Bacterial adhesion on biomedical surfaces covered by yttria stabilized zirconia

The aim of this study was to compare the bacterial adhesion of Staphylococcus spp. on Ti-6Al-4V with respect to Ti-6Al-V modified alloys with a set of Cubic yttria stabilized zirconia (YSZ) and Ag-YSZ nanocomposite films. Silver is well known to have a natural biocidal character and its presence in the surface predicted to enhance the antimicrobial properties of biomedical surfaces. Microbial adhesion tests were performed using collection strains and twelve clinical strains of Staphylococcus aureus and Staphylococcus epidermidis. The adherence study was performed using a previously published protocol by Kinnari et al. Both collection strains and clinical isolates have shown lower bacterial adhesion to materials modified with respect to the alloy Ti-6Al-4V and the modification with silver reduced the bacterial adhesion for most of all the strains studied. Moreover the percentage of dead bacteria have been evaluated, demonstrating increased proportion of dead bacteria for the modified surfaces. Nanocrystalline silver dissolves releasing both Ag+ and Ag-0 whereas other silver sources release only Ag+. We can conclude that YSZ with nanocrystalline silver coating may lead to diminished postoperative infections and to increased corrosion and scratch resistance of YSZ incorporating alloys Ti-6Al-4V.Peer reviewe

Sistematización de la propuesta metodológica de contenidos temáticos para diplomado en formulación y gestión de proyectos

Como punto de partida se desea resolver las siguientes inquietudes que durante el proceso de sistematización serán fundamentales para la comprensión del contenido del documento. El Diplomado: “EMPODERAMIENTO DEL LIDERAZGO COMUNAL PARA LA GESTIÓN DE PROYECTOS DE INVERSIÓN SOCIAL” es una apuesta que está haciendo la corporación Universitaria Minuto de Dios – Regional Soacha para fortalecer y desarrollar capacidades de liderazgo comunitario y gestión de la ciencia, tecnología e innovación en las personas que hacen parte de las JAC del departamento con el propósito de incrementar su competitividad, sustentabilidad y mejorar significativamente la calidad de vida de las y los residentes de Soacha y Sibaté.En este documento, los autores presentan la propuesta metodológica de contenidos temáticos para el diplomado en EMPODERAMIENTO DEL LIDERAZGO COMUNAL PARA LA GESTIÓN DE PROYECTOS DE INVERSIÓN SOCIAL, producto de investigación desarrollada por el semillero HYSCA, en el programa de Especialización en Gerencia de Proyectos de la Corporación Universitaria Minuto de Dios, Centro Regional de Soacha Esta propuesta metodológica tiene como fin desarrollar los contenidos temáticos del Diplomado, cuyo objetivo es; “Contribuir al fortalecimiento de los conocimientos, habilidades y competencias en formulación y gestión de proyectos de inversión social de los líderes comunales”; enfocado al módulo 1, EL ROL DEL LIDER COMUNAL. Soportados en proceso histórico de la investigación y los resultados previos, se presenta de forma detallada el proceso alcanzado de planeación, desarrollo y ejecución de los temas propuestos en el módulo del diplomado a través de la sistematización de la experiencia, incluyendo los avances investigativos durante el proceso académico de la especialización, para cumplir con el objetivo del semillero y el programa de postgrado.In this document, the authors present the methodological proposal of thematic contents for the diploma course in EMPOWERMENT OF COMMUNAL LEADERSHIP FOR THE MANAGEMENT OF SOCIAL INVESTMENT PROJECTS, product of research developed by the HYSCA seed group, in the Project Management Specialization program of the Corporación Universitaria Minuto de Dios, Soacha Regional Center. This methodological proposal aims to develop the thematic contents of the Diploma, whose objective is to "Contribute to the strengthening of knowledge, skills and competencies in the formulation and management of social investment projects of community leaders"; focused on module 1, THE ROLE OF THE COMMUNAL LEADER. Supported by the historical process of research and previous results, we present in detail the achieved process of planning, development and execution of the topics proposed in the diploma module through the systematization of the experience, including the research advances during the academic process of the specialization, to fulfill the objective of the seedbed and the postgraduate program

