112 research outputs found
The embryonic node functions as an instructive stem cell niche
In warm-blooded vertebrate embryos (mammals and birds), the body forms from a growth zone at the tail end. Hensen’s node, a region which induces and patterns the neural axis is located within this growth zone. The node also contains the precursors of neural, mesodermal and endodermal structures along the midline and has been suggested to contain a small population of resident stem cells. However, it is unknown whether the rest of the node constitutes an instructive stem cell niche, specifying stem cell behaviour. Here we combine transplantation of a single cell in vivo with single-cell mRNA sequencing in the chick and show that when made to enter the node, non-node-progenitor cells become resident and gain stem cell behaviour. These cells preferentially express G2/M phase cell-cycle related genes and are concentrated in posterior sub-regions of the node. The posterior part of the node therefore behaves as an instructive stem cell niche. These results demonstrate a new function for the vertebrate node during development
Sustainable, “green” solutions for concrete pavement rehabilitation - a feasibility (pilot) study
Development of ultrasound detection in American shad (Alosa sapidissima)
It has recently been shown that a few fish species, including American shad (Alosa sapidissima; Clupeiformes), are able to detect sound up to 180 kHz, an ability not found in most other fishes. Initially, it was proposed that ultrasound detection in shad involves the auditory bullae, swim bladder extensions found in all members of the Clupeiformes. However, while all clupeiformes have bullae, not all can detect ultrasound. Thus, the bullae alone are not sufficient to explain ultrasound detection. In this study, we used a developmental approach to determine when ultrasound detection begins and how the ability to detect ultrasound changes with ontogeny in American shad. We then compared changes in auditory function with morphological development to identify structures that are potentially responsible for ultrasound detection. We found that the auditory bullae and all three auditory end organs are present well before fish show ultrasound detection behaviourally and we suggest that an additional specialization in the utricle (one of the auditory end organs) forms coincident with the onset of ultrasound detection. We further show that this utricular specialization is found in two clupeiform species that can detect ultrasound but not in two clupeiform species not capable of ultrasound detection. Thus, it appears that ultrasound-detecting clupeiformes have undergone structural modification of the utricle that allows detection of ultrasonic stimulation
The nuclear lamina couples mechanical forces to cell fate in the preimplantation embryo via actin organization
During preimplantation development, contractile forces generated at the apical cortex segregate cells into inner and outer positions of the embryo, establishing the inner cell mass (ICM) and trophectoderm. To which extent these forces influence ICM-trophectoderm fate remains unresolved. Here, we found that the nuclear lamina is coupled to the cortex via an F-actin meshwork in mouse and human embryos. Actomyosin contractility increases during development, upregulating Lamin-A levels, but upon internalization cells lose their apical cortex and downregulate Lamin-A. Low Lamin-A shifts the localization of actin nucleators from nucleus to cytoplasm increasing cytoplasmic F-actin abundance. This results in stabilization of Amot, Yap phosphorylation and acquisition of ICM over trophectoderm fate. By contrast, in outer cells, Lamin-A levels increase with contractility. This prevents Yap phosphorylation enabling Cdx2 to specify the trophectoderm. Thus, forces transmitted to the nuclear lamina control actin organization to differentially regulate the factors specifying lineage identity
Solitary Esophageal Varix Simulating a Neoplasm
Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/73070/1/j.1440-1673.1988.tb02785.x.pd
Cryogenic Technology Development for Exploration Missions
This paper reports the status and findings of different cryogenic technology research projects in support of the President s Vision for Space Exploration. The exploration systems architecture study is reviewed for cryogenic fluid management needs. It is shown that the exploration architecture is reliant on the cryogenic propellants of liquid hydrogen, liquid oxygen and liquid methane. Needs identified include: the key technologies of liquid acquisition devices, passive thermal and pressure control, low gravity mass gauging, prototype pressure vessel demonstration, active thermal control; as well as feed system testing, and Cryogenic Fluid Management integrated system demonstration. Then five NASA technology projects are reviewed to show how these needs are being addressed by technology research. Projects reviewed include: In-Space Cryogenic Propellant Depot; Experimentation for the Maturation of Deep Space Cryogenic Refueling Technology; Cryogenic Propellant Operations Demonstrator; Zero Boil-Off Technology Experiment; and Propulsion and Cryogenic Advanced Development. Advances are found in the areas of liquid acquisition of liquid oxygen, mass gauging of liquid oxygen via radio frequency techniques, computational modeling of thermal and pressure control, broad area cooling thermal control strategies, flight experiments for resolving low gravity issues of cryogenic fluid management. Promising results are also seen for Joule-Thomson pressure control devices in liquid oxygen and liquid methane and liquid acquisition of methane, although these findings are still preliminary
