A cDNA microarray approach to decipher sunflower (Helianthus annuus) responses to the necrotrophic fungus Phoma macdonaldii

To identify the genes involved in the partial resistance of sunflower (Helianthus annuus) to the necrotrophic fungus Phoma macdonaldii, we developed a 1000‐element cDNA microarray containing carefully chosen genes putatively involved in primary metabolic pathways, signal transduction and biotic stress responses. A two‐pass general linear model was used to normalize the data and then to detect differentially expressed genes. This method allowed us to identify 38 genes differentially expressed among genotypes, treatments and times, mainly belonging to plant defense, signaling pathways and amino acid metabolism. Based on a set of genes whose differential expression was highly significant, we propose a model in which negative regulation of a dual‐specificity MAPK phosphatase could be implicated in sunflower defense mechanisms against the pathogen. The resulting activation of the MAP kinase cascade could subsequently trigger defense responses (e.g. thaumatin biosynthesis and phenylalanine ammonia lyase activation), under the control of transcription factors belonging to MYB and WRKY families. Concurrently, the activation of protein phosphatase 2A (PP2A), which is implicated in cell death inhibition, could limit pathogen development. The results reported here provide a valuable first step towards the understanding and analysis of the P. macdonaldii–sunflower interaction

Quantum enhanced positioning and clock synchronization

A wide variety of positioning and ranging procedures are based on repeatedly sending electromagnetic pulses through space and measuring their time of arrival. This paper shows that quantum entanglement and squeezing can be employed to overcome the classical power/bandwidth limits on these procedures, enhancing their accuracy. Frequency entangled pulses could be used to construct quantum positioning systems (QPS), to perform clock synchronization, or to do ranging (quantum radar): all of these techniques exhibit a similar enhancement compared with analogous protocols that use classical light. Quantum entanglement and squeezing have been exploited in the context of interferometry, frequency measurements, lithography, and algorithms. Here, the problem of positioning a party (say Alice) with respect to a fixed array of reference points will be analyzed.Comment: 4 pages, 2 figures. Accepted for publication by Natur

Quantum teleportation with nonclassical correlated states in noninertial frames

Quantum teleportation is studied in noninertial frame, for fermionic case, when Alice and Bob share a general nonclassical correlated state. In noninertial frames two fidelities of teleportation are given. It is found that the average fidelity of teleportation from a separable and nonclassical correlated state is increasing with the amount of nonclassical correlation of the state. However, for any particular nonclassical correlated state, the fidelity of teleportation decreases by increasing the acceleration.Comment: 10 pages, 3 figures, expanded version to appear in Quantum Inf. Proces

2.5PN kick from black-hole binaries in circular orbit: Nonspinning case

Using the Multipolar post-Minskowskian formalism, we compute the linear momentum flux from black-hole binaries in circular orbits and having no spins. The total linear momentum flux contains various types of instantaneous (which are functions of the retarded time) and hereditary (which depends on the dynamics of the binary in the past) terms both of which are analytically computed. In addition to the inspiral contribution, we use a simple model of plunge to compute the kick or recoil accumulated during this phase.Comment: To appear in Proceedings of "Relativity and Gravitation - 100 Years after Einstein in Prague" Ed. J. Bicak (2013

Neonatal Auditory Screening in 17 732 Neonates

Introdução: A surdez infantil representa uma das anomalias congénitas mais frequentes. Em Portugal, foi desenvolvido o Grupo de Rastreio e Intervenção na Surdez Infantil (GRISI), cujo principal objetivo é implementar um programa de rastreio nacional através da aplicação de técnicas e métodos definidos e semelhantes nos vários hospitais. Objetivos: Analizar a polupação de recém-nascidos rastreados num hospital periférico, no que concerne à presença de fatores de risco para surdez e resultados dos testes de rastreio. Material e métodos: Foram consultados os processos das crianças nascidas no Hospital Professor Doutor Fernando Fonseca, entre 2010 e 2015. Foram analisados os fatores de risco para surdez e os resultados dos testes de rastreio. Foram calculados a efetividade do rastreio assim como o índice de referenciação para consulta de Otorrinolaringologia. Os dados foram analizados através do programa informático SPSS®, atribuindo-se validade estatística a valores de p<0.05. Resultados: Verificou-se o nascimento de 18019 crianças. O programa de rastreio foi efetuado em 17732 recém-nascidos. A efetividade do rastreio foi de 98,7%. O índice de referenciação para consulta foi de 2,3%. 402 crianças não passaram o rastreio. 354 crianças possuiam fatores de risco para surdez. Discussão: O programa de rastreio foi considerado efetivo e o índice de referenciação foi inferior a 4%. Os principais fatores de risco foram: muito baixo peso ao nascer, baixo índice Apgar e consumo de fármacos ototóxicos. Conclusão: Os autores consideram importante a consciencialização dos resultados do programa de rastreio, para que este possa ser melhorado.info:eu-repo/semantics/publishedVersio

PAKs supplement improves immune status and body composition but not muscle strength in resistance trained individuals

Mixed formula supplements are very popular among recreational and professional weightlifters. They are usually known as PAKs and they are supposed to have a synergistic effect of their different nutrients. The purpose of this study was to determine the effects of chronic (4 weeks) PAKS supplementation in combination with strength training on body composition, immune status and performance measures in recreationally trained individuals with or without PAKs supplementation. Methods: Twelve male subjects (Placebo n = 6 and PAKs supplement n = 6) were recruited for this study. The body composition, one maximum strength repetition tests and immune status were assessed before and after 4 week supplementation. Our data showed that, 4 week PAK supplementation associated with strength exercise not was effective in change strength than compared with placebo group. However, we observed that, PAK supplement was able to improve immune status and reduced body composition when compared with placebo group. These results indicate that, a mixed formula supplement is able to improve immune status and body composition but not maximum strength in recreational strength trained subjects in a 4 weeks period

Community-based management induces rapid recovery of a high-value tropical freshwater fishery

Tropical wetlands are highly threatened socio-ecological systems, where local communities rely heavily on aquatic animal protein, such as fish, to meet food security. Here, we quantify how a ‘win-win’ community-based resource management program induced stock recovery of the world’s largest scaled freshwater fish (Arapaima gigas), providing both food and income. We analyzed stock assessment data over eight years and examined the effects of protected areas, community-based management, and landscape and limnological variables across 83 oxbow lakes monitored along a ~500-km section of the Juruá River of Western Brazilian Amazonia. Patterns of community management explained 71.8% of the variation in arapaima population sizes. Annual population counts showed that protected lakes on average contained 304.8 (±332.5) arapaimas, compared to only 9.2 (±9.8) in open-access lakes. Protected lakes have become analogous to a high-interest savings account, ensuring an average annual revenue of US$10,601 per community and US$1046.6 per household, greatly improving socioeconomic welfare. Arapaima management is a superb window of opportunity in harmonizing the co-delivery of sustainable resource management and poverty alleviation. We show that arapaima management deserves greater attention from policy makers across Amazonian countries, and highlight the need to include local stakeholders in conservation planning of Amazonian floodplains