Evaluation of sustainable tourism potential of the principle Giant Mountains resorts in the Czech Republic

This paper proposes a new methodology for assessing the potential of sustainable tourism. It examines the overall potential of the landscape when faced with the negative impacts of tourism. Our assessment combines components of tourism and environmental sustainability. The methodology involved consultation with experts, and verification by tourists before being applied to the study area. The methodology was then applied to selected tourism centres in the Giant Mountains. The Giant Mountains are a popular tourist destination thanks to their outstanding natural beauty, and represent significant potential for tourism development. They are also one of the most over-burdened regions from tourism in the Czech Republic. However, many negative impacts of tourism exist, reducing the overall tourism potential of the region. Comparative results from the individual tourist centres in the study reveal the significant impact of potentially reducing attributes. Our assessment of the potential for sustainable tourism development in the area thus combines the environmental aspect of sustainable forms of tourism, with the identification of the most serious threats that need to be avoided to maintain the environment in the long-term. The results reveal the significant impact of excessive and inappropriate infrastructure and housing, as well as insufficient environmental education and legislation

Komplexní proteomické vyšetření infekčních a zánětlivých změn v pozdním předčasném prasknutí membrány před porodem

Preterm prelabour rupture of membranes beyond the 34th week of gestation (late PPROM) is frequently associated with the risk of the microbial invasion of the amniotic fluid (MIAC) and histological chorioamnionitis (HCA). Hence, we employed a Tandem Mass Tag-based approach to uncover amniotic fluid proteome response to the presence of MIAC and HCA in late PPROM. Protein dysregulation was associated with only five cases in the group of 15 women with confirmed MIAC and HCA. Altogether, 138 amniotic fluid proteins were changed in these five cases exclusively. These proteins were particularly associated with excessive neutrophil responses to infection, such as neutrophil degranulation and extracellular trap formation. We believe that the quantification of these proteins in amniotic fluid may assist in revealing women with the highest risk of excessive inflammatory response in late PPROM.Předčasná ruptura prebuněčných membrán po 34. týdnu těhotenství (pozdní PPROM) je často spojena s rizikem mikrobiální invaze plodové vody (MIAC) a histologické chorioamnionitidy (HCA). Proto jsme použili přístup založený na tandemové hmotnostní spektrometrii k odhalení reakce proteomu plodové vody na přítomnost MIAC a HCA v pozdní PPROM. Proteinová dysregulace byla spojena pouze s pěti případy ve skupině 15 žen s potvrzeným MIAC a HCA. Celkově bylo v těchto pěti případech změněno 138 proteinů plodové vody. Tyto proteiny byly zvláště spojeny s nadměrnými reakcemi neutrofilů na infekci, jako je degranulace neutrofilů a tvorba extracelulární pasti. Věříme, že kvantifikace těchto proteinů v plodové vodě může pomoci odhalit ženy s nejvyšším rizikem nadměrné zánětlivé odpovědi v pozdním PPROM