10 research outputs found

    Cell Retention as a Viable Strategy for PHA Production from Diluted VFAs with Bacillus megaterium

    Get PDF
    The production of biodegradable and biocompatible materials such as polyhydroxyalkanoates (PHAs) from waste-derived volatile fatty acids (VFAs) is a promising approach towards implementing a circular bioeconomy. However, VFA solutions obtained via acidification of organic wastes are usually too diluted for direct use in standard batch or fed-batch processes. To overcome these constraints, this study introduces a cell recycle fed-batch system using Bacillus megaterium uyuni S29 for poly(3-hydroxybutyrate) (P3HB) production from acetic acid. The concentrations of dry cell weight (DCW), P3HB, acetate, as well as nitrogen as the limiting substrate component, were monitored during the process. The produced polymer was characterized in terms of molecular weight and thermal properties after extraction with hypochlorite. The results show that an indirect pH-stat feeding regime successfully kept the strain fed without prompting inhibition, resulting in a dry cell weight concentration of up to 19.05 g/L containing 70.21% PHA. After appropriate adaptations the presented process could contribute to an efficient and sustainable production of biopolymers.This work was supported by the European project ‘Volatile—Biowaste-derived volatile fatty acid platform for biopolymers, bioactive compounds and chemical building blocks’ and has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement number 720777

    Avances en la creación de BIDISO TEXTOS. Edición académica digital de relaciones de sucesos

    Get PDF
    Progress is reported on the configuration of a text repository associated with the Biblioteca Digital Siglo de Oro (BIDISO) project –called BIDISO Textos– related to the collection of relaciones de sucesos. Key aspects of our model of Digital Academic Edition of this informative publishing genre of the Modern Age are established. Such aspects are related to the delimitation of our corpus, the recipients of the editions, the objectives, the mechanisms for obtaining the texts and the encoding, based on the TEI scheme. We move forward to remedy the lack of texts of this genre that are susceptible to precise systematic analysis to carry out rigorous statistical and quantitative studies that allow us to advance in the knowledge of the relaciones de sucesos through the application of new technologies.Se informa de los avances en la configuración de un repositorio de textos asociado al proyecto Biblioteca Digital Siglo de Oro (BIDISO) –denominado BIDISO Textos–, en particular, en lo relativo a la colección de relaciones de sucesos. Se establecen aspectos clave de nuestro modelo de Edición Académica Digital para este género editorial informativo de la Edad Moderna relacionados con la delimitación de nuestro corpus, los destinatarios de las ediciones, los objetivos, los mecanismos para la obtención de los textos y la codificación, basada en el esquema TEI. Con ello, se realizan progresos para subsanar la carencia de textos de este género susceptibles de análisis sistemáticos precisos para realizar estudios estadísticos y cuantitativos rigurosos que permitan avanzar en el conocimiento de las relaciones de sucesos mediante la aplicación de nuevas tecnologías.Se informa de los avances en la configuración de un repositorio de textos asociado al proyecto Biblioteca Digital Siglo de Oro (BIDISO) –denominado BIDISO Textos–, en particular, en lo relativo a la colección de relaciones de sucesos. Se establecen aspectos clave de nuestro modelo de Edición Académica Digital para este género editorial informativo de la Edad Moderna relacionados con la delimitación de nuestro corpus, los destinatarios de las ediciones, los objetivos, los mecanismos para la obtención de los textos y la codificación, basada en el esquema TEI. Con ello, se realizan progresos para subsanar la carencia de textos de este género susceptibles de análisis sistemáticos precisos para realizar estudios estadísticos y cuantitativos rigurosos que permitan avanzar en el conocimiento de las relaciones de sucesos mediante la aplicación de nuevas tecnologías

    Cell Retention as a Viable Strategy for PHA Production from Diluted VFAs with Bacillus megaterium

    No full text
    The production of biodegradable and biocompatible materials such as polyhydroxyalkanoates (PHAs) from waste-derived volatile fatty acids (VFAs) is a promising approach towards implementing a circular bioeconomy. However, VFA solutions obtained via acidification of organic wastes are usually too diluted for direct use in standard batch or fed-batch processes. To overcome these constraints, this study introduces a cell recycle fed-batch system using Bacillus megaterium uyuni S29 for poly(3-hydroxybutyrate) (P3HB) production from acetic acid. The concentrations of dry cell weight (DCW), P3HB, acetate, as well as nitrogen as the limiting substrate component, were monitored during the process. The produced polymer was characterized in terms of molecular weight and thermal properties after extraction with hypochlorite. The results show that an indirect pH-stat feeding regime successfully kept the strain fed without prompting inhibition, resulting in a dry cell weight concentration of up to 19.05 g/L containing 70.21% PHA. After appropriate adaptations the presented process could contribute to an efficient and sustainable production of biopolymers

    Towards a Circular Bioeconomy. VOLATILE FATTY ACID PLATFORM FOR BIOWASTE RECYCLING

    Get PDF
    Resources in general are not infinitely available, and also renewable resources if consumed outside their normal replacement cycles become scarce. Therefore, the establishment of a circular bioeconomy must respect natural systems and replacement cycles of organic carbon thereby reducing environmental pressure of human consumption. Upcycling of side and biowaste streams towards added value compounds represents hereby a critical aspect reducing land system change and fertilizer use for biomass supply for the bioeconomy. The development of a Volatile Fatty Acids Platform (VFAP) represents an important cornerstone for the upcycling of heterogenous municipal biowaste streams.This e-book was prepared in the context of the EU funded project VOLATILE in accordance with the grant agreement No 720777 (European Union’s Horizon 2020 research and innovation programme)

    Characteristics and predictors of death among 4035 consecutively hospitalized patients with COVID-19 in Spain

    No full text
    corecore