1,486 research outputs found
Replication stress by Py–Im polyamides induces a non-canonical ATR-dependent checkpoint response
Pyrrole–imidazole polyamides targeted to the androgen response element were cytotoxic in multiple cell lines, independent of intact androgen receptor signaling. Polyamide treatment induced accumulation of S-phase cells and of PCNA replication/repair foci. Activation of a cell cycle checkpoint response was evidenced by autophosphorylation of ATR, the S-phase checkpoint kinase, and by recruitment of ATR and the ATR activators RPA, 9-1-1, and Rad17 to chromatin. Surprisingly, ATR activation was accompanied by only a slight increase in single-stranded DNA, and the ATR targets RPA2 and Chk1, a cell cycle checkpoint kinase, were not phosphorylated. However, ATR activation resulted in phosphorylation of the replicative helicase subunit MCM2, an ATR effector. Polyamide treatment also induced accumulation of monoubiquitinated FANCD2, which is recruited to stalled replication forks and interacts transiently with phospho-MCM2. This suggests that polyamides induce replication stress that ATR can counteract independently of Chk1 and that the FA/BRCA pathway may also be involved in the response to polyamides. In biochemical assays, polyamides inhibit DNA helicases, providing a plausible mechanism for S-phase inhibition
Structure and relaxations in liquid and amorphous Selenium
We report a molecular dynamics simulation of selenium, described by a
three-body interaction. The temperatures T_g and T_c and the structural
properties are in agreement with experiment. The mean nearest neighbor
coordination number is 2.1. A small pre-peak at about 1 AA^-1 can be explained
in terms of void correlations. In the intermediate self-scattering function,
i.e. the density fluctuation correlation, classical behavior, alpha- and
beta-regimes, is found. We also observe the plateau in the beta-regime below
T_g. In a second step, we investigated the heterogeneous and/or homogeneous
behavior of the relaxations. At both short and long times the relaxations are
homogeneous (or weakly heterogeneous). In the intermediate time scale, lowering
the temperature increases the heterogeneity. We connect these different domains
to the vibrational (ballistic), beta- and alpha-regimes. We have also shown
that the increase in heterogeneity can be understood in terms of relaxations
How the Kano model contributes to Kansei engineering in services
Recent studies show that products and services hold great appeal if they are attractively designed to elicit emotional feelings from customers. Kansei engineering (KE) has good potential to provide a competitive advantage to those able to read and translate customer affect and emotion in actual product and services. This study introduces an integrative framework of the Kano model and KE, applied to services. The Kano model was used and inserted into KE to exhibit the relationship between service attribute performance and customer emotional response. Essentially, the Kano model categorises service attribute quality into three major groups (must-be [M], one-dimensional [O] and attractive [A]). The findings of a case study that involved 100 tourists who stayed in luxury 4- and 5-star hotels are presented. As a practical matter, this research provides insight on which service attributes deserve more attention with regard to their significant impact on customer emotional needs.
Statement of Relevance: Apart from cognitive evaluation, emotions and hedonism play a big role in service encounters. Through a focus on delighting qualities of service attributes, this research enables service providers and managers to establish the extent to which they prioritise their improvement efforts and to always satisfy their customer emotions beyond expectation.
Keywords:
Kansei engineering, emotional feelings, Kano model, service
On the Evolution of and High-Energy Emission from GHz-Peaked-Spectrum Sources
Here we discuss evolution and broad-band emission of compact (< kpc) lobes in
young radio sources. We propose a simple dynamical description for these
objects, consisting of a relativistic jet propagating into a uniform gaseous
medium in the central parts of an elliptical host. In the framework of the
proposed model, we follow the evolution of ultrarelativistic electrons injected
from a terminal hotspot of a jet to expanding lobes, taking into account their
adiabatic energy losses as well as radiative cooling. This allows us to discuss
the broad-band lobe emission of young radio sources. In particular, we argue
that the observed spectral turnover in the radio synchrotron spectra of these
objects cannot originate from the synchrotron self-absorption process but is
most likely due to free-free absorption effects connected with neutral clouds
of interstellar medium engulfed by the expanding lobes and photoionized by
active centers. We also find a relatively strong and complex high-energy
emission component produced by inverse-Compton up-scattering of various
surrounding photon fields by the lobes' electrons. We argue that such high
energy radiation is strong enough to account for several observed properties of
GHz-peaked-spectrum (GPS) radio galaxies at UV and X-ray frequencies. In
addition, this emission is expected to extend up to GeV (or possibly even TeV)
photon energies and can thus be probed by several modern gamma-ray instruments.
