Honeys From the Phytogeographic Provinces Paranaense and Pampeana in Misiones

The characterization of honeys from the Biomes and vegetation units of the Phytogeographical Provinces (PP) located in the Province of Misiones (Argentina) was performed by analysis of sugar profile and physicochemical parameters (Free Acidity, pH, Electrical Conductivity (EC), colour and contents of moisture, ash, total soluble solids and hydroxymethylfurfural) used for quality control of honey. The honeys analysed were authentic and traceable, and met the specifications of the national and/or international standards for the evaluated parameters, denoting blossom origin and confirming high quality, maturity and freshness. The influence of biome and Vegetation Units (VU) on physical and chemical parameters of the studied honeys was defined regarding both flora and pedoclimatic conditions. Honeys from VU-27 presented significantly higher contents of fructose, ash and total soluble solids, pH and EC and lower moisture content than those from VU-3. To the authors’ best knowledge, sugar profiles, free acidity and contents of ash and total soluble solids in honeys from the different PPs, vegetation units and Biomes of Misiones are reported for the first time. This study contributes to the classification of honeys from Misiones, helping to increase added value and access new markets.Fil: Poliero, Aimará Ayelen. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; ArgentinaFil: Aubone, Ines. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; ArgentinaFil: Amadei Enghelmayer, Marisa. No especifíca;Fil: Rosso, Valeria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Müller, P. F.. No especifíca;Fil: Fuselli, Sandra Rosa. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; ArgentinaFil: Alonso Salces, Rosa Maria. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentin

Characterization of Argentinian Honeys Based on their Sugar Profiles and Quality Parameters

The honeys from the Argentinian provinces of Buenos Aires, La Rioja,Catamarca and Misiones were characterised by the sugar profile (fructose, glucose,sucrose, turanose, maltose and erlose) and the physicochemical parameters (freeacidity, pH, electrical conductivity (EC), colour and contents of moisture, ash,total soluble solids and hydroxymethylfurfural (HMF)) used for honey qualitycontrol. Authentic and traceable honey samples (n = 572) collected along fiveharvests were analysed using the official analytical methods. All honeys met thespecifications of the national and/or international standards for the evaluatedparameters, which denoted the blossom origin of most honeys, and confirmedtheir high quality, good maturity and freshness. The influence of the flora andthe pedoclimatic conditions of each phytogeographical region on the physicaland chemical properties of honey allowed its characterization. Thus, BuenosAires produced typical lighter honeys, and Misiones and Catamarca, darker ones.Buenos Aires honeys presented particular lower values of pH, EC, ash, HMF andmaltose, and higher concentrations of erlose. Misiones honeys exhibited highervalues of moisture, free acidity, EC and turanose amounts, and lower contentsof fructose, glucose and total soluble solids. La Rioja honeys showed higherpH and ºBrix values; and Catamarca honeys, higher sucrose contents. To theauthors? knowledge, the sugar profiles and several quality parameters of honeysfrom Misiones and Catamarca are here reported for the first time, as well as anyphysical and chemical data on La Rioja honeys.Fil: Poliero, Aimará Ayelen. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; ArgentinaFil: Aubone, Ines. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; ArgentinaFil: Amadei Enghelmayer, Marisa. No especifíca;Fil: Rosso, Valeria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Müller, Pablo Fernando. Instituto Nuestra Señora de Itatí. Profesorado en Ciencias Agrarias y Protección Ambiental; ArgentinaFil: Fuselli, Sandra Rosa. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; ArgentinaFil: Alonso Salces, Rosa Maria. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; Argentin

Valutazione della stabilità dell'ollio d'oliva vergine in conservazione mediante profilo 1H-NMR e chemiometria

La qualità dell'ollio vergine di oliva (VOO) è correlata alla sue proprietà sensoriali e nutrizionali, alla stabilità ossidativa e alla sua conservazione. L'ossidazione del VOO porta alla formazione di sostanze sgradevoli, alla degradazione dei suoi antiossidanti bioattivi e all'accumulo di composti di degradazione, causando la persita delle sua qualità sensoriali e salutari, del suo valore economico e dell'accettazione da parte dei consumatori. La resistenza del VOO all'ossidazione dipende dalla sua composizione chimica e dalla sua esposizione a fattori pro-ossidante come ossigeno, luce e temperatura. La stabilità del VOO durante la conservazione è stata studiata mediante un approccio metabolomico non mirato basato sull'impronta digitale 1H-NMR e sul riconoscimento del modello che simula le normali condizioni di conservazione durante la sua commercializzazione.Fil: Alonso Salces, Rosa Maria. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaFil: Gallo, Blanca. Universidad del País Vasco; EspañaFil: Poliero, Aimará Ayelen. Universidad Nacional de Mar del Plata; ArgentinaFil: Viacava, Gabriela Elena. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química. Grupo de Investigación en Ingeniería en Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaFil: García González, Diego Luis. Instituto de la Grasa; España. Consejo Superior de Investigaciones Científicas; EspañaFil: Gallina Toschi, Tullia. Universidad de Bologna; ItaliaFil: Servili, Maurizio. Università di Perugia; ItaliaFil: Berrueta, Luis Angel. Universidad del País Vasco; Españ

