251 research outputs found
The implications of stakeholders' perceptions of land for sustainable land use management in NE Ghana
There are negative implications of changes in stakeholders traditional land perceptions for sustainable land use and management in north-east Ghana. In African tenurial systems, land use was based on a local mystical view of the environment and stakeholders broad-based knowledge of the local environments. These led to sustainable resource use and management. However, in the context of current political ecology of north-east Ghana as induced by increased population growth, urbanisation, the market economy, changes in religious beliefs, and government land policies, stakeholders understandings of land have acquired even greater importance in issues of sustainable land resource use and management. A mixed methodological approach, combining both qualitative and quantitative data gathering techniques for information on stakeholders land perceptions, was used to analyse their implications for sustainable land use and management. Changes in the dynamics of stakeholders perceptions of land are partly responsible for the current state of land and environmental degradation in north-east Ghana. Policies aimed at ensuring sustainable land use and environmental management must focus on those traditional land perceptions, which encourage environmental sustainabilit
Identification of roots from grass swards using PCR-RFLP and FFLP of the plastid trnL (UAA) intron
BACKGROUND: The specific associations between plant roots and the soil microbial community are key to understanding nutrient cycling in grasslands, but grass roots can be difficult to identify using morphology alone. A molecular technique to identify plant species from root DNA would greatly facilitate investigations of the root rhizosphere. RESULTS: We show that trnL PCR product length heterogeneity and a maximum of two restriction digests can separate 14 common grassland species. The RFLP key was used to identify root fragments at least to genus level in a field study of upland grassland community diversity. Roots which could not be matched to known types were putatively identified by comparison of the nuclear ribosomal ITS sequence to the GenBank database. Ten taxa were identified among almost 600 root fragments. Additionally, we have employed capillary electrophoresis of fluorescent trnL PCR products (fluorescent fragment length polymorphism, FFLP) to discriminate all taxa identified at the field site. CONCLUSION: We have developed a molecular database for the identification of some common grassland species based on PCR-RFLP of the plastid transfer RNA leucine (trnL) UAA gene intron. This technique will allow fine-scale studies of the rhizosphere, where root identification by morphology is unrealistic and high throughput is desirable
High-Resolution Infrared Spectroscopy of the Brown Dwarf Epsilon Indi Ba
We report on the analysis of high-resolution infrared spectra of the newly
discovered brown dwarf Epsilon Indi Ba. This is the closest known brown dwarf
to the solar system, with a distance of 3.626 pc. Spectra covering the ranges
of 2.308-2.317 microns and 1.553-1.559 microns were observed at a spectral
resolution of R=50,000 with the Phoenix spectrometer on the Gemini South
telescope. The physical paramters of effective temperature and surface gravity
are derived by comparison to model spectra calculated from atmospheres computed
using unified cloudy models. An accurate projected rotational velocity is also
derived.Comment: 9 pages, 3 figures. Astrophysical Journal Letters, in pres
Interferometry
The following recommended programs are reviewed: (1) infrared and optical interferometry (a ground-based and space programs); (2) compensation for the atmosphere with adaptive optics (a program for development and implementation of adaptive optics); and (3) gravitational waves (high frequency gravitational wave sources (LIGO), low frequency gravitational wave sources (LAGOS), a gravitational wave observatory program, laser gravitational wave observatory in space, and technology development during the 1990's). Prospects for international collaboration and related issues are also discussed
The Science Case for the Planet Formation Imager (PFI)
Among the most fascinating and hotly-debated areas in contemporary
astrophysics are the means by which planetary systems are assembled from the
large rotating disks of gas and dust which attend a stellar birth. Although
important work has already been, and is still being done both in theory and
observation, a full understanding of the physics of planet formation can only
be achieved by opening observational windows able to directly witness the
process in action. The key requirement is then to probe planet-forming systems
at the natural spatial scales over which material is being assembled. By
definition, this is the so-called Hill Sphere which delineates the region of
influence of a gravitating body within its surrounding environment. The Planet
Formation Imager project (PFI) has crystallized around this challenging goal:
to deliver resolved images of Hill-Sphere-sized structures within candidate
planet-hosting disks in the nearest star-forming regions. In this contribution
we outline the primary science case of PFI. For this purpose, we briefly review
our knowledge about the planet-formation process and discuss recent
observational results that have been obtained on the class of transition disks.
