Life cycle and host range of Phycitasp. rejected for biological control of prickly acacia in Australia

Prickly acacia (Vachellia nilotica subsp. indica), a native of the Indian subcontinent, is a serious weed of the grazing areas of northern Australia and is a target for classical biological control. Native range surveys in India identified a leaf webber, Phycita sp. (Lepidoptera: Pyralidae) as a prospective biological control agent for prickly acacia. In this study, we report the life cycle and host-specificity test results Phycita sp. and highlight the contradictory results between the no-choice tests in India and Australia and the field host range in India. In no-choice tests in India and Australia, Phycita sp. completed development on two of 11 and 16 of 27 non-target test plant species, respectively. Although Phycita sp. fed and completed development on two non-target test plant species (Vachellia planifrons and V. leucophloea) in no-choice tests in India, there was no evidence of the insect on the two non-target test plant species in the field. Our contention is that oviposition behaviour could be the key mechanism in host selection of Phycita sp., resulting in its incidence only on prickly acacia in India. This is supported by paired oviposition choice tests involving three test plant species (Acacia baileyana, A. mearnsii and A. deanei) in quarantine in Australia, where eggs were laid only on prickly acacia. However, in paired oviposition choice trials, only few eggs were laid, making the results unreliable. Although oviposition choice tests suggest that prickly acacia is the most preferred and natural host, difficulties in conducting choice oviposition tests with fully grown trees under quarantine conditions in Australia and the logistic difficulties of conducting open-field tests with fully grown native Australian plants in India have led to rejection of Phycita sp. as a potential biological control agent for prickly acacia in Australia

Time-dependent reduction of structural complexity of the buccal epithelial cell nuclei after treatment with silver nanoparticles

Recent studies have suggested that silver nanoparticles (AgNPs) may affect cell DNA structure in in vitro conditions. In this paper, we present the results indicating that AgNPs change nuclear complexity properties in isolated human epithelial buccal cells in a time-dependent manner. Epithelial buccal cells were plated in special tissue culture chamber / slides and were kept at 37°C in an RPMI 1640 cell culture medium supplemented with L-glutamine. The cells were treated with colloidal silver nanoparticles suspended in RPMI 1640 medium at the concentration 15 mg L−1. Digital micrographs of the cell nuclei in a sample of 30 cells were created at five different time steps: before the treatment (controls), immediately after the treatment, as well as 15 , 30 and 60 min after the treatment with AgNPs. For each nuclear structure, values of fractal dimension, lacunarity, circularity, as well as parameters of grey level co-occurrence matrix (GLCM) texture, were determined. The results indicate time-dependent reduction of structural complexity in the cell nuclei after the contact with AgNPs. These findings further suggest that AgNPs, at concentrations present in today's over-the-counter drug products, might have significant effects on the cell genetic material

Kartierung der Zwergrostresistenz der Gerste MBR1012

Zusammenfassung Der Zwergrost gehört zu den wichtigsten Krankheitserregern der Gerste und kann im gemäßigten Klima Ertragsverluste von über 60 % verursachen. Auf der Suche nach neuen Resistenzquellen wurde in der aus Serbien-Montenegro stammenden Landrasse MBR1012 eine wirksame Resistenz gegenüber Zwergrost identifiziert. Um die Vererbung der Resistenz dieser Landrasse aufzuklären, wurden 76 DH-Linien aus der Kreuzung der resistenten Landrasse MBR1012 mit der anfälligen Sorte Scarlett mit der hochvirulenten Zwergrostrasse I80 künstlich inokuliert. Die Spaltung der DH-Population nach der Inokulation mit I80 zeigte, dass die Resistenz monogenetisch vererbt wird. Basierend auf den Ergebnissen der bulked segregant analysis konnte die Resistenz dem Chromosom 1H zugeordnet werden und eng gekoppelte molekulare Marker entwickelt werden, welche eine beschleunigte Übertragung dieser Resistenz in adaptierte Sorten erlauben. Die Suche nach neuen Resistenzen gegen Puccinia hordei ist von besonderer Bedeutung, da die Rostpilze durch Mutation und Rekombination zahlreiche physiologische Rassen bilden, wodurch Resistenzen relativ schnell überwunden werden können. Stichwörter: Gerste (Hordeum vulgare L.), Zwergrost (Puccinia hordei Otth), Resistenz, bulked segregant analysis (BSA), DH-Linien (doubled-haploid).   Abstract Leaf rust (Puccinia hordei Otth) is an economically important disease of barley in temperate regions, causing considerable yield losses up to about 60 %. Resistance to leaf rust has been detected in a landrace derived from Serbia Montenegro (MBR1012). To obtain information on the genetics of resistance of MBR1012 to leaf rust, 76 DH-lines derived from a cross of MBR1012 to the susceptible cv. Scarlett were inoculated using the highly virulent leaf rust isolate I80. It turned out that resistance is inherited in a monogenic manner and by bulked segregant analysis resistance was assigned to chromosome 1H. Next closely linked molecular markers were developed facilitating an efficient introgression into adapted cultivars. Broadening the genetic base of resistance to leaf rust is of special importance as this pathogen is able to overcome resistances quickly due to mutation and recombination. Keywords: Barley (Hordeum vulgare L.), leaf rust (Puccinia hordei Otth), resistance, bulked segregant analysis (BSA), DH-lines (doubled-haploid

