Aeroelastic loads and stability investigation of a full-scale hingeless rotor

An analytical investigation was conducted to study the influence of various parameters on predicting the aeroelastic loads and stability of a full-scale hingeless rotor in hover and forward flight. The CAMRAD/JA (Comprehensive Analytical Model of Rotorcraft Aerodynamics and Dynamics, Johnson Aeronautics) analysis code is used to obtain the analytical predictions. Data are presented for rotor blade bending and torsional moments as well as inplane damping data obtained for rotor operation in hover at a constant rotor rotational speed of 425 rpm and thrust coefficients between 0.0 and 0.12. Experimental data are presented from a test in the wind tunnel. Validation of the rotor system structural model with experimental rotor blade loads data shows excellent correlation with analytical results. Using this analysis, the influence of different aerodynamic inflow models, the number of generalized blade and body degrees of freedom, and the control-system stiffness at predicted stability levels are shown. Forward flight predictions of the BO-105 rotor system for 1-G thrust conditions at advance ratios of 0.0 to 0.35 are presented. The influence of different aerodynamic inflow models, dynamic inflow models and shaft angle variations on predicted stability levels are shown as a function of advance ratio

Full-scale hingeless rotor performance and loads

A full-scale BO-105 hingeless rotor system was tested in the NASA Ames 40- by 80-Foot Wind Tunnel on the rotor test apparatus. Rotor performance, rotor loads, and aeroelastic stability as functions of both collective and cyclic pitch, tunnel velocity, and shaft angle were investigated. This test was performed in support of the Rotor Data Correlation Task under the U.S. Army/German Memorandum of Understanding on Cooperative Research in the Field of Helicopter Aeromechanics. The primary objective of this test program was to create a data base for full-scale hingeless rotor performance and structural blade loads. A secondary objective was to investigate the ability to match flight test conditions in the wind tunnel. This data base can be used for the experimental and analytical studies of hingeless rotor systems over large variations in rotor thrust and tunnel velocity. Rotor performance and structural loads for tunnel velocities from hover to 170 knots and thrust coefficients (C(sub T)/sigma) from 0.0 to 0.12 are presented in this report. Thrust sweeps at tunnel velocities of 10, 20, and 30 knots are also included in this data set

Dynamic Calibration of the NASA Ames Rotor Test Apparatus Steady/Dynamic Rotor Balance

The NASA Ames Rotor Test Apparatus was modified to include a Steady/Dynamic Rotor Balance. The dynamic calibration procedures and configurations are discussed. Random excitation was applied at the rotor hub, and vibratory force and moment responses were measured on the steady/dynamic rotor balance. Transfer functions were computed using the load cell data and the vibratory force and moment responses from the rotor balance. Calibration results showing the influence of frequency bandwidth, hub mass, rotor RPM, thrust preload, and dynamic loads through the stationary push rods are presented and discussed

Dynamic response of NASA Rotor Test Apparatus and Sikorsky S-76 hub mounted in the 80- by 120-Foot Wind Tunnel

A shake test was conducted in the 80- by 120-Foot Wind Tunnel at NASA Ames Research Center, using the NASA Ames Rotor Test Apparatus (RTA) and the Sikorsky S-76 rotor hub. The primary objective of this shake test was to determine the modal properties of the RTA, the S-76 rotor hub, and the model support system installed in the wind tunnel. Random excitation was applied at the rotor hub, and vibration responses were measured using accelerometers mounted at various critical locations on the model and the model support system. Transfer functions were computed using the load cell data and the accelerometer responses. The transfer function data were used to compute the system modal parameters with the aid of modal analysis software

Leading Organizational Change: A Comparison of County and Campus Views of Extension Engagement

Feeling pressure from stakeholders, Cornell Cooperative Extension has responded using the Kellogg Commission report on the Future of State and Land-Grant Universities as a blueprint for organizational change. Results of interviews revealed both similarities and differences in county and state staff views of engagement. The most significant difference is that county staff view their jobs as the critical link between community needs and university resources, while campus staff view them as implementers of programs. This finding suggests a need to engage all staff in a mutual dialogue of roles and expectation if change based on Extension engagement is to be successful

Time-averaged aerodynamic loads on the vane sets of the 40- by 80-foot and 80- by 120-foot wind tunnel complex

Time-averaged aerodynamic loads are estimated for each of the vane sets in the National Full-Scale Aerodynamic Complex (NFAC). The methods used to compute global and local loads are presented. Experimental inputs used to calculate these loads are based primarily on data obtained from tests conducted in the NFAC 1/10-Scale Vane-Set Test Facility and from tests conducted in the NFAC 1/50-Scale Facility. For those vane sets located directly downstream of either the 40- by 80-ft test section or the 80- by 120-ft test section, aerodynamic loads caused by the impingement of model-generated wake vortices and model-generated jet and propeller wakes are also estimated

Heating the hot atmospheres of galaxy groups and clusters with cavities: the relationship between jet power and low-frequency radio emission

