Indicadores bibliométricos de actividad de la revista MVZ Córdoba 1994-2008

Objetivo. Analizar el comportamiento bibliométrico de la revista MVZ-Córdoba y visualizar indicadores que permitan la toma de decisiones enfocadas a mejorar la calidad de la publicación. Materiales y métodos. Se emplearon los volúmenes del 1 al 13 compuestos por 49.2% de artículos originales, 17.4% de resúmenes de trabajos de grado, 17.7% de otros documentos, 8.9% de artículos de revisión, 4.3% de casos clínicos, 1.3% de artículos de opinión, 1.0% de comunicaciones breves, 0.3% de artículos institucionales, para un total de 305 manuscritos, 915 autores y 4881 referencias. Se determinaron los índices de colaboración (IC), productividad (IP), Price (IO), obsolescencia, aislamiento (IA), autocitación (SCR); también se evaluó la distribución porcentual y tipo de artículos, el número de autores y el promedio de citas. Resultados. Los índices generales IC, IP, IO, IA y SCR fueron 3.0, 2.18, 30.3, 7.67 y 8.9 % respectivamente; el análisis de Burton-Kebler reportó un envejecimiento anual que osciló entre 90.5 y 95.1%, lo que significa una pérdida de actualidad entre 4.93 y 9.52%. Conclusiones. La revista ha mostrado una calidad ascendente lo que se corrobora con la indización internacional. Para mantener y aumentar la calidad de la revista se debe reglamentar y verificar la actualidad de las referencias, controlar el SCR, el IA, aumentar el índice de Price, la vida media, realizar mediciones bibliométricas con mayor periodicidad, así como establecer la vigilancia sobre los índices bibliométricos que se generen de las indizaciones de la revista MVZ Córdoba

COMPARACIÓN CINÉTICA Y BIOQUÍMICA DE TRES CEPAS DE Clostridium tetani, PARA LA PRODUCCIÓN DE TOXINA TETÁNICA

In this study was compared the biochemical and kinetic behavior of three strains of Clostridium tetani:Harvard (Ha), Massachusetts (Ms) and Caracas (Ca), strains commonly used for the production of thetetanic toxoid. Biochemical tests for anaerobic microorganisms identification were done and cultures in5L reactor were developed, under the following conditions: Latham means Mueller “With” and “Without”sodium glutamate; 1% (v/v) of inoculum, initial pH 7.1 ± 0.2 (variable), 35ºC and 100 r.p.m. The resultsshowed similar biochemical characteristics between the strains including the ATCC 9441 control. Thekinetic behavior of the Ms and Ha strains was similar for all the evaluated parameters, obtaining greaterproductivity in cultures supplemented with 2.5g/L of sodium glutamate (Ms: Without: 625Lf/L.h andWith: 658Lf/L.h; Ha: Without: 610Lf/L.h and With: 658Lf/L.h). The Ca strain, produced greater amount ofbiomass (Ca: 0.5420g/L; Ms: 0.2721g/L and Ha: 0.2009g/L), nevertheless, its toxin productivity wassmaller (Without: 174Lf/L.h and With: 417Lf/L.h). These results discard the use of the Ca strain for theproduction of the toxoid, under the assayed fermentation conditions

Prevalence of Listeria monocytogenes in pork-meat and other processed products from the Colombian swine industry

ABSTRACT Objective. To determine the prevalence of L. monocytogenes in pork carcasses, meat cuts, and meat products (“chorizo”, sausage and ham). Materials and methods. Stratified sampling was implemented in meat-processed products. We analyzed 566 (37%) carcasses, 472 (31%) meat cuts, and 481, (32%) meat-processed products, distributed as follows: 169 (11%) sausage, 163 (11%) ham, and 149 (10%) “chorizo”, for a total of 1519 (100%) samples in a period of 18 months. The samples were processed using the ISO-17604, ISO-11290-1 and the USDA/FSIS (MLG-8.03) methods. Genus and species were confirmed by multiplex-PCR. Results. We obtained isolates of L. monocytogenes from 21 carcasses (10%), 160 (76%) from meat deboning, 10 (5%) from ham, 6 (3%) from “chorizo”, and 13 (6%) from sausage. The prevalence found was 3.7% and 33.9% in carcasses and meat deboning respectively. The prevalence in the meat-processed products was 4.03% in “chorizo”, 6.13% in ham and 7.69% in sausage. The overall prevalence of L. monocytogenes in the study was 13.82%. Conclusions. We found L. monocytogenes in different products analyzed, with particular interest in ham and sausage since both are consumed without previous heat treatmen

Prevalencia de Salmonella spp., en ganglios mesentéricos de porcinos en plantas de beneficio Colombianas

