Study using microsatellites of main varieties of iberian pig

It is characterized a sample of 10 Iberian Pigs strains and one of Duroc with a panel of 25 microsatellites. It was detected a number of alleles between 4 and 20. The values of Heterocigosity by locus ranged between 0.03 for S0355 and 0.73 for S0068. The mean of q as genetic diversity measure was 0.12. After UPGMA tree representation from standard genetic distances, the strains Lampiño, Torbiscal were very well defined but Retinto Extremeño, Entrepelado and Silvela were not.Se caracteriza una muestra de 10 variedades de Cerdo Ibérico y una de Duroc con un panel de 25 microsatélites. Se detectó un número de alelos entre 4 y 20. Los valores de heterocigosidad por locus fluctuaron entre 0,03 para S0355 y 0,73 para S0068. La media del valor de q como medida de la diversidad genética fue de 0,12. Después de la representación de la distancia genética estándar usando el UPGMA algoritmo, las variedades Lampiño y Torbiscal se encontraron muy bien definidas, pero en el caso de las variedades Retinto extremeño, Entrepelado y Silvela no fue así

Ampliación de un portal Wordpress para el diseño de cursos basados en la metodología PBL

La metodología PBL propone el aprendizaje basado en problemas. A grandes rasgos sugiere que el estudiante sea el protagonista de su propio aprendizaje mediante el desarrollo de proyectos. Con esa idea, el grupo de innovación educativa DMAE-DIA (Desarrollo de nuevas Metodologías de Aprendizaje/Evaluación del Departamento de Informática Aplicada), comenzó la construcción de un portal web para mostrar los principios de dicha metodología, que permitiese a los usuarios del mismo conocerla y aprender a utilizarla en un entorno colaborativo. El objetivo de este trabajo es ampliar este portal para que los usuarios sean capaces de crear, eliminar y mantener sus propios proyectos para posteriormente utilizarlos como base para su práctica docente. El trabajo se ha planteado siguiendo el paradigma de la orientación a objetos, mediante la metodología UML y siguiendo el ciclo de vida del software y se ha implementado utilizando como base el CMS Wordpress y desarrollando con los lenguajes que más adelante se especificarán. Wordpress por su parte es un sistema de gestión de contenidos dotado de gran potencia que permite, de una manera muy sencilla, construir entornos web con el mínimo esfuerzo. ABSTRACT The PBL methodology proposed problem-based learning. Roughly suggests that the student is the protagonist of their own learning by developing projects. With that, the group of educational innovation DMAE-DIA (Development of new Learning/Assessment methodologies, Department of Applied Computing) began building a web portal to show the principles of this methodology, which would allow users to know and learn to use it in a collaborative environment. The aim of this work is to extend this website so that users are able to create, delete and maintain their own projects for later use as the basis for their teaching practice. The work has been raised following the paradigm of object orientation, by following the UML methodology and software life cycle and has been implemented using as a basis the CMS Wordpress and developed with the languages that will be specified later. Meanwhile Wordpress is a content management system endowed with great power that allows,in a very simple way to build web environments with minimal effort

Determination of toxic elements (mercury, cadmium, lead, tin and arsenic) in fish and shellfish samples. Risk assessment for the consumers