Stepwise strategy based on 1H-NMR fingerprinting in combination with chemometrics to determine the content of vegetable oils in olive oil mixtures

1H NMR fingerprinting of edible oils and a set of multivariate classification and regression models organised in a decision tree is proposed as a stepwise strategy to assure the authenticity and traceability of olive oils and their declared blends with other vegetable oils (VOs). Oils of the 'virgin olive oil' and 'olive oil' categories and their mixtures with the most common VOs, i.e. sunflower, high oleic sunflower, hazelnut, avocado, soybean, corn, refined palm olein and desterolized high oleic sunflower oils, were studied. Partial least squares (PLS) discriminant analysis provided stable and robust binary classification models to identify the olive oil type and the VO in the blend. PLS regression afforded models with excellent precisions and acceptable accuracies to determine the percentage of VO in the mixture. The satisfactory performance of this approach, tested with blind samples, confirm its potential to support regulations and control bodies. Keywords: Adulteration; Authentication; Decision tree; Multivariate data analysis; Nuclear magnetic resonance; Olive oil

Determination of Vegetable Oils in Olive Oil Mixtures Using a Stepwise Strategy Based on H-1-Nmr Fingerprinting and Pattern Recognition

Final version is available in open access in publisher’s site.-- This abstract is based on the published article: Rosa María Alonso-Salces, Luis Ángel Berrueta, Beatriz Quintanilla-Casas, Stefania Vichi, Alba Tres, María Isabel Collado, Carlos Asensio-Regalado, Gabriela Elena Viacava, Aimará Ayelen Poliero, Enrico Valli, Alessandra Bendini, Tullia Gallina Toschi, José Manuel Martínez-Rivas, Wenceslao Moreda, Blanca Gallo. Stepwise strategy based on 1H-NMR fingerprinting in combination with chemometrics to determine the content of vegetable oils in olive oil mixtures. Food Chemistry, Volume 366, 2022, 130588, https://doi.org/10.1016/j.foodchem.2021.130588.--http://hdl.handle.net/10261/261907Peer reviewe

Molecular characterization of a new Bacillus thuringiensis strain from Argentina toxic against Lepidoptera and Coleoptera based on its whole-genome analysis

The insecticidal proteins of Bacillus thuringiensis are used as formulations of spore-crystal complexes and their genes have been incorporated into several crops, which has provided a model for genetic engineering in agriculture. Despite the variability of the Cry proteins described so far, it is still necessary to look for toxins with a broad spectrum of action, since a significant number of pests are not controlled with the available Cry proteins. It is also important to provide alternatives to address the problem of insect resistance, which has already appeared with the use of formulations and in transgenic plants that express cry genes that code for insecticidal proteins. We report the characterization of a novel B. thuringiensis isolate native to Argentina (FCC7) toxic against lepidoptera and coleoptera insects. The strain shows a rounded crystal harboring mainly a protein of about 130 kDa. Through the whole-genome sequencing by Illumina Miseq 1500 platform we detected two crystal protein genes with cry8-like genes homology, three vegetative insecticidal proteins (Vips) genes and multiple virulence factors such as phospholipases, proteases, enhancins, chitinases, among others. The two cry8-like genes, homologues of Cry8Ac1 and Cry8Qa1 sequences with 73,4 % and 88,9 % of identity respectively, were cloned and expressed into the 4Q7/pSTAB system and the larvicidal activity were assayed against Spodoptera frugiperda and Tenebrio molitor. Two of the Vips genes were identified as Vip1-like with 74,9% and 70,3% identity respectively while the third Vip gene was 82% identical to Vip2 sequences.Fil: Lazarte, Jorge Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; ArgentinaFil: Poliero, Aimará Ayelen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; ArgentinaFil: Berón, Corina Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; ArgentinaXIV Encuentro Biólog@s En Red: 14 años por una ciencia hecha entre todes y para todesMar del PlataArgentinaAsociación de Jóvenes Investigadores en Formació

Effect of climate change on the polyphenolic composition of the main varieties of grape from La Rioja (Spain) and new oenological strategies to correct these effects on the quality of red wine

