What information could the main actors of liquid biopsy provide? A representative case of non-small cell lung cancer (NSCLC)

In non-small cell lung cancer (NSCLC), there is a consensus regarding the use of liquid biopsy, generally, to detect "druggable" mutations and, in particular, to monitor tyrosine kinase inhibitor (TKI) treatments. However, whether circulating tumor cells (CTCs) are better tools than cell-free DNA (cfDNA), is still a matter of debate, mainly concerning which antigen(s) we should use to investigating simultaneously both epithelial and epithelial-to-mesenchymal transient (EMT) phenotype in the same sample of CTCs. To address this item, we exploited here a single-tube liquid biopsy, to detect both epithelial cell adhesion molecule (EpCAM)-positive CTCs and EpCAM-low/negative CTCs, because down-modulation of EpCAM is considered the first step in EMT. Furthermore, we analyzed the DNA from CTCs of four different phenotypes (ctcDNA), according to their EpCAM expression and cytokeratin pattern, and circulating tumor DNA (ctDNA) by droplet digital PCR (ddPCR), in order to disclose activating and resistancedriving mutations. Liquid biopsy reflected spatial and temporal heterogeneity of the tumor under treatment pressure. We provide the proof-of-concept that the complementary use of ctDNA and ctcDNA represents a reliable, minimally invasive and dynamic tool for a more comprehensive view of tumor evolution

Metronomic Chemotherapy with Vinorelbine Produces Clinical Benefit and Low Toxicity in Frail Elderly Patients Affected by Advanced Non-Small Cell Lung Cancer

Lung cancer is the leading cause of death worldwide. The treatment choice for advanced stage of lung cancer may depend on histotype, performance status (PS), age, and comorbidities. In the present study, we focused on the effect of metronomic vinorelbine treatment in elderly patients with advanced unresectable non-small cell lung cancer (NSCLC). Methods. From January 2016 to December 2016, 44 patients affected by non-small cell lung cancer referred to our oncology day hospital were progressively analyzed. The patients were treated with oral vinorelbine 30 mg x 3/wk or 40 mg x 3/wk meaning one day on and one day off. The patients were older than 60, stage IIIB or IV, ECOG PS ≥ 1, and have at least one important comorbidity (renal, hepatic, or cardiovascular disease). The schedule was based on ECOG-PS and comorbidities. The primary endpoint was progression-free survival (PFS). PFS was used to compare patients based on different scheduled dosage (30 or 40 mg x3/weekly) and age (more or less than 75 years old) as exploratory analysis. We also evaluated as secondary endpoint toxicity according to Common Toxicity Criteria Version 2.0. Results. Vinorelbine showed a good safety profile at different doses taken orally and was effective in controlling cancer progression. The median overall survival (OS) was 12 months. The disease control rate (DCR) achieved 63%. The median PFS was 9 months. A significant difference in PFS was detected comparing patients aged below with those over 75, and the HR value was 0.72 (p<0.05). Not significant was the difference between groups with different schedules. Conclusions. This study confirmed the safety profile of metronomic vinorelbine and its applicability for patients unfit for standard chemotherapies and adds the possibility of considering this type of schedule not only for very elderly patients

EChO Payload electronics architecture and SW design

EChO is a three-modules (VNIR, SWIR, MWIR), highly integrated spectrometer, covering the wavelength range from 0.55 $\mu$m, to 11.0 $\mu$m. The baseline design includes the goal wavelength extension to 0.4 $\mu$m while an optional LWIR module extends the range to the goal wavelength of 16.0 $\mu$m. An Instrument Control Unit (ICU) is foreseen as the main electronic subsystem interfacing the spacecraft and collecting data from all the payload spectrometers modules. ICU is in charge of two main tasks: the overall payload control (Instrument Control Function) and the housekeepings and scientific data digital processing (Data Processing Function), including the lossless compression prior to store the science data to the Solid State Mass Memory of the Spacecraft. These two main tasks are accomplished thanks to the Payload On Board Software (P-OBSW) running on the ICU CPUs.Comment: Experimental Astronomy - EChO Special Issue 201

Echocardiography combined with cardiopulmonary exercise testing for the prediction of outcome in idiopathic pulmonary arterial hypertension

