Brum, Tainá Lenz de

Guterres, Silvia Stanisçuaski

Pohlmann, Adriana Raffin

English

Lume 5.8

ISSN 0102-6593 caderno do farmácia Órgão Oficial da Faculdade de Farmácia da Universidade Federal do Rio Grande do Sul volume 26, Suplemento, 2010 TECNOLOGIA FARMACÊUTICA E BIOFARMÁCIA f''"),-- ()_$ S _$ Pharmaceutical technology and biopharmacy 228-------------------------=----------------------------~----~--~ DEVELOPMENT OF RHO-B LOADED NANOCAPSULES ANO MICROCAPSULES TO EVALUATE THE EFFECT OF SIZE IN THE EXV/VOSKIN PENETRATION Brum T. 1:; Guterres 8. 1; Pohlmann A. 1·2 1Laboratório de Cosmetologia, Faculdade de Farmácia, UFRGS; 2Laboratório K204, Instituto de Química, UFRGS; *Mestranda - Início: 2009/1. lntroduction: Polymeric nanoparticles, such as nanocapsules, are submicrometric devices (10-1 000 nm) in which an oily-core is surrounded by a polymeric wall. Their main advantages include the control of the drug release and the drug protection against physical or chemical degradation 1. Microparticulated carriers have been developed to modify the pharmacokinetic profiles of drugs, to protect unstable molecules from degradation and to reduce the drug toxicitf. In the past years, different strategies have been proposed to increase or to control the skin permeation and to circumvent inadequate physicochemical characteristics of several substances3 . Objective: The aim of the present work is to study the relationship between the ex vivo skin permeation of rhodamine B (RHO-B), used as a model target core, encapsulated in nanocapsules (NC) and microcapsules (MC), and the particle sizes distribution using different pig skin conditions: intact, waxed, intact irradiated with UVA and waxed irradiated with UVA. Materiais and Methods: Two formulations of poly(e-caprolactone) were prepared by different methods: the MC1 suspension by emulsification-solvent diffusion4 and the NC2 by interfacial deposition of preformed polymer5 . A mixture of the capric/caprylic triglycerides was used to form the particles oily-core. The formulations were first analyzed by laser diffraction (LD) to verify the size scale distribution, mean size and polydispersity (SPAN). For the formulation which presented size in nanometric scale (NC2), measures were taken for the particle size distribution, average diameter and polydispersityindex (PDI) using photon correlation spectroscopy (PCS) as this technique is more specific to the nanometer range. The electrophoretic potential of the particles surface (zeta potential) were estimated by electrophoretic mobility. A potentiometer was used to determine the pH values of the suspensions. Results and Discussion: The results of LD showed a monomodal distribution of particle size, with a mean size of 7 1-1m for MC1 (Fig. 1.a red) and of 192 nm for NC2 (Fig. 1.a green). The SPAN values were lower than 1 ,80 for both systems. By PCS, NC2 presented average diameter and PDI values of 187 nm and 0.08, respectively (Fig. 1.b). The pH values were close to 5. The value of zeta potential was negative for both formulations (-9,28 mV for MC1 and -25,60 mV for NC2). Conclusions: The results demonstrated that both formulations presented satisfactory values in the physicochemical characterization. Therefore, the next step is the onset of skin permeation expe rime nts. (a) :!I I (b) ~::L. i I I ~ I "/" I I (. ~ I ~ 10 // ~ i! . ~ ' .-- o '' .. 1 ' ' ' ' ' I ' ' ' ' ' I ~ 01 o 1 10 100 1000 3000 01 1 10 100 1000 10000 Partide S1ze (um) Slle(dnmi Fig. 1. Size distribuition profile of (a) NC2 and MC1 and (b) NC2. References: 1. E. Jãger et a/., J.Biomed.Nanotechnol. 5, 130 (2009). 2. L. Cruz etal., Eur. J. Pharm. Sei., doi:10.1016/j.ejps.2010.05.001 (2010). 3. S. Guterres et ai., Drug Target lnsights, 2, 147 (2007). 4. D. Quintanar-Guerrero et a/., Pharm Res., 15, 1056 (1998). 5. H. Fessi c lnt. J. Pharm., 55, R1-R4 (1989). Acknowldgements: Financiai support from master scholarship from CAPES/Brazil. 

Development of RHO-B loaded nanocapsules and microcapsules to evaluate the effect of size in the ex vivo skin penetration

https://lume.ufrgs.br/bitstream/10183/267773/1/000770555.pdf

Development of RHO-B loaded nanocapsules and microcapsules to evaluate the effect of size in the ex vivo skin penetration

Abstract

Similar works

Full text

Available Versions

Lume 5.8