Simulation of Transport and Gain in Quantum Cascade Lasers

Quantum cascade lasers can be modeled within a hierarchy of different approaches: Standard rate equations for the electron densities in the levels, semiclassical Boltzmann equation for the microscopic distribution functions, and quantum kinetics including the coherent evolution between the states. Here we present a quantum transport approach based on nonequilibrium Green functions. This allows for quantitative simulations of the transport and optical gain of the device. The division of the current density in two terms shows that semiclassical transitions are likely to dominate the transport for the prototype device of Sirtori et al. but not for a recent THz-laser with only a few layers per period. The many particle effects are extremely dependent on the design of the heterostructure, and for the case considered here, inclusion of electron-electron interaction at the Hartree Fock level, provides a sizable change in absorption but imparts only a minor shift of the gain peak.Comment: 12 pages, 5 figures included, to appear in in "Advances in Solid State Physics", ed. by B. Kramer (Springer 2003

Simulations of mid infrared emission of InAsN semiconductors

This paper delivers an approximation to the complex many body problem of luminescence in semiconductors to the case of mid infrared luminescence of dilute nitrides. The results are compared with recent experimental data for InAsN semiconductors

Extrinsic Curvature and the Einstein Constraints

The Einstein initial-value equations in the extrinsic curvature (Hamiltonian) representation and conformal thin sandwich (Lagrangian) representation are brought into complete conformity by the use of a decomposition of symmetric tensors which involves a weight function. In stationary spacetimes, there is a natural choice of the weight function such that the transverse traceless part of the extrinsic curvature (or canonical momentum) vanishes.Comment: 8 pages, no figures; added new section; significant polishing of tex

Spatial connectivity of aquatic macrophytes and flood cycle influence species richness of an ant community of a Brazilian floodplain

Despite the environmental and economic importance of Pantanal, there are few studies quantifying the influence of sazonality and spatial variation on biological diversity in this ecosystem. In this context, the present work aimed to study the assemblage of ants associated with macrophytes during the flood and dry period of Paraguay river, in marginal environments in the Pantanal of Porto Murtinho, Mato Grosso do Sul. We observed a wide variation in the temporal distribution of the diversity of the assemblages of ants, since from 37 species, 36 occurred in the dry and 20 in the flood period. Of the total of macrophyte species observed, in only 12.5% we found a more specific correlation with ants that were nesting in spaces provided by plants representing a total of 10.52% of the species analized.

Ethical Aspects Concerning Endodontic Instrument Fracture

Aim: The aim of this study was to address several ethical aspects concerning the behavior of endodontists and general dentists regarding endodontic instrument fracture during root canal treatment. Methods: The responses of a group of professionals (endodontists and general dentists) to a questionnaire were reviewed and analyzed statistically by Fisher's Exact and chi-square tests at 5% significance level. Results: Forty-six percent of the interviewees responded that they would try to solve the problem without informing the patient about the accident. Only 28.1% of the participants affirmed that they would let the patient know right at the moment of occurrence. Conclusions: The outcomes of this survey demonstrate that most professionals are afraid of informing their patient about an accidental endodontic instrument breakage during treatment and might be subject to lawsuits.72515351538Leite, V.G., Odontologia legal (1962) Bahia: Era NovaCoehn, S., Burns, R.C., (1980) Caminhos da polpa, , 2.ed. Rio de Janeiro: Guanabara Koogan;Frank, A.L., The dilemma of the fractured instrument (1983) J Endod, 9, pp. 515-516Cohen, S., Endodontic treatment: Avoid these malpractice traps (1988) Dent Manag, 28 (36-38), p. 40Imura, N., Zuolo, M.L., (1988) Procedimentos clínicos em endodontia, , São Paulo: Pancast;Walton, R.E., Torabinejad, M., (1997) Principios e prática em endodontia, , 2.ed. São Paulo: Santos;Itoh, A., Higuchi, N., Minami, G., Yasue, T., Yoshida, T., Maseki, T., A survey of filling methods, intracanal medications, and instruments breakage (1999) J Endod, 25, pp. 823-824Ree, M.H., Timmerman, M.F., Wesselink, P.R., Factors influencing referral for specialist endodontic treatment amongst a group of Dutch general practitioners (2003) Int Endod J, 36, pp. 129-134De Deus, Q.D., (1992) Endodontia, , 5.ed. Rio de Janeiro: Medsi;Leonardo, M.R., Leal, J.M., (1998) Endodontia: Tratamento dos canais radiculares, , 3.ed. São Paulo: Panamericana

Therapeutic strategies for IVD regeneration through hyaluronan/SDF-1-based hydrogel and intravenous administration of MSCs

