Critical role of canonical transient receptor potential channel 7 in initiation of seizures

Status epilepticus (SE) is a life-threatening disease that has been recognized since antiquity but still causes over 50,000 deaths annually in the United States. The prevailing view on the pathophysiology of SE is that it is sustained by a loss of normal inhibitory mechanisms of neuronal activity. However, the early process leading to the initiation of SE is not well understood. Here, we show that, as seen in electroencephalograms, SE induced by the muscarinic agonist pilocarpine in mice is preceded by a specific increase in the gamma wave, and genetic ablation of canonical transient receptor potential channel (TRPC) 7 significantly reduces this pilocarpine-induced increase of gamma wave activity, preventing the occurrence of SE. At the cellular level, TRPC7 plays a critical role in the generation of spontaneous epileptiform burst firing in cornu ammonis (CA) 3 pyramidal neurons in brain slices. At the synaptic level, TRPC7 plays a significant role in the long-term potentiation at the CA3 recurrent collateral synapses and Schaffer collateral-CA1 synapses, but not at the mossy fiber-CA3 synapses. Taken together, our data suggest that epileptiform burst firing generated in the CA3 region by activity-dependent enhancement of recurrent collateral synapses may be an early event in the initiation process of SE and that TRPC7 plays a critical role in this cellular event. Our findings reveal that TRPC7 is intimately involved in the initiation of seizures both in vitro and in vivo. To our knowledge, this contribution to initiation of seizures is the first identified functional role for the TRPC7 ion channel.Fil: Phelan, K. D.. University of Arkansas for Medical Sciences; Estados UnidosFil: Shwe, U. T.. University of Arkansas for Medical Sciences; Estados UnidosFil: Abramowitz, J.. National Institute of Environmental Health Sciences; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Birnbaumer, Lutz. National Institute of Environmental Health Sciences; Estados UnidosFil: Zheng, F.. University of Arkansas for Medical Sciences; Estados Unido

Computation of equilibrium foam structure using the Surface Evolver

The Surface Evolver has been used to minimise the surface area of various ordered structures for monodisperse foam. Additional features have enabled its application to foams of arbitrary liquid fraction. Early results for the case of dry foam (negligible liquid fraction) produced a structure haveing lower surface area, or energy, than Kelvin\u27s 1887 minimal tetrakaidecahedron. The calculations reported here show that this remains the case when the liquid fraction is finite, up to about 11%, at which point an f.c.c arrangement of the cells becomes preferable

Virus Sharing, Genetic Sequencing, and Global Health Security

The WHO’s Pandemic Influenza Preparedness (PIP) Framework was a milestone global agreement designed to promote the international sharing of biological samples to develop vaccines, while that ensuring poorer countries would have access to those vaccines. Since the PIP Framework was negotiated, scientists have developed the capacity to use genetic sequencing data (GSD) to develop synthetic viruses rapidly for product development of life-saving technologies in a time-sensitive global emergency—threatening to unravel the Framework. Access to GSD may also have major implications for biosecurity, biosafety, and intellectual property (IP). By rendering the physical transfer of viruses antiquated, GSD may also undermine the effectiveness of the PIP Framework itself, with disproportionate impacts on poorer countries. We examine the changes that need to be made to the PIP Framework to address the growing likelihood that GSD might be shared instead of physical virus samples. We also propose that the international community harness this opportunity to expand the scope of the PIP Framework beyond only influenza viruses with pandemic potential. In light of non-influenza pandemic threats such as the Middle East Respiratory Syndrome (MERS) and Ebola, we call for an international agreement on the sharing of the benefits of research – such as vaccines and treatments – for other infectious diseases to ensure not only a more secure and healthy world, but also a more just world, for humanity

Redefining the performing arts archive

This paper investigates representations of performance and the role of the archive. Notions of record and archive are critically investigated, raising questions about applying traditional archival definitions to the performing arts. Defining the nature of performances is at the root of all difficulties regarding their representation. Performances are live events, so for many people the idea of recording them for posterity is inappropriate. The challenge of creating and curating representations of an ephemeral art form are explored and performance-specific concepts of record and archive are posited. An open model of archives, encouraging multiple representations and allowing for creative reuse and reinterpretation to keep the spirit of the performance alive, is envisaged as the future of the performing arts archive

Thermoelectric Properties of Intermetallic Semiconducting RuIn3 and Metallic IrIn3

Low temperature (<400 K) thermoelectric properties of semiconducting RuIn3 and metallic IrIn3 are reported. RuIn3 is a narrow band gap semiconductor with a large n-type Seebeck coefficient at room temperature (S(290K)~400 {\mu}V/K), but the thermoelectric Figure of merit (ZT(290K) = 0.007) is small because of high electrical resistivity and thermal conductivity ({\kappa}(290 K) ~ 2.0 W/m K). IrIn3 is a metal with low thermopower at room temperature (S(290K)~20 {\mu}V/K) . Iridium substitution on the ruthenium site has a dramatic effect on transport properties, which leads to a large improvement in the power factor and corresponding Figure of merit (ZT(380 K) = 0.053), improving the efficiency of the material by an over of magnitude.Comment: Submitted to JA

You’re the emotional one: the role of perspective for emotion processing in reading comprehension

Two experiments were conducted to explore whether perspective influences the way readers engage with and process emotional information while reading. Texts presenting characters in an emotional situation from either a personal or an onlooker perspective were presented and reading times were measured for each sentence. Participants also provided emotional self-ratings after reading. In the first experiment, positive texts were processed with greater ease, especially when readers experienced the texts from a personal perspective. In Experiment 2, an emotional match/mismatch was inserted so that a final explicit emotion word either matched or mismatched the emotional valence of the text. Mismatch effects were stronger and more consistent for the personal perspective. The two experiments provide evidence that the perspective of the reader can influence emotion processing. Processing of emotional information was easier for the personal perspective, and readers were more sensitive to inconsistent emotional information from that perspective

Initial Results From the First Field Expedition of UAPx to Study Unidentified Anomalous Phenomena

In July 2021, faculty from the UAlbany Department of Physics participated in a week-long field expedition with the organization UAPx to collect data on UAPs in Avalon, California, located on Catalina Island, and nearby. This paper reviews both the hardware and software techniques which this collaboration employed, and contains a frank discussion of the successes and failures, with a section about how to apply lessons learned to future expeditions. Both observable-light and infrared cameras were deployed, as well as sensors for other (non-EM) emissions. A pixel-subtraction method was augmented with other similarly simple methods to provide initial identification of objects in the sky and/or the sea crossing the cameras' fields of view. The first results will be presented based upon approximately one hour in total of triggered visible/night-vision-mode video and over 600 hours of untriggered (far) IR video recorded, as well as 55 hours of (background) radiation measurements. Following multiple explanatory resolutions of several ambiguities that were potentially anomalous at first, we focus on the primary remaining ambiguity captured at approximately 4am Pacific Time on Friday, July 16: a dark spot in the visible/near-IR camera possibly coincident with ionizing radiation that has thus far resisted a prosaic explanation. We conclude with quantitative suggestions for serious researchers in this still-nascent field of hard-science-based UAP studies, with an ultimate goal of identifying UAPs without confirmation bias toward either mundane or speculative conclusions.Comment: 43 pages, 16 figures, 2 tables, 18 equations, and 64 reference

A practical phase sensitive amplification scheme for two channel phase regeneration

A "black-box" phase sensitive amplifier is presented achieving simultaneous suppression of deterministic phase distortion on two independent 42.66 Gbit/s DPSK modulated signal wavelengths