In situ benthic fluxes from an intermittently active mud volcano at the Costa Rica convergent margin

Along the erosive convergent margin off Costa Rica a large number of mound-shaped structures exist built by mud diapirism or mud volcanism. One of these, Mound 12, an intermittently active mud volcano, currently emits large amounts of aqueous dissolved species and water. Chemosynthetic vent communities, authigenic carbonates, and methane plumes in the water column are manifestations of that activity. Benthic flux measurements were obtained by a video-guided Benthic Chamber Lander (BCL) deployed at a vent site located in the most active part of Mound 12. The lander was equipped with 4 independent chambers covering adjacent areas of the seafloor. Benthic fluxes were recorded by repeated sampling of the enclosed bottom waters while the underlying surface sediments were recovered with the lander after a deployment time of one day. One of the chambers was placed directly in the centre of an active vent marked by the occurrence of a bacterial mat while the other chambers were located at the fringe of the same vent system at a lateral distance of only 40 cm. A transport-reaction model was developed and applied to describe the concentration profiles in the pore water of the recovered surface sediments and the temporal evolution of the enclosed bottom water. Repeated model runs revealed that the best fit to the pore water and benthic chamber data is obtained with a flow velocity of 10 cm yr− 1 at the centre of the vent. The flux rates to the bottom water are strongly modified by the benthic turnover (benthic filter). The methane flux from below at the bacterial mat site is as high as 1032 μmol cm− 2 yr− 1, out of which 588 μmol cm− 2 yr− 1 is oxidised in the surface sediments by microbial consortia using sulphate as terminal electron acceptor and 440 μmol cm− 2 yr− 1 are seeping into the overlaying bottom water. Sulphide is transported to the surface by ascending fluids (238 μmol cm− 2 yr− 1) and is formed within the surface sediment by the anaerobic oxidation of methane (AOM, 588 μmol cm− 2 yr− 1). However, sulphide is not released into the bottom water but completely oxidized by oxygen and nitrate at the sediment/water interface. The oxygen and nitrate fluxes into the sediment are high (781 and 700 μmol cm− 2 yr− 1, respectively) and are mainly driven by the microbial oxidation of sulphide. Benthic fluxes were much lower in the other chambers placed in the fringe of the vent system. Thus, methane and oxygen fluxes of only 28 and 89 μmol cm− 2 yr− 1, respectively were recorded in one of these chambers. Our study shows that the aerobic oxidation of methane is much less efficient than the anaerobic oxidation of methane so that methane which is not oxidized within the sediment by AOM is almost completely released into the bottom water. Hence, anaerobic rather than aerobic methane oxidation plays the major role in the regulation of benthic methane fluxes. Moreover, we demonstrate that methane and oxygen fluxes at cold vent sites may vary up to 3 orders of magnitude over a lateral distance of only 40 cm indicating an extreme focussing of fluid flow and methane release at the seafloor

Superoxide release and cellular gluthatione peroxidase activity in leukocytes from children with persistent asthma

Asthma is an inflammatory condition characterized by the involvement of several mediators, including reactive oxygen species. The aim of the present study was to investigate the superoxide release and cellular glutathione peroxidase (cGPx) activity in peripheral blood granulocytes and monocytes from children and adolescents with atopic asthma. Forty-four patients were selected and classified as having intermittent or persistent asthma (mild, moderate or severe). The spontaneous or phorbol myristate acetate (PMA, 30 nM)-induced superoxide release by granulocytes and monocytes was determined at 0, 5, 15, and 25 min. cGPx activity was assayed spectrophotometrically. The spontaneous superoxide release by granulocytes from patients with mild (N = 15), moderate (N = 12) or severe (N = 6) asthma was higher at 25 min compared to healthy individuals (N = 28, P andlt; 0.05, Duncan test). The PMA-induced superoxide release by granulocytes from patients with moderate (N = 12) or severe (N = 6) asthma was higher at 15 and 25 min compared to healthy individuals (N = 28, P andlt; 0.05 in both times of incubation, Duncan test). The spontaneous or PMA-induced superoxide release by monocytes from asthmatic patients was similar to healthy individuals (P \u3e 0.05 in all times of incubation, Duncan test). cGPx activity of granulocytes and monocytes from patients with persistent asthma (N = 20) was also similar to healthy individuals (N = 10, P \u3e 0.05, Kruskal-Wallis test). We conclude that, under specific circumstances, granulocytes from children with persistent asthma present a higher respiratory burst activity compared to healthy individuals. These findings indicate a risk of oxidative stress, phagocyte auto-oxidation, and the subsequent release of intracellular toxic oxidants and enzymes, leading to additional inflammation and lung damage in asthmatic children

Person-centered approaches to examining links between self-regulation and conduct problems, attention-deficit/hyperactivity disorder, and callous-unemotional behaviors in childhood

