Feeding behavior and forage intake of ewe lambs on pearl millet pasture with or without supplementation

Avaliaram-se o comportamento ingestivo e o consumo de matéria seca de cordeiras recebendo diferentes tipos de suplemento em pastagem de milheto (Pennisetum americanum (L.) Leeke). Foram utilizadas 24 cordeiras com peso inicial de 30,2 ± 2,6 kg, distribuídas em três estratégias alimentares: pastagem de milheto exclusivamente; pastagem de milheto e suplementação com farinha de mandioca; e pastagem de milheto e suplementação com glúten de milho. Os suplementos foram fornecidos na quantidade de 1% do PV. Foram avaliados, em três datas, os tempos diários (min/dia) de pastejo, ruminação e ócio e a taxa de bocados (boc/min) das cordeiras. O consumo de matéria seca (CMS) foi estimado pela relação entre a produção fecal e a indigestibilidade da matéria seca e a digestibilidade da MS, pelo método in vitro. A estimativa da excreção fecal foi realizada utilizando-se óxido de cromo como marcador externo. O delineamento experimental foi o inteiramente casualizado, com três estratégias alimentares, duas repetições de área e quatro animais-teste por repetição. O fornecimento de suplementos diminuiu o tempo de pastejo diário, a taxa de bocados e a massa de bocado. A ingestão de pasto foi menor entre as cordeiras que receberam glúten de milho que entre aquelas mantidas exclusivamente a pasto, todavia, a ingestão total de MS foi maior quando fornecidos os suplementos (947 g para suplementação com glúten de milho; 907 g para suplementação com farinha de mandioca; e 652 g para pastagem exclusiva). Cordeiras em pastagem de milheto sem suplementação permanecem mais tempo em pastejo.The feeding behavior and dry forage intake of ewe lambs receiving different types of supplementation on pearl millet (Pennisetum americanum (L.) Leeke) pasture was evaluated. Twenty-four ewe lambs with 30.2 ± 2.6 kg mean initial body weight (BW), distributed in three feeding strategies: grazing on pearl millet pasture; grazing on pearl millet pasture supplemented with cassava meal; and GL - grazing on pearl millet pasture supplemented with corn gluten meal. Supplements were provided in the amount of 1% of BW. The daily times (min/day) spent grazing, ruminating and idling, and the biting rate (bites/min) were evaluated on three dates. The dry matter intake (DMI) was estimated from the relationship between the fecal production (FC, g/day) and dry matter indigestibility by the in vitro method. The fecal excretion was estimated using chomium oxide as an outside marker. A randomized complete design was used, with three feeding strategies, two area replications and four tester animals per replication. Providing supplements decreased the daily grazing time, bite rate and bite mass. Pasture intake was lower among ewe lambs that received corn gluten than for those kept exclusively on pasture; however, dry matter total intake was higher when supplement fed (947 g for corn gluten supplementation; 907 g for cassava meal supplementation; and 652 g for exclusive pasture). Ewe lambs on pearl millet pasture without supplementation keep on grazing for more time

Carcass traits of culling ewes from Texel and Polwarth breeds submitted to two feeding systems

Avaliaram-se as características de carcaça de ovelhas de descarte das raças Ideal e Texel terminadas em dois sistemas de alimentação. Utilizaram-se 20 ovelhas (10 da raça Ideal e 10 da raça Texel) aleatoriamente distribuídas de acordo com o grupo genético em dois manejos alimentares (confinamento e pastagem cultivada). Os abates foram realizados quando os animais atingiram 3,5 pontos de condição corporal. Os pesos de carcaça quente (PCQ) e de carcaça fria (PCF) e os rendimentos de carcaça quente (RCQ) e de carcaça fria (RCF) das ovelhas Texel foram maiores que os das ovelhas Ideal: 27,85 e 19,04 kg; 27,08 e 18,43 kg; 47,25 e 45,20% e 45,95 e 43,72%, respectivamente. O peso dos cortes perna, paleta, pescoço e costela e os pesos de músculos, ossos e gordura da perna dos animais da raça Texel foram superiores aos obtidos na raça Ideal. Quando avaliadas em porcentagem do peso corporal, no entanto, essas características não diferiram entre as duas raças. O manejo alimentar não influenciou as características de carcaça de ovelhas de descarte. Ovelhas Texel apresentam cortes maiores que os de ovelhas Ideal.Carcass traits of culling ewes from Texel and Polwarth breeds submitted to two feeding systems were evaluated. Twenty ewes (10 from Polwarth breed and 10 from Texel breed) were randomly distributed according to its genetic group, in two feeding systems (feedlot and cultivated pasture). The slaughter was carried out when the animals reached body condition score of 3.5 points. Hot carcass (HCW) and cold carcass (CCW) weights and hot carcass (HCD) and cold carcass (CCD) dressing of Texel ewes were higher than those of Polwarth ewes: 27.85 and 19.04 kg, 27.08 and 18.43 kg, 47.25 and 45.20 and 45.95 and 43.72%, respectively. The absolute weights of cuts for leg, shoulder, neck and rib, and absolute weights for muscles, bones and leg fat of ewes from Texel breed were higher than those obtained by Polwarth breed. When evaluated as relative weights, however, these characteristics did not differ between the two breeds. The feeding system did not constitute a change factor for carcass traits of culling ewes. Texel ewes breed showed cuts with higher weights than those obtained by Polwarth ewes breed

Alteração da carga de endoparasitas em ovinos submetidos a diferentes níveis de folha de bananeira na alimentação.