The authors would gratefully like to acknowledge the financial support given by Fondo de Investigaciones Sanitarias (reference PI10/00527). They are also grateful to the Spanish Ministry of Education, Culture and Sport for awarding Pablo Olmedo a FPU predoctoral fellowship (reference AP2009-0534) to achieve his PhD degree in the context of this research project.Although fish intake has potential health benefits, the presence of metal contamination in seafood has raised public health concerns. In this study, levels of mercury, cadmium, lead, tin and arsenic have been determined in fresh, canned and frozen fish and shellfish products and compared with the maximum levels currently in force. In a further step, potential human health risks for the consumers were assessed. A total of 485 samples of the 43 most frequently consumed fish and shellfish species in Andalusia (Southern Spain) were analyzed for their toxic elements content. High mercury concentrations were found in some predatory species (blue shark, cat shark, swordfish and tuna), although they were below the regulatory maximum levels. In the case of cadmium, bivalve mollusks such as canned clams and mussels presented higher concentrations than fish, but almost none of the samples analyzed exceeded the maximum levels. Lead concentrations were almost negligible with the exception of frozen common sole, which showed median levels above the legal limit. Tin levels in canned products were far below the maximum regulatory limit, indicating that no significant tin was transferred from the can. Arsenic concentrations were higher in crustaceans such as fresh and frozen shrimps. The risk assessment performed indicated that fish and shellfish products were safe for the average consumer, although a potential risk cannot be dismissed for regular or excessive consumers of particular fish species, such as tuna, swordfish, blue shark and cat shark (for mercury) and common sole (for lead).Instituto de Salud Carlos III PI10/00527Spanish Ministry of Education, Culture and Sport AP2009-053

From binge eating to binge drinking: A new and robust paradigm for assessing binge ethanol self-administration in male rats

Animal models of alcohol (ethanol) self-administration are crucial to dissect the neurobiological mechanisms underlying alcohol dependence, yet only a few of these induce pharmacologically relevant levels of alcohol consumption and rarely the alcohol self-administration co-occurs with other addictive behaviours. The present study aims to validate a novel model of voluntary ethanol consumption in male Wistar rats, in which ethanol access follows a binge eating experience. Over 10 sessions, Wistar rats were exposed to binge or control eating (i.e., the ingestion of 11.66 and 0.97 kcal/3 min, respectively, derived from a highly palatable food), immediately followed by two-bottle choice intake tests (2%, 6%, 10% or 14% w/w ethanol vs. water). Rats exposed to binge eating drank significantly more 6% or 10% (w/w) ethanol than control peers, reaching up to 6.3 gEtOH/kg. Rats stimulated with 2%, 6%, 10% or 14% ethanol after binge eating, but not those given those ethanol concentrations after control eating, exhibited significant within-group increases in ethanol drinking. This ethanol consumption was not altered by quinine adulteration (up to 0.1 g/L), and it was blocked by naltrexone (10 mg/kg), administered immediately before binge eating. Blood ethanol levels significantly correlated with ethanol consumption; and the more ethanol consumed, the greater the distance travelled in an open field test conducted after the two-bottle choice test. Altogether, this self-administration model seems a valid and robust alternative with remarkable potential for research on different stages of the alcohol addiction and, particularly, to assess interactions between alcohol consumption and others addictive-like behaviours.Junta de Andalucía, Grant/Award Numbers: CTS109, B-CTS-422-UGR18Ministerio de Universidades, Spain, Grant/Award Number: FPU18/05012Ministry of Science and Innovation, Grant/Award Number: MICIUPID2020- 114269GB-I00Spanish Ministry of Health (Government Delegation for the National Plan on Drugs), Grant/Award Number: PNSD 2020-04

Design and optimization of intake manifold in a Volkswagen car

The objective of this work was to develop a new design of a high performance intake manifold through a combination of CAD and FEM. First a FEA model was done, which included a complete thermal and structural analysis of the new intake manifold and the contact area between the aluminum coupling, using the combined tools of CATIA, ANSYS WORKBENCH, MATHCAD. Then several composite prototypes were made where analyzed.Martínez Sanz, AV.; Sanchez-Caballero, S.; Viu Betrán, A.; Pla-Ferrando, R. (2011). Design and optimization of intake manifold in a Volkswagen car. Annals of The University of Oradea. 2:29-34. http://hdl.handle.net/10251/35901S2934

TWIP/TRIP steels. Future trends in automotive industries

This paper aims to introduce a new type of steels which promise to have high relevance in future automotive structures. They will probably change the way the structural elements are now designed. First the most significant properties are described. Then environmental profits of its implantation are described. Finally the conclusions shows its suitability for a wide range of applications where an increase of safety with weight reduction are required.Pla-Ferrando, R.; Sanchez-Caballero, S.; Sellés Cantó, MÁ.; Martínez Sanz, AV. (2011). TWIP/TRIP steels. Future trends in automotive industries. Annals of The University of Oradea. 10(20):23-26. http://hdl.handle.net/10251/35908S2326102