Liquid Nitrogen Testing of ISRU Liquefaction Methods in Unsteady Applications
To enable NASAs planned long duration missions, the agency is putting emphasis on reusable cryogenic systems. Such systems will require replenishing of cryogens on-orbit via a cryogenic tanker or refueling depot, and potentially on the lunar or Martian surfaces with the utilization of in-situ resources. Surface replenishing requires the in-situ production of gaseous oxygen (and hydrogen if on the lunar surface), followed by liquefaction and storage. Funded by NASAs Advanced Exploration Systems, and managed under the Advanced Cis-Lunar Space Capability Project, the Cryogenic Fluid In-Situ Liquefaction for Landers (CryoFILL) multi center team was formed to develop a liquefaction and storage system that is efficient, reliable and scalable. This presentation will demonstrate the liquefaction and storage of In-Situ like propellant via a Tube-On-Tank Heat Exchanger integrated with Active Cooling (cryocooler) (verify proof of concept and obtain relevant data for model validation) and gather lessons learned from brassboard testing which will be applied to future liquefaction system prototype testing, then eventually to an end-to-end demonstration
The 894G>T variant in the endothelial nitric oxide synthase gene and spina bifida risk
The 894G>T single nucleotide polymorphism (SNP) in the endothelial NOS (NOS3) gene, has recently been associated with embryonic spina bifida risk. In this study, a possible association between the NOS3 894G>T SNP and spina bifida risk in both mothers and children in a Dutch population was examined using both a case-control design and a transmission disequilibrium test (TDT). Possible interactions between the NOS3 894G>T SNP and the MTHFR 677C>T SNP, elevated plasma homocysteine, and decreased plasma folate concentrations were also studied. The NOS3 894TT genotype did not increase spina bifida risk in mothers or children (OR 1.50, 95%CI 0.71–3.19 and OR 1.78, 95%CI 0.75–4.25, respectively). The TDT demonstrated no preferential transmission of the NOS3 894T allele (Χ2 = 0.06, P = 0.81). In combination with the MTHFR 677TT genotype or elevated plasma homocysteine concentrations, the NOS3 894GT/TT genotype increased maternal spina bifida risk (OR 4.52, 95%CI 1.55–13.22 and OR 3.38, 95%CI 1.46–7.84, respectively). In our study population, the NOS3 894GT/TT genotype might be a risk factor for having a spina bifida affected child in mothers who already have an impaired homocysteine metabolism
Differentiation of neurons from neural precursors generated in floating spheres from embryonic stem cells
<p>Abstract</p> <p>Background</p> <p>Neural differentiation of embryonic stem (ES) cells is usually achieved by induction of ectoderm in embryoid bodies followed by the enrichment of neuronal progenitors using a variety of factors. Obtaining reproducible percentages of neural cells is difficult and the methods are time consuming.</p> <p>Results</p> <p>Neural progenitors were produced from murine ES cells by a combination of nonadherent conditions and serum starvation. Conversion to neural progenitors was accompanied by downregulation of <it>Oct4 </it>and <it>NANOG </it>and increased expression of <it>nestin</it>. ES cells containing a GFP gene under the control of the <it>Sox1 </it>regulatory regions became fluorescent upon differentiation to neural progenitors, and ES cells with a tau-GFP fusion protein became fluorescent upon further differentiation to neurons. Neurons produced from these cells upregulated mature neuronal markers, or differentiated to glial and oligodendrocyte fates. The neurons gave rise to action potentials that could be recorded after application of fixed currents.</p> <p>Conclusion</p> <p>Neural progenitors were produced from murine ES cells by a novel method that induced neuroectoderm cells by a combination of nonadherent conditions and serum starvation, in contrast to the embryoid body method in which neuroectoderm cells must be selected after formation of all three germ layers.</p
Developmental axon pruning mediated by BDNF-p75NTR–dependent axon degeneration
The mechanisms that regulate the pruning of mammalian axons are just now being elucidated. Here, we describe a mechanism by which, during developmental sympathetic axon competition, winning axons secrete brain-derived neurotrophic factor (BDNF) in an activity-dependent fashion, which binds to the p75 neurotrophin receptor (p75NTR) on losing axons to cause their degeneration and, ultimately, axon pruning. Specifically, we found that pruning of rat and mouse sympathetic axons that project to the eye requires both activity-dependent BDNF and p75NTR. p75NTR and BDNF are also essential for activity-dependent axon pruning in culture, where they mediate pruning by directly causing axon degeneration. p75NTR, which is enriched in losing axons, causes axonal degeneration by suppressing TrkA-mediated signaling that is essential for axonal maintenance. These data provide a mechanism that explains how active axons can eliminate less-active, competing axons during developmental pruning by directly promoting p75NTR-mediated axonal degeneration
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