Even though grapes grow all around the planet those aimed for high quality wine production occur in areas with very specific climate conditions. Moreover, each individual variety of grape requires conditions that are even more particular. Thus, any change on the climate or meteorological patterns can affect the chemistry of the grape and the final quality of wine. Recent studies have explored the impact of climate change on the worldwide production of grape, and some highlight an advance on the vintage date associated with a temperature increase, this being for instance, 18-21 days in the South of France from 1940 to 2000. Regarding the chemical composition of wine, anthocyanins and condensed tannins are the main type of polyphenolic compounds found in red wine. Anthocyanins are the main pigments of red grapes whereas tannins play an important role on the astringency and bitterness of wine. During wine making process, both types of compounds undergo chemical transformations leading to more stable polymeric pigments. The mechanisms of these reactions and the structures of these derivative pigments have been a recurrent topic of study. With the advances on the analytical techniques, some of these structures have been elucidated, discovering products of the direct and indirect condensation of anthocyanins and tannins. Additionally, anthocyanins have been found to react with other minor components present in wine, yielding a new class of compounds called pyranoanthocyanins and producing changes in the hue of the wine [3]. A better knowledge of the formation kinetics and evolution of these pigments during wine production will allow the producer to adapt the oenological practice to control and optimise the final quality of red wine. This work is aimed towards the study of climate influence on the polyphenolic composition of must and wine from Tempranillo grape cultivar, the main variety of grape in La Rioja (Spain), and its evolution during wine production.Fil: Asensio-Regalado, Carlos. Universidad del País Vasco; EspañaFil: Sasía-Arriba, Andrea. Universidad del País Vasco; EspañaFil: Poliero, Aimará Ayelen. Universidad del País Vasco; EspañaFil: Alonso Salces, Rosa Maria. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; Argentina. Universidad Nacional de Mar del Plata, Facultad de Ciencias Exactas y Naturales, Centro de Investigación en Abejas Sociales (CIAS); ArgentinaFil: Gallo, Blanca. Universidad del País Vasco; EspañaFil: Berrueta, Luis Ángel. Universidad del País Vasco; EspañaXXXth International Conference on PolyphenolsTurkuFinlandiaGroupe Polyphénols and University of Turk

Untargeted metabolomic LC-MS fingerprinting of apple cultivars for the identification of biomarkers related to resistance to rosy apple aphid

Phenolic compounds may play important roles in plant responses to biotic-stress and in resistance mechanisms. In a previous work, we demonstrated the correlation between phenolic composition of apple cultivars and their tolerance to rosy apple aphid (RAA), Dysaphis plantaginea, a key pest of apple. The study was performed on a full-sib family produced at SERIDA, derived from a crossing between the local apple cultivar ?Meana? and the resistant cultivar ?Florina?. It consisted in the determination of the concentrations of individual flavan 3 ols, hydroxycinnamic acids, dihydrochalcones and flavonols by UHPLC-DAD present in the two parents and 154 descendants; and their resistance to RAA was determined after aphid inoculation in greenhouse. A relationship between the concentration of three caffeoylquinic acid isomers (4-CQA, 1-CQA and 3-CQA) and 4-p-coumaroylquinic acid (4-pCoQA) and the resistance to RAA was found by pattern recognition techniques. Cultivar resistance to RAA was concluded to be related with the phenylpropanoid pathway; the isomerisations being the key metabolic reactions for a cultivar to be resistant or susceptible to RAA.In the present work, the phenolic profiles of the same set of samples were analysed by UHPLC-ESI(+)-QTOF/MS to use an untargeted metabolomic approach to identify biomarkers related to resistance to RAA. Apple phenolic fingerprints and their resistance to RAA were submitted to multivariate data analysis by Principal Component Analysis, Hierarchical Clustering Analysis, Partial Least Square-Discriminant Analysis (PLSDA) and Orthogonal-PLSDA (O-PLSDA), which disclosed that the main features related to resistant cultivars were due to 4-CQA, and those related to susceptible cultivars to 5-pCoQA. These results agreed with previous ones since both phenolics are involved in the isomerisation reactions mentioned above.Fil: Sasía Arriba, Andrea. Universidad del País Vasco; EspañaFil: Alonso Salces, Rosa Maria. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; ArgentinaFil: Asensio Regalado, Carlos. Universidad del País Vasco; EspañaFil: Poliero, Aimará Ayelen. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; ArgentinaFil: Abad García, Beatriz. Universidad del País Vasco; EspañaFil: Dapena, Enrique. No especifíca;Fil: Gallo, Blanca. Universidad del País Vasco; EspañaFil: Berrueta, Luis Ángel. Universidad del País Vasco; EspañaXXX International Conference on PolyphenolsTurkuFinlandiaInternational Association of Groupe PolyphenolsUniversity of Turk