Sistematización de la propuesta metodológica de contenidos temáticos para diplomado en formulación y gestión de proyectos

Como punto de partida se desea resolver las siguientes inquietudes que durante el proceso de sistematización serán fundamentales para la comprensión del contenido del documento. El Diplomado: “EMPODERAMIENTO DEL LIDERAZGO COMUNAL PARA LA GESTIÓN DE PROYECTOS DE INVERSIÓN SOCIAL” es una apuesta que está haciendo la corporación Universitaria Minuto de Dios – Regional Soacha para fortalecer y desarrollar capacidades de liderazgo comunitario y gestión de la ciencia, tecnología e innovación en las personas que hacen parte de las JAC del departamento con el propósito de incrementar su competitividad, sustentabilidad y mejorar significativamente la calidad de vida de las y los residentes de Soacha y Sibaté.En este documento, los autores presentan la propuesta metodológica de contenidos temáticos para el diplomado en EMPODERAMIENTO DEL LIDERAZGO COMUNAL PARA LA GESTIÓN DE PROYECTOS DE INVERSIÓN SOCIAL, producto de investigación desarrollada por el semillero HYSCA, en el programa de Especialización en Gerencia de Proyectos de la Corporación Universitaria Minuto de Dios, Centro Regional de Soacha Esta propuesta metodológica tiene como fin desarrollar los contenidos temáticos del Diplomado, cuyo objetivo es; “Contribuir al fortalecimiento de los conocimientos, habilidades y competencias en formulación y gestión de proyectos de inversión social de los líderes comunales”; enfocado al módulo 1, EL ROL DEL LIDER COMUNAL. Soportados en proceso histórico de la investigación y los resultados previos, se presenta de forma detallada el proceso alcanzado de planeación, desarrollo y ejecución de los temas propuestos en el módulo del diplomado a través de la sistematización de la experiencia, incluyendo los avances investigativos durante el proceso académico de la especialización, para cumplir con el objetivo del semillero y el programa de postgrado.In this document, the authors present the methodological proposal of thematic contents for the diploma course in EMPOWERMENT OF COMMUNAL LEADERSHIP FOR THE MANAGEMENT OF SOCIAL INVESTMENT PROJECTS, product of research developed by the HYSCA seed group, in the Project Management Specialization program of the Corporación Universitaria Minuto de Dios, Soacha Regional Center. This methodological proposal aims to develop the thematic contents of the Diploma, whose objective is to "Contribute to the strengthening of knowledge, skills and competencies in the formulation and management of social investment projects of community leaders"; focused on module 1, THE ROLE OF THE COMMUNAL LEADER. Supported by the historical process of research and previous results, we present in detail the achieved process of planning, development and execution of the topics proposed in the diploma module through the systematization of the experience, including the research advances during the academic process of the specialization, to fulfill the objective of the seedbed and the postgraduate program

Relationships between physical activity frequency and self-perceived health, self-reported depression, and depressive symptoms in spanish older adults with diabetes: a cross-sectional study

Diabetes is one of the most prevalent noncommunicable diseases in the world. This disease can affect both physical and mental health in the population. This study analyzed the prevalence of Self-Perceived Health (SPH), self-reported depression, and depressive symptoms in comparison with the Physical Activity Frequency (PAF) reported by Spanish older adults with diabetes. A cross-sectional study was carried out with data from 2799 self-reported diabetic participants, all of whom were residents of Spain, aged 50–79 years, and included in the European Health Surveys carried out in Spain (EHIS) both in 2014 and 2020. The relationships between the variables were analysed with a chi-squared test. A z-test for independent proportions was performed to analyze differences in proportions between the sexes. A multiple binary logistic regression was carried out on the prevalence of depression. Linear regressions were performed on depressive symptoms and SPH. Dependent relationships were found between the SPH, self-reported depression, and depressive symptoms with PAF. Most of the very active participants reported a higher prevalence of self-reported depression. Physical inactivity increased the risk of depression, major depressive symptoms, and negative SPH.info:eu-repo/semantics/publishedVersio

