Domestic science club demonstration

Citation: Pierce, Marcia. Domestic science club demonstration. Senior thesis, Kansas State Agricultural College, 1908.Introduction: Food has been described as those substances which taken into the body may be used to build tissue, repair waste and furnish any form of energy. In preparing foods in this short course of Domestic Science, it shall be the object to prepare them in the best possible way. The senses of taste and sight should always be appealed to

Characterization of the adherence mechanisms of Listeria monocytogenes to host cells and their role in the pathogenesis of listeriosis

Listeria monocytogenes is the facultative intracellular pathogen responsible for causing the foodborne illness known as listeriosis. Disease occurs following invasion of cells of the intestinal tract by the organism following consumption of contaminated foods. In this study, a mechanism of opsonin-independent attachment of L. monocytogenes to host cells was identified. A clinical isolate of L. monocytogenes, together with murine-derived primary peritoneal macrophages were used to study this adherence phenomenon. Transmission and scanning electron microscopy were used to visualize the attachment and uptake of L. monocytogenes by murine macrophages, while organism enumeration by viable bacterial cell colony counts was used as a measure of intracellular replication in host cells. L. monocytogenes was shown to adhere to host cells in the absence of complement and a complete infectious cycle for the organism was found. These data confirmed previously identified stages of host cell infection by L. monocytogenes in other cell types, including adherence, uptake, phagosomal escape, and intracellular replication. The nature of the L. monocytogenes bacterial adhesin and the host cell receptor in binding studies was investigated. The results from competitive binding studies indicated the involvement of N-acetyl neuraminic acid, a member of the sialic acid group, in the binding of L. monocytogenes to murine peritoneal macrophages. Identification of the host cell receptor was not possible, as most treatments were ineffective at preventing organism binding. Monoclonal antibodies directed against complement receptor 3 were used in blocking studies and these indicated that CR3 is not involved in the recognition of L. monocytogenes by nonlistericidal, thioglycollate-elicited macrophages. These results indicated the importance of opsonin-independent binding mechanisms for L. monocytogenes and shed new light on our understanding of the infectious processes of this pathogen

Technology Learning Impact on Pre-service Teacher Education Candidates After Implementation of a Web-Based E-portfolio

This study examined the role of student competence, attitude, and training on the production of student e-portfolios. Neither student background nor experience had a significant impact on their attitude toward developing an e-portfoli

From Zero to Over 2,500 Eportfolios in Six Years: The Eastern Kentucky University Experience

In 2000, Eastern Kentucky University (EKU) was awarded a Preparing Tomorrows Teachers to Use Technology (PT3) Implementation grant. One of the major goals of the grant was to create an electronic/multimedia portfolio (eportfolio) assessment system through which future teachers would document their proficiencies and amass strategies to enhance their future teaching. Between the fall of 2000 and the summer of 2003 an eportfolio development team, consisting of faculty from the College of Education and the College of Arts and Sciences, a college student, a public school teacher, and a technology expert, developed and implemented an eportfolio to be used by all teacher education candidates in the College of Education. Through systematic piloting and review, the obstacles and challenges of developing an eportfolio were met and a professional product was incorporated into the teacher education program in the College of Education. As of spring 2006, over 2,500 College of Education student eportfolios are online

Immunohistochemical Analysis of IL-1 beta in the Discs of Patients with Temporomandibular Joint Dysfunction

Purpose: Interleukin-1 beta (IL-1β) is a cytokine that participates in the regulation of immune responses and inflammatory reactions. It is hypothesized that IL-1 levels may be elevated in patients suffering from temporomandibular joint dysfunction. The purpose of this study was to determine the association of IL-1β expression with TMD using an immunohistochemical approach to evaluate the joint disc. Materials and methods: A total of 39 human temporomandibular joint disc samples were collected, with 31 samples in the test group. Nineteen of the test group samples were from discs of patients with anterior disc displacement with reduction, and 12 of the samples were from patients with anterior disc displacement without reduction. Eight control samples were used in the control group. The samples were immunostained and evaluated on both quantity and intensity of staining. Results: There was a statistically significant difference (p \u3c 0.05) between the control and test groups for both quantity and intensity of staining. Conclusion: IL-1β plays a role in the inflammatory process and degradation of TMJ discs in patients with TMJ dysfunctions

Racial and Ethnic Diversity in Academic Emergency Medicine: How Far Have We Come? Next Steps for the Future