Heterostructure Engineering of a Reverse Water Gas Shift Photocatalyst

To achieve substantial reductions in CO2 emissions, catalysts for the photoreduction of CO2 into value‐added chemicals and fuels will most likely be at the heart of key renewable‐energy technologies. Despite tremendous efforts, developing highly active and selective CO2 reduction photocatalysts remains a great challenge. Herein, a metal oxide heterostructure engineering strategy that enables the gas‐phase, photocatalytic, heterogeneous hydrogenation of CO2 to CO with high performance metrics (i.e., the conversion rate of CO2 to CO reached as high as 1400 µmol g cat−1 h−1) is reported. The catalyst is comprised of indium oxide nanocrystals, In2O3−x(OH)y, nucleated and grown on the surface of niobium pentoxide (Nb2O5) nanorods. The heterostructure between In2O3−x(OH)y nanocrystals and the Nb2O5 nanorod support increases the concentration of oxygen vacancies and prolongs excited state (electron and hole) lifetimes. Together, these effects result in a dramatically improved photocatalytic performance compared to the isolated In2O3−x(OH)y material. The defect optimized heterostructure exhibits a 44‐fold higher conversion rate than pristine In2O3−x(OH)y. It also exhibits selective conversion of CO2 to CO as well as long‐term operational stability

Determination of Optimal Locations and Parameters of Passive Harmonic Filters in Unbalanced Systems Using the Multiobjective Genetic Algorithm

This paper discusses the problem of optimal placement and sizing of passive harmonic filters to mitigate harmonics in unbalanced distribution systems. The problem is formulated as a nonlinear multiobjective optimisation problem and solved using the multiobjective genetic algorithm. The performance of the proposed algorithm is tested on unbalanced IEEE 13- and 37-bus three-phase systems. The optimal solutions are obtained based on the following objective functions: 1) minimisation of total harmonic distortion in voltage, 2) minimisation of costs of filters, 3) minimisation of voltage unbalances, and 4) a simultaneous minimisation of total harmonic distortion in voltage, costs of filters, and voltage unbalances. Finally, an analysis of the influence of uncertainties of load powers and changes in system frequency and filter parameters on filter efficiency was performed

Constraints on the χ_(c1) versus χ_(c2) polarizations in proton-proton collisions at √s = 8 TeV

The polarizations of promptly produced χ_(c1) and χ_(c2) mesons are studied using data collected by the CMS experiment at the LHC, in proton-proton collisions at √s=8  TeV. The χ_c states are reconstructed via their radiative decays χ_c → J/ψγ, with the photons being measured through conversions to e⁺e⁻, which allows the two states to be well resolved. The polarizations are measured in the helicity frame, through the analysis of the χ_(c2) to χ_(c1) yield ratio as a function of the polar or azimuthal angle of the positive muon emitted in the J/ψ → μ⁺μ⁻ decay, in three bins of J/ψ transverse momentum. While no differences are seen between the two states in terms of azimuthal decay angle distributions, they are observed to have significantly different polar anisotropies. The measurement favors a scenario where at least one of the two states is strongly polarized along the helicity quantization axis, in agreement with nonrelativistic quantum chromodynamics predictions. This is the first measurement of significantly polarized quarkonia produced at high transverse momentum

Education can improve the negative perception of a threatened long-lived scavenging bird, the Andean condor

Human-wildlife conflicts currently represent one of the main conservation problems for wildlife species around the world. Vultures have serious conservation concerns, many of which are related to people's adverse perception about them due to the belief that they prey on livestock. Our aim was to assess local perception and the factors influencing people's perception of the largest scavenging bird in South America, the Andean condor. For this, we interviewed 112 people from Valle Fértil, San Juan province, a rural area of central west Argentina. Overall, people in the area mostly have an elementary education, and their most important activity is livestock rearing. The results showed that, in general, most people perceive the Andean condor as an injurious species and, in fact, some people recognize that they still kill condors. We identified two major factors that affect this perception, the education level of villagers and their relationship with livestock ranching. Our study suggests that conservation of condors and other similar scavengers depends on education programs designed to change the negative perception people have about them. Such programs should be particularly focused on ranchers since they are the ones who have the worst perception of these scavengers. We suggest that highlighting the central ecological role of scavengers and recovering their cultural value would be fundamental to reverse their persecution and their negative perception by people.Fil: Cailly Arnulphi, Verónica Beatríz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan. Centro de Investigaciones de la Geosfera y Biosfera. Universidad Nacional de San Juan. Facultad de Ciencias Exactas Físicas y Naturales. Centro de Investigaciones de la Geosfera y Biosfera; ArgentinaFil: Lambertucci, Sergio Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; ArgentinaFil: Borghi, Carlos Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan. Centro de Investigaciones de la Geosfera y Biosfera. Universidad Nacional de San Juan. Facultad de Ciencias Exactas Físicas y Naturales. Centro de Investigaciones de la Geosfera y Biosfera; Argentin