The stability of olive leaf drink during storage at room temperature

Cilj ovog rada je praćenje stabilnosti napitka od lista masline tijekom tromjesečnog skladištenja na sobnoj temperaturi određivanjem ukupnih fenola, antioksidacijske aktivnosti, boje, senzorskih svojstava te mikrobiološke kakvoće. Ukupni fenoli su određivani Folin – Ciocalteu metodom, a antioksidacijska aktivnost DPPH metodom. Boja se mjerila po CIELAB sustavu, a za određivanje senzorskih svojstava provedena je kvantitativna deskriptivna analiza. Udio ukupnih fenola u napitku kreće se od 0,276 mg GAE/mL do 0,186 mg GAE/mL, a tijekom skladištenja nije uočen jedinstveni trend promjena. Isto se odnosi i na antioksidacijsku aktivnost napitka samo se ona kretala u vrlo uskom rasponu od 0,144 – 0,149 μmol TAE/mL. Senzorska analiza ukazuje na vrlo male promjene senzorskih svojstava napitka. Skladištenjem se povećava ukupna razlika obojenosti. Mikrobiološka analiza je pokazala prisutnost aerobnih mezofilnih bakterija iznad dopuštene granice, ali stabilnost tijekom skladištenja.The purpose of this work is to monitor the stability of olive leaf drink during three months storage at room temperature by determining total phenol content, antioxidant activity, color, sensory properties and microbiological quality. Total phenols were determinated by Folin – Ciocalteu method and antioxidant activity by DPPH method. The color was measured by the CIELAB system and quantitative descriptive analysis was used to evaluate the sensory properties. The percentage of total phenol in the drink ranges from 0,276 mg GAE/mL to 0,186 mg GAE/mL and no unique change trend has been observed during storage. The same applies to the antioxidant activity of drink only in a very narrow range of 0,144 – 0,149 μmol TAE/mL. Storage incereases the overall color difference. Microbiological analysis showed the presence of aerobic mesophilic bacteria above the allowed limit, but also the stability during storage

Circular economy in the context of sustainable development

Kružna ekonomija predstavlja model proizvodnje i potrošnje koji uključuje ponovno korištenje, prenamjenu ili recikliranje postojećih proizvoda kako bi im se produžio životni vijek te istovremeno smanjili troškovi proizvodnje, kao i količina otpada nastala prilikom proizvodnih procesa i/ili uporabe proizvoda. Suprotnost kružnoj ekonomiji model je linearne ekonomije koji je potrebno što prije zamijeniti održivim načinom proizvodnje, naročito u poljoprivredi. Poljoprivreda je drugi po redu najveći zagađivač i proizvođač stakleničkih plinova, a uporabljivost otpada u toj djelatnosti i dalje je nedovoljno iskorištena. Mineralna gnojiva, pesticidi, herbicidi i druga zaštitna sredstva samo su neki od preparata koji su neophodni za ostvarivanje što većih prinosa i prihoda, no dugoročno donose negativne učinke na tlo, faunu i zdravlje čovjeka. Cilj rada je dati pregled pojmova povezanih s konceptom kružne ekonomije i održivim razvojem te provesti empirijsko istraživanje radi utvrđivanja mišljenja ispitanika o potencijalima primjene kružnog gospodarenja u poljoprivrednoj proizvodnji. Anketnim ispitivanje obuhvatilo se 25 poljoprivrednih proizvođača s područja Osječko-baranjske županije. Skoro polovica ispitanika (46 %) odgovorila je kako ima od 2 do 5 nusproizvoda prilikom proizvodnje, a 32 % ispitanika odgovorilo je da ih nema. Samo jedan ispitanik odgovorio je da ima više od 5 nusproizvoda. Najučestalija potrošnja nusproizvoda identificirana je kao organsko gnojivo, zatim kao prehrana za stoku, kao ogrjev ili za recikliranje. Primjenom inovativnosti, znanja i tehnologije moguće je utjecati na nove proizvodne modele koji će potaknuti brigu o okolišu, ali i stvarati nova radna mjesta te povećati konkurentnost na tržištu.The circular economy is a model of production and consumption that includes the reuse, conversion or recycling of existing products to extend their life and at the same time reduce production costs and the amount of waste generated during production processes and / or use of products. The opposite of a circular economy is a model of a linear economy that needs to be replaced as soon as possible by a sustainable mode of production, especially in agriculture. Agriculture is the second largest polluter and producer of greenhouse gases, and the usability of waste in this activity is still underutilized. Mineral fertilizers, pesticides, herbicides and other protective agents are just some of the preparations that are necessary to achieve the highest possible yields and income, but in the long run, bring negative effects on soil, fauna and human health. The aim of the paper was to provide an overview of concepts related to the concept of circular economy and sustainable development and to conduct empirical research to determine the attitudes and opinions of respondents on the potential of circular management in agricultural production. 25 agricultural producers from the area of Osijek-Baranja County were tested with the original measuring instrument. Almost half of the respondents answered that they have a couple of by-products during production, and 32% of the respondents answered that there are none. Only one respondent answered that there are more than 5 by-products. The most common consumption of by-products was identified as organic fertilizer, followed by livestock feed, firewood or recycling. By applying innovation, knowledge and technology, it is possible to influence new production models that will encourage care for the environment but also create new jobs and increase competitiveness in the market