Strategies to establish the structure and the evaluation system of the student projects in the bachelor engineering in industrial design and product development at Universitat Politècnica de València campus of Alcoi

[EN] The Bachelor Engineering in Industrial Design and Product Development is a multidisciplinary degree, in which a wide range of disciplines have a key role in the final projects developed by the students of the fourth grade. At Universitat Politècnica de València ¿ Campus d¿Alcoi, a number of lecturers are involved in an Education Project in order to change and adapt the evaluation system of the student projects. The current model is based on developing the work with the help of a supervisor and the evaluation carried out by other three teachers, who form the panel. In the frame of the Education Project, some teachers are trying to apply new strategies to improve both the supervising process and the evaluation system of the projects. First of all, an index is provided to the students to adapt the structure of their work. Then, it is intended that a number of teachers, each one from a different specialization, supervise the progress of the students weekly. Finally, the same teachers will take part in the evaluation as examiners. All this work must be integrated in the 120 attendance hours established to develop the project, which correspond to the 12 ECTS. In the frame of the new teaching-learning methodologies, the aim of this Education Project is to provide a system to develop and evaluate the student final projects, taking into account the role of students, supervisors and examiners in every stage of the work.Juliá Sanchis, E.; Jorda-Vilaplana, A.; Valor Valor, M.; Pla-Ferrando, L.; Sempere Ripoll, S.; Picó Silvestre, JF.; Bonet-Aracil, M.... (2017). Strategies to establish the structure and the evaluation system of the student projects in the bachelor engineering in industrial design and product development at Universitat Politècnica de València campus of Alcoi. EDULEARN Proceedings (Internet). 249-255. doi:10.21125/edulearn.2017.0106S24925

DEFINITION OF THE MAIN FEATURES OF THE STUDENT PROJECTS FOR THEIR DEVELOPMENT AND ASSESSMENT IN THE BACHELOR ENGINEERING IN INDUSTRIAL DESIGN AND PRODUCT DEVELOPMENT AT UNIVERSITAT POLITÈCNICA DE VALÈNCIA CAMPUS OF ALCOI

[EN] The Bachelor Engineering in Industrial Design and Product Development establishes the final project as a compulsory stage to achieve the degree. Due to the wide range of industrial products that can be designed, there is an extensive number of topics. The students can choose their final project according to the specialization they have studied all along the fourth academic year. At Universitat Politècnica de València ¿ Campus d¿Alcoi, three specializations can be studied: a) Product Design; b) Production and Innovation, and c) Textile and Fashion Design. Due to the multidisciplinary approach, there is a need to define a general index, both for students and lecturers, which contains the minimum contents of every type of project. Part of the work consists of analysing the three types of projects and the basic structure for each one, depending on the specialization. In addition, there is a particular project based on communication issues. A team of lecturers are involved in an education project at Universitat Politècnica de València. There is, at least, one representative for each specialization. The team has been working based on the last five years presented final projects and with a National Standard UNE 157001:2014: general criteria for the formal preparation of the documents constituting a technical project. The aim of this part of the project is to define the main structure for the minimum contents that these projects must include for their assessment. Finally, a document has been prepared as a basic guideline for students and lecturers, with definitions and instructions. Despite being four different topics, results show a common part which must be fulfilled by every project. Apart from the common part, every specialization has defined the particular features to be included. This document is useful for every part in the process: students to focus on the tasks to develop, lecturers to guide the students and introduce the process and the panel of teachers to obtain the basis for the evaluation.Bonet-Aracil, M.; Valor Valor, M.; Jorda-Vilaplana, A.; Sempere Ripoll, S.; Juliá Sanchis, E.; Pla-Ferrando, L.; Picó Silvestre, JF.... (2017). DEFINITION OF THE MAIN FEATURES OF THE STUDENT PROJECTS FOR THEIR DEVELOPMENT AND ASSESSMENT IN THE BACHELOR ENGINEERING IN INDUSTRIAL DESIGN AND PRODUCT DEVELOPMENT AT UNIVERSITAT POLITÈCNICA DE VALÈNCIA CAMPUS OF ALCOI. EDULEARN Proceedings (Internet). 9471-9474. doi:10.21125/edulearn.2017.0789S9471947