Vinte e seis borregas (Texel x Corriedale), com idade entre 4 e 5 meses, foram utilizados para avaliar o efeito da administração de lâminas foliares de bananeira (Musa spp.) na alimentação sobre o controle de nematódeos gastrintestinais, através da contagem de ovos/grama de fezes (OPG). Os tratamentos foram constituídos pelo controle negativo, e pelos grupos de borregas que consumiram lâminas foliares frescas de bananeira entre 0,40 e 0,80% e de 0,81 a 1,20% do peso vivo, por três dias consecutivos. Após 15 dias, esse processo foi repetido. Antes e após o fornecimento da bananeira às borregas (no 1o, 3o, 5o, 7o, 9o, 11o e 14o dias), foram coletadas amostras de fezes. Os valores médios de redução de OPG foram de 3,5; 27,6 e 46,7%, respectivamente. Resultado superior (P<0,05) foi detectado no grupo de ovinos que apresentaram maior consumo

A list of land plants of Parque Nacional do Caparaó, Brazil, highlights the presence of sampling gaps within this protected area

Brazilian protected areas are essential for plant conservation in the Atlantic Forest domain, one of the 36 global biodiversity hotspots. A major challenge for improving conservation actions is to know the plant richness, protected by these areas. Online databases offer an accessible way to build plant species lists and to provide relevant information about biodiversity. A list of land plants of “Parque Nacional do Caparaó” (PNC) was previously built using online databases and published on the website "Catálogo de Plantas das Unidades de Conservação do Brasil." Here, we provide and discuss additional information about plant species richness, endemism and conservation in the PNC that could not be included in the List. We documented 1,791 species of land plants as occurring in PNC, of which 63 are cited as threatened (CR, EN or VU) by the Brazilian National Red List, seven as data deficient (DD) and five as priorities for conservation. Fifity-one species were possible new ocurrences for ES and MG states

Molecular and cellular biomarkers of COVID-19 prognosis : protocol for the prospective cohort TARGET study

Background: Since the beginning of the COVID-19 pandemic, the world’s attention has been focused on better understanding the relation between the human host and the SARS-CoV-2 virus, as its action has led to hundreds of thousands of deaths. Objective: In this context, we decided to study certain consequences of the abundant cytokine release over the innate and adaptive immune systems, inflammation, and hemostasis, comparing mild and severe forms of COVID-19. Methods: To accomplish these aims, we will analyze demographic characteristics, biochemical tests, immune biomarkers, leukocyte phenotyping, immunoglobulin profile, hormonal release (cortisol and prolactin), gene expression, thromboelastometry, neutralizing antibodies, metabolic profile, and neutrophil function (reactive oxygen species production, neutrophil extracellular trap production, phagocytosis, migration, gene expression, and proteomics). A total of 200 reverse transcription polymerase chain reaction–confirmed patients will be enrolled and divided into two groups: mild/moderate or severe/critical forms of COVID-19. Blood samples will be collected at different times: at inclusion and after 9 and 18 days, with an additional 3-day sample for severe patients. We believe that this information will provide more knowledge for future studies that will provide more robust and useful clinical information that may allow for better decisions at the front lines of health care. Results: The recruitment began in June 2020 and is still in progress. It is expected to continue until February 2021. Data analysis is scheduled to start after all data have been collected. The coagulation study branch is complete and is already in the analysis phase

Panoramic snapshot of serum soluble mediator interplay in pregnant women with convalescent COVID-19: an exploratory study

IntroductionSARS-CoV-2 infection during pregnancy can induce changes in the maternal immune response, with effects on pregnancy outcome and offspring. This is a cross-sectional observational study designed to characterize the immunological status of pregnant women with convalescent COVID-19 at distinct pregnancy trimesters. The study focused on providing a clear snapshot of the interplay among serum soluble mediators.MethodsA sample of 141 pregnant women from all prenatal periods (1st, 2nd and 3rd trimesters) comprised patients with convalescent SARS-CoV-2 infection at 3-20 weeks after symptoms onset (COVID, n=89) and a control group of pre-pandemic non-infected pregnant women (HC, n=52). Chemokine, pro-inflammatory/regulatory cytokine and growth factor levels were quantified by a high-throughput microbeads array.ResultsIn the HC group, most serum soluble mediators progressively decreased towards the 2nd and 3rd trimesters of pregnancy, while higher chemokine, cytokine and growth factor levels were observed in the COVID patient group. Serum soluble mediator signatures and heatmap analysis pointed out that the major increase observed in the COVID group related to pro-inflammatory cytokines (IL-6, TNF-α, IL-12, IFN-γ and IL-17). A larger set of biomarkers displayed an increased COVID/HC ratio towards the 2nd (3x increase) and the 3rd (3x to 15x increase) trimesters. Integrative network analysis demonstrated that HC pregnancy evolves with decreasing connectivity between pairs of serum soluble mediators towards the 3rd trimester. Although the COVID group exhibited a similar profile, the number of connections was remarkably lower throughout the pregnancy. Meanwhile, IL-1Ra, IL-10 and GM-CSF presented a preserved number of correlations (≥5 strong correlations in HC and COVID), IL-17, FGF-basic and VEGF lost connectivity throughout the pregnancy. IL-6 and CXCL8 were included in a set of acquired attributes, named COVID-selective (≥5 strong correlations in COVID and &lt;5 in HC) observed at the 3rd pregnancy trimester.Discussion and conclusionFrom an overall perspective, a pronounced increase in serum levels of soluble mediators with decreased network interplay between them demonstrated an imbalanced immune response in convalescent COVID-19 infection during pregnancy that may contribute to the management of, or indeed recovery from, late complications in the post-symptomatic phase of the SARS-CoV-2 infection in pregnant women