Stabilnost napitka od maslinovog lista ovisno o skladištenju na različitim temperaturama

In the olive oil production, olive leaves mostly often remain insufficiently used and represent waste, although many studies showed its anticancer, antiinflammatory and antimicrobial properties due to abundance of polyphenols, which makes olive leaves potential ingredient for functional food production. Also, olive leaf drink due to it valuable composition represents functional drink itself. But, functional properties can reduce due to polyphenols degradation, especially during storage. Hence, this research aimed to investigate the stability of olive leaf drink during nine weeks storage at different temperatures (8, 22 and 32 °C). Influence of storage time and storage temperature was monitored by analysis of total phenols, antioxidant capacity by DPPH, color parameters (L*, a*, b*, , BI) and sensory properties of olive leaf drink samples. Obtained results showed that total phenols slightly decreased during storage, being most stabile on 8 °C. Antioxidant capacity showed approximately similar trend. Color of all samples darkened by the storage with increase rate of L*, and BI related to temperature increase. Major color changes were observed by the storage ending at 32 °C. Sensory analysis confirmed change of olive leaf drink color, where yellowness decreased as brownness increased by the storage time and temperature. Odor and flavor decreased with time and at higher storage temperatures. Intensity of drink\u27s main taste attributes (astringent and bitter taste) decreased by storage time, especially on higher temperatures, resulting with higher scored harmony taste. Obtained results showed good stability of olive leaf drink, suggesting its potential as a functional product or semi-product.U proizvodnji maslinovog ulja, maslinovo lišće najčešće ostaje nedovoljno upotrebljeno i predstavlja otpad, iako su mnoge studije pokazale njegova antikancerogena, protuupalna i antimikrobna svojstva zbog obilja polifenola, što ga čini potencijalnim sastojkom za proizvodnju funkcionalne hrane. Također, napitak od maslinovog lista predstavlja samostalno funkcionalno piće zbog njegovog vrijednog sastava. No, funkcionalna svojstva se, osobito tijekom skladištenja, mogu smanjiti zbog degradacije polifenola. Stoga je ovo istraživanje imalo za cilj istražiti stabilnost napitka od maslinovog lista tijekom devet tjedana skladištenja na različitim temperaturama (8, 22 i 32 °C). Utjecaj vremena i temperature skladištenja ispitan je određivanjem ukupnih fenola, antioksidacijskog kapaciteta DPPH metodom, parametara boje (L*, a*, b*, , BI) i senzorskih svojstava napitka od maslinovog lista. Dobiveni rezultati pokazali su da se ukupni fenoli tijekom skladištenja neznatno smanjuju, pokazujući najveću stabilnost na 8 °C. Antioksidacijski kapacitet pokazao je približno isti trend. Boja svih uzoraka je skladištenjem potamnila uz porast L*, i BI povećanjem temperature. Najveće promjene boje zabilježene su na 32 °C krajem skladištenja. Senzorska analiza potvrdila je promjenu boje napitka od maslinovog lista smanjenjem žute te povećanjem smeđe boje s vremenom i temperaturom skladištenja. Miris i aroma su se s vremenom i na višoj temperaturi smanjili. Intenzitet glavnih svojstava okusa napitka (trpak i gorak okus) se za vijeme skladištenja smanjio, osobito na višim temperaturama, što je rezultiralo više ocijenjenom harmoničnosti okusa. Dobiveni rezultati pokazali su dobru stabilnost napitka od maslinovog lista ukazujući na njegov potencijal kao funkcionalnog proizvoda ili poluproizvoda