Genetic structure, relationships and admixture with wild relatives in native pig breeds from Iberia and its islands

Articles in International JournalsBackground: Native pig breeds in the Iberian Peninsula are broadly classified as belonging to either the Celtic or the Mediterranean breed groups, but there are other local populations that do not fit into any of these groups. Most of the native pig breeds in Iberia are in danger of extinction, and the assessment of their genetic diversity and population structure, relationships and possible admixture between breeds, and the appraisal of conservation alternatives are crucial to adopt appropriate management strategies. Methods: A panel of 24 microsatellite markers was used to genotype 844 animals representing the 17 most important native swine breeds and wild populations existing in Portugal and Spain and various statistical tools were applied to analyze the results. Results: Genetic diversity was high in the breeds studied, with an overall mean of 13.6 alleles per locus and an average expected heterozygosity of 0.80. Signs of genetic bottlenecks were observed in breeds with a small census size, and population substructure was present in some of the breeds with larger census sizes. Variability among breeds accounted for about 20% of the total genetic diversity, and was explained mostly by differences among the Celtic, Mediterranean and Basque breed groups, rather than by differences between domestic and wild pigs. Breeds clustered closely according to group, and proximity was detected between wild pigs and the Mediterranean cluster of breeds. Most breeds had their own structure and identity, with very little evidence of admixture, except for the Retinto and Entrepelado varieties of the Mediterranean group, which are very similar. Genetic influence of the identified breed clusters extends beyond the specific geographical areas across borders throughout the Iberian Peninsula, with a very sharp transition from one breed group to another. Analysis of conservation priorities confirms that the ranking of a breed for conservation depends on the emphasis placed on its contribution to the betweenand within-breed components of genetic diversity. Conclusions: Native pig breeds in Iberia reveal high levels of genetic diversity, a solid breed structure and a clear organization in well-defined clusters

Development of lateral flow immunoassay for rapid detection of oxytetracycline in honey samples

[EN] A lateral flow immunoassay (LFIA) was developed in the competitive reaction format and applied to test residues of the antimicrobial oxyteracycline (OTC) in honey samples. To prepare the assay test, a nitrocellulose membrane was stripped with hapten OTC3 conjugate to ovoalbumin egg protein (OVA-OTC3) and goat anti-rabbit antisera (GAR) as capture and control reagents, respectively. Polyclonal antisera against oxyteracycline was conjugated to colloidal gold nanoparticles and used as the detection reagent. The visual detection limit (cut-off value) of the oxyteracycline LFIA was 20 ng/g, and qualitative results were obtained within 10 min without expensive equipment. The assay was evaluated with OTC spiked honey samples from different geographical origin (n = 25). The results were in good agreement with those obtained from liquid chromatography separation and mass spectroscopy detection (LC-MS), indicating that the LFIA test might be used as a semiquantitative method for the determination of oxyteracycline. The system was also highly specific, showing no cross-reactivity to other chemically similar antibiotics.This work was supported by a grant from Ministry of Education and Science of Spain. CIT-060000-2007-57.Guillen Guille, I.; Martínez Sanmartín, Á.; Gabaldón Hernández, JA.; Núñez Delicado, E.; Puchades Pla, R.; Maquieira Catala, Á. (2011). Development of lateral flow immunoassay for rapid detection of oxytetracycline in honey samples. Food and Environment Safety. X(1):5-12. http://hdl.handle.net/10251/78761S512X