2,498 research outputs found
Transcriptional regulation of prostate kallikrein-like genes by androgen.
Using gene-specific synthetic oligonucleotides the
expression and regulation of kallikrein-like genes in
the human prostatic cancer cell line LNCaP were
studied. Prostate-specific antigen (PSA) and human
glandular kallikrein (hGK-1) together constitute a
subfamily of serine proteases exclusively produced
in the human prostate. RNA analysis revealed that
both genes are expressed in LNCaP cells with PSA
basal levels being 2-fold higher than hGK-1 levels.
Both mRNAs are induced over a period of 24 h in
the presence of 3.3 nM of the synthetic androgen
mibolerone. Stimulation of PSA RNA is about 5-
fold,whereas hGK-1 stimulation is less pronounced.
Nuclear run-on analysis revealed that androgen induction
of kallikrein-like genes in LNCaP cells is a
rapid event (c3 h) occurring at the level of transcription
initiation. Treatment of cells with cycloheximide
demonstrates that, while PSA/hGK-1 basal transcription
strictly depends on continuous protein synthesis,
transcriptional induction by androgen does
not. This suggests the direct involvement of the
androgen receptor in the induction process independent
of additional labile protein factors necessary
for kallikrein basal transcription. A binding motif
is present in the PSA and hGK-1 promoters, closely
resembling the consensus sequence for steroidresponsive
elements. The androgen antagonist cyproterone
acetate was also able to stimulate transcription
of kallikrein-like genes in LNCaP cells. In
contrast, androgen-dependent transcriptional
suppression of the protooncogene c-myc was
strongly counteracted by cyproterone acetate. Thus,
antiandrogens act differentially on androgen-regulated
prostate-specific (PSA, hGK-1) and growthrelated
(c-myc) gene expression in LNCaP cells
Treating critically ill patients with probiotics: Beneficial or dangerous?
Probiotic bacteria are live microorganisms which confer to health benefits of the host. They help to maintain the integrity of the intestinal barrier function by modulating the mucosal and systemic immune response of the host. These bacteria have proven their beneficial effect in several conditions of ulcerative colitis. More recently probiotics/synbiotics have been included in the treatment of critically ill patients. However to date it remains uncertain whether probiotics/synbiotics are beneficial or even dangerous to the clinical outcome of this patient group. This article reviews the current evidence of the use of bacteria in critically ill patients in intensive care settings
Nanomateriais e a interface entre nanotecnologia e ambiente
Nanomaterials are the main products of nanotechnology. In this paper we describe some of these nanomaterials, particularly Carbon based systems, their properties, manipulation strategies and applications. History of nanoparticles, as well as the complexity in defining nanomaterials, necessary for regulation and assessment of impacts on society, are also addressed.Nanomateriais são os principais produtos das nanotecnologias. Nesse artigo são descritos alguns desses nanomateriais, em particular os compostos de Carbono, suas propriedades, estratégias de manipulação e aplicações. Discute-se também a história das nanopartículas e a complexidade na classificação desses materiais, necessária para sua regulamentação e avaliação de impactos na sociedade
Linguagens Perdidas De Divulgação Científica: Simulacros E Artesanatos
Esse artigo propõe um resgate da história de uma prática que foi paulatinamente sendo suplantada pelas novas tecnologias de linguagem em divulgação científica. É um manifesto em forma de crônica, um registro, para propor uma reavaliação dos kits de experimentação científica, que alcançaram talvez o auge de sua popularidade nos anos 70 do século passado.15248-56Estudos Urbano
Two clouds still obscure the reputation of Lord Kelvin
Lord Kelvin is considered one of the most important physicists of the nineteenth century. With multiple talents and a public man, he is the author of famous aphorisms, but many phrases attributed to him are not more than urban legends. One of the most known is that there are only two little clouds in the sky of physics and many variations, alluding the belief of completeness in physics at the beginning of the last century. The two little clouds are nothing else than the theory of relativity and quantum mechanics, the two pillars of modern physics. In opposition to what is often propagated, Lord Kelvin seemed to be quite aware of the dead end of classical physics at that time and gave a clear indication to the newcomers of the paths to be followed.Lorde Kelvin é considerado um dos mais importantes físicos do século XIX. Talento multifacetado e homem público é autor de aforismos famosos, mas muitas frases a ele atribuídas não passam de lendas urbanas. Uma das mais famosas é a de existem apenas duas nuvenzinhas no céu da física, e suas variantes, aludindo à crença de completude da física no início do século passado. As duas nuvenzinhas nada mais são do que a teoria da relatividade e a mecânica quântica, os dois pilares da física moderna. Ao contrário do que muitos propagam, Lorde Kelvin na verdade parecia estar bem ciente do impasse da física clássica na época e deixou para os seus sucessores uma indicação clara de que rumos deveriam ser seguidos.50951