BACKGROUND: Right ventricular (RV) function is a major determinant of exercise intolerance and outcome in idiopathic pulmonary arterial hypertension (IPAH). The aim of the study was to evaluate the incremental prognostic value of echocardiography of the RV and cardiopulmonary exercise testing (CPET) on long-term prognosis in these patients. METHODS: One hundred-thirty treatment-naïve IPAH patients were enrolled and prospectively followed. Clinical worsening (CW) was defined by a reduction in 6-minute walk distance plus an increase in functional class, or non elective hospitalization for PAH, or death. Baseline evaluation included clinical, hemodynamic, echocardiographic and CPET variables. Cox regression modeling with c-statistic and bootstrapping validation methods were done. RESULTS: During a mean period of 528 ± 304 days, 54 patients experienced CW (53%). Among demographic, clinical and hemodynamic variables at catheterization, functional class and cardiac index were independent predictors of CW (Model-1). With addition of echocardiographic and CPET variables (Model-2), peak O2 pulse (peak VO2/heart rate) and RV fractional area change (RVFAC) independently improved the power of the prognostic model (AUC: 0.81 vs 0.66, respectively; p=0.005). Patients with low RVFAC and low O2 pulse (low RVFAC + low O2 pulse) and high RVFAC+low O2 pulse showed 99.8 and 29.4 increase in the hazard ratio, respectively (relative risk -RR- of 41.1 and 25.3, respectively), compared with high RVFAC+high O2 pulse (p=0.0001). CONCLUSIONS: Echocardiography combined with CPET provides relevant clinical and prognostic information. A combination of low RVFAC and low O2 pulse identifies patients at a particularly high risk of clinical deterioration

RELATÓRIO TÉCNICO DOS CRUZEIROS DO PROJETO VIEIRA. I. CRUZEIRO 1(4 A 9 DE DEZEMBRO DE 1995)

Main technical results and original data of Cruise I of the Scallop Project (December 4 to 9, 1995) are presented in this Report. Sampling sites were distributed along seven transects, between the 30 and 50 meters isobaths, according to information of previous surveys carried out in 1975. Salinity and temperature of the water column, and sediment samples were obtained at each sampling site for a physical characterisation of the scallop grounds. Two types of beam-trawl and one dredge were tested to catch scallops and benthic samples. Salinity and temperature showed an important gradient, with high temperatures and low salinities at surface waters, and low temperature and high salinities at bottom waters. Sediments were constituted by pure fine to very fine sands. Organic matter and calcium carbonate content, together with other statistical sediment parameters, showed a bathymetrical variation. Along the study area, two beds were found, at SSE of Bom Abrigo Island (São Paulo State) and E of São Francisco do Sul Island (Santa Catarina State), both between 36 and 40 m depth. Very low scallop catches were probably related to a total depletion of the original stock. Future cruises will try to determine the efficiencies of the beam-trawl for a real estimation of scallops densities.Neste relatório são apresentados os dados originais e principais resultados técnicos do Cruzeiro I do Projeto Vieira, realizado entre 4 e 9 de dezembro de 1995. As estações de amostragem foram distribuídas ao longo de 7 transversais entre as isóbatas de 30 e 50 m, em áreas de reconhecida ocorrência de vieiras no passado. Foram obtidos perfis verticais de temperatura e salinidade da água em todas as estações, bem como amostras de sedimento, visando uma caracterização física da região. Dois tipos de beam trawl e uma draga foram empregados na captura de vieira e amostras da macrofauna bentõnica. A temperatura e a salinidade mostraram um gradiente acentuado, com altas temperaturas e baixas salinidades em superfície, e baixas temperaturas e altas salinidades junto ao fundo. Os sedimentos são constituídos de areia fina a muito fina. Tanto os parâmetros granulométricos como o conteúdo de matéria orgânica e carbonato de cálcio mostraram um gradiente batimétrico em seus valores. Ao longo da área de estudo, foram encontrados dois bancos de vieira, um a SSE da Ilha do Bom Abrigo, no litoral sul de São Paulo, e outro a E da Ilha de São Francisco do Sul, no norte de Santa Catarina, ambos entre 36 e 40 metros de profundidade. Entretanto, as capturas em ambos os bancos foram reduzidas, indicando, provavelmente, a ocorrência de uma depleção total no estoque original. Em cruzeiros futuros, a eficiência de captura do beam trawl será determinada com o objetivo de realizar uma estimação real da densidade de vieiras nas áreas

Preliminary analysis of the genetic variability of two natural beds of the Scallop Euvola ziczac (Linnaeus, 1758) in Brazil

