Insights on adaptive and innate immunity in canine leishmaniosis

Canine leishmaniosis (CanL) is caused by the parasite Leishmania infantum and is a systemic disease, which can present with variable clinical signs, and clinicopathological abnormalities. Clinical manifestations can range from subclinical infection to very severe systemic disease. Leishmaniosis is categorized as a neglected tropical disease and the complex immune responses associated with Leishmania species makes therapeutic treatments and vaccine development challenging for both dogs and humans. In this review, we summarize innate and adaptive immune responses associated with L. infantum infection in dogs, and we discuss the problems associated with the disease as well as potential solutions and the future direction of required research to help control the parasite

Multimodality Imaging Evaluation of a Singular Cardiac Structure

info:eu-repo/semantics/publishedVersio

Monitoramento da susceptibilidade de Euschistus heros (Hemiptera: Pentatomidae) aos inseticidas beta-ciflutrina e imidacloprido em Mato Grosso

Euschistus heros é um praga polifaga importante nos sistemas produtivos de grãos e fibras mato-grossenses, pois é a principal espécie de percevejo que ataca a cultura da soja, além de atacar o milho no início da emergência das plantas e as maças do algodoeiro. Portanto, sua permanência no campo é prolongada devido à extensa janela de cultivo no Estado. Consequentemente, o período de exposição aos inseticidas é potencializado quando comparado a sistemas produtivos com uma única safra que hospede a praga. Diante disso, objetivou-se monitorar os níveis de susceptibilidade do percevejo aos inseticidas imidacloprido e beta-ciflutrina. Para avaliar o nível de susceptibilidade das populações de campo, determinou-se uma dose diagnóstica em uma população susceptível de E. heros ao avaliar a mortalidade dos insetos em concentrações crescentes dos dois inseticidas. Desta forma, calculou-se a Concentração Letal para 95% dos indivíduos susceptíveis - CL95 , através da análise de probit. As doses diagnósticas estimadas foram de 3µl/ml (beta-ciflutrina/água) e 0,5µl/ml (imidacloprido/água). Os produtos comerciais utilizados continham as concentrações de 50 g/L de ingrediente ativo para beta-ciflutrina e 200 g/L de ingrediente ativo para imidacloprido. As populações de campo foram coletadas em soja na safra 2013/14 nos municípios de Rondonópolis, Nova Mutum e Sinop. Para a molécula imidacloprido, todas as populações tiveram uma mortalidade parecida com a população susceptível, porém para a molécula beta-ciflutrina a população de Rondonópolis diferenciou significativamente da população susceptível. Entretanto, falhas de controle em campo não são d iagnosticadas, pois os inseticidas registrados para o controle da praga que contém esses ingredientes ativos são uma mixtura de beta-ciflutrina com imidacloprido, assim promovendo efetivo controle devido ao imidacloprido

Effectiveness and safety of iodopovidone in an experimental pleurodesis model

OBJECTIVES: Chemical pleurodesis is an important therapeutic tool to control recurrent malignant pleural effusion. Among the various sclerosing agents, iodopovidone is considered effective and safe. However, in a recent study, ocular changes were described after iodopovidone was used in recurrent pneumothorax. The aim of the study was to evaluate the efficacy and morbidity of iodopovidone pleurodesis in an experimental model. METHODS: New Zealand rabbits were submitted to intrapleural injection of iodopovidone at concentrations of 2%, 4% and 10%. Biochemical (lactic dehydrogenase, proteins, triiodothyronine, free thyroxine, urea and creatinine) and immunological (Interleukin-8 [IL-8], VEGF and TGFβ) parameters were measured in the pleural fluid and blood. After 1, 3, 7, 14 and 28 days, groups of animals were euthanized, and macro- (pleura) and microscopic (pleura and retina) analyses were performed. RESULTS: An early pleural inflammatory response with low systemic repercussion was observed without corresponding changes in thyroid or renal function. The higher concentrations (4% and 10%) correlated with greater initial exudation, and maximum pleural thickening was observed after 28 days. No changes were observed in the retinal pigment epithelium of the rabbits. CONCLUSION: Iodopovidone is considered to be an effective and safe sclerosing agent in this animal model. However, its efficacy, tolerance and safety in humans should be further evaluated