1,232 research outputs found
Selection of DNA nanoparticles with preferential binding to aggregated protein target.
High affinity and specificity are considered essential for affinity reagents and molecularly-targeted therapeutics, such as monoclonal antibodies. However, life's own molecular and cellular machinery consists of lower affinity, highly multivalent interactions that are metastable, but easily reversible or displaceable. With this inspiration, we have developed a DNA-based reagent platform that uses massive avidity to achieve stable, but reversible specific recognition of polyvalent targets. We have previously selected these DNA reagents, termed DeNAno, against various cells and now we demonstrate that DeNAno specific for protein targets can also be selected. DeNAno were selected against streptavidin-, rituximab- and bevacizumab-coated beads. Binding was stable for weeks and unaffected by the presence of soluble target proteins, yet readily competed by natural or synthetic ligands of the target proteins. Thus DeNAno particles are a novel biomolecular recognition agent whose orthogonal use of avidity over affinity results in uniquely stable yet reversible binding interactions
The role of aerodynamic forces in a mathematical model for suspension bridges
In a fish-bone model for suspension bridges studied by us in a previous paper
we introduce linear aerodynamic forces. We numerically analyze the role of
these forces and we theoretically show that they do not influence the onset of
torsional oscillations. This suggests a new explanation for the origin of
instability in suspension bridges: it is a combined interaction between
structural nonlinearity and aerodynamics and it follows a precise pattern. This
gives an answer to a long-standing question about the origin of torsional
instability in suspension bridges
Repair of a Reinforced Earth Wall
The facing of a Reinforced Earth retaining wall, built at an altitude of 1200 m, was damaged during the winter 1981. The repair was achieved quickly and under traffic. The instrumentation carried out on the repairs and the tests run on the backfill material have revealed the action of the frost and its increase in the fortuitous presence of water
Tuning gaps and phases of a two-subband system in a quantizing magnetic field
In this work we study the properties of a two-subband quasi-two-dimensional
electron system in a strong magnetic field when the electron filling factor is
equal to four. When the cyclotron energy is close to the intersubband splitting
the system can be mapped onto a four-level electron system with an effective
filling factor of two. The ground state is either a ferromagnetic state or a
spin-singlet state, depending on the values of the inter-level splitting and
Zeeman energy. The boundaries between these phases are strongly influenced by
the inter-electron interaction. A significant exchange-mediated enhancement of
the excitation gap results in the suppression of the electron-phonon
interaction. The rate of absorption of non-equilibrium phonons is calculated as
a function of Zeeman energy and inter-subband splitting. The phonon absorption
rate has two peaks as a function of intersubband splitting and has a step-like
structure as a function of Zeeman energy
Quasiparticle properties of a coupled quantum wire electron-phonon system
We study leading-order many-body effects of longitudinal optical (LO) phonons
on electronic properties of one-dimensional quantum wire systems. We calculate
the quasiparticle properties of a weakly polar one dimensional electron gas in
the presence of both electron-phonon and electron-electron interactions. The
leading-order dynamical screening approximation (GW approximation) is used to
obtain the electron self-energy, the quasiparticle spectral function, and the
quasiparticle damping rate in our calculation by treating electrons and phonons
on an equal footing. Our theory includes effects (within the random phase
approximation) of Fermi statistics, Landau damping, plasmon-phonon mode
coupling, phonon renormalization, dynamical screening, and impurity scattering.
In general, electron-electron and electron-phonon many-body renormalization
effects are found to be nonmultiplicative and nonadditive in our theoretical
results for quasiparticle properties.Comment: 21 pages, Revtex, 12 figures enclose
Three-dimensional pattern formation, multiple homogeneous soft modes, and nonlinear dielectric electroconvection
Patterns forming spontaneously in extended, three-dimensional, dissipative
systems are likely to excite several homogeneous soft modes (
hydrodynamic modes) of the underlying physical system, much more than quasi
one- and two-dimensional patterns are. The reason is the lack of damping
boundaries. This paper compares two analytic techniques to derive the patten
dynamics from hydrodynamics, which are usually equivalent but lead to different
results when applied to multiple homogeneous soft modes. Dielectric
electroconvection in nematic liquid crystals is introduced as a model for
three-dimensional pattern formation. The 3D pattern dynamics including soft
modes are derived. For slabs of large but finite thickness the description is
reduced further to a two-dimensional one. It is argued that the range of
validity of 2D descriptions is limited to a very small region above threshold.