Coste de la limpieza “cotidiana” de playas

Más de 90.000 buques mercantes al año cruzan el Estrecho de Gibraltar de los que aproximadamente un 5% son petroleros. Ello, conjuntamente con la existencia de varios puertos con refinerías y polígonos industriales petroquímicos y la práctica del bunkering para aprovisionamiento de combustible, hace a la costa gaditana un punto de riesgo para la contaminación por hidrocarburos (Carmona et al., 2009). La Demarcación de Costas de Andalucía-Atlántico (DCAA), dependiente del Ministerio de Agricultura, Alimentación y Medio Ambiente, ha realizado en numerosas ocasiones la limpieza de su litoral debido a la polución producida por ese tipo de vertidos (Carmona et al., 2012), existiendo ya alguna bibliografía sobre cómo abordar la retirada de alquitrán y otros derivados similares de las playas (e.g. DGC 2005, CEPRECO 2006a, CEPRECO 2006b). Sin embargo, además de la fracción no volátil de los hidrocarburos, existe otro tipo de desechos, naturales y/o antrópicos, que llegan a nuestras playas y que, debido sobre todo al carácter turístico de nuestro litoral, deben ser recogidos. Por ley, esta limpieza corresponde a las autoridades locales. No obstante, debido a la escasez de su presupuesto, los municipios suelen atender prioritariamente a la limpieza de las playas más urbanas y de máxima utilización. Es por este motivo que, dentro de un espíritu de colaboración entre Administraciones, la DCAA, mediante su partida de conservación y mantenimiento, ha apoyado las tareas de los Ayuntamientos, reforzando la labor municipal en las playas más concurridas y limpiando aquellas que son menos visitadas ya sea por su lejanía del casco urbano o por su escasez de servicios. En esta ponencia se presentarán los medios humanos y materiales con los que se cuenta para esta tarea, aportando cifras de toneladas de basura retiradas y coste del trabajo, junto con una comparación superficial con los datos de otros organismos y alguna sugerencia de posibles mejoras de la productividad

Edge detection algorithm based on fuzzy logic theory for a local vision system of robocup humanoid league

Este artículo presenta el desarrollo de un algoritmo para la extracción de bordes basado en la teoría de lógica difusa, mediante el cual es posible reconocer las marcas de un campo de juego para la liga humanoide de RoboCup. El algoritmo propuesto describe la creación de un sistema de inferencia difuso que permite evaluar la relación existente entre los pixeles de una imagen, encontrando así las variaciones en los niveles de gris para una vecindad de pixeles específica. Posteriormente se plantea la implementación del método OTSU para binarizar la imagen obtenida en el proceso difuso y así generar una imagen que contiene solo los bordes extraídos, validando el algoritmo en imágenes de la liga humanoide. Luego se analizan los resultados obtenidos evidenciando el buen desempeño del algoritmo, considerando que el tiempo de procesamiento de la propuesta es tan solo el 35% más del tiempo necesario en métodos tradicionales, mientras que los bordes extraídos son unos 52% menos susceptibles al ruido.At this paper we shown the development of an algorithm to perform edges extraction based on fuzzy logic theory. This method allows recognizing landmarks on the game field for Humanoid League of RoboCup. The proposed algorithm describes the creation of a fuzzy inference system that permit evaluate the existent relationship between image pixels, finding variations on grey levels of related neighbor pixels. Subsequently, it shows an implementation of OTSU method to binarize an image that was obtained from fuzzy process and so generate an imagecontaining only extracted edges, validating the algorithm with Humanoid League images. Later, we analyze obtained results that evidence a good performance of algorithm, considering that this proposal only takes an extra 35% processing time that will be required by traditional methods, whereas extracted edges are 52% less noise susceptible

One-dimensional migration of olfactory ensheathing cells on synthetic materials: Experimental and numerical characterization

