Bypass of DNA interstrand crosslinks by a Rev1-DNA polymerase ζ complex

DNA polymerase ζ (Pol ζ) and Rev1 are essential for the repair of DNA interstrand crosslink (ICL) damage. We have used yeast DNA polymerases η, ζ and Rev1 to study translesion synthesis (TLS) past a nitrogen mustard-based interstrand crosslink (ICL) with an 8-atom linker between the crosslinked bases. The Rev1-Pol ζ complex was most efficient in complete bypass synthesis, by 2-3 fold, compared to Pol ζ alone or Pol η. Rev1 protein, but not its catalytic activity, was required for efficient TLS. A dCMP residue was faithfully inserted across the ICL-G by Pol η, Pol ζ, and Rev1-Pol ζ. Rev1-Pol ζ, and particularly Pol ζ alone showed a tendency to stall before the ICL, whereas Pol η stalled just after insertion across the ICL. The stalling of Pol η directly past the ICL is attributed to its autoinhibitory activity, caused by elongation of the short ICL-unhooked oligonucleotide (a six-mer in our study) by Pol η providing a barrier to further elongation of the correct primer. No stalling by Rev1-Pol ζ directly past the ICL was observed, suggesting that the proposed function of Pol ζ as an extender DNA polymerase is also required for ICL repair

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[Review of] Paul M. Sniderman and Thomas Piazza. The Scar of Race

Into the murky, politically-charged waters of contemporary racial politics shines this welcome ray of light. Paul M. Sniderman and Thomas Piazza, using clever research design and innovative techniques, clarify the changing meaning of race in today’s political landscape and conclusively dismiss many strongly-held, but nonetheless inaccurate, assumptions about whites’ attitudes toward African Americans

Ressenyes

Index de les obres ressenyades: Josep M. CATALÀ DOMÈNECH, La imagen interfaz: Representación audiovisual y conocimiento en la era de la complejida

Facharztweiterbildung Psychiatrie und Psychotherapie: Problemorientiertes Lernen - Evaluation eines Modellprojekts

Zusammenfassung: Die Betonung individueller Lernbedürfnisse, der Fähigkeit zur Lösung komplexer klinischer Probleme sowie einer von interkollegialer Kommunikation geprägten professionellen Grundhaltung durch das problemorientierte Lernen (POL) spricht für dessen Eignung als didaktisches Format in der Facharztweiterbildung. Dennoch wurde es bisher selten hierfür eingesetzt. Im Rahmen dieses Modellprojektes wurde das POL in das Kurrikulum der strukturierten Facharztweiterbildung Psychiatrie und Psychotherapie aufgenommen und über einen Zeitraum von 12Monaten mittels strukturierter Fragebögen evaluiert. Es fanden im Evaluationszeitraum 41POL-Kurse statt, an denen insgesamt 447 Assistenzärzte teilnahmen. Die Teilnehmer und die Tutoren bewerteten 19 von 21 erfragten Aspekten der POL-Kurse als gut bis sehr gut (Mittelwert auf einer 5-stufigen Likert-Skala >4). Insgesamt wurde das POL als besonders geeignet für die Weiterbildung eingeschätzt (Teilnehmer 4,5±0,8; Tutoren 5,0±0,2). Die Ergebnisse dieses Modellprojekts sprechen für die Eignung des POL als Teil eines vielfältigen Weiterbildungsangebots, um den Praxisbezug und die Anwendbarkeit des Wissens im klinischen Alltag zu stärke

Isolation of TFIIIC102 in Arabidopsis thaliana to better understand transcription

Abstract only availableIn eukaryotes, transcription is carried out by three RNA polymerases. RNA polymerase I (Pol I) transcribes genes encoding the large rRNAs, RNA Pol II transcribes genes encoding proteins involved in mRNA processing, and RNA Pol III transcribes genes encoding tRNA and 5S rRNA. Each polymerase consists of a number of subunits that work together to regulate transcription through various stages of cell growth and development. While polymerase subunit interactions have been widely studied in human and yeast models, experiments concerning the polymerase subunit interactions in plant models have barely begun to scratch the surface. Defining the proteins and DNA sequences responsible for plant tRNA gene expression and determining how tRNA gene expression is regulated during plant growth and development would be highly beneficial to the scientific community. Breaking down the components of Pol III has provided the foundation for a better understanding of plant transcription factors. This polymerase consists of the Pol III molecule and transcription factors TFIIIA, TFIIIB, and TFIIIC. Pol III, and all its components, participate in many stages of transmission of cellular genetic information, including transcription, translation, and RNA processing. Here, a look at TFIIIC protein interactions shall provide some insight to the methods and regulation of transcription over the course of much long-term research. TFIIIC consists of six subunits that all work together to carry out the proper transcription of genetic material. Of the six individual subunits, TFIIIC102 consumed the majority of the research performed in the lab over the last eight weeks.Plant Genomics Internships @ M

Two Political Sciences Or One? Liberal Arts Political Science As A Disciplinary Partner

This essay sketches the ways that the practices of political science at top R1 institutions and at leading liberal arts colleges differ. But one also can see the two practices as partners in a common enterprise of making political science rigorous, relevant, and clear. This article outlines such a collaboration