Euvola ziczac (formerly Pecten ziczac), a simultaneous hermaphroditic scallop was heavily fished in Brazil between 1972 and 1980. The production peaked in 1980 with 8,800 tons and was followed by the total collapse of the resource. In order to investigate the possible loss of genetic variability of the stock associated to overfishing and self-fertilization, the polymorphism of phosphoglucomutase (PGM) and glucose phosphate isomerase (GPI) was analyzed by electrophoresis of the adductor muscle of scallops from São Francisco (26° 20.583S; 48° 16.507W) and Bom Abrigo (25° 28.735S; 47° 37.621W) beds; the southern and northern extremes of the scallop fishing ground, respectively. Animals from São Francisco showed a strong deficiency of heterozygosity for GPI and PGM. In addition, PGM showed *exclusive alleles for each bed. Such results coupled with other information about the species suggested the following hypothesis: a) the stock was a metapopulation with at least two populations; b) some reproductive isolation might be occurring which might be influenced by conditions of larval transport and by the extremely low densities of scallops; c) presently, the stock seemed to be mostly maintained through self-fertilization; d) São Francisco could constitute a source-area, contributing with larvae and recruits to Bom Abrigo and other areas; e) both beds were suffering a genetic homogenization more evident in São Francisco. Such hypothesis needed to be investigated in order to furnish guidelines for future programs of recovery and management of the resource.info:eu-repo/semantics/publishedVersio

Transverse orange nail lesions following SARS-CoV-2 infection

We report the case of an 89-year-old woman in a nursing home who amid an outbreak of coronavirus disease 2019 (COVID-19) presented cough and asthenia

Characterizing the structure of diffuse emission in Hi-GAL maps

We present a study of the structure of the Galactic interstellar medium through the Delta-variance technique, related to the power spectrum and the fractal properties of infrared/sub-mm maps. Through this method, it is possible to provide quantitative parameters which are useful to characterize different morphological and physical conditions, and to better constrain the theoretical models. In this respect, the Herschel Infrared Galactic Plane Survey carried out at five photometric bands from 70 to 500 \mu m constitutes an unique database for applying statistical tools to a variety of regions across the Milky Way. In this paper, we derive a robust estimate of the power-law portion of the power spectrum of four contiguous 2{\deg}x2{\deg} Hi-GAL tiles located in the third Galactic quadrant (217{\deg} < l < 225{\deg}, -2{\deg} < b < 0{\deg}). The low level of confusion along the line of sight testified by CO observations makes this region an ideal case. We find very different values of the power spectrum slope from tile to tile but also from wavelength to wavelength (2 < \beta < 3), with similarities between fields attributable to components located at the same distance. Thanks to the comparison with models of turbulence, an explanation of the determined slopes in terms of the fractal geometry is also provided, and possible relations with the underlying physics are investigated. In particular, an anti-correlation between ISM fractal dimension and star formation efficiency is found for the two main distance components observed in these fields. A possible link between the fractal properties of the diffuse emission and the resulting clump mass function is discussed.Comment: Accepted by Ap

The pre-ZAMS nature of Mol160/IRAS23385+6053 confirmed by Spitzer

Determining the timeline for the formation of massive YSOs requires the identification and characterisation of all the phases that a massive forming YSO undergoes. It is of particular interest to verify the observability of the phase in which the object is rapidly accreting while not yet igniting the fusion of hydrogen that marks the arrival on the ZAMS. One of the candidate prototypical objects for this phase is Mol160/IRAS23385+6053, which previous studies suggest it could be in a pre-Hot Core stage. We further investigate this issue by means of Spitzer imaging and spectroscopy in the 5-70 micron range. The dense core of Mol160/IRAS23385+6053, which up to now had only been detected at submm and mm wavelenghts has been revealed for the first time at 24 and 70 micron by Spitzer. The complete 24 micron -3.4 mm continuum cannot be fitted with a standard model of a Zero-Age Main-Sequence (ZAMS) star embedded in an envelope. A simple greybody fit yields a mass of 220 solar masses. The luminosity is slightly in excess of 3000 solar luminosities, which is a factor of 5 less than previous estimates when only IRAS fluxes were available between 20 and 100 micron. The source is under-luminous by the same factor with respect to UCHII regions or Hot-Cores of similar circumstellar mass, and simple models show that this is compatible with an earlier evolutionary stage. Spectroscopy between 5 and 40 microns revelas typical PDR/PIR conditions, where the required UV illumination may be provided by other sources revealed at 24 microns in the same region, and which can be plausibly modeled as moderately embedded intermediate-mass ZAMS stars. Our results strengthen the suggestion that the central core in Mol160/IRAS23385+6053 is a massive YSO actively accreting from its circumstellar envelope and which did not yet begin hydrogen fusion.Comment: Accepted by A&