Short term outcomes in the elderly patients with non-ST-elevation acute coronary syndromes undergoing early percutaneous coronary intervention: a report from the ISACS-TC registry

n/

Fine-Needle Aspiration Cytology of Apocrine Hidradenoma

An apocrine hidradenoma is a benign adnexal neoplasm, usually covered by intact skin, but may show superficial ulceration and serous discharge. This feature is raising the possibility of malignancy as it was in our case of macroscopically suspicious tumour. We described cytomorphologic features of cutaneous nodule that might be a lead to the cytologic diagnosis of hidradenoma, but primary or secondary malignant tumour has been ruled out first

Characterization of patients with myocardial infarction with non-obstructive coronary arteries – experience from the Croatian branch of the ISACS-CT Registry

Background and Aim: There are still wide knowledge gaps in myocardial infarction with non-obstructive coronary arteries (MINOCA) - a heterogeneous entity seen in 1-10% of patients with acute coronary syndrome (ACS)1. The aim is to determine characteristics of MINOCA in the Croatian branch of the ISACS-CT registry, and compare them to age- and gender-matched patients with unstable angina (UA), non-ST-segment elevation myocardial infarction (NSTEMI) and ST segment elevation myocardial infarction (STEMI). Patients and Methods: The study included 2487 patients with ACS. MINOCA was defined by simultaneous cardiac troponin levels >0.014 ng/L, symptoms of ischemia or significant ST-T changes in the ECG, and an absence of coronary artery stenosis of ≥ 50% on angiography. Age and gender-matching was performed from the remaining cohort by randomly sampling patients from the UA, STEMI and NSTEMI subgroups, based on the mean age ± 5 years and the gender ratio of the MINOCA group. Results: MINOCA was seen in 2.5% (n=63) of the cohort, initially categorized as UA (37%), NSTEMI (48%) and STEMI (16%). Median age was 62 (53, 71) years, 56% male. After age- and gender-matching, there were 36 UA (10% of the UA cohort), 135 NSTEMI (15%) and 198 STEMI (16%) patients in the ACS control subgroups. MINOCA patients had a lower prevalence of diabetes, hypertension, dyslipidemia, chronic kidney disease and tobacco use as compared to UA and NSTEMI. MINOCA patients used less antiplatelets, beta-blockers and statins before hospitalization. MINOCA and STEMI subgroups had a high incidence of chest pain symptoms and a short time from symptom onset to hospitalization. In the first 24h of hospitalization, less MINOCA patients were treated with antiplatelets and statins, and at discharge, they were less frequently prescribed with antiplatelet drugs compared to UA and NSTEMI (Table 1). Inhospital mortality was low, with no deaths in MINOCA and UA patients, and 2 and 3 deaths in NSTEMI and STEMI, respectively. Conclusion: MINOCA patients are mainly categorized as UA and NSTEMI at presentation, but have less comorbidities, more pronounced symptoms of typical chest pain, a shorter time from symptoms to hospitalization, lower levels of statin and antiaggregation prescription at admission, and antiaggregation at discharge. In-hospital mortality confirms MINOCA as low risk, however, long-term registry follow-up is needed to learn about longer term outcomes