Intervertebral disc (IVD) degeneration involves a complex cascade of events, including degradation of the native extracellular matrix, loss of water content, and decreased cell numbers. Cell recruitment strategies for the IVD have been increasingly explored, aiming to recruit either endogenous or transplanted cells. This study evaluates the IVD therapeutic potential of a chemoattractant delivery system (HAPSDF5) that combines a hyaluronan-based thermoreversible hydrogel (HAP) and the chemokine stromal cell derived factor-1 (SDF-1). HAPSDF5 was injected into the IVD and was combined with an intravenous injection of mesenchymal stem/stromal cells (MSCs) in a pre-clinical in vivo IVD lesion model. The local and systemic effects were evaluated two weeks after treatment. The hydrogel by itself (HAP) did not elicit any adverse effect, showing potential to be administrated by intradiscal injection. HAPSDF5 induced higher cell numbers, but no evidence of IVD regeneration was observed. MSCs systemic injection seemed to exert a role in IVD regeneration to some extent through a paracrine effect, but no synergies were observed when HAPSDF5 was combined with MSCs. Overall, this study shows that although the injection of chemoattractant hydrogels and MSC recruitment are feasible approaches for IVD, IVD regeneration using this strategy needs to be further explored before successful clinical translation.Funding: This research was funded by Portuguese funds through FCT-Fundação para a Ciência e a Tecnologia (IUD/BIM/04293/2019) and by EUROSPINE TRF (2017_05)

A computational approach to forecasting and minimizing electricity costs in the short-term market for distributors in Brazil

In Brazil, the electric power distributors must buy electricity on auctions one, three and five years ahead. If there is inefficiency in the contracting of electric energy, the chamber of Commercialization of Electric Energy, which enables the commercialization, can apply penalties. Thus, this paper proposes a computational approach to forecasting electricity by the class of the consumer using a multi-layer perceptron artificial neural network with a backpropagation algorithm and a prediction using time series techniques through the Bayesian and Akaike selection criteria. The forecast of electricity consumption can serve as support in the purchase of electricity in auctions in the regulated contracting environment and in the process of settlement of differences and for energy management, customer service, and distributor billing. The results show that a multilayer network with a backpropagation algorithm is able to learn the behavior of the data that influences the electric energy consumed by consumption class and can be used to follow the evolution in the demand of each class of consumption and, consequently, to help distributors in the process of contracting of electricity, reduce losses like fines, and reduce the costs of the energy distributor

Sistemas aquosos bifásicos uma ferramenta sustentável para a extração de ácido clavulânico a partir de diferentes fontes

O ácido clavulânico (AC) é um inibidor de β-lactamases que tem vindo a ser largamente utilizado na área médica. Embora seja de extrema importância, o desenvolvimento de processos alternativos de produção e purificação é ainda insignificante, sendo fundamental o estudo de técnicas de extração mais biocompatíveis, como os Sistemas Aquosos Bifásicos (SABs). Assim, este trabalho objetivou o estudo de Sistemas Aquosos Bifásicos baseados em polímeros como uma ferramenta alternativa para a extração de AC. Foram testados dois SPAB compostos por Polietileno Glicol (PEG) com massa molecular (M) de 4000 g/mol e Poliacrilato de Sódio de 8000 g/mol, nos quais foi alterado o eletrólito indutor da formação de fases, em particular, sulfato de sódio (Na2SO4,) e cloreto de sódio (NaCl). Ademais, este trabalho visou também avaliar a eficiência de extração do AC, bem como compreender o efeito dos contaminantes no processo de migração. Para tal, foi avaliada a extração do AC a partir de três fontes distintas: solução pura (99,9%); solução comercial (60%); diretamente a partir do sobrenadante de um meio fermentando de Streptomyces clavuligerus. Os resultados obtidos demonstraram que independentemente da fonte inicial do AC, ambos os SABs poliméricos promoveram uma partição preferencial do AC para a fase rica em PEG, sendo o coeficiente de partição maior nos sistemas com Na2SO4 do que com NaCl. Após identificar a grande capacidade de partição de AC, o SAB com PEG/NaPA/Na2SO4 foi também utilizado para avaliar a partição de proteínas presente no meio fermentado, sendo também obtida uma preferencial partição destas para a fase rica em PEG. Assim, apesar da baixa capacidade de purificação de AC frente a proteínas contaminantes, os SABs estudados demonstraram que podem ser uma técnica alternativa sustentável e bastante econômica para uma etapa inicial de clarificação/concentração de bioprodutos a partir de caldos fermentados

Improved methods for detection of β-galactosidase (lacZ) activity in hard tissue

The ß-galactosidase gene (lacZ) of Escherichia coli is widely used as a reporter gene. The expression of lacZ can be detected by enzyme-based histochemical staining using chromogenic substrates such as 5-bromo-4-chloro-3-indolyl-ß-D: -galactoside (X-gal). Because the enzymatic activity of lacZ is vulnerable to high temperatures and acid treatment for demineralization, detection of lacZ on paraffinized sections is difficult, especially for hard tissues, which require demineralization before sectioning in paraffin. To circumvent this problem, whole-mount X-gal staining before sectioning is performed. However, detection of lacZ activity in the center of larger portions of hard whole adult tissues is challenging. In this study, focusing on fixation procedures, we determined the conditions conducive to improved detection of lacZ activity in deeper areas of whole tissues. We used an annexin a5 (Anxa5)-lacZ reporter mouse model in which the Anxa5 expression in hard tissue is indicated by lacZ activity. We found that lacZ activity could be detected throughout the periodontal ligament of adult mice when fixed in 100% acetone, whereas it was not detected in the periodontal ligament around the root apex fixed in glutaraldehyde and paraformaldehyde. This staining could not be detected in wild-type mice. Acetone maintains the lacZ activity within 48 h of fixation at both 4°C and at room temperature. In conclusion, acetone is the optimal fixative to improve permeability for staining of lacZ activity in large volumes of adult hard tissues