Nanotecnologia e gravura em metal: o trabalho colaborativo no laboratório científico

This text is part of the postdoctoral research, developed in PPG in Pharmaceutical Sciences of UFRGS, in 2018, with funding from CNPq. The main goal of the research is to use nanotechnology knowledge to characterize, plan, develop and produce alternative non-toxic materials for use in the printmaking, such as varnishes and intaglio inks. We analyze the types, profile and size of particles present in the varnishes, to improve the alternative materials in order to obtain a differentiated technical and artistic response. We intend to offer new bases for the realization of the images made through the intaglio etching using new technologies from the production of new materials and also the comprehension (in nanoscales) of what we perceive intuitively through the graphic expression in the intaglio etching and printing lines, grooves, points and other incisions produced on the engraving plates.Este texto faz parte da pesquisa de pós-doutorado, desenvolvida no PPG em Ciências Farmacêuticas da UFRGS, em 2018, com financiamento do CNPq. O objetivo principal da pesquisa é utilizar os conhecimentos da área da nanotecnologia para caracterizar, planejar, desenvolver e produzir materiais alternativos não-tóxicos para uso na gravura, tais como vernizes de proteção e tintas de impressão. Analisamos os tipos e perfis de distribuição de tamanhos de partículas, que estão presentes nos vernizes, para aprimorar os materiais alternativos de modo a obter uma resposta técnica e artística diferenciada. Pretendemos oferecer novas bases para a realização das imagens realizadas através das gravuras com uso de novas tecnologias a partir da produção de novos materiais e também da compreensão (em nanoescala) daquilo que percebemos intuitivamente através da expressão gráfica nas gravações e impressões de linhas, ranhuras, pontos e demais incisões realizadas nas matrizes de metal

Lipid core nanocapsules-loaded tacrolimus: Development and evaluation of quality parameters

This study aimed to revalidate an HPLC-based analytical methodology to determine  tacrolimus within lipid-core nanocapsules and to evaluate the quality of such nanosystems. Chromatographic separation was achieved by employing a C18 column as a stationary phase and a ternary mixture of acetonitrile: water: phosphoric acid (700:299:1 v/v) as the mobile phase. The revalidated method proved to be linear in the range of 1-60 µg.mL−1 for tacrolimus (r2 >0.999). Detection and quantification limits were 45.38 ng.mL-1 and 137.51 ng.mL-1, respectively, which assures the methodology sensitivity. The method was also precise (RSD = 1.78% between samples). Besides, the methodology demonstrated accuracy and robustness. The lipid-core nanocapsules-loaded tacrolimus showed exclusively nanosized particles (±190 nm and polydispersity index of ≤v0.2), negative zeta potential (-13.67±1.16), and slightly acidic pH (5.58 ± 0.06), with a content of 98.90±2.32%  and encapsulation rate of 99.23±0.32%.  Tacrolimus-loaded in lipid-core nanocapsules-loaded tacrolimus showed stability for at least 30 days at room temperature and a sustained release profile compared to the drug in solution