Characteristics of patients with acute ST-segment elevation myocardial infarction treated with different combinations of antiaggregation therapy: experience from the Croatian branch of the ISACS-CT Registry

Background and Aim: The relevance of dual antiplatelet therapy (DAPT) in acute ST-segment elevation myocardial infarction (STEMI) is well-established (aspirin and P2Y12 inhibitors).1 The role of glycoprotein (GP) IIb/IIIa inhibitors in clinical practice is not completely defined. Administration in the event of thrombotic complications is considered reasonable, although there is no evidence for routine use in primary percutaneous coronary intervention (pPCI). The aim was to analyze early outcomes of STEMI patients (pts) in the Croatian branch of the ISACS-CT (International Registry of Acute Coronary Syndromes in Transitional Countries) registry, depending on received antiaggregation therapy. Patients and Methods: Data were gathered retrospectively from pts hospitalized between January 2012 to October 2017. The study included 2503 pts with acute coronary syndrome, from which 48.9% (n=1224) were diagnosed with STEMI. The patients were divided into 4 groups depending on administered antiaggregation therapy. Results: For 7.8% (n=96) pts antiaggregation therapy data were missing, and 5.8% (n=71) were not treated with DAPT. Remaining 1057 (86.4%) pts were analyzed. Aspirin was administered in 95% of pts in the first 24 hours. 41.9% (n=443) of pts were additionally treated with clopidogrel, 16.1% (n=170) with ticagrelor, 28.6% (n=302) with clopidogrel and eptifibatide, and 13.4% (n=142) with ticagrelor and eptifibatide (Table 1). The groups did not differ in comorbidities, while pts receiving eptifibatide had lower systolic blood pressure on admission. Patients treated with eptifibatide were more frequently male, smokers, of younger age, had more thrombotic complications seen on coronary angiography (predominantly distal embolisation and “no-reflow” phenomenon) and lower in-hospital mortality. In a multivariable regression model adjusted for age, gender, hypertension, diabetes, and pPCI, increasing age (OR=1.1), diabetes (OR=1.9) and pPCI (OR=0.5) remained relevant to in-hospital mortality. Conclusion: STEMI patients that are young, male and smokers are more frequently treated with eptifibatide, likely due to a higher burden of thrombotic complications. Unlike the choice of antiaggregation therapy, increasing age, diabetes and non-invasive management of STEMI were associated with in-hospital mortality

With food to health : proceedings of the 10th International scientific and professional conference

Proceedings contains 13 original scientific papers, 10 professional papers and 2 review papers which were presented at "10th International Scientific and Professional Conference WITH FOOD TO HEALTH", organised in following sections: Nutrition, Dietetics and diet therapy, Functional food and food supplemnents, Food safety, Food analysis, Production of safe food and food with added nutritional value

Water for all : Proceedings of the 7th international scientific and professional conference Water for all

The 7th International Scientific and Professional Conference Water for all is organized to honour the World Water Day by the Josip Juraj Strossmayer University of Osijek, European Hygienic Engineering & Design Group (EHEDG), Danube Parks, Croatian Food Agency, Croatian Water, Faculty of Food Technology Osijek, Faculty of Agriculture in Osijek, Faculty of Civil Engineering Osijek, Josip Juraj Strossmayer University of Osijek Department of Biology, Josip Juraj Strossmayer University of Osijek Department of Chemistry, Nature Park “Kopački rit”, Osijek- Baranja County, Public Health Institute of the Osijek- Baranja County and „Vodovod-Osijek“ -water supply company in Osijek. The topic of World Water Day 2017 was "Wastewater" emphasizing the importance and influence of wastewater treatments on global environment. The international scientific and professional conference Water for all is a gathering of scientists and experts in the field of water management, including chemists, biologists, civil and agriculture engineers, with a goal to remind people about the significance of fresh water and to promote an interdisciplinary approach and sustainability for fresh water resource management. The Conference has been held since 2011. About 300 scientists and engineers submitted 95 abstracts to the 7th International Scientific and Professional Conference Water for all, out of which 33 was presented orally and 62 as posters. 47 full papers were accepted by the Scientific Committee. 38 full papers became the part of the this Proceedings while 9 papers were accepted for publication in Croatian Journal of Food Science and Technology and Electronic Journal of the Faculty of Civil Engineering Osijek - e-GFOS