Objective. Objective was to determine the prevalence of Salmonella spp., in pigs mesenteric ganglion, from different regions of Colombia. Materials and Methods. A stratified sampling by proportional fixation was carried out at benefit plants of each of the 13 participating departments, whose pork production volume is representative at national level. Sampling was performed during five months, for a total of 457 samples analyzed. Salmonella spp., identification was performed by the MDS Molecular System, later isolates were confirmed in Maldi-TOF MS. Antimicrobial susceptibility of the isolates was determined using the B1016-180 panel and statistical analysis was performed in Whonet 2016, some of the multi-resistant isolates were them serotyped by Kauffman-White method. Results. National prevalence was 28.2%, with the presence of S. typhimurium, S. Agama, S. London, S. Agona, S. Haifa and S. 1,4,12: i: -. Resistance to antibiotics frequently used in human (23.6% Trimethoprim/Sulfamethoxazole, 2.7% Cefotaxime (CTX), 11.8% Ampicillin (AMP) and 1.8% Ciprofloxacin) was found. Conclusion. The prevalence of Salmonella in mesenteric ganglia was 28.2%, being the Huila region the one with the highest prevalence, recovering atypical serotypes such as S. London and S. Haifa.Objetivo. El objetivo fue determinar la prevalencia de Salmonella spp., en ganglios mesentéricos de porcinos, provenientes de diferentes regiones de Colombia. Materiales y Métodos. Se realizó un muestreo estratificado por fijación proporcional en plantas de beneficio, de cada uno de los 13 departamentos participantes, cuyo volumen de producción de carne de cerdo es representativo a nivel nacional. El muestreo se realizó durante cinco meses, para un total de 457 muestras analizadas. La identificación de Salmonella spp., se realizó mediante el Sistema Molecular MDS, luego los aislamientos fueron confirmados por Maldi-TOF MS. Se determinó la susceptibilidad antimicrobiana de los aislamientos usando el panel B1016-180 y el análisis estadístico se realizó en Whonet 2016, posteriormente algunos de los aisalmientos multi-resistentes fueron serotipificados por el método de Kauffman-White. Resultados. La prevalencia nacional fue 28.2%, con presencia de los serotipos S. Typhimurium, S. Agama, S. London, S. Agona, S. Haifa y S. 1,4,12: i : --. Se encontró resistencia a antibióticos de uso frecuente en humanos (23.6% Trimetoprim/Sulfametoxazol, 2.7% Cefotaxime (CTX), 11.8% Ampicilina (AMP) y 1.8% Ciprofloxacina). Conclusión. La prevalencia de Salmonella en ganglios mesénticos fue del 28.2%, siendo la región del Huila la que más aportó, se recuperaron serotipos atípicos como S. London y S. Haif

Low-Scale Expression and Purification of an Active Putative Iduronate 2-Sulfate Sulfatase-Like Enzyme from Escherichia coli K12

The sulfatase family involves a group of enzymes with a large degree of similarity. Until now, sixteen human sulfatases have been identified, most of them found in lysosomes. Human deficiency of sulfatases generates various genetic disorders characterized by abnormal accumulation of sulfated intermediate compounds. Mucopolysaccharidosis type II is characterized by the deficiency of iduronate 2-sulfate sulfatase (IDS), causing the lysosomal accumulation of heparan and dermatan sulfates. Currently, there are several cases of genetic diseases treated with enzyme replacement therapy, which have generated a great interest in the development of systems for recombinant protein expression. In this work we expressed the human recombinant IDS-Like enzyme (hrIDS-Like) in Escherichia coli DH5α. The enzyme concentration revealed by ELISA varied from 78. 13 to 94. 35 ng/ml and the specific activity varied from 34. 20 to 25. 97 nmol/h/mg. Western blotting done after affinity chromatography purification showed a single band of approximately 40 kDa, which was recognized by an IgY polyclonal antibody that was developed against the specific peptide of the native protein. Our 100 ml-shake-flask assays allowed us to improve the enzyme activity seven fold, compared to the E. coli JM109/pUC13-hrIDS-Like system. Additionally, the results obtained in the present study were equal to those obtained with the Pichia pastoris GS1115/pPIC-9-hrIDS-Like system (3 L bioreactor scale). The system used in this work (E. coli DH5α/pGEX-3X-hrIDS-Like) emerges as a strategy for improving protein expression and purification, aimed at recombinant protein chemical characterization, future laboratory assays for enzyme replacement therapy, and as new evidence of active putative sulfatase production in E. coli. © 2013 The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg.Fil: Morales Álvarez, Edwin David. Universidad del Quindio; ColombiaFil: Rivera Hoyos, Claudia Marcela. Universidad del Quindio. Facultad de Medicina; ColombiaFil: Baena Moncada, Angelica Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Landázuri, Patricia. Universidad del Quindio. Facultad de Medicina. Centro de Investig. Biomédicas; ColombiaFil: Poutou Piñales, Raúl A.. Pontificia Universidad Javeriana; ColombiaFil: Sáenz Suárez, Homero. Universidad del Quindio; ColombiaFil: Barrera, Luis A.. Pontificia Universidad Javeriana; ColombiaFil: Echeverri Peña, Olga Y.. Pontificia Universidad Javeriana; Colombi

Production of recombinant trichoderma reesei cellobiohydrolase ii in a new expression system based on wickerhamomyces anomalus

Q31-7Cellulase is a family of at least three groups of enzymes that participate in the sequential hydrolysis of cellulose. Recombinant expression of cellulases might allow reducing their production times and increasing the low proteins concentrations obtained with filamentous fungi. In this study, we describe the production of Trichoderma reesei cellobiohydrolase II (CBHII) in a native strain of Wickerhamomyces anomalus. Recombinant CBHII was expressed in W. anomalus 54-A reaching enzyme activity values of up to 14.5 U L−1. The enzyme extract showed optimum pH and temperature of 5.0–6.0 and 40°C, respectively. Enzyme kinetic parameters ( of 2.73 mM and Vmax of 23.1 µM min−1) were between the ranges of values reported for other CBHII enzymes. Finally, the results showed that an enzymatic extract of W. anomalus 54-A carrying the recombinant T. reesei CBHII allows production of reducing sugars similar to that of a crude extract from cellulolytic fungi. These results show the first report on the use of W. anomalus as a host to produce recombinant proteins. In addition, recombinant T. reesei CBHII enzyme could potentially be used in the degradation of lignocellulosic residues to produce bioethanol, based on its pH and temperature activity profile