Although the U.S. population continues to become more diverse, black, Hispanic, and Native American doctors remain underrepresented in emergency medicine (EM). The benefits of a diverse medical workforce have been well described, but the percentage of EM residents from underrepresented groups is small and has not significantly increased over the past 20 years. A group of experts in the field of diversity and inclusion convened a work group during the Council of Emergency Medicine Residency Program Directors (CORD) and Society for Academic Emergency Medicine (SAEM) national meetings. The objective of the discussion was to develop strategies to help EM residency programs examine and improve racial and ethnic diversity in their institutions. Specific recommendations included strategies to recruit racially and ethnically diverse residency candidates and strategies to mentor, develop, retain, and promote minority faculty.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/147225/1/aet210204.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/147225/2/aet210204_am.pd

Physical activity, additional breast cancer events, and mortality among early-stage breast cancer survivors: findings from the WHEL Study

ObjectiveResearch suggests that physical activity is associated with improved breast cancer survival, yet no studies have examined the association between post-diagnosis changes in physical activity and breast cancer outcomes. The aim of this study was to determine whether baseline activity and 1-year change in activity are associated with breast cancer events or mortality.MethodsA total of 2,361 post-treatment breast cancer survivors (Stage I-III) enrolled in a randomized controlled trial of dietary change completed physical activity measures at baseline and one year. Physical activity variables (total, moderate-vigorous, and adherence to guidelines) were calculated for each time point. Median follow-up was 7.1 years. Outcomes were invasive breast cancer events and all-cause mortality.ResultsThose who were most active at baseline had a 53% lower mortality risk compared to the least active women (HR = 0.47; 95% CI: 0.26, 0.84; p = .01). Adherence to activity guidelines was associated with a 35% lower mortality risk (HR = 0.65, 95% CI: 0.47, 0.91; p < .01). Neither baseline nor 1-year change in activity was associated with additional breast cancer events.ConclusionsHigher baseline (post-treatment) physical activity was associated with improved survival. However, change in activity over the following year was not associated with outcomes. These data suggest that long-term physical activity levels are important for breast cancer prognosis

Long-term efficacy and safety of fostemsavir among subgroups of heavily treatment-experienced adults with HIV-1

Objectives: The aim of this study was to understand how demographic and treatment-related factors impact responses to fostemsavir-based regimens. Design: BRIGHTE is an ongoing phase 3 study evaluating twice-daily fostemsavir 600 mg and optimized background therapy (OBT) in heavily treatment-experienced individuals failing antiretroviral therapy with limited treatment options (Randomized Cohort 1-2 and Nonrandomized Cohort 0 fully active antiretroviral classes). Methods: Virologic response rates (HIV-1 RNA <40 copies/ml, Snapshot analysis) and CD4+ T-cell count increases in the Randomized Cohort were analysed by prespecified baseline characteristics (age, race, sex, region, HIV-1 RNA, CD4+ T-cell count) and viral susceptibility to OBT. Safety results were analysed by baseline characteristics for combined cohorts (post hoc). Results: In the Randomized Cohort, virologic response rates increased between Weeks 24 and 96 across most subgroups. Virologic response rates over time were most clearly associated with overall susceptibility scores for new OBT agents (OSS-new). CD4+ T-cell count increases were comparable across subgroups. Participants with baseline CD4+ T-cell counts less than 20 cells/μl had a mean increase of 240 cells/μl. In the safety population, more participants with baseline CD4+ T-cell counts less than 20 vs. at least 200 cells/μl had grade 3/4 adverse events [53/107 (50%) vs. 24/96 (25%)], serious adverse events [58/107 (54%) vs. 25/96 (26%)] and deaths [16/107 (15%) vs. 2/96 (2%)]. There were no safety differences by other subgroups. Conclusion: Week 96 results for BRIGHTE demonstrate comparable rates of virologic and immunologic response (Randomized Cohort) and safety (combined cohorts) across subgroups. OSS-new is an important consideration when constructing optimized antiretroviral regimens for heavily treatment-experienced individuals with limited remaining treatment options

Loss-of-function mutations in UDP-Glucose 6-Dehydrogenase cause recessive developmental epileptic encephalopathy

AbstractDevelopmental epileptic encephalopathies are devastating disorders characterized by intractable epileptic seizures and developmental delay. Here, we report an allelic series of germline recessive mutations in UGDH in 36 cases from 25 families presenting with epileptic encephalopathy with developmental delay and hypotonia. UGDH encodes an oxidoreductase that converts UDP-glucose to UDP-glucuronic acid, a key component of specific proteoglycans and glycolipids. Consistent with being loss-of-function alleles, we show using patients’ primary fibroblasts and biochemical assays, that these mutations either impair UGDH stability, oligomerization, or enzymatic activity. In vitro, patient-derived cerebral organoids are smaller with a reduced number of proliferating neuronal progenitors while mutant ugdh zebrafish do not phenocopy the human disease. Our study defines UGDH as a key player for the production of extracellular matrix components that are essential for human brain development. Based on the incidence of variants observed, UGDH mutations are likely to be a frequent cause of recessive epileptic encephalopathy.</jats:p