Lord of the Rings: A Kinematic Distance to Circinus X-1 from a Giant X-Ray Light Echo
Circinus X-1 exhibited a bright X-ray flare in late 2013. Follow-up
observations with Chandra and XMM-Newton from 40 to 80 days after the flare
reveal a bright X-ray light echo in the form of four well-defined rings with
radii from 5 to 13 arcminutes, growing in radius with time. The large fluence
of the flare and the large column density of interstellar dust towards Circinus
X-1 make this the largest and brightest set of rings from an X-ray light echo
observed to date. By deconvolving the radial intensity profile of the echo with
the MAXI X-ray lightcurve of the flare we reconstruct the dust distribution
towards Circinus X-1 into four distinct dust concentrations. By comparing the
peak in scattering intensity with the peak intensity in CO maps of molecular
clouds from the Mopra Southern Galactic Plane CO Survey we identify the two
innermost rings with clouds at radial velocity ~ -74 km/s and ~ -81 km/s,
respectively. We identify a prominent band of foreground photoelectric
absorption with a lane of CO gas at ~ -32 km/s. From the association of the
rings with individual CO clouds we determine the kinematic distance to Circinus
X-1 to be kpc. This distance rules out
earlier claims of a distance around 4 kpc, implies that Circinus X-1 is a
frequent super-Eddington source, and places a lower limit of on the Lorentz factor and an upper limit of on the jet viewing angle.Comment: 20 pages, 21 figures, Astrophysical Journal, in prin
Robust signatures in the current-voltage characteristics of DNA molecules oriented between two graphene nanoribbon electrodes
In this work we numerically calculate the electric current through three
kinds of DNA sequences (telomeric, \lambda-DNA, and p53-DNA) described by
different heuristic models. A bias voltage is applied between two zig-zag edged
graphene contacts attached to the DNA segments, while a gate terminal modulates
the conductance of the molecule. The calculation of current is performed by
integrating the transmission function (calculated using the lattice Green's
function) over the range of energies allowed by the chemical potentials. We
show that a telomeric DNA sequence, when treated as a quantum wire in the fully
coherent low-temperature regime, works as an excellent semiconductor. Clear
steps are apparent in the current-voltage curves of telomeric sequences and are
present independent of lengths and sequence initialisation at the contacts. The
current-voltage curves suggest the existence of stepped structures independent
of length and sequencing initialisation at the contacts. We also find that the
molecule-electrode coupling can drastically influence the magnitude of the
current. The difference between telomeric DNA and other DNA, such as
\lambda-DNA and DNA for the tumour suppressor p53, is particularly visible in
the length dependence of the current
An Exactly Solvable Model of N Coupled Luttinger Chains
We calculate the exact Green function of a special model of N coupled
Luttinger chains with arbitrary interchain hopping t_{perp}. The model is
exactly solvable via bosonization if the interchain interaction does not fall
off in the direction perpendicular to the chains. For any finite N we find
Luttinger liquid behavior and explicitly calculate the anomalous dimension
gamma^(N). However, the Luttinger liquid state does not preclude coherent
interchain hopping. We also show that gamma^(N) -> 0 for N -> infinity, so that
in the limit of infinitely many chains we obtain a Fermi liquid.Comment: accepted for publication in Phys. Rev.
Identification of high-wind features within extratropical cyclones using a probabilistic random forest – Part 1: Method and case studies
Visible-to-telecom quantum frequency conversion of light from a single quantum emitter
Quantum frequency conversion (QFC), a nonlinear optical process in which the
frequency of a quantum light field is altered while conserving its
non-classical correlations, was first demonstrated 20 years ago. Meanwhile, it
is considered an essential tool for the implementation of quantum repeaters
since it allows for interfacing quantum memories with telecom-wavelength
photons as quantum information carriers. Here we demonstrate efficient (>30%)
QFC of visible single photons (711 nm) emitted by a quantum dot (QD) to a
telecom wavelength (1,313 nm). Analysis of the first and second-order coherence
before and after wavelength conversion clearly proves that important
properties, such as the coherence time and photon antibunching, are fully
conserved during the frequency translation process. Our findings underline the
great potential of single photon sources on demand in combination with QFC as a
promising technique for quantum repeater schemes.Comment: 11 pages, 4 figure
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