We present scaling relations between jet power and radio power measured using the Giant Metrewave Radio Telescope (GMRT), Chandra and XMM-Newton, for a sample of 9 galaxy groups combined with the Birzan et al. sample of clusters. Cavity power is used as a proxy for mechanical jet power. Radio power is measured at 235 MHz and 1.4 GHz, and the integrated 10 MHz-10 GHz radio luminosity is estimated from the GMRT 610-235 MHz spectral index. The use of consistently analysed, high resolution low-frequency radio data from a single observatory makes the radio powers for the groups more reliable than those used by previous studies, and the combined sample covers 6-7 decades in radio power and 5 decades in cavity power. We find a relation of the form Pjet proportional to Lradio^~0.7 for integrated radio luminosity, with a total scatter of sigma_Lrad=0.63 and an intrinsic scatter of sigma_i,Lrad=0.59. A similar relation is found for 235 MHz power, but a slightly flatter relation with greater scatter is found for 1.4 GHz power, suggesting that low-frequency or broad band radio measurements are superior jet power indicators. We find our low-frequency relations to be in good agreement with previous observational results. Comparison with jet models shows reasonable agreement, which may be improved if radio sources have a significant low-energy electron population. We consider possible factors which could bias our results or render them more uncertain, and find that correcting for such factors in those groups we are able to study in detail leads to a flattening of the Pjet:Lradio relation.Comment: Accepted for publication in ApJ, 7 pages, 3 figure

Generation of orthotopic patient-derived xenografts from gastrointestinal stromal tumor.

BackgroundGastrointestinal stromal tumor (GIST) is the most common sarcoma and its treatment with imatinib has served as the paradigm for developing targeted anti-cancer therapies. Despite this success, imatinib-resistance has emerged as a major problem and therefore, the clinical efficacy of other drugs has been investigated. Unfortunately, most clinical trials have failed to identify efficacious drugs despite promising in vitro data and pathological responses in subcutaneous xenografts. We hypothesized that it was feasible to develop orthotopic patient-derived xenografts (PDXs) from resected GIST that could recapitulate the genetic heterogeneity and biology of the human disease.MethodsFresh tumor tissue from three patients with pathologically confirmed GISTs was obtained immediately following tumor resection. Tumor fragments (4.2-mm3) were surgically xenografted into the liver, gastric wall, renal capsule, and pancreas of immunodeficient mice. Tumor growth was serially assessed with ultrasonography (US) every 3-4 weeks. Tumors were also evaluated with positron emission tomography (PET). Animals were sacrificed when they became moribund or their tumors reached a threshold size of 2500-mm3. Tumors were subsequently passaged, as well as immunohistochemically and histologically analyzed.ResultsHerein, we describe the first model for generating orthotopic GIST PDXs. We have successfully xenografted three unique KIT-mutated tumors into a total of 25 mice with an overall success rate of 84% (21/25). We serially followed tumor growth with US to describe the natural history of PDX growth. Successful PDXs resulted in 12 primary xenografts in NOD-scid gamma or NOD-scid mice while subsequent successful passages resulted in 9 tumors. At a median of 7.9 weeks (range 2.9-33.1 weeks), tumor size averaged 473 ± 695-mm³ (median 199-mm3, range 12.6-2682.5-mm³) by US. Furthermore, tumor size on US within 14 days of death correlated with gross tumor size on necropsy. We also demonstrated that these tumors are FDG-avid on PET imaging, while immunohistochemically and histologically the PDXs resembled the primary tumors.ConclusionsWe report the first orthotopic model of human GIST using patient-derived tumor tissue. This novel, reproducible in vivo model of human GIST may enhance the study of GIST biology, biomarkers, personalized cancer treatments, and provide a preclinical platform to evaluate new therapeutic agents for GIST

Rapid Mutation of Endogenous Zebrafish Genes Using Zinc Finger Nucleases Made by Oligomerized Pool ENgineering (OPEN)

Background: Customized zinc finger nucleases (ZFNs) form the basis of a broadly applicable tool for highly efficient genome modification. ZFNs are artificial restriction endonucleases consisting of a non-specific nuclease domain fused to a zinc finger array which can be engineered to recognize specific DNA sequences of interest. Recent proof-of-principle experiments have shown that targeted knockout mutations can be efficiently generated in endogenous zebrafish genes via non-homologous end-joining-mediated repair of ZFN-induced DNA double-stranded breaks. The Zinc Finger Consortium, a group of academic laboratories committed to the development of engineered zinc finger technology, recently described the first rapid, highly effective, and publicly available method for engineering zinc finger arrays. The Consortium has previously used this new method (known as OPEN for Oligomerized Pool ENgineering) to generate high quality ZFN pairs that function in human and plant cells. Methodology/Principal Findings: Here we show that OPEN can also be used to generate ZFNs that function efficiently in zebrafish. Using OPEN, we successfully engineered ZFN pairs for five endogenous zebrafish genes: tfr2, dopamine transporter, telomerase, hif1aa, and gridlock. Each of these ZFN pairs induces targeted insertions and deletions with high efficiency at its endogenous gene target in somatic zebrafish cells. In addition, these mutations are transmitted through th