Spectro-photometric and multi-wavelength interferometric studies of these
systems revealed the presence of extended gaps and complex density
inhomogeneities that might be triggered by orbiting planets. We present
detailed 3-D radiation-hydrodynamic simulations of disks with single and
multiple embedded planets, from which we compute synthetic images at
near-infrared, mid-infrared, far-infrared, and sub-millimeter wavelengths,
enabling a direct comparison of the signatures that are detectable with PFI and
complementary facilities such as ALMA. From these simulations, we derive some
preliminary specifications that will guide the array design and technology
roadmap of the facility.Comment: SPIE Astronomical Telescopes and Instrumentation conference, June
2014, Paper ID 9146-120, 13 pages, 3 Figure
Effects of polygenic risk for suicide attempt and risky behavior on brain structure in young people with familial risk of bipolar disorder
Bipolar disorder (BD) is associated with a 20–30-fold increased suicide risk compared to the general population. First-degree relatives of BD patients show inflated rates of psychopathology including suicidal behaviors. As reliable biomarkers of suicide attempts (SA) are lacking, we examined associations between suicide-related polygenic risk scores (PRSs)—a quantitative index of genomic risk—and variability in brain structures implicated in SA. Participants (n = 206; aged 12–30 years) were unrelated individuals of European ancestry and comprised three groups: 41 BD cases, 96 BD relatives (“high risk”), and 69 controls. Genotyping employed PsychArray, followed by imputation. Three PRSs were computed using genome-wide association data for SA in BD (SA-in-BD), SA in major depressive disorder (SA-in-MDD) (Mullins et al., 2019, The American Journal of Psychiatry, 176(8), 651–660), and risky behavior (Karlsson Linnér et al., 2019, Nature Genetics, 51(2), 245–257). Structural magnetic resonance imaging processing employed FreeSurfer v5.3.0. General linear models were constructed using 32 regions-of-interest identified from suicide neuroimaging literature, with false-discovery-rate correction. SA-in-MDD and SA-in-BD PRSs negatively predicted parahippocampal thickness, with the latter association modified by group membership. SA-in-BD and Risky Behavior PRSs inversely predicted rostral and caudal anterior cingulate structure, respectively, with the latter effect driven by the “high risk” group. SA-in-MDD and SA-in-BD PRSs positively predicted cuneus structure, irrespective of group. This study demonstrated associations between PRSs for suicide-related phenotypes and structural variability in brain regions implicated in SA. Future exploration of extended PRSs, in conjunction with a range of biological, phenotypic, environmental, and experiential data in high risk populations, may inform predictive models for suicidal behaviors.Australian National Health and Medical Research Council (NHMRC); Lansdowne Foundation, Paul and Jenny Reid, Good Talk, and the Keith Pettigrew Family Bequest. DNA extraction was undertaken by Genetic Repositories Australia (GRA; www.neura.edu.au/GRA), Claudio Toma is a recipient of a “Ramón y Cajal” fellowship (RYC2018-024106-I) from the Spanish MINECO. This research was undertaken with the assistance of resources from the National Computational Infrastructure (NCI), which is supported by the Australian Governmen
IMOS national reference stations: A continental-wide physical, chemical and biological coastal observing system
Sustained observations allow for the tracking of change in oceanography and ecosystems, however, these are rare, particularly for the Southern Hemisphere. To address this in part, the Australian Integrated Marine Observing System (IMOS) implemented a network of nine National Reference Stations (NRS). The network builds on one long-term location, where monthly water sampling has been sustained since the 1940s and two others that commenced in the 1950s. In-situ continuously moored sensors and an enhanced monthly water sampling regime now collect more than 50 data streams. Building on sampling for temperature, salinity and nutrients, the network now observes dissolved oxygen, carbon, turbidity, currents, chlorophyll a and both phytoplankton and zooplankton. Additional parameters for studies of ocean acidification and bio-optics are collected at a sub-set of sites and all data is made freely and publically available. Our preliminary results demonstrate increased utility to observe extreme events, such as marine heat waves and coastal flooding; rare events, such as plankton blooms; and have, for the first time, allowed for consistent continental scale sampling and analysis of coastal zooplankton and phytoplankton communities. Independent water sampling allows for cross validation of the deployed sensors for quality control of data that now continuously tracks daily, seasonal and annual variation. The NRS will provide multi-decadal time series, against which more spatially replicated short-term studies can be referenced, models and remote sensing products validated, and improvements made to our understanding of how large-scale, long-term change and variability in the global ocean are affecting Australia's coastal seas and ecosystems. The NRS network provides an example of how a continental scaled observing systems can be developed to collect observations that integrate across physics, chemistry and biology
Intravital FRAP imaging using an E-cadherin-GFP mouse reveals disease- and drug-dependent dynamic regulation of cell-cell junctions in live tissue
E-cadherin-mediated cell-cell junctions play a prominent role in maintaining the epithelial architecture. The disruption or deregulation of these adhesions in cancer can lead to the collapse of tumor epithelia that precedes invasion and subsequent metastasis. Here we generated an E-cadherin-GFP mouse that enables intravital photobleaching and
quantification of E-cadherin mobility in live tissue without affecting normal biology. We demonstrate the broad applications of this mouse by examining
E-cadherin regulation in multiple tissues, including mammary, brain, liver, and kidney tissue, while specifically monitoring E-cadherin mobility during
disease progression in the pancreas. We assess E-cadherin stability in native pancreatic tissue upon genetic manipulation involving Kras and p53
or in response to anti-invasive drug treatment and gain insights into the dynamic remodeling of E-cadherin during in situ cancer progression. FRAP in the E-cadherin-GFP mouse, therefore, promises to be a valuable tool to fundamentally expand our understanding of E-cadherin-mediated events in native microenvironments
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