Over the past two decades, the study of self-regulation and its associations with emerging psychopathology has become a major pursuit in developmental science. Early-childhood emotion regulation (ER) and executive function (EF), in particular, are interrelated aspects of self-regulation that have garnered extensive research and are theorized to promote social competence school readiness and achievement, and adjustment. However, the development of self-regulation is a complex process that occurs through coaction at multiple levels of analysis. Three studies were conducted to examine biobehavioral emotion responding in infancy, early childhood EF, and their prospective influences on trajectories of conduct problems (CPs), attention-deficit/hyperactivity disorder (ADHD), and callous-unemotional (CU) behaviors using multiple person-centered approaches. Study 1 used latent profile analysis (LPA) to prospectively examine the synchrony and asynchrony of infant behavioral reactivity, cortisol reactivity, and ER behaviors at 6, 15, and 24 months of age to determine whether groups of infants evidenced different patterns of arousal and regulation; and whether such patterns were bidirectionally related to parenting behavior over the same span of time. Study 2 used longitudinal latent class analysis (LLCA) to examine joint trajectories of CPs, ADHD symptoms, and CU behaviors from 3 years old to 5th grade in order to assess examine heterogeneity in CPs based on the presence of ADHD and CU behaviors. Study 3 built upon the prior two studies by in by investigating associations of infants’ emotional arousal and regulation with their later CP/ADUD/CU trajectories, as well as the role of early childhood EF in mediating these prospective associations. Results from Study 1 indicated that there is observable variation in infants’ patterns of behavioral reactivity, cortisol reactivity, and ER behaviors across infancy, and that infant emotion responding and parent sensitivity and harsh-intrusion were bidirectionally predictive of one another. Results from Study 2 showed that children did follow differing trajectories of CPs, but that these varied based on who reported their behavior (parents, teachers, or both), rather than on trajectories of ADHD symptoms and CU behaviors. In addition, these joint trajectories differentiated children’s likelihood of meeting diagnostic criteria for oppositional defiant disorder, conduct disorder, and ADHD, as well as clinically significant levels of CU behaviors, during preadolescence. Finally, results from Study 3 indicated that infants’ patterns of emotion responding were not prospectively related to their CP/ADHD/CU trajectories or their early childhood EF. However, better EF did significantly predict a decreased likelihood of following trajectories characterized by high problem behavior as rated by both parents and teachers, parents only, and teachers only. The implications for understanding the early development of self-regulation, CPs, ADHD, and CU behaviors are discussed, as is the utility of innovative person-centered approaches for understanding these phenomena

Emotion recognition deficits among children with conduct problems and callous-unemotional behaviors: differences by child race

Deficits in emotion recognition have been associated with psychopathic and callous-unemotional (CU) behaviors among adults, adolescents, and children. However, few previous studies have examined such associations exclusively during early and middle childhood. The current study used a large, population-stratified, randomly-selected sample of 2nd grade children living in areas of high rural poverty to examine group differences in emotion recognition among children showing no conduct problems or CU behaviors (typical), conduct problems without CU behaviors (CP-only), and both CP and CU behaviors (CP+CU). Primary caregivers reported on children’s conduct problems and callous-unemotional behaviors at 1st grade and children completed a computerized facial emotion recognition task at 2nd grade. Results indicated that group differences in emotion recognition accuracy were moderated by child race, with children in the typical group showing better overall accuracy and better recognition of fearful and happy faces among European American children, whereas no group differences were found among African American children. Implications for emotion socialization, etiology of CP and CU behaviors, and future directions for research and treatment are discussed

Respiratory sinus arrhythmia and heart period in infancy as correlates of later oppositional defiant and callous-unemotional behaviors

Extant literature suggests that oppositional defiant (ODD) and callous-unemotional (CU) behaviors in childhood and adolescence are associated with distinct patterns of psychophysiological functioning and that individual differences in these patterns have implications for developmental pathways to disorder. Very little is known about the associations between psychophysiological functioning in infancy and later ODD and CU behaviors. This study examined associations between basal autonomic nervous system (ANS) functioning in infancy and ODD and CU behaviors in later childhood. Using longitudinal heart period (HP) and respiratory sinus arrhythmia (RSA) data from the Durham Child Health and Development Study (N = 206), the current study tested associations, within a structural equation modeling framework, between continuous measures of HP and RSA across the first two years of life and later ODD and CU behaviors at first grade. Results indicate that ODD and CU behaviors in childhood are associated with lower baseline RSA, but not HP, across infancy. The implications of these findings for developmental models of ODD and CU behaviors are discussed

Enhanced lifetime of methane bubble streams within the deep ocean

We have made direct comparisons of the dissolution and rise rates of methane and argon bubbles experimentally released in the ocean at depths from 440 to 830 m. The bubbles were injected from the ROV Ventana into a box open at the top and the bottom, and imaged by HDTV while in free motion. The vehicle was piloted upwards at the rise rate of the bubbles. Methane and argon show closely similar behavior at depths above the methane hydrate stability field. Below that boundary (∼520 m) markedly enhanced methane bubble lifetimes are observed, and are attributed to the formation of a hydrate skin. This effect greatly increases the ease with which methane gas released at depth, either by natural or industrial events, can penetrate the shallow ocean layers