The transition from 2D to 3D pattern dynamics is discussed. Experimentally
testable predictions for the stable range of ideal patterns and the electric
Nusselt numbers are made. For most results analytic approximations in terms of
material parameters are given.Comment: 29 pages, 2 figure
Electron-phonon scattering at the intersection of two Landau levels
We predict a double-resonant feature in the magnetic field dependence of the
phonon-mediated longitudinal conductivity of a two-subband
quasi-two-dimensional electron system in a quantizing magnetic field. The two
sharp peaks in appear when the energy separation between two
Landau levels belonging to different size-quantization subbands is favorable
for acoustic-phonon transitions. One-phonon and two-phonon mechanisms of
electron conductivity are calculated and mutually compared. The phonon-mediated
interaction between the intersecting Landau levels is considered and no avoided
crossing is found at thermal equilibrium.Comment: 13 pages, 8 figure
Modulated structures in electroconvection in nematic liquid crystals
Motivated by experiments in electroconvection in nematic liquid crystals with
homeotropic alignment we study the coupled amplitude equations describing the
formation of a stationary roll pattern in the presence of a weakly-damped mode
that breaks isotropy. The equations can be generalized to describe the planarly
aligned case if the orienting effect of the boundaries is small, which can be
achieved by a destabilizing magnetic field. The slow mode represents the
in-plane director at the center of the cell. The simplest uniform states are
normal rolls which may undergo a pitchfork bifurcation to abnormal rolls with a
misaligned in-plane director.We present a new class of defect-free solutions
with spatial modulations perpendicular to the rolls. In a parameter range where
the zig-zag instability is not relevant these solutions are stable attractors,
as observed in experiments. We also present two-dimensionally modulated states
with and without defects which result from the destabilization of the
one-dimensionally modulated structures. Finally, for no (or very small)
damping, and away from the rotationally symmetric case, we find static chevrons
made up of a periodic arrangement of defect chains (or bands of defects)
separating homogeneous regions of oblique rolls with very small amplitude.
These states may provide a model for a class of poorly understood stationary
structures observed in various highly-conducting materials ("prechevrons" or
"broad domains").Comment: 13 pages, 13 figure
Subsurface structure of Planum Boreum from Mars Reconnaissance Orbiter Shallow Radar soundings
We map the subsurface structure of Planum Boreum using sounding data from the Shallow Radar (SHARAD) instrument onboard the Mars Reconnaissance Orbiter. Radar coverage throughout the 1,000,000- km2 area reveals widespread reflections from basal and internal interfaces of the north polar layered deposits (NPLD). A dome-shaped zone of diffuse reflectivity up to 12 ls (1-km thick) underlies twothirds of the NPLD, predominantly in the main lobe but also extending into the Gemina Lingula lobe across Chasma Boreale. We equate this zone with a basal unit identified in image data as Amazonian sand-rich layered deposits [Byrne, S., Murray, B.C., 2002. J. Geophys. Res. 107, 5044, 12 pp. doi:10.1029/2001JE001615; Fishbaugh, K.E., Head, J.W., 2005. Icarus 174, 444â474; Tanaka, K.L., Rodriguez, J.A.P., Skinner, J.A., Bourke, M.C., Fortezzo, C.M., Herkenhoff, K.E., Kolb, E.J., Okubo, C.H., 2008. Icarus 196, 318â358]. Elsewhere, the NPLD base is remarkably flat-lying and co-planar with the exposed surface of the surrounding Vastitas Borealis materials. Within the NPLD, we delineate and map four units based on the radar-layer packets of Phillips et al. [Phillips, R.J., and 26 colleagues, 2008. Science 320, 1182â 1185] that extend throughout the deposits and a fifth unit confined to eastern Gemina Lingula. We estimate the volume of each internal unit and of the entire NPLD stack (821,000 km3), exclusive of the basal unit. Correlation of these units to models of insolation cycles and polar deposition [Laskar, J., Levrard, B., Mustard, J.F., 2002. Nature 419, 375â377; Levrard, B., Forget, F., Montmessin, F., Laskar, J., 2007. J. Geophys. Res. 112, E06012, 18 pp. doi:10.1029/2006JE002772] is consistent with the 4.2-Ma age of the oldest preserved NPLD obtained by Levrard et al. [Levrard, B., Forget, F., Montmessin, F., Laskar, J., 2007. J. Geophys. Res. 112, E06012, 18 pp. doi:10.1029/2006JE002772]. We suggest a dominant layering mechanism of dustâcontent variation during accumulation rather than one of lag production during periods of sublimation
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