Optimization of Ultrasound-Assisted Extraction of Pigments from Green Macroalga Codium bursa

Provedena je ekstrakcija pigmenata potpomognuta ultrazvukom (UAE) iz zelene makroalge Codium bursa s ciljem optimiranja parametara ekstrakcije. Uz metanol:diklormetan kao ekstrakcijsko otapalo, ispitivani su sljedeći uvjeti ekstrakcije: temperatura (30, 50 i 70 °C), vrijeme (15, 30 i 45 min) i omjer tekuće:kruto (10, 20 i 30 mL/g). Temeljem metode odzivnih površina, provedeno je 17 pokusa pri čemu je praćen utjecaj procesnih parametara na udjele ispitivanih pigmenata. Koncentracije fukoksantina u dobivenim ekstraktima određene su visokodjelotvornom tekućinskom kromatografijom (HPLC) te su iznosile od 0,07 do 0,43 mg/g suhog ekstrakta i bile su više nego u ekstraktima dobivenim konvencionalnom metodom (0,18 ± 0,09 mg/g suhog ekstrakta). Statistički visoko značajan utjecaj na ekstrakciju fukoksantina imali su linearan i kvadratni član vremena, a njegova najveća koncentracija je dobivena pri uvjetima: 30,29 °C, 15,06 min, 15,17 mL/g. Preostali pigmenti određeni su spektrofotometrijski, a utvrđene koncentracije bile su od 0,0336 do 0,3762 mg/g suhog ekstrakta za klorofil b, 0,0067 do 0,0685 mg/g suhog ekstrakta za klorofil a te 0,0038 do 0,0467 mg/g suhog ekstrakta za ukupne karotenoide. Statistički visoko značajan utjecaj na ekstraktabilnost klorofila b i ukupnih karotenoida imali su linearni član temperature i omjer tekuće:kruto, a za klorofil a su uz te parametre značajan utjecaj imali i drugi. Njihove najveće koncentracije dobivene su pri sljedećim uvjetima: 30°C, 30 min i 10 mL/g.Ultrasound-assisted extraction of pigments (UAE) from the green macroalga Codium bursa was performed with the aim of optimizing the extraction parameters. With methanol:dichloromethane as extraction solvent, the following conditions were investigated: temperature (30, 50 and 70 °C), time (15, 30 and 45 min) and solvent:solid ratio (10, 20 and 30 mL/g). Based on response surface methodology, 17 experiments were performed, in which the influence of process parameters on concentrations of tested pigments was monitored. Fucoxanthin concentrations in obtained extracts were determined by high-performance liquid chromatography (HPLC) and ranged from 0.07 to 0.43 mg/g of dry extract and were higher than in the extracts obtained by the conventional method (0.18 ± 0.09 mg/g dry extract). Linear and quadratic terms of time had statistically highly significant influence on fucoxanthin extraction, and its highest concentration was obtained at the following conditions: 30.29 °C, 15.06 min, 15.17 mL/g. Concentrations of other pigments were determined spectrofotometrically and ranged from 0.0336 to 0.3762 mg/g for chlorophyll b, 0.0067 to 0.0685 mg/g for chlorophyll a, and 0.0038 to 0. 0467 mg/g dry extract for total carotenoids. Linear term of temperature and solvent:solid ratio had statistically highly significant influence on extractability of chlorophyll b and total carotenoids; for chlorophyll a those and other parameters had a significant influence. Their highest concentrations were obtained at the conditions: 30°C, 30 min and 10 mL/g