The effect of IFN-gamma and TNF-alpha on the NADPH oxidase system of human colostrum macrophages, blood monocytes, and THP-1 cells

The aim of this work was to analyze the effect of Interferon-gamma (IFN-gamma) and tumor necrosis factor-alpha (TNF-alpha) on NADPH oxidase activity and gp91-phox gene expression in human colostrum macrophages (CM), peripheral blood monocytes (PBM), and myelomonocytic THP-1 cells. We also investigated the effect of IFN-gamma on the release of TNF-alpha by these cells. Our results show that under basal culture conditions, CM release more superoxide than PBM and THP-1 cells (p andlt; 0.05). The addition of IFN-gamma, alone or in combination with TNF-alpha, increased spontaneous superoxide release by PBM and THP-1 cells (p andlt; 0.05) and increased phorbol myristate acetate (PMA)-stimulated superoxide release by CM, PBM, and THP-1 cells (p andlt; 0.05). The NADPH oxidase activity of THP-1 cells consistently remained lower than that of CM or PBM, despite a dramatic response to IFN-gamma and TNF-alpha. Under basal conditions, gp91-phox gene expression was significantly higher in CM and PBM compared with THP-1 cells (p andlt; 0.05). The addition of IFN-gamma alone or in combination with TNF-alpha caused a dramatic increase in gp91-phox gene expression in THP-1 cells (p andlt; 0.05) but not in CM or PBM. Under basal conditions or in the presence of IFN-gamma, CM released more TNF-alpha than PBM or THP-1 cells (p andlt; 0.05). In addition, PBM released more TNF-gamma than THP-1 cells (p andlt; 0.05). IFN-gamma did not significantly augment the release of TNF-alpha by these cells (p \u3e 0.05). Thus, IFN-gamma and TNF-alpha induced equivalent gp91-phox gene expression in THP-1 cells compared with CM or PBM but did not bring about equivalent NADPH oxidase activity. TNF-alpha release was higher in more mature cells. This partial divergence of gp91- phox gene expression, NADPH oxidase activity, and TNF-alpha release is probably a consequence of different events of myeloid cell biology and relates at least in part to cell differentiation state

Supernumerary kidneys: a clinical and radiological analysis of nine cases.

BACKGROUND: A supernumerary kidney (SK) is an additional kidney with its own capsule and blood supply that is not fused with the ipsilateral kidney (IK). Because individual case reports indicate a high morbidity rate, the aim of this retrospective study was a detailed analysis of this rare anatomical variant. METHODS: Our systematic imaging-based search for SKs, conducted in the period from 2000 and to 2017, yielded 9 cases in total (5 men, 4 women; mean age: 51.8 ± 22.8 years). RESULTS: The SKs were observed on the right in six and on the left side in three cases. In six subjects (66%) they were located caudal and in three cases (33%) cranial to the ipsilateral kidney. Calculi were found in three (33%) of the renal collecting systems. Five (56%) of the SKs had hydronephrosis grade IV and one SK had recurrent pyelonephritis (11%). Two of the ureters opened into the ipsilateral seminal vesicle (22%). Two (22%) SKs were functional but atrophic. Clinically relevant findings were made in 33% of the IKs: atrophy (n = 2), calculi (n = 1), and reflux with recurrent pyelonephritis (n = 1); another 33% had anatomical anomalies without functional impairment. The correct diagnosis of a SK is possible using CT imaging in all subjects. The prevalence of SK based on CT imaging can be estimated to be 1:26750. CONCLUSIONS: CT is the method of choice for visualizing SKs. The correct diagnosis is crucial in preventing dispensable surgical procedures and for providing optimal patient treatment and outcome

Improvement of quality of life, anxiety and depression after surgery in patients with stress urinary incontinence: Results of a longitudinal short-term follow-up

<p>Abstract</p> <p>Objective</p> <p>The objective of this study was to compare the effect of incontinence surgery and pelvic floor training on quality of life (QOL), anxiety and depression in patients with stress urinary incontinence (SUI).</p> <p>Methods</p> <p>In a prospective longitudinal study, females with proven SUI were asked to complete a set of standardized questionnaires (sociodemographic data sheet, FACT-G, I-QOL, HADS) before and eight weeks after treatment. The comparison groups consisted of a surgical treatment group and a conservative group that underwent supervised pelvic floor training for eight weeks.</p> <p>Results</p> <p>From the 67 female patients included in the study a number of 53 patients completed both assessment time points (mean age 57.4, mean years of SUI 7.6). The surgical treatment group consisted of 32 patients of which 21 patients received a modified Burch colposuspension and 11 patients a tension-free mid-urethral tape suspension. The 21 patients in the conservative group attended eight once-weekly supervised pelvic floor training sessions.</p> <p>After treatment the surgical intervention group showed a significantly higher improvement of QOL (FACT-G and I-QOL) and anxiety (HADS) than the pelvic floor training group.</p> <p>Conclusion</p> <p>For female patients with SUI surgery yielded a better outcome than pelvic floor training with regard to quality of life and anxiety.</p