Anotação funcional da família gênica Dof no genoma da videira.

Resumo

Molecular and metabolic mechanisms associated with fleshy fruit quality

Funding to AF was provided by the Portuguese Foundation for Science and Technology (FCT Investigator IF/00169/2015, PEst-OE/BIA/UI4046/2014). Research in the AG lab was supported by the EC H2020 Program:TRADITOM-634561 and TOMGEM679796 and networking activities by COST FA1106.Fortes, AM.; Granell Richart, A.; Pezzotti, M.; Bouzayen, M. (2017). Molecular and metabolic mechanisms associated with fleshy fruit quality. Frontiers in Plant Science. 8:6-10. https://doi.org/10.3389/fpls.2017.01236S6108Agudelo-Romero, P., Erban, A., Sousa, L., Pais, M. S., Kopka, J., & Fortes, A. M. (2013). Search for Transcriptional and Metabolic Markers of Grape Pre-Ripening and Ripening and Insights into Specific Aroma Development in Three Portuguese Cultivars. PLoS ONE, 8(4), e60422. doi:10.1371/journal.pone.0060422Fortes, A. M., & Gallusci, P. (2017). Plant Stress Responses and Phenotypic Plasticity in the Epigenomics Era: Perspectives on the Grapevine Scenario, a Model for Perennial Crop Plants. Frontiers in Plant Science, 08. doi:10.3389/fpls.2017.00082Fortes, A., Teixeira, R., & Agudelo-Romero, P. (2015). Complex Interplay of Hormonal Signals during Grape Berry Ripening. Molecules, 20(5), 9326-9343. doi:10.3390/molecules20059326Liu, R., How-Kit, A., Stammitti, L., Teyssier, E., Rolin, D., Mortain-Bertrand, A., … Gallusci, P. (2015). A DEMETER-like DNA demethylase governs tomato fruit ripening. Proceedings of the National Academy of Sciences, 112(34), 10804-10809. doi:10.1073/pnas.150336211

Semiclassical Propagation of Coherent States for the Hartree equation

In this paper we consider the nonlinear Hartree equation in presence of a given external potential, for an initial coherent state. Under suitable smoothness assumptions, we approximate the solution in terms of a time dependent coherent state, whose phase and amplitude can be determined by a classical flow. The error can be estimated in $L^2$ by C \sqrt {\var}, \var being the Planck constant. Finally we present a full formal asymptotic expansion

Regeneration of plants from embryogenic callus-derived protoplasts of Garganega and Sangiovese grapevine (Vitis vinifera L.) cultivars

Protoplasts are useful research tools for basic and applied plant science, but the regeneration of whole plants from protoplasts is challenging in most of agronomically important crops, including grapevine (Vitis vinifera L.). Here we describe an efficient protocol for the induction of embryogenic callus, the isolation of protoplasts, and the regeneration of whole grapevine plants in two Italian grapevine cultivars. Embryogenic callus was induced successfully from stamens collected from immature flowers. Isolated protoplasts were tested to confirm their viability and then cultivated using the disc-culture method, at a density of 1\u2009 7\u2009105 protoplasts/mL in solid Nitsch\u2019s medium supplemented with 2 mg/L 1-naphthaleneacetic acid and 0.5 mg/L 6-benzylaminopurine. After 3\u20134 months, the protoplasts of both cultivars regenerated with similar efficiency into cotyledonal-stage somatic embryos. The somatic embryos were transferred to solid Nitsch\u2019s medium supplemented with 30 g/L sucrose and 2 g/L gellan gum, and were maintained in the dark for 4 weeks. This step was necessary for the embryo to complete germination, allowing subsequent shoot elongation in response to light on a medium with 4 \ub5M 6-benzylaminopurine. Then root elongation occurred after transferring on a medium with 0.5 \ub5M 1-naphthaleneacetic. After\u2009~\u20096 months from the isolation of protoplasts, normal plants were regenerated, which were moved to the greenhouse. The protoplasts could also be transfected using the polyethylene glycol method, as confirmed using a plasmid carrying the yellow florescent protein marker gene. The new method is therefore compatible with biotechnological applications such as gene transfer and genome editing

Raman tensor analysis of hexagonal polyoxymethylene and its application to study the molecular arrangement in highly crystalline electrospun nanofibers

The orientation dependence in space of Raman-active vibrations in the hexagonal structure of polyoxymethylene (POM) is discussed in terms of Raman tensor elements as intrinsic physical parameters of the lattice. The variation of polarized intensity for the A1 and the E1 vibrational modes with respect to the POM molecular orientation is systematically studied, from both theoretical and experimental viewpoints, according to the symmetry assignments of each vibrational mode. A set of working equations including the Raman selection rules associated with the A1 and the E1 modes and the orientation distribution function are explicitly formulated and validated by means of a least-square fitting procedure on experimental data. In addition, an approach based on the introduction of orientation distribution functions is applied to quantitatively assess and compare on a statistical base the molecular orientation of two different types of electrospun POM nanofibers. Copyright (c) 2012 John Wiley & Sons, Ltd

Diseño de un instrumento portátil para aplicaciones ambientales por mediciones amperométricas sobre material biológico

El instrumento portátil optimizado para las medidas  de amperometria, en el monitoreo de  materiales bioactivos ha sido diseñado, fabricado y probado. Expresamente ha sido diseñado, para funcionar con una amplia gama de bio-muestras foto-activas. La cámara  de medición del instrumento; destaca dos tipos de fuentes ópticas para detectar la actividad fotosintética de plantas (p. ej. spinacia oleracea) y microorganismos (p. ej. algas y cyanobacteria). En la cámara son inseridos los electrodos serigrafiados para medir la corriente fotogenerada, ademas cuenta con un sistema de flujo para el transporte del electrólito. La transferencia fotosintética de electrones, es activada por dos LEDs (470nm y 660nm de emisión), para permitir varias longitudes de onda de excitación para utilizarlos con diversos materiales biológicos. El objetivo de la aplicacion, es en campos como agroalimentario, farmacéutico y biomédico. Este Artículo describe algunas de las posibles aplicaciones ambientales ABSTRACT A portable instrument performing amperometric measurements for monitoring bioactive materials has been designed, manufactured and tested. It has been specifically designed to operate with a wide range of photoactive biosamples. The sensing chamber in the instrument features two different optical sources to detect the photosynthetical activity of plants (i.e. spinacia oleracea) and microorganisms (i.e. algae and cyanobacteria). The chamber is provided with screen-printed electrodes to measure the photogenerated current and with a fluidic system for the electrolyte transport. Photosynthetic electron transfer is activated by two LEDs (470nm and 660nm emission) in order to enable various excitation wavelengths and match several different biological materials. Target applications belong to the agro-food, pharmaceutical and biomedical fields. This paper describes some possible environmental application

Análise global da expressão de genes Dof em videira.

Neste trabalho é caracterizada a família gênica Dof em videira (Vitis vinifera L.), a qual é composta por 25 genes. A busca por domínios conservados foi realizada utilizando-se o programa MEME suite v.4.9.0. Estudos filogenéticos foram conduzidos comparando 163 sequências Dof de videira, álamo, Arabidopsis thaliana e tomate com proteínas Dof de função conhecida previamente descritos na literatura