In particular, we suggest that GPS radio galaxies should constitute a
relatively numerous class of extragalactic sources detected by GLAST.Comment: 32 pages, 3 figures included. Revised version, accepted for
publication in Ap
Phase diagram of the metal-insulator transition in 2D electronic systems
We investigated the interdependence of the effects of disorder and carrier
correlations on the metal-insulator transition in two-dimensional electronic
systems. We present a quantitative metal-insulator phase diagram. Depending on
the carrier density we find two different types of metal-insulator transition -
a continuous localization for rs=<8 and a discontinuous transition at higher
rs. The critical level of disorder at the transition decreases with decreasing
carrier density. At very low carrier densities we find that the system is
always insulating. The value of the conductivity at the transition is
consistent with recent experimental measurements. The self-consistent method
which we have developed includes the effects of both disorder and correlations
on the transition, using a density relaxation theory with the Coulomb
correlations determined from numerical simulation data.Comment: 4 pages, RevTeX + epsf, 5 figures. New comments on conducting phase
and on the conductivity. References updated and correcte
MicroRNA-221 and -222 modulate intestinal inflammatory Th17 cell response as negative feedback regulators downstream of interleukin-23
Mikami et al. examine the role of miR-221/222 in helper T cells in the gut. MiR-221/222 are induced by IL-23 and suppressed by TGFβ, targeting Maf and IL23r for degradation. During inflammation, these miRNAs serve as a negative feedback rheostat to constrain IL23-Th17 cell responses
Muon capture by 3He nuclei followed by proton and deuteron production
The paper describes an experiment aimed at studying muon capture by
nuclei in pure and mixtures at various densities. Energy distributions of
protons and deuterons produced via and are measured for the
energy intervals MeV and MeV, respectively. Muon capture
rates, and are obtained using two different analysis methods. The
least--squares methods gives , . The Bayes theorem
gives ,
. The experimental
differential capture rates, and , are compared with theoretical
calculations performed using the plane--wave impulse approximation (PWIA) with
the realistic NN interaction Bonn B potential. Extrapolation to the full energy
range yields total proton and deuteron capture rates in good agreement with
former results.Comment: 17 pages, 13 figures, accepted for publication in PR
A New 5-Flavour LO Analysis and Parametrization of Parton Distributions in the Real Photon
New, radiatively generated, LO quark (u,d,s,c,b) and gluon densities in a
real, unpolarized photon are presented. We perform a global 3-parameter fit,
based on LO DGLAP evolution equations, to all available data for the structure
function F2^gamma(x,Q^2). We adopt a new theoretical approach called ACOT(chi),
originally introduced for the proton, to deal with the heavy-quark thresholds.
This defines our basic model (CJKL model), which gives a very good description
of the experimental data on F2^gamma(x,Q^2), for both Q^2 and x dependences.
For comparison we perform a standard fit using the Fixed Flavour-Number Scheme
(FFNS_CJKL model), updated with respect to the previous fits of this type. We
show the superiority of the CJKL fit over the FFNS_CJKL one and other LO fits
to the F2^gamma(x,Q^2) data. The CJKL model gives also the best description of
the LEP data on the Q^2 dependence of the F2^gamma, averaged over various
x-regions, and the F_2,c^gamma, which were not used directly in the fit.
Finally, a simple analytic parametrization of the resulting parton densities
obtained with the CJKL model is given.Comment: 43 pages, RevTeX4 using axodraw style, 3 tex and 12 postscript
figures, version submitted to Phys. Rev. D, small text changes, one reference
added, FORTRAN program available at http://www.fuw.edu.pl/~pjank/param.html
and at http://www-zeuthen.desy.de/~alorca/id4.htm
Photoproduction of phi(1020) mesons on the proton at large momentum transfer
The cross section for meson photoproduction on the proton has been
measured for the first time up to a four-momentum transfer -t = 4 GeV^2, using
the CLAS detector at the Thomas Jefferson National Accelerator Facility. At low
four-momentum transfer, the differential cross section is well described by
Pomeron exchange. At large four-momentum transfer, above -t = 1.8 GeV^2, the
data support a model where the Pomeron is resolved into its simplest component,
two gluons, which may couple to any quark in the proton and in the .Comment: 5 pages; 7 figure
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