Olfactory ensheathing cells (OECs) are of great interest for regenerative purposes since they are believed to aid axonal growth. With the view set on the strategies to achieve reconnection between neuronal structures, it is of great importance to characterize the behaviour of these cells on long thread-like structures that may efficiently guide cell spread in a targeted way. Here, rat OECs were studied on polycaprolactone (PCL) long monofilaments, on long bars and on discs. PCL turns out to be an excellent substrate for OECs. The cells cover long distances along the monofilaments and colonize completely these struc- tures. With the help of a one-dimensional (1D) analytical model, a migration coefficient, a net proliferation rate constant and the fraction of all cells which undergo migration were obtained. The separate effect of the three phenomena summarized by these parameters on the colo- nization patterns of the 1D path was qualitatively dis- cussed. Other features of interest were also determined, such as the speed of the advance front of colonization and the order of the kinetics of net cell proliferation. Charac- terizing migration by means of these quantities may be useful for comparing and predicting features of the colo- nization process (such as times, patterns, advance fronts and proportion of motile cells) of different cell substrate combinations.Support of the Spanish Science & Innovation Ministery through project MAT2008-06434 is acknowledged. MMP and CMR acknowledge partial funding through the "Convenio de Colaboracion para la Investigacion Basica y Traslacional en Medicina Regenerativa" between the Instituto Nacional de Salud Carlos III, the Conselleria de Sanidad of the Generalitat Valenciana and the Foundation Centro de Investigacion Principe Felipe.Perez Garnes, M.; Martínez Ramos, C.; Barcia, JA.; Escobar Ivirico, JL.; Gomez Pinedo, UA.; Vallés Lluch, A.; Monleón Pradas, M. (2013). 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PLGA conduit seeded with olfactory ensheathing cells for bridging sciatic nerve defect of rats. Journal of Biomedical Materials Research, Part A, 94(3), 769–780.Clements, I. P., Kim, Y. T., English, A. W., Lu, X., Chung, A., & Bellamkonda, R. V. (2009). Thin-film enhanced nerve guidance channels for peripheral nerve repair. Biomaterials, 30(23–24), 3834–3846.Martín-López, E., Nieto-Díaz, M., & Nieto-Sampedro, M. (2012). Differential adhesiveness and neurite-promoting activity for neural cells of chitosan, gelatin, and poly-l-lysine films. Journal of Biomaterials Applications, 26(7), 791–809.Cai, J., Peng, X., Nelson, K. D., Eberhart, R., & Smith, G. M. (2005). Permeable guidance channels containing microfilament scaffolds enhance axon growth and maturation. Journal of Biomedical Material Research Part A, 75(2), 374–386.Novikova, L. N., Mosahebi, A., Wiberg, M., Terenghi, G., Kellerth, J. O., & Novikov, L. N. (2006). 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Poly(l-lactide) microfilaments enhance peripheral nerve regeneration across extended nerve lesions. Journal of Neuroscience Research, 72(2), 227–238.Schnell, E., Klinkhammer, K., Balzer, S., Brook, G., Klee, D., Dalton, P., et al. (2007). Guidance of glial cell migration and axonal growth on electrospun nanofibers of poly-e-caprolactone and a collagen/poly-e-caprolactone blend. Biomaterials, 28(19), 3012–3025.Lim, S. H., Liu, X. Y., Song, H., Yarema, K. J., & Mao, H. Q. (2010). The effect of nanofiber-guided cell alignment on the preferential differentiation of neural stem cells. Biomaterials, 31(34), 9031–9039.Wong, D. Y., Hollister, S. J., Krebsbach, P. H., & Nosrat, C. (2007). Poly(epsilon-caprolactone) and poly (l-lactic-co-glycolic acid) degradable polymer sponges attenuate astrocyte response and lesion growth in acute traumatic brain injury. Tissue Engineering, 13(10), 2515–2523.Wong, D. Y., Krebsbach, P. H., & Hollister, S. J. (2008). Brain cortex regeneration affected by scaffold architectures. Journal of Neurosurgery, 109(4), 715–722.Wong, D. Y., Leveque, J. C., Brumblay, H., Krebsbach, P. H., Hollister, S. J., & Lamarca, F. (2008). Macro-architectures in spinal cord scaffold implants influence regeneration. Journal of Neurotrauma, 25(8), 1027–1037.Pierucci, A., de Duek, E. A., & de Oliveira, A. L. (2008). Peripheral nerve regeneration through biodegradable conduits prepared using solvent evaporation. Tissue Engineering Part A, 14(5), 595–606.Vleggeert-Lankamp, C. L., de Ruiter, G. C., Wolfs, J. F., Pego, A. P., van den Berg, R. J., Feirabend, H. K., et al. (2007). Pores in synthetic nerve conduits are beneficial to regeneration. Journal of Biomedical Material Research Part A, 80(4), 965–982.Cai, A. Q., Landman, K. A., & Hughes, B. D. (2007). Multi-scale modeling of a wound-healing cell migration assay. Journal of Theoretical Biology, 245(3), 576–594.Maini, P. K., McElwain, D. L., & Leavesley, D. I. (2004). Traveling wave model to interpret a wound-healing cell migration assay for human peritoneal mesothelial cells. Tissue Engineering, 10(3–4), 475–482.Dokukina, I. V., & Gracheva, M. E. (2010). A model of fibroblast motility on substrates with different rigidities. Biophysical Journal, 98(12), 2794–2803.Schneider, I. C., & Haugh, J. M. (2004). Spatial analysis of 3′ phosphoinositide signaling in living fibroblasts: II. Parameter estimates for individual cells from experiments. Biophysical Journal, 86(1), 599–608.Marcy, Y., Prost, J., Carlier, M.-F., & Sykes, C. C. (2004). Forces generated during actin-based propulsion: A direct measurement by micromanipulation. Proceedings of the National academy of Sciences of the United States of America, 101(16), 5992–5997.Mogilner, A., & Oster, G. (2003). Polymer motors: Pushing out the front and pulling up the back. Current Biology, 13(18), R721–R733.Cheng, G., Youssef, B. B., Markenscoff, P., & Zygourakis, K. (2006). 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New Evidence on Regucalcin, Body Composition, and Walking Ability Adaptations to Multicomponent Exercise Training in Functionally Limited and Frail Older Adults

Background: Regucalcin, or senescence marker protein-30 (SMP30), is a Ca2+-binding protein with multiple functions reported in the literature. Physical exercise has been shown to improve aging markers; nevertheless, SMP30 in humans has not been extensively researched. Older adults experience a decline in functional capacity and body composition. The purpose of this study was to examine the effects of a multicomponent training (MCT) program on SMP30 and its regulation of walking ability and body composition in functionally limited, frail, and pre-frail older adults. Methods: A total of 34 older adults (aged 80.3 +/- 6.1 years) were divided into an intervention group (IG = 20) and control group (CG = 14). The IG performed a supervised MCT (strength, endurance, balance, coordination, and flexibility) program for 6 months, 3 days per week, whereas the CG continued their normal lives without any specific physical training. SMP30 was analyzed in plasma after 3 and 6 months of MCT, while some physical fitness variables (Timed Up and Go (TUG) and 6-min walk test (6MWT)) and body composition (fat mass and lean mass) were measured at baseline, as well as after 3 months and 6 months of MCT. Results: No significant changes were observed in SPM30 between the IG (877.5 a.u. to 940.5 a.u., respectively) and CG (790.4 a.u. to 763.8 a.u., respectively). Moreover, no SMP30 differences were found between groups after 3 and 6 months of MCT. The IG improved significantly in the 6MWT after 3 months (472.2 +/- 84.2 m) compared to baseline (411.2 +/- 75.2 m). The IG also significantly enhanced their TUG performance after 3 months (7.6 +/- 1.6 s) and 6 months (7.3 +/- 1.8 s) of training compared to baseline (9.3 +/- 3.2 s) (all, p < 0.001). There were no significant differences in body composition between the IG and CG through the 6 months of MCT. Conclusions: The present study suggests that MCT did not change SMP30 levels from 3 to 6 months, where there were changes in neither walking ability nor body composition; however, MCT was effective in improving 6MWT